##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935549.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	158516
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.194863609982587	31.0	31.0	33.0	28.0	34.0
2	31.187034747281032	31.0	31.0	34.0	28.0	34.0
3	31.01807388528603	31.0	31.0	34.0	27.0	34.0
4	34.830654318806936	35.0	35.0	37.0	32.0	37.0
5	34.86150924827778	35.0	35.0	37.0	33.0	37.0
6	35.052234474753334	36.0	35.0	37.0	32.0	37.0
7	35.52665976936082	37.0	35.0	37.0	33.0	37.0
8	35.31605642332635	37.0	35.0	37.0	33.0	37.0
9	37.18360922556713	39.0	37.0	39.0	34.0	39.0
10	36.42027303237528	38.0	35.0	39.0	32.0	39.0
11	36.68251785308738	39.0	35.0	39.0	32.0	39.0
12	36.673622851951855	39.0	35.0	39.0	33.0	39.0
13	36.59429962905953	39.0	35.0	39.0	33.0	39.0
14	37.568895253475986	39.0	36.0	41.0	33.0	41.0
15	37.822137828358024	40.0	36.0	41.0	33.0	41.0
16	37.81232178455172	39.0	36.0	41.0	33.0	41.0
17	37.75301546847006	39.0	36.0	41.0	33.0	41.0
18	37.74295339271745	39.0	36.0	41.0	33.0	41.0
19	37.62989225062454	39.0	36.0	41.0	33.0	41.0
20	37.73397007242171	39.0	36.0	41.0	33.0	41.0
21	37.685104342779276	39.0	35.0	41.0	33.0	41.0
22	37.8006825809382	39.0	36.0	41.0	33.0	41.0
23	37.8382245325393	40.0	36.0	41.0	34.0	41.0
24	37.79327638850337	40.0	35.0	41.0	33.0	41.0
25	37.38384137878826	39.0	35.0	41.0	33.0	41.0
26	37.49035428600268	39.0	35.0	41.0	33.0	41.0
27	37.532122940271016	39.0	35.0	41.0	33.0	41.0
28	37.482519114789675	39.0	35.0	41.0	33.0	41.0
29	37.463700825153296	39.0	35.0	41.0	33.0	41.0
30	37.189356279492294	39.0	35.0	41.0	32.0	41.0
31	37.18928688586641	39.0	35.0	41.0	33.0	41.0
32	37.17255040500643	39.0	35.0	41.0	33.0	41.0
33	37.138566453859546	39.0	35.0	41.0	33.0	41.0
34	37.08274874460622	39.0	35.0	41.0	32.0	41.0
35	37.13541219813773	39.0	35.0	41.0	33.0	41.0
36	37.02031340684852	39.0	35.0	41.0	32.0	41.0
37	37.03123344015746	39.0	35.0	41.0	32.0	41.0
38	36.831903404072776	39.0	35.0	41.0	32.0	41.0
39	36.75770900098413	39.0	35.0	41.0	31.0	41.0
40	36.70448408993414	39.0	35.0	41.0	31.0	41.0
41	36.58054076560095	39.0	35.0	41.0	31.0	41.0
42	36.67431678821065	39.0	35.0	41.0	31.0	41.0
43	36.606979737061245	39.0	35.0	41.0	31.0	41.0
44	36.55201367685281	39.0	35.0	41.0	31.0	41.0
45	36.51182215044538	39.0	35.0	40.0	31.0	41.0
46	36.39383406091498	39.0	35.0	40.0	31.0	41.0
47	36.364379620984636	39.0	35.0	40.0	31.0	41.0
48	36.33688712811325	38.0	35.0	40.0	31.0	41.0
49	36.36934441949078	38.0	35.0	40.0	31.0	41.0
50	36.24855535087941	38.0	35.0	40.0	31.0	41.0
51	35.10706174771001	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	8.0
11	9.0
12	5.0
13	2.0
14	9.0
15	7.0
16	10.0
17	22.0
18	50.0
19	80.0
20	139.0
21	262.0
22	410.0
23	523.0
24	725.0
25	937.0
26	1133.0
27	1437.0
28	1690.0
29	1905.0
30	2496.0
31	3270.0
32	4505.0
33	6562.0
34	12600.0
35	14133.0
36	12563.0
37	19608.0
38	34604.0
39	38799.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.273966034974386	20.742385626687526	24.680158469807463	12.303489868530622
2	32.883746751116604	24.653662721744176	25.53496177042065	16.927628756718565
3	32.01443387418305	23.369249791819122	27.218703474728102	17.397612859269724
4	28.412273839864742	24.415200989174593	27.639481188018877	19.533043982941788
5	24.857427641373743	28.489868530621514	25.902747987584853	20.749955840419894
6	24.448005248681522	34.57253526457897	25.372833026319107	15.6066264604204
7	78.16308763784097	5.76913371520856	10.991319488253552	5.076459158696914
8	78.92641752252139	5.695324131317974	9.624265058416816	5.753993287743824
9	73.72757324181785	6.22460824143935	12.000050468091548	8.047768048651239
10	41.228014837618915	24.55588079436776	19.761412097201543	14.454692270811778
11	30.926215650155193	24.70917762244821	24.899694668046127	19.464912059350475
12	29.067728178858914	20.458502611723738	30.192535769259887	20.28123344015746
13	23.92439879885942	25.65166923212799	31.122410356052388	19.301521612960208
14	18.809457720356303	29.796361250599308	29.020414343031618	22.373766686012768
15	17.97862676322895	25.095889373943326	36.330086552777004	20.59539731005072
16	20.651543061899112	23.79570516540917	32.705846728405966	22.84690504428575
17	20.881803729591965	23.772363673067705	28.921370713366475	26.42446188397386
18	21.440106992354085	24.762799969719147	31.562113603674074	22.234979434252693
19	23.49100406268137	25.891392666986295	27.08811728784476	23.52948598248757
20	24.499104191375004	25.82326074339499	29.14784627419314	20.529788791036868
21	23.145928486714276	27.755557798581847	29.332685659491787	19.765828055212094
22	20.738600519821343	24.655555275177267	29.124504781851673	25.481339423149713
23	21.107017588129906	25.548840495596657	29.27780160993212	24.066340306341317
24	23.208382750006308	23.49605087183628	30.121249589946757	23.174316788210653
25	20.05917383734134	26.732317242423477	28.078553584496202	25.12995533573898
26	18.672563022029323	28.775013247874032	29.04312498422872	23.509298745867923
27	20.226980241742158	27.926518458704486	29.994448509929594	21.85205278962376
28	17.43798733250902	28.407227030709837	31.360241237477602	22.79454439930354
29	19.01826944914078	25.841555426581543	30.97352948598249	24.166645638295186
30	21.573216583814883	26.22952888036539	30.024098513714705	22.173156022105022
31	22.745968861187514	28.348557874283987	25.1015670342426	23.8039062302859
32	23.043099750182947	28.261500416361756	25.16149789295718	23.53390194049812
33	22.39016881576623	26.48313104039971	26.14751823159807	24.97918191223599
34	21.542304877741046	26.03270332332383	27.388402432562014	25.036589366373114
35	18.892099220267987	25.4220394155795	28.099371672260215	27.586489691892304
36	21.60917509904363	29.311867571727774	26.85533321557445	22.223624113654143
37	20.830073935754122	27.122183249640415	29.317545232027054	22.730197582578416
38	19.414443967801358	28.22428019884428	25.326150041636176	27.035125791718183
39	21.42685911832244	26.114713972091145	26.66797042569835	25.790456483888065
40	22.898634838123595	24.458729718135707	27.825582275606248	24.817053168134446
41	19.252315223699814	26.3203714451538	27.195361982386636	27.231951348759747
42	20.980216508112743	25.830200105982993	27.60036841706831	25.589214968835954
43	21.918292159781977	25.80181180448661	27.033233238285092	25.246662797446312
44	20.26356960811527	26.20114057886901	27.230689646471017	26.304600166544702
45	21.122788866739004	24.27073607711524	26.21501930404502	28.391455752100736
46	23.080319967700422	25.601201140578866	26.471144868656793	24.84733402306392
47	19.207524792449973	25.340659617956547	30.26445279971738	25.1873627898761
48	19.33684927704459	24.162860531429004	29.13207499558404	27.368215195942362
49	19.869287642887784	23.282192333896894	31.275076332988466	25.573443690226856
50	19.770874864367006	23.22793913548159	30.21966236846754	26.781523631683868
51	19.028363067450606	23.73892856241641	27.698150344444727	29.534558025688256
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	119.0
1	186.0
2	253.0
3	545.0
4	837.0
5	593.5
6	350.0
7	345.5
8	341.0
9	350.5
10	360.0
11	380.0
12	400.0
13	376.0
14	352.0
15	376.0
16	400.0
17	383.0
18	366.0
19	351.5
20	337.0
21	366.5
22	396.0
23	506.5
24	617.0
25	614.5
26	886.5
27	1161.0
28	1346.0
29	1531.0
30	1964.0
31	2397.0
32	2440.5
33	2484.0
34	3167.0
35	3850.0
36	4039.5
37	4229.0
38	4556.0
39	4883.0
40	5777.5
41	6672.0
42	8006.5
43	9341.0
44	10681.5
45	12022.0
46	14130.5
47	16239.0
48	16824.0
49	17409.0
50	17033.0
51	16657.0
52	14525.5
53	12394.0
54	10773.0
55	9152.0
56	8265.5
57	7379.0
58	6844.5
59	6310.0
60	5813.0
61	5316.0
62	5058.0
63	4800.0
64	3933.5
65	3067.0
66	2645.5
67	2224.0
68	1792.5
69	1361.0
70	1053.0
71	745.0
72	579.0
73	413.0
74	434.5
75	300.0
76	144.0
77	119.0
78	94.0
79	64.5
80	35.0
81	20.5
82	6.0
83	3.0
84	0.0
85	1.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	158516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.60115067248732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.33689061759466	28.694895152539807
2	9.759924169376848	7.534886068283328
3	3.371521025020837	3.904337732468647
4	1.7323375116442497	2.674808852103258
5	1.2208076615077874	2.35622902419945
6	0.8661687558221248	2.0061066390774434
7	0.79099184493945	2.1373236771051505
8	0.709277811371325	2.190315173231724
9	0.6471751458595498	2.24835347851321
>10	6.488094265309124	41.492341467107416
>50	0.049028420140874995	1.2781044184814152
>100	0.019611368056349998	1.4528501854702363
>500	0.008171403356812499	2.0294481314189103
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	898	0.5665043276388503	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGC	636	0.4012213278154887	No Hit
GAATCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTC	574	0.36210855686492216	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCG	568	0.3583234499987383	No Hit
CCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	541	0.34129046910091093	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	467	0.29460748441797674	No Hit
CTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGCT	382	0.2409851371470388	No Hit
TCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	254	0.16023619066844988	No Hit
ACTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	177	0.11166065255242372	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCC	159	0.10030533195387217	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.308511443639758E-4	0.0	0.0	0.2750510989426935	0.0
2	6.308511443639758E-4	0.0	0.0	1.6162406318605063	0.0
3	6.308511443639758E-4	0.0	0.0	2.112720482474955	0.0
4	6.308511443639758E-4	0.0	0.0	2.852708874813899	0.0
5	6.308511443639758E-4	0.0	0.0	4.848721895581519	0.0
6	6.308511443639758E-4	0.0	0.0	5.7388528602790885	0.0
7	6.308511443639758E-4	0.0	0.0	6.669989149360317	0.0
8	6.308511443639758E-4	0.0	0.0	8.080572308158168	0.0
9	6.308511443639758E-4	0.0	0.0	8.554341517575512	0.0
10	6.308511443639758E-4	0.0	0.0	10.007822554190113	0.0
11	6.308511443639758E-4	0.0	0.0	11.718690857705216	0.0
12	6.308511443639758E-4	0.0	0.0	13.413787882611219	0.0
13	6.308511443639758E-4	0.0	0.0	13.985969870549345	0.0
14	6.308511443639758E-4	0.0	0.0	14.220015645108381	0.0
15	6.308511443639758E-4	0.0	0.0	14.67549017133917	0.0
16	6.308511443639758E-4	0.0	0.0	15.592747735244393	0.0
17	6.308511443639758E-4	0.0	0.0	16.67339574553988	0.0
18	6.308511443639758E-4	0.0	0.0	17.902293774760906	0.0
19	6.308511443639758E-4	0.0	0.0	18.62777259077948	0.0
20	6.308511443639758E-4	0.0	0.0	19.275656716041283	0.0
21	6.308511443639758E-4	0.0	0.0	20.039617451866057	0.0
22	6.308511443639758E-4	0.0	0.0	20.787806909081734	0.0
23	6.308511443639758E-4	0.0	0.0	21.5921421181458	0.0
24	6.308511443639758E-4	0.0	0.0	22.208483686189407	0.0
25	6.308511443639758E-4	0.0	0.0	22.741552903176967	0.0
26	6.308511443639758E-4	0.0	0.0	23.24812637210124	0.0
27	6.308511443639758E-4	0.0	0.0	23.764162608190972	0.0
28	0.0012617022887279517	0.0	0.0	24.252441393928688	0.0
29	0.0012617022887279517	0.0	0.0	24.77983295061697	0.0
30	0.0012617022887279517	0.0	0.0	25.34570642711146	0.0
31	0.0012617022887279517	0.0	0.0	25.9456458654016	0.0
32	0.0012617022887279517	0.0	0.0	26.482500189255344	0.0
33	0.0012617022887279517	0.0	0.0	26.992227913901434	0.0
34	0.0018925534330919277	0.0	0.0	27.486815211082792	0.0
35	0.0018925534330919277	0.0	0.0	28.034394004390723	0.0
36	0.0018925534330919277	0.0	0.0	28.52582704585026	0.0
37	0.0018925534330919277	0.0	0.0	29.08413030861238	0.0
38	0.0018925534330919277	0.0	0.0	29.5478058997199	0.0
39	0.0018925534330919277	0.0	0.0	30.07267405183073	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAC	30	2.1536325E-6	45.000004	39
CGGAACG	20	7.014874E-4	45.000004	27
AGCCGGC	20	7.014874E-4	45.000004	20
GTACCGG	20	7.014874E-4	45.000004	2
GGCACTA	20	7.014874E-4	45.000004	8
CGGCCAA	20	7.014874E-4	45.000004	15
TTGTACG	20	7.014874E-4	45.000004	24
TACGGAC	20	7.014874E-4	45.000004	27
CGACACA	20	7.014874E-4	45.000004	38
AGGCGAC	20	7.014874E-4	45.000004	35
TATGCAG	40	6.7520887E-9	45.000004	1
GCCGATG	20	7.014874E-4	45.000004	9
CCTCCGC	30	2.1536325E-6	45.000004	45
GTGCTAG	30	2.1536325E-6	45.000004	1
GGAACGC	20	7.014874E-4	45.000004	28
TGTTACG	20	7.014874E-4	45.000004	1
TAGGGCG	20	7.014874E-4	45.000004	5
CCGACGG	20	7.014874E-4	45.000004	2
CGTAAGG	30	2.1536325E-6	45.000004	2
CATCGAA	20	7.014874E-4	45.000004	42
>>END_MODULE