##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935550.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	153932
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15846607592963	31.0	31.0	33.0	28.0	34.0
2	31.134299560845047	31.0	31.0	34.0	28.0	34.0
3	30.935166177273082	31.0	30.0	34.0	27.0	34.0
4	34.81853675648988	35.0	35.0	37.0	32.0	37.0
5	34.84138450744484	35.0	35.0	37.0	33.0	37.0
6	35.01022529428579	36.0	35.0	37.0	32.0	37.0
7	35.47525530753839	37.0	35.0	37.0	33.0	37.0
8	35.23345373281709	37.0	35.0	37.0	32.0	37.0
9	37.02880492685082	39.0	37.0	39.0	33.0	39.0
10	36.426584465868046	38.0	35.0	39.0	32.0	39.0
11	36.62678325494374	39.0	35.0	39.0	32.0	39.0
12	36.62595171894083	39.0	35.0	39.0	33.0	39.0
13	36.58152301016033	39.0	35.0	39.0	32.0	39.0
14	37.5400566483902	39.0	36.0	41.0	33.0	41.0
15	37.71465322350129	39.0	36.0	41.0	33.0	41.0
16	37.72778239742224	39.0	36.0	41.0	33.0	41.0
17	37.67594782111582	39.0	36.0	41.0	33.0	41.0
18	37.684126757269446	39.0	36.0	41.0	33.0	41.0
19	37.55058727230205	39.0	36.0	40.0	33.0	41.0
20	37.6493711508978	39.0	36.0	41.0	33.0	41.0
21	37.64066600836733	39.0	35.0	41.0	33.0	41.0
22	37.74580983811033	39.0	36.0	41.0	33.0	41.0
23	37.82796949302289	39.0	36.0	41.0	34.0	41.0
24	37.75812046877842	40.0	35.0	41.0	33.0	41.0
25	37.42518124886313	39.0	35.0	41.0	33.0	41.0
26	37.49262661434919	39.0	35.0	41.0	33.0	41.0
27	37.53859496401008	39.0	35.0	41.0	33.0	41.0
28	37.42594782111582	39.0	35.0	41.0	33.0	41.0
29	37.45813735935348	39.0	35.0	41.0	33.0	41.0
30	37.201114777953904	39.0	35.0	41.0	32.0	41.0
31	37.101012135228544	39.0	35.0	41.0	32.0	41.0
32	37.09324896707637	39.0	35.0	41.0	32.0	41.0
33	37.10667697424837	39.0	35.0	41.0	32.0	41.0
34	36.98145284931009	39.0	35.0	41.0	32.0	41.0
35	37.05636254969727	39.0	35.0	41.0	32.0	41.0
36	36.86891614479121	39.0	35.0	41.0	31.0	41.0
37	36.8195436946184	39.0	35.0	41.0	31.0	41.0
38	36.61877972091573	39.0	35.0	41.0	31.0	41.0
39	36.66281215081984	39.0	35.0	41.0	31.0	41.0
40	36.63967855936387	39.0	35.0	41.0	31.0	41.0
41	36.50600265052101	39.0	35.0	40.0	31.0	41.0
42	36.536054881376195	39.0	35.0	40.0	31.0	41.0
43	36.439180937037136	39.0	35.0	40.0	31.0	41.0
44	36.428526881999844	39.0	35.0	40.0	31.0	41.0
45	36.390269729490946	39.0	35.0	40.0	31.0	41.0
46	36.304803419691815	38.0	35.0	40.0	31.0	41.0
47	36.25750980952628	38.0	35.0	40.0	31.0	41.0
48	36.19420913130473	38.0	35.0	40.0	31.0	41.0
49	36.21219109736767	38.0	35.0	40.0	31.0	41.0
50	36.08493360704727	38.0	35.0	40.0	31.0	41.0
51	34.89196528337188	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	4.0
11	8.0
12	4.0
13	8.0
14	3.0
15	8.0
16	18.0
17	17.0
18	39.0
19	70.0
20	165.0
21	264.0
22	444.0
23	552.0
24	727.0
25	1022.0
26	1274.0
27	1476.0
28	1610.0
29	1918.0
30	2450.0
31	3221.0
32	4491.0
33	6771.0
34	12261.0
35	13191.0
36	12666.0
37	19609.0
38	33850.0
39	35773.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26390873892368	20.1231712704311	23.894966608632384	12.717953382012837
2	32.64493412675727	25.762024790167086	24.82589714939064	16.767143933685002
3	31.534053997869187	25.33716186368007	27.249045032871656	15.879739105579088
4	28.736065275576227	24.502377673258323	27.345191383208167	19.41636566795728
5	25.485279214198474	28.430735649507575	25.14616843801159	20.937816698282358
6	23.418132681963463	35.401995686406984	25.945872203310554	15.233999428318997
7	76.26419457942468	6.18779720915729	12.280747342982616	5.267260868435413
8	76.17194605410181	6.812098848842346	10.450718499077514	6.565236597978329
9	70.7045968349661	6.773770236208196	12.8004573448016	9.721175584024115
10	42.585687186549904	23.83000285840501	19.454044643089155	14.130265311955927
11	31.21833017176416	23.338876906686068	24.633604448717616	20.80918847283216
12	27.675856871865502	20.197229945690303	31.21053452173687	20.916378660707323
13	24.687524361406336	24.202245147207858	33.082789803289764	18.027440688096043
14	19.945170594808097	30.11654496790791	28.645116025257906	21.29316841202609
15	16.246784294363746	25.772418990203466	37.676376581867316	20.304420133565472
16	18.96551724137931	24.17950783462828	33.403710729412985	23.451264194579423
17	19.441701530545956	24.02879193410077	29.691032403918616	26.83847413143466
18	20.849465997973134	24.07036873424629	33.10617675337162	21.97398851440896
19	21.835615726424656	26.30447210456565	28.169581373593534	23.69033079541616
20	24.26396070992386	25.663929527323752	29.53901722838656	20.533092534365824
21	23.191409193669934	26.76051763116181	30.01974898006912	20.028324195099135
22	20.080295195281035	24.18015747213055	29.25512563989294	26.484421692695477
23	20.890393160616377	26.51625393030689	28.283267936491434	24.310084972585297
24	22.33778551568225	25.061715562716003	28.948496738819742	23.65200218278201
25	20.21606943325624	27.566068133981236	27.379622170828675	24.83824026193384
26	20.759166385157084	26.595509705584284	28.46256788711899	24.182756022139646
27	22.401449990905075	27.13081100745784	28.62367798768287	21.844061013954214
28	17.728607437050126	25.614557077150952	32.63648883922771	24.020346646571213
29	22.49759634124159	23.60003118260011	30.56284593196996	23.339526544188345
30	23.71501702050256	24.434815373021852	30.28415144349453	21.566016162981054
31	23.704622820466177	25.23062131330717	26.129069978951748	24.9356858872749
32	25.12408076293428	28.282618298989163	25.31247563859366	21.28082529948289
33	25.10459163786607	24.212639347244238	27.54527973390848	23.137489280981214
34	22.101967102356884	23.760491645661723	28.94589818881064	25.191643063170748
35	23.087467193306136	23.59483408258192	28.97318296390614	24.344515760205805
36	27.928565860249982	23.016007068056023	28.330041836655145	20.72538523503885
37	23.87742639607099	24.282800197489802	30.483590156692564	21.356183249746643
38	20.434347634020217	27.856456097497595	28.427487461996204	23.28170880648598
39	21.67970272587896	24.618013148663046	29.914507704700778	23.78777642075722
40	22.786685029753396	22.359223553257284	31.649689473273913	23.204401943715407
41	21.2113140867396	22.944546942805914	29.22914013980199	26.614998830652496
42	24.46859652314009	22.23709170282982	30.05223085518281	23.242080918847282
43	26.3181144921134	22.150689915027414	28.133201673466207	23.397993919392977
44	21.252241249382845	23.527921419847726	30.243873918353557	24.975963412415872
45	22.57815139152353	22.77694046721929	27.42574643349011	27.219161707767064
46	24.844086999454305	23.826105033391368	29.17716913962009	22.152638827534236
47	20.808538835329887	23.02055453057194	32.64558376425954	23.52532286983863
48	21.640724475742534	22.773692279707923	30.121092430423822	25.464490814125718
49	22.34688044071408	19.83083439440792	34.28916664501208	23.533118519865916
50	21.646571213262998	20.803341735311694	31.801055011303692	25.749032040121612
51	20.396668658888338	20.66496894732739	29.19470935218148	29.743653041602787
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	125.0
2	177.0
3	531.0
4	885.0
5	616.5
6	348.0
7	347.0
8	346.0
9	324.0
10	302.0
11	304.5
12	307.0
13	313.5
14	320.0
15	336.0
16	352.0
17	311.5
18	271.0
19	326.5
20	382.0
21	380.0
22	378.0
23	443.5
24	509.0
25	647.0
26	797.0
27	809.0
28	871.5
29	934.0
30	1131.5
31	1329.0
32	1728.0
33	2127.0
34	2696.5
35	3266.0
36	3651.0
37	4036.0
38	4351.5
39	4667.0
40	5492.5
41	6318.0
42	7342.0
43	8366.0
44	9656.0
45	10946.0
46	14252.0
47	17558.0
48	18014.0
49	18470.0
50	18478.0
51	18486.0
52	15616.0
53	12746.0
54	10846.5
55	8947.0
56	8118.0
57	7289.0
58	6553.0
59	5817.0
60	5498.5
61	5180.0
62	4675.5
63	4171.0
64	3527.0
65	2883.0
66	2230.0
67	1577.0
68	1366.0
69	1155.0
70	852.5
71	550.0
72	461.5
73	373.0
74	307.5
75	187.0
76	132.0
77	93.5
78	55.0
79	48.0
80	41.0
81	28.0
82	15.0
83	8.5
84	2.0
85	4.0
86	6.0
87	4.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	153932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.17337525660682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.261190615188	30.575838682015434
2	10.287319143564904	8.471273029649455
3	3.703119329746446	4.574097653509342
4	1.8886381924612252	3.1104643608866254
5	1.334826993168084	2.747966634617883
6	1.011376007825936	2.4985058337447703
7	0.8488616103125641	2.4465348335628723
8	0.7920604616671137	2.608944209131305
9	0.637435112576721	2.3620819582672867
>10	5.148393000836239	33.55702517994959
>50	0.05206771959166285	1.549385442922849
>100	0.025244954953533506	1.970350544396227
>500	0.0047334290537875325	1.4350492425226724
>1k	0.0047334290537875325	2.0924823948236884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	1097	0.7126523399942831	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGC	1068	0.693812852428345	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC	1056	0.6860172024010602	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCG	923	0.599615414598654	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	747	0.4852792141984773	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	539	0.35015461372554113	No Hit
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT	408	0.26505210092768233	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	330	0.2143803757503313	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT	249	0.1617597380661591	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	238	0.15461372554114802	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTATCTGT	208	0.1351246004729361	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC	196	0.12732895044565132	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	192	0.1247304004365564	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTG	156	0.10134345035470209	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27739521347088325	0.0
2	0.0	0.0	0.0	1.8573136190005977	0.0
3	0.0	0.0	0.0	2.4283449834992075	0.0
4	0.0	0.0	0.0	3.2813190239846164	0.0
5	0.0	0.0	0.0	5.840890782943117	0.0
6	0.0	0.0	0.0	6.798456461294598	0.0
7	0.0	0.0	0.0	7.998986565496453	0.0
8	0.0	0.0	0.0	9.613335758646675	0.0
9	0.0	0.0	0.0	10.15058597302705	0.0
10	0.0	0.0	0.0	11.985162279448067	0.0
11	0.0	0.0	0.0	14.163396824571889	0.0
12	0.0	0.0	0.0	16.4234856949822	0.0
13	0.0	0.0	0.0	17.121196372424187	0.0
14	0.0	0.0	0.0	17.399241223397343	0.0
15	0.0	0.0	0.0	17.99755736299145	0.0
16	0.0	0.0	0.0	18.97591144141569	0.0
17	0.0	0.0	0.0	20.180339370631188	0.0
18	0.0	0.0	0.0	21.520541537821895	0.0
19	0.0	0.0	0.0	22.423537665982384	0.0
20	0.0	0.0	0.0	23.13683964347894	0.0
21	0.0	0.0	0.0	24.030740846607593	0.0
22	0.0	0.0	0.0	24.96167138736585	0.0
23	0.0	0.0	0.0	25.926383078242342	0.0
24	0.0	0.0	0.0	26.600706805602474	0.0
25	0.0	0.0	0.0	27.202920770210223	0.0
26	0.0	0.0	0.0	27.736273159576957	0.0
27	0.0	0.0	0.0	28.330041836655145	0.0
28	0.0	0.0	0.0	28.899124288646934	0.0
29	0.0	0.0	0.0	29.456513265597795	0.0
30	0.0	0.0	0.0	30.140581555492034	0.0
31	0.0	0.0	0.0	30.73370059506795	0.0
32	0.0	0.0	0.0	31.31967362211886	0.0
33	6.496375022737313E-4	0.0	0.0	31.876412961567446	0.0
34	6.496375022737313E-4	0.0	0.0	32.3967726008887	0.0
35	6.496375022737313E-4	0.0	0.0	33.05420575318972	0.0
36	6.496375022737313E-4	0.0	0.0	33.61159473014058	0.0
37	6.496375022737313E-4	0.0	0.0	34.17288153210509	0.0
38	6.496375022737313E-4	0.0	0.0	34.72052594652184	0.0
39	6.496375022737313E-4	0.0	0.0	35.33443338617052	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGGA	35	1.2027704E-7	45.000004	4
GCGAAGG	35	1.2027704E-7	45.000004	2
GCGCGAC	30	2.1532232E-6	45.000004	9
CGACCTC	30	2.1532232E-6	45.000004	12
AGGGTAC	35	1.2027704E-7	45.000004	6
CGCGACC	30	2.1532232E-6	45.000004	10
ACGGGAT	30	2.1532232E-6	45.000004	5
GGGCGCG	30	2.1532232E-6	45.000004	7
ACGTGGC	30	2.1532232E-6	45.000004	26
TCAGACG	30	2.1532232E-6	45.000004	22
TCGTCCC	30	2.1532232E-6	45.000004	38
CGGGACA	35	1.2027704E-7	45.000004	6
GACGGGA	45	3.8016879E-10	45.000004	4
CCCCATT	30	2.1532232E-6	45.000004	42
GGCGCGA	30	2.1532232E-6	45.000004	8
CTAATCC	35	1.2027704E-7	45.000004	11
TTGATAG	20	7.0142373E-4	45.0	31
AAACTCA	20	7.0142373E-4	45.0	20
CTATGCT	20	7.0142373E-4	45.0	36
CGGCGGG	20	7.0142373E-4	45.0	3
>>END_MODULE