##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935548.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	429232
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.235331941700526	31.0	31.0	33.0	28.0	34.0
2	31.213672326387595	31.0	31.0	34.0	28.0	34.0
3	31.013680247511836	31.0	31.0	34.0	27.0	34.0
4	34.869224568531706	35.0	35.0	37.0	32.0	37.0
5	34.8745177433183	35.0	35.0	37.0	33.0	37.0
6	35.08206750661647	36.0	35.0	37.0	32.0	37.0
7	35.54392729339844	37.0	35.0	37.0	33.0	37.0
8	35.29170238938383	37.0	35.0	37.0	32.0	37.0
9	37.19054730309017	39.0	37.0	39.0	34.0	39.0
10	36.4941942818802	38.0	35.0	39.0	32.0	39.0
11	36.72052875834048	39.0	35.0	39.0	32.0	39.0
12	36.904655291310995	39.0	37.0	39.0	33.0	39.0
13	36.96729507585641	39.0	37.0	39.0	33.0	39.0
14	38.04434897677713	40.0	37.0	41.0	33.0	41.0
15	38.176580497260225	40.0	37.0	41.0	33.0	41.0
16	38.17920145748686	40.0	37.0	41.0	34.0	41.0
17	38.051617773139	40.0	37.0	41.0	33.0	41.0
18	38.059734595743095	39.0	37.0	41.0	34.0	41.0
19	37.92995396428971	39.0	37.0	41.0	34.0	41.0
20	37.94276987736236	40.0	37.0	41.0	33.0	41.0
21	37.95114530137548	40.0	37.0	41.0	34.0	41.0
22	38.08621444813061	40.0	37.0	41.0	34.0	41.0
23	38.114448596563165	40.0	37.0	41.0	34.0	41.0
24	38.11876094978939	40.0	37.0	41.0	34.0	41.0
25	37.74059250009319	40.0	36.0	41.0	33.0	41.0
26	37.82699099787528	40.0	36.0	41.0	33.0	41.0
27	37.86824840645618	40.0	37.0	41.0	33.0	41.0
28	37.76539260819324	40.0	36.0	41.0	33.0	41.0
29	37.75156558690871	40.0	36.0	41.0	33.0	41.0
30	37.519513922540725	40.0	36.0	41.0	33.0	41.0
31	37.49957598687889	40.0	36.0	41.0	33.0	41.0
32	37.44665821746748	40.0	36.0	41.0	33.0	41.0
33	37.41670238938383	40.0	36.0	41.0	33.0	41.0
34	37.30434823125955	40.0	36.0	41.0	32.0	41.0
35	37.33083274313192	40.0	36.0	41.0	33.0	41.0
36	37.17777798486599	40.0	36.0	41.0	32.0	41.0
37	37.186200003727585	40.0	36.0	41.0	32.0	41.0
38	37.05827617698587	40.0	36.0	41.0	32.0	41.0
39	36.97979880344429	40.0	36.0	41.0	31.0	41.0
40	36.90551496626533	39.0	35.0	41.0	31.0	41.0
41	36.76155785216387	39.0	35.0	41.0	31.0	41.0
42	36.81245573489395	39.0	35.0	41.0	31.0	41.0
43	36.759165206694746	39.0	35.0	41.0	31.0	41.0
44	36.811109143773066	39.0	35.0	41.0	31.0	41.0
45	36.80132888507847	39.0	35.0	41.0	31.0	41.0
46	36.70133634025422	39.0	35.0	41.0	31.0	41.0
47	36.62898152980207	39.0	35.0	41.0	31.0	41.0
48	36.595684851082865	39.0	35.0	41.0	31.0	41.0
49	36.6104274611399	39.0	35.0	41.0	31.0	41.0
50	36.49489087486487	39.0	35.0	41.0	31.0	41.0
51	35.41146978790025	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	18.0
9	20.0
10	20.0
11	18.0
12	11.0
13	12.0
14	17.0
15	26.0
16	32.0
17	52.0
18	97.0
19	229.0
20	430.0
21	863.0
22	1260.0
23	1638.0
24	2188.0
25	2925.0
26	3584.0
27	3947.0
28	4115.0
29	4915.0
30	6011.0
31	7965.0
32	10581.0
33	15286.0
34	25717.0
35	29733.0
36	34055.0
37	53441.0
38	98140.0
39	121864.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60625489245909	20.823004808588365	24.39566481529802	11.175075483654528
2	32.200534908860476	27.330208372162375	23.485667424609534	16.983589294367615
3	30.617008983486787	26.446071122376708	26.84632087076453	16.090599023371976
4	27.000083870727252	24.59159615312931	29.50315913072651	18.90516084541693
5	26.631052670816715	29.459592947403735	24.736972080366794	19.172382301412753
6	23.826741715435944	35.66952137771648	25.519998508964846	14.983738397882728
7	77.89726767808551	5.1736590002609315	12.305466507622917	4.623606814030641
8	78.46572482946286	7.369907183061841	9.272607820479367	4.891760166995937
9	72.85314235658106	7.122488537667275	10.772495992843032	9.251873112908637
10	36.34491370634063	32.77458344205464	17.708372162373728	13.172130689230999
11	26.20680657546502	24.961093301524585	29.869627613971	18.9624725090394
12	22.963572147463378	22.86735378536549	33.02107019047974	21.148003876691394
13	22.873178141424685	24.78473180005219	34.44104819771126	17.90104186081187
14	19.143027546874418	29.916455436686917	30.36446490476013	20.576052111678532
15	17.318140306407724	27.181104857047007	35.522980579267156	19.977774257278117
16	20.08866999664517	28.287732508293885	29.7279792746114	21.89561822044955
17	20.41460096171767	27.634472732694675	29.589825548887315	22.36110075670034
18	21.771908897752265	25.944710552801283	31.168226040928914	21.115154508517538
19	20.66481529802065	27.877930815968988	29.190740671711335	22.266513214299028
20	22.950525589890784	27.38169567972565	31.143996719722665	18.523782010660902
21	21.242358444850336	29.753140492787118	30.726506877399633	18.27799418496291
22	20.841875722220152	24.416166548626382	31.463870354493608	23.278087374659858
23	19.479209378611102	27.866282103850597	30.716023036493084	21.938485481045216
24	19.834029149737205	26.128993178514182	31.20130838334514	22.835669288403473
25	18.49046669400231	31.044050769746896	28.319417005255897	22.146065530994893
26	18.87813583330227	28.166818876505012	29.706312670071195	23.24873262012152
27	20.973506169157936	28.603179632459835	28.962658888433296	21.46065530994893
28	17.429734968501883	28.805168300592687	31.171254706079697	22.593842024825737
29	19.01652253326872	28.3627502143363	29.910864054870096	22.709863197524882
30	20.300676557199836	30.260558392664105	29.45679725649532	19.981967793640734
31	23.267836507995675	28.272356208297612	25.85897603160994	22.600831252096768
32	22.1120512916092	30.95435568643531	26.71329257837253	20.220300443582957
33	22.608053453610168	27.55968800089462	26.897575204085438	22.934683341409773
34	20.69719871770977	27.39730495396429	28.03588735229433	23.869608976031607
35	20.745424385879897	29.016708912662615	27.33486785700973	22.902998844447758
36	22.105295038580536	30.133354456331308	27.718809408431806	20.042541096656354
37	19.990121892123607	30.387529354754538	28.70918291273717	20.913165840384686
38	23.302316695866104	28.24533119618295	26.441178663286983	22.01117344466396
39	22.078503000708242	27.004510381332242	28.2059585492228	22.71102806873672
40	24.10281619264174	25.056612740895368	28.84104633391732	21.99952473254557
41	20.165551496626534	26.809976888955156	28.027966228053824	24.996505386364483
42	22.03377194617363	26.01669959369292	29.96328325940284	21.986245200730608
43	22.137678458269654	26.648991687479036	29.070292988407203	22.143036865844113
44	21.26891750848026	26.759188504118985	28.041944682595894	23.92994930480486
45	21.935456815894437	25.507184925634625	26.92320237074589	25.63415588772505
46	23.937870429045365	26.91598016923249	26.84422410258322	22.30192529913893
47	19.75109031945428	26.13947701942073	31.767202818056433	22.34222984306855
48	20.833255675252545	26.066323107317256	29.284862265627915	23.815558951802288
49	21.601837700823797	23.995182092667836	32.221036642188835	22.18194356431953
50	20.841875722220152	24.30294106683565	30.903334327356767	23.95184888358743
51	20.696499794982667	23.55276400641145	27.73371975994334	28.01701643866254
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	133.0
1	302.0
2	471.0
3	1707.5
4	2944.0
5	2008.5
6	1073.0
7	1054.0
8	1035.0
9	1015.0
10	995.0
11	1022.0
12	1049.0
13	1038.5
14	1028.0
15	994.5
16	961.0
17	1024.0
18	1087.0
19	1062.0
20	1037.0
21	1318.5
22	1600.0
23	1718.5
24	1837.0
25	2547.0
26	3771.5
27	4286.0
28	5351.5
29	6417.0
30	7381.5
31	8346.0
32	9570.5
33	10795.0
34	12519.5
35	14244.0
36	15330.0
37	16416.0
38	17713.5
39	19011.0
40	21089.0
41	23167.0
42	24648.5
43	26130.0
44	28294.0
45	30458.0
46	41697.5
47	52937.0
48	49696.5
49	46456.0
50	44824.0
51	43192.0
52	36248.0
53	29304.0
54	24975.5
55	20647.0
56	18304.0
57	15961.0
58	13943.5
59	11926.0
60	10846.0
61	9766.0
62	8644.0
63	7522.0
64	6287.0
65	5052.0
66	4222.0
67	3392.0
68	2727.5
69	2063.0
70	1704.5
71	1346.0
72	1045.0
73	744.0
74	629.0
75	412.5
76	311.0
77	259.5
78	208.0
79	139.0
80	70.0
81	40.0
82	10.0
83	7.5
84	5.0
85	7.0
86	9.0
87	10.5
88	12.0
89	7.5
90	3.0
91	3.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	429232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.89460320148183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74635164281244	28.68335084704137
2	9.718093330628461	7.559627679395218
3	3.534446613518539	4.124126957088743
4	1.9979316329574028	3.1083503235026724
5	1.4992122502077232	2.9155632793315043
6	1.1843552069985477	2.763901549549043
7	1.01720698915793	2.76947035519502
8	0.8600554979036575	2.6761213857772517
9	0.8082478349577615	2.829283094622501
>10	5.525401807923968	33.60928704233865
>50	0.07065317540687113	1.9654266493020136
>100	0.028985918115639442	1.8383597495323682
>500	0.006038732940758217	1.6746524867977242
>1k	0.0030193664703791084	3.4824786005259263
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTC	4061	0.9461083982554889	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGC	3679	0.8571122376710032	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCG	3308	0.7706787937525628	No Hit
GCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	2061	0.48015991351996123	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1718	0.40024974838781824	No Hit
CCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	992	0.23111044842882172	No Hit
CTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGCT	823	0.19173780146866962	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCT	784	0.18265180601632683	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCT	774	0.18032206359264918	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	737	0.17170201662504192	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGAGCGTT	693	0.16145114996086032	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTG	636	0.1481716181458978	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTC	589	0.1372218287546129	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTA	580	0.135125060573303	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTT	522	0.12161255451597272	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCC	443	0.10320758936891937	No Hit
TCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	443	0.10320758936891937	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6594848473552764E-4	0.0	0.0	0.198261080254967	0.0
2	4.6594848473552764E-4	0.0	0.0	1.129459126998919	0.0
3	4.6594848473552764E-4	0.0	0.0	1.5497446602303648	0.0
4	4.6594848473552764E-4	0.0	0.0	2.2242050918850413	0.0
5	4.6594848473552764E-4	0.0	0.0	4.472872479218697	0.0
6	4.6594848473552764E-4	0.0	0.0	5.435755022924665	0.0
7	4.6594848473552764E-4	0.0	0.0	6.383261266634361	0.0
8	4.6594848473552764E-4	0.0	0.0	7.725658851157416	0.0
9	4.6594848473552764E-4	0.0	0.0	8.146876281358333	0.0
10	4.6594848473552764E-4	0.0	0.0	9.91305401274835	0.0
11	4.6594848473552764E-4	0.0	0.0	11.518246542662244	0.0
12	4.6594848473552764E-4	0.0	0.0	13.630856972453126	0.0
13	4.6594848473552764E-4	0.0	0.0	14.219582882916464	0.0
14	4.6594848473552764E-4	0.0	0.0	14.462342043463675	0.0
15	4.6594848473552764E-4	0.0	0.0	14.947860364558094	0.0
16	4.6594848473552764E-4	0.0	0.0	15.760707496179222	0.0
17	4.6594848473552764E-4	0.0	0.0	16.85778320348902	0.0
18	4.6594848473552764E-4	0.0	0.0	17.907565139598166	0.0
19	4.6594848473552764E-4	0.0	0.0	18.723673910612444	0.0
20	4.6594848473552764E-4	0.0	0.0	19.386252655906365	0.0
21	4.6594848473552764E-4	0.0	0.0	20.16694934208074	0.0
22	4.6594848473552764E-4	0.0	0.0	21.00658851157416	0.0
23	4.6594848473552764E-4	0.0	0.0	21.821765385618967	0.0
24	4.6594848473552764E-4	0.0	0.0	22.440777947590114	0.0
25	4.6594848473552764E-4	0.0	0.0	23.00410966563537	0.0
26	4.6594848473552764E-4	0.0	0.0	23.54041637156596	0.0
27	4.6594848473552764E-4	0.0	0.0	24.079285794162598	0.0
28	4.6594848473552764E-4	0.0	0.0	24.618621165243972	0.0
29	4.6594848473552764E-4	0.0	0.0	25.202221642375218	0.0
30	4.6594848473552764E-4	0.0	0.0	25.884603198270398	0.0
31	4.6594848473552764E-4	0.0	0.0	26.45748686025273	0.0
32	4.6594848473552764E-4	0.0	0.0	27.007073097998287	0.0
33	4.6594848473552764E-4	0.0	0.0	27.569472919074066	0.0
34	4.6594848473552764E-4	0.0	0.0	28.13047489469564	0.0
35	4.6594848473552764E-4	0.0	0.0	28.72572408394528	0.0
36	4.6594848473552764E-4	0.0	0.0	29.27367950199426	0.0
37	4.6594848473552764E-4	0.0	0.0	29.83957393670556	0.0
38	4.6594848473552764E-4	0.0	0.0	30.401740783538973	0.0
39	4.6594848473552764E-4	0.0	0.0	30.962043836433445	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	45	3.8380676E-10	45.000004	9
CGAACCC	40	6.7975634E-9	45.0	34
CGCGGGT	20	7.028342E-4	45.0	2
CCGATCG	20	7.028342E-4	45.0	40
GCGAACC	40	6.7975634E-9	45.0	33
TACGGGT	20	7.028342E-4	45.0	4
GTGCTAG	25	3.8867074E-5	45.0	1
CTACGCG	20	7.028342E-4	45.0	1
CGTAAGC	20	7.028342E-4	45.0	43
AGTACGG	40	6.7975634E-9	45.0	2
GTACGAG	25	3.8867074E-5	45.0	1
TCGTTGA	30	2.162311E-6	44.999996	24
CGTTGAT	30	2.162311E-6	44.999996	25
GTTAGCG	30	2.162311E-6	44.999996	1
TCGTGTA	30	2.162311E-6	44.999996	17
CGTGTAC	30	2.162311E-6	44.999996	18
TAGTACG	30	2.162311E-6	44.999996	1
GGCGAAC	105	0.0	42.857143	32
CGTTTTT	975	0.0	42.69231	1
TGCGGGT	85	0.0	42.35294	4
>>END_MODULE