##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935553.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241320
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08442731642632	31.0	31.0	33.0	28.0	34.0
2	31.089122327200396	31.0	31.0	34.0	28.0	34.0
3	30.903936681584618	31.0	30.0	34.0	27.0	34.0
4	34.75721448698823	35.0	35.0	37.0	32.0	37.0
5	34.77105917454003	35.0	35.0	37.0	32.0	37.0
6	34.955730979612134	36.0	35.0	37.0	32.0	37.0
7	35.390274324548315	37.0	35.0	37.0	33.0	37.0
8	35.03070197248467	37.0	35.0	37.0	32.0	37.0
9	36.87235206364993	39.0	37.0	39.0	32.0	39.0
10	36.3004682579148	37.0	35.0	39.0	32.0	39.0
11	36.56397729156307	38.0	35.0	39.0	32.0	39.0
12	36.65907923089674	39.0	35.0	39.0	33.0	39.0
13	36.65547405934029	39.0	35.0	39.0	33.0	39.0
14	37.63940411072435	39.0	36.0	41.0	33.0	41.0
15	37.88520222111719	40.0	37.0	41.0	33.0	41.0
16	37.85374191944306	39.0	36.0	41.0	33.0	41.0
17	37.7702635504724	39.0	36.0	41.0	33.0	41.0
18	37.77227333001823	39.0	36.0	41.0	33.0	41.0
19	37.65528344107409	39.0	36.0	41.0	33.0	41.0
20	37.72435769932041	39.0	36.0	41.0	33.0	41.0
21	37.64942814520139	39.0	36.0	41.0	33.0	41.0
22	37.77358693850489	39.0	36.0	41.0	33.0	41.0
23	37.83908503232223	40.0	36.0	41.0	33.0	41.0
24	37.82994778717056	40.0	36.0	41.0	33.0	41.0
25	37.37369467926405	39.0	35.0	41.0	32.0	41.0
26	37.49099535885961	39.0	35.0	41.0	33.0	41.0
27	37.53132769766285	39.0	36.0	41.0	33.0	41.0
28	37.39509779545831	39.0	35.0	41.0	33.0	41.0
29	37.35704458809879	39.0	35.0	41.0	33.0	41.0
30	37.097020553621746	39.0	35.0	41.0	32.0	41.0
31	37.13116608652412	39.0	35.0	41.0	32.0	41.0
32	37.14919608818167	39.0	35.0	41.0	32.0	41.0
33	37.15071689043594	39.0	35.0	41.0	32.0	41.0
34	37.065527100944806	39.0	35.0	41.0	32.0	41.0
35	37.094099121498424	40.0	35.0	41.0	32.0	41.0
36	36.96564312945467	39.0	35.0	41.0	31.0	41.0
37	36.96916542350406	39.0	35.0	41.0	32.0	41.0
38	36.79411155312448	39.0	35.0	41.0	31.0	41.0
39	36.708685562738275	39.0	35.0	41.0	31.0	41.0
40	36.611602850986245	39.0	35.0	41.0	31.0	41.0
41	36.38199486159456	39.0	35.0	41.0	30.0	41.0
42	36.44737278302669	39.0	35.0	41.0	30.0	41.0
43	36.423520636499255	39.0	35.0	41.0	31.0	41.0
44	36.44017487153987	39.0	35.0	41.0	31.0	41.0
45	36.42449444720703	39.0	35.0	41.0	31.0	41.0
46	36.2751906182662	39.0	35.0	40.0	30.0	41.0
47	36.24557848499917	38.0	35.0	40.0	30.0	41.0
48	36.170959721531574	38.0	35.0	40.0	30.0	41.0
49	36.12448201558097	38.0	35.0	40.0	30.0	41.0
50	35.98466766119675	38.0	35.0	40.0	30.0	41.0
51	34.809709099950275	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	21.0
10	18.0
11	13.0
12	19.0
13	13.0
14	13.0
15	17.0
16	33.0
17	49.0
18	89.0
19	166.0
20	297.0
21	492.0
22	669.0
23	952.0
24	1260.0
25	1672.0
26	2068.0
27	2416.0
28	2770.0
29	3231.0
30	3933.0
31	5210.0
32	6865.0
33	10041.0
34	17352.0
35	19200.0
36	20611.0
37	30628.0
38	52800.0
39	58384.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35728493286922	20.615365489806067	22.661611138736948	12.365738438587767
2	32.979031990717715	24.966434609646942	23.46800928228079	18.58652411735455
3	32.34336151168573	24.860351400629867	25.344356041770265	17.45193104591414
4	29.592242665340628	24.490717719211005	26.155312448201556	19.76172716724681
5	26.26098126968341	29.988397149013757	23.59895574341124	20.151665837891596
6	24.84211834908006	35.18191612796287	24.26031824962705	15.715647273330019
7	76.64760484004641	6.836979943643295	11.080722691861428	5.434692524448865
8	76.28128625890933	7.696419691695673	9.510193933366484	6.512100116028511
9	69.75012431626057	9.127714238355711	12.314768771755345	8.807392673628376
10	39.2864246643461	28.02254268191613	18.468838057351235	14.222194596386542
11	31.357947952925574	25.976297032985247	23.880324879827615	18.78543013426156
12	29.6278799933698	21.31609481186806	28.966517487153986	20.089507707608156
13	25.22003978120338	25.41604508536383	28.97024697497099	20.393668158461793
14	18.986408088844687	30.454583126139568	27.824879827614783	22.734128957400962
15	18.257500414387533	26.80672965357202	34.45342284104094	20.482347090999504
16	20.196834079230896	25.6592905685397	31.580888446875516	22.56298690535389
17	20.200977954583127	25.17362837725841	27.846427979446382	26.77896568871208
18	22.582463119509367	25.58925907508702	29.122327200397812	22.7059506050058
19	24.82098458478369	26.444969335322394	26.112216144538376	22.621829935355546
20	27.039615448367314	24.579396651748716	28.10086192607326	20.28012597381071
21	24.402038786673298	27.238521465274324	27.58619260732637	20.773247140726006
22	21.930217139068457	25.889690038123653	27.273744405768273	24.906348417039613
23	22.5451682413393	26.229902204541688	27.20246974970993	24.02245980440908
24	22.877921432123323	24.93079728161777	28.45474888115366	23.736532405105255
25	21.70230399469584	27.53149345267694	26.328526437924747	24.43767611470247
26	21.891679098292723	26.840295043925078	26.888363998010938	24.37966185977126
27	23.089673462622244	27.083540527100947	26.472318912647108	23.3544670976297
28	20.174871539864082	27.834410740924913	28.680590087850156	23.31012763136085
29	22.50290071274656	25.192690203878666	28.050721034311287	24.253688049063484
30	21.94057682744903	25.633184153820654	27.624316260566882	24.801922758163432
31	23.700066302005634	26.075750041438756	25.92325542847671	24.3009282280789
32	23.79496104757169	27.645864412398474	25.418945798110393	23.140228741919444
33	22.50994530084535	25.609564064312945	26.561826620255264	25.31866401458644
34	21.301176860600034	25.16368307641306	29.142217802088517	24.39292226089839
35	21.44414056025195	26.001574672633847	26.589176197579977	25.96510856953423
36	22.539781203381402	26.708519807724183	27.49295541190121	23.258743576993204
37	20.804326205867728	26.933946626885465	28.67105917454003	23.59066799270678
38	20.173628377258414	27.53107906514172	25.994530084535057	26.300762473064808
39	22.996436267197083	23.128211503397978	27.99767942980275	25.877672799602188
40	22.77349577324714	23.70338140228742	29.521796784352727	24.001326040112716
41	19.262390187303165	25.263964859937012	29.81808387203713	25.655561080722695
42	21.88960716061661	25.57351234874855	28.538869550803913	23.99801093983093
43	22.52693518978949	24.729404939499418	28.274075915796455	24.469583954914636
44	20.950190618266202	24.397066136250622	28.167578319244157	26.48516492623902
45	21.54773744405768	23.438173379744736	26.625642300679598	28.388446875517985
46	21.50091165257749	26.427150671307807	27.052461461959226	25.01947621415548
47	20.609149676777722	24.305900878501575	30.835405271009446	24.249544173711254
48	20.600861926073264	23.705867727498756	29.498591082380244	26.19467926404774
49	20.921183490800598	22.964114039449694	31.337228576164428	24.77747389358528
50	19.563649925410243	23.330432620586773	30.474888115365488	26.631029338637497
51	19.616277142383556	22.908171722194595	28.165506381568044	29.310044753853802
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	141.0
1	264.0
2	387.0
3	781.5
4	1176.0
5	815.5
6	455.0
7	476.0
8	497.0
9	476.0
10	455.0
11	461.5
12	468.0
13	487.0
14	506.0
15	471.0
16	436.0
17	456.0
18	476.0
19	521.5
20	567.0
21	537.5
22	508.0
23	651.5
24	795.0
25	984.0
26	1500.5
27	1828.0
28	2329.5
29	2831.0
30	3222.0
31	3613.0
32	3931.0
33	4249.0
34	4968.0
35	5687.0
36	6242.5
37	6798.0
38	7212.0
39	7626.0
40	8959.0
41	10292.0
42	11476.5
43	12661.0
44	14236.5
45	15812.0
46	19302.0
47	22792.0
48	23649.0
49	24506.0
50	23983.5
51	23461.0
52	20783.5
53	18106.0
54	16196.5
55	14287.0
56	13324.5
57	12362.0
58	11373.5
59	10385.0
60	10786.0
61	11187.0
62	10026.5
63	8866.0
64	7228.0
65	5590.0
66	4589.5
67	3589.0
68	3068.0
69	2547.0
70	2214.5
71	1882.0
72	1463.0
73	1044.0
74	829.5
75	510.0
76	405.0
77	254.0
78	103.0
79	90.5
80	78.0
81	62.0
82	46.0
83	32.0
84	18.0
85	11.5
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	1.5
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	241320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.29769600530416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.68426747457261	28.219376761147025
2	9.73598788141095	7.457318083872037
3	3.4905864531486688	4.010442565887618
4	1.83510062756979	2.8112050389524286
5	1.3492750486907594	2.583706282115034
6	1.0430642718026402	2.396817503729488
7	0.9381086344946981	2.514917951268026
8	0.8147587102358796	2.496270512182994
9	0.7909543388876866	2.7262555942317257
>10	6.209694871239992	38.494115696999835
>50	0.06816706340618914	1.8046577158959058
>100	0.032460506383899586	2.1411403944969334
>500	0.005410084397316598	1.420934858279463
>1k	0.002164033758926639	0.9228410409414886
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	1191	0.4935355544505221	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC	1036	0.42930548649096634	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	829	0.3435272666998177	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC	773	0.32032156472733303	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG	702	0.29090004972650424	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	579	0.23993038289408256	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT	546	0.22625559423172548	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT	429	0.17777225261064147	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	329	0.1363334990883474	No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	313	0.12970329852478038	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	253	0.10484004641140394	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.28775070445881E-4	0.0	0.0	0.23537212000663021	0.0
2	8.28775070445881E-4	0.0	0.0	1.3355710260235372	0.0
3	8.28775070445881E-4	0.0	0.0	1.7731642632189624	0.0
4	8.28775070445881E-4	0.0	0.0	2.388115365489806	0.0
5	8.28775070445881E-4	0.0	0.0	4.161694016243992	0.0
6	8.28775070445881E-4	0.0	0.0	5.090336482678601	0.0
7	8.28775070445881E-4	0.0	0.0	5.869799436432952	0.0
8	8.28775070445881E-4	0.0	0.0	7.042101773578651	0.0
9	8.28775070445881E-4	0.0	0.0	7.421680755842864	0.0
10	8.28775070445881E-4	0.0	0.0	8.627548483341622	0.0
11	8.28775070445881E-4	0.0	0.0	10.065473230565225	0.0
12	8.28775070445881E-4	0.0	0.0	11.558097132438256	0.0
13	8.28775070445881E-4	0.0	0.0	12.209514337808718	0.0
14	8.28775070445881E-4	0.0	0.0	12.51698988894414	0.0
15	8.28775070445881E-4	0.0	0.0	12.95789822642135	0.0
16	8.28775070445881E-4	0.0	0.0	13.718299353555444	0.0
17	8.28775070445881E-4	0.0	0.0	14.604259903862092	0.0
18	8.28775070445881E-4	0.0	0.0	15.565638985579314	0.0
19	8.28775070445881E-4	0.0	0.0	16.20338140228742	0.0
20	8.28775070445881E-4	0.0	0.0	16.76570528758495	0.0
21	8.28775070445881E-4	0.0	0.0	17.445715232885796	0.0
22	8.28775070445881E-4	0.0	0.0	18.155975468257914	0.0
23	8.28775070445881E-4	0.0	0.0	18.856290402784683	0.0
24	8.28775070445881E-4	0.0	0.0	19.397066136250622	0.0
25	8.28775070445881E-4	0.0	0.0	19.87195425161611	0.0
26	8.28775070445881E-4	0.0	0.0	20.378335819658545	0.0
27	8.28775070445881E-4	0.0	0.0	20.83001823305155	0.0
28	8.28775070445881E-4	0.0	0.0	21.260566882148186	0.0
29	8.28775070445881E-4	0.0	0.0	21.74332836068291	0.0
30	8.28775070445881E-4	0.0	0.0	22.324299685065473	0.0
31	8.28775070445881E-4	0.0	0.0	22.841455329023702	0.0
32	8.28775070445881E-4	0.0	0.0	23.312613956572186	0.0
33	8.28775070445881E-4	0.0	0.0	23.80366318581137	0.0
34	8.28775070445881E-4	0.0	0.0	24.313359854135587	0.0
35	8.28775070445881E-4	0.0	0.0	24.848334162108404	0.0
36	8.28775070445881E-4	0.0	0.0	25.338969003812366	0.0
37	8.28775070445881E-4	0.0	0.0	25.844936184319575	0.0
38	8.28775070445881E-4	0.0	0.0	26.32479695010774	0.0
39	8.28775070445881E-4	0.0	0.0	26.83366484336151	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	25	3.8816186E-5	45.0	5
GAATGAC	25	3.8816186E-5	45.0	1
CGACCGT	20	7.022199E-4	45.0	14
CGTCTCG	20	7.022199E-4	45.0	42
TCTAGAC	20	7.022199E-4	45.0	19
TAGCACG	20	7.022199E-4	45.0	21
GTAGACG	25	3.8816186E-5	45.0	1
ATGACGG	25	3.8816186E-5	45.0	2
TCGTAGG	25	3.8816186E-5	45.0	2
CAGTACG	20	7.022199E-4	45.0	1
CGATAGG	20	7.022199E-4	45.0	2
AGTACGG	20	7.022199E-4	45.0	2
GTCGATG	30	2.1583528E-6	44.999996	1
TGCGTAG	30	2.1583528E-6	44.999996	1
CCGATGA	155	0.0	42.096775	18
CTACGAA	60	3.6379788E-12	41.249996	11
TACGAAT	60	3.6379788E-12	41.249996	12
CGAATAT	55	6.002665E-11	40.909092	14
CGATGAA	165	0.0	40.90909	19
CCCTAGC	50	1.0732037E-9	40.5	21
>>END_MODULE