##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935547.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742111
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.193714956387925	31.0	31.0	33.0	28.0	34.0
2	31.20309630230518	31.0	31.0	34.0	28.0	34.0
3	30.99404940770316	31.0	31.0	34.0	27.0	34.0
4	34.8073778720434	35.0	35.0	37.0	32.0	37.0
5	34.83088109460714	35.0	35.0	37.0	33.0	37.0
6	35.01373379454017	36.0	35.0	37.0	32.0	37.0
7	35.4306269547278	37.0	35.0	37.0	33.0	37.0
8	35.187445004857764	37.0	35.0	37.0	32.0	37.0
9	37.11771015387186	39.0	37.0	39.0	33.0	39.0
10	36.4514998430154	38.0	35.0	39.0	32.0	39.0
11	36.71041528827898	39.0	37.0	39.0	32.0	39.0
12	36.88979950438681	39.0	37.0	39.0	33.0	39.0
13	36.969162295128356	39.0	37.0	39.0	33.0	39.0
14	38.076475082568514	40.0	37.0	41.0	33.0	41.0
15	38.22007354694917	40.0	37.0	41.0	33.0	41.0
16	38.18442119844605	40.0	37.0	41.0	34.0	41.0
17	38.05084684097123	40.0	37.0	41.0	33.0	41.0
18	37.98985326992862	39.0	37.0	41.0	33.0	41.0
19	37.838495858436275	39.0	37.0	41.0	33.0	41.0
20	37.80197706273051	39.0	36.0	41.0	33.0	41.0
21	37.81297811243871	39.0	36.0	41.0	33.0	41.0
22	37.92215315498625	40.0	36.0	41.0	33.0	41.0
23	37.95164200503698	40.0	36.0	41.0	34.0	41.0
24	37.91023445279749	40.0	36.0	41.0	33.0	41.0
25	37.53073731557678	39.0	36.0	41.0	33.0	41.0
26	37.59397583380384	39.0	36.0	41.0	33.0	41.0
27	37.655217346192146	40.0	36.0	41.0	33.0	41.0
28	37.548779090998515	40.0	36.0	41.0	33.0	41.0
29	37.48791757567264	40.0	36.0	41.0	33.0	41.0
30	37.176807782124236	39.0	35.0	41.0	32.0	41.0
31	37.12878531648231	39.0	35.0	41.0	32.0	41.0
32	36.96601586555111	39.0	35.0	41.0	31.0	41.0
33	36.865024234919034	39.0	35.0	41.0	31.0	41.0
34	36.74112767497046	39.0	35.0	41.0	31.0	41.0
35	36.67073658792283	40.0	35.0	41.0	30.0	41.0
36	36.55158325371811	39.0	35.0	41.0	30.0	41.0
37	36.504145606250276	39.0	35.0	41.0	30.0	41.0
38	36.387648208960655	39.0	35.0	41.0	30.0	41.0
39	36.27873323532464	39.0	35.0	41.0	30.0	41.0
40	36.22081737098628	39.0	35.0	41.0	30.0	41.0
41	36.103279698050564	39.0	35.0	41.0	29.0	41.0
42	36.1417186916782	39.0	35.0	41.0	29.0	41.0
43	36.129707011484804	39.0	35.0	41.0	29.0	41.0
44	36.111192260996	39.0	35.0	41.0	29.0	41.0
45	36.081041784854285	39.0	35.0	41.0	29.0	41.0
46	35.95502963842336	39.0	35.0	41.0	28.0	41.0
47	35.90090026963621	39.0	35.0	41.0	28.0	41.0
48	35.83539389660037	39.0	35.0	40.0	28.0	41.0
49	35.82369079558179	39.0	35.0	40.0	28.0	41.0
50	35.735790198501306	39.0	35.0	40.0	28.0	41.0
51	34.616030486005464	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	24.0
9	48.0
10	53.0
11	37.0
12	35.0
13	28.0
14	36.0
15	44.0
16	83.0
17	163.0
18	325.0
19	521.0
20	986.0
21	1666.0
22	2510.0
23	3380.0
24	4816.0
25	6981.0
26	9119.0
27	9789.0
28	10074.0
29	10365.0
30	12025.0
31	15146.0
32	19229.0
33	27291.0
34	45229.0
35	52985.0
36	60211.0
37	92469.0
38	165356.0
39	191053.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.63620940802656	19.96561161335703	22.403521845114817	14.994657133501594
2	35.18732372919954	24.937239846869268	23.38491142160674	16.49052500232445
3	30.452048278492033	24.52436360598347	29.14267542187085	15.880912693653645
4	27.62524743603046	23.50537857544222	30.319184057371473	18.550189931155852
5	25.519497757074078	28.363546693149676	26.76553776995625	19.35141777982
6	23.6708524735518	34.72485921917341	27.080584979874978	14.52370332739981
7	75.45569328577531	5.507127639935265	14.292875324580825	4.744303749708602
8	76.06314958274436	6.880507093952253	11.996318610019257	5.060024713284132
9	69.84076506075236	7.533374387389488	13.897381928040414	8.72847862381773
10	37.338214903161386	26.928586154901357	20.984461893166927	14.748737048770334
11	27.163187178198413	24.711936624036028	28.86077689186658	19.26409930589898
12	24.7080288528266	21.509585493275267	32.94520631010725	20.837179343790886
13	23.210543975227427	22.61804500943929	34.852872413965024	19.31853860136826
14	19.426878189381373	27.696395822188325	32.01946878566683	20.857257202763467
15	18.515154741002355	26.42273190937744	35.693986479111615	19.36812687050859
16	21.193595028237016	24.93373632785392	33.02228372844493	20.85038491546413
17	20.519437119244966	26.026969011374305	31.441657649596895	22.011936219783834
18	21.509046490349824	26.1687267807646	30.560657367967863	21.761569360917708
19	22.025276542188433	27.612715618014015	29.09066163956605	21.271346200231502
20	24.242330325247842	26.192442909483894	30.510530095902094	19.054696669366173
21	23.0522118658799	27.307505211484536	30.213269982522828	19.427012940112732
22	21.243452798840064	24.09949455000667	31.062469091550994	23.59458355960227
23	22.00883695296256	26.170882792466355	30.865194020840548	20.955086233730533
24	22.044545896772856	25.149067996566547	30.625337719020468	22.181048387640125
25	21.317565701087844	27.770778225898823	28.766720881377584	22.144935191635753
26	20.68733653051902	26.70260917841132	30.05022159757772	22.559832693491945
27	22.739859670588363	26.707190703277544	29.237000933822564	21.31594869231153
28	20.614705886316198	27.406142746839755	30.57062892208847	21.40852244475557
29	23.398386494742702	25.114841310801218	29.856315295151266	21.63045689930482
30	22.521832987248537	26.60504964890697	30.253695201930707	20.619422161913782
31	24.112565370948552	26.01403294116379	27.560432334246492	22.312969353641165
32	24.333960822572365	26.895437474986895	29.018030995363226	19.752570707077513
33	21.980943551571126	26.021444231388568	29.6320900781689	22.36552213887141
34	22.008163199305763	25.61234101098084	29.40840386411197	22.971091925601424
35	21.416472737905785	26.919557855900262	28.513659007884268	23.15031039830969
36	22.078368330344116	28.15683907124406	27.242151106775133	22.522641491636698
37	21.390735348216104	27.879117813911936	29.169086565217334	21.56106027265463
38	21.146162770798437	27.393341427360596	28.370553731180376	23.089942070660587
39	22.112190763915372	24.83038251690111	29.161136272067118	23.896290447116403
40	21.813717893953868	25.160791310194835	31.184822755625508	21.84066804022579
41	19.34858801446145	26.933167679767582	28.861720146986098	24.85652415878487
42	21.35448740148037	27.43996518041102	28.707969562504797	22.49757785560381
43	22.439500290387826	25.549008167241826	28.52147455030312	23.490016992067225
44	21.92960352292312	25.29217327327044	28.77264991355741	24.005573290249032
45	21.635577427096486	24.340024605483546	28.868457683554077	25.155940283865892
46	23.161359958281174	26.064968717617713	27.77023922297338	23.00343210112773
47	20.94996570593887	25.534455088254994	31.259339910067364	22.256239295738776
48	20.76697421275254	25.235712716830772	29.541537586695256	24.455775483721435
49	20.399913220529005	24.57489513024332	31.410530230652828	23.61466141857485
50	19.80714475327815	23.924857602164636	31.87097347970856	24.397024164848656
51	19.825470852743056	23.423450130775585	29.92113039693523	26.829948619546133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	283.0
1	602.0
2	921.0
3	6241.0
4	11561.0
5	7712.5
6	3864.0
7	3770.5
8	3677.0
9	3604.0
10	3531.0
11	3453.5
12	3376.0
13	3212.5
14	3049.0
15	2920.0
16	2791.0
17	2762.0
18	2733.0
19	2707.0
20	2681.0
21	2781.5
22	2882.0
23	3121.0
24	3360.0
25	4022.0
26	5542.5
27	6401.0
28	7640.5
29	8880.0
30	10518.0
31	12156.0
32	14179.5
33	16203.0
34	17959.0
35	19715.0
36	21368.5
37	23022.0
38	25088.0
39	27154.0
40	30199.0
41	33244.0
42	36335.0
43	39426.0
44	43644.5
45	47863.0
46	63751.5
47	79640.0
48	74299.0
49	68958.0
50	67777.0
51	66596.0
52	58024.0
53	49452.0
54	45113.5
55	40775.0
56	37156.0
57	33537.0
58	31571.0
59	29605.0
60	28795.0
61	27985.0
62	24756.0
63	21527.0
64	18361.0
65	15195.0
66	12439.0
67	9683.0
68	7933.5
69	6184.0
70	4981.5
71	3779.0
72	3136.0
73	2493.0
74	2126.5
75	1200.0
76	640.0
77	579.0
78	518.0
79	349.0
80	180.0
81	125.0
82	70.0
83	58.0
84	46.0
85	34.0
86	22.0
87	12.0
88	2.0
89	3.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	742111.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.642184993400775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.11297896553114	26.05906321708081
2	9.029909831408368	6.436914333695711
3	3.467645554102633	3.7078339299260943
4	2.1223832587055727	3.0258550693472324
5	1.523370026877516	2.7148118155685173
6	1.2396445026915128	2.6510183214589924
7	1.0404117471645242	2.59577835732217
8	0.923934088672829	2.63448237681489
9	0.8517207055557443	2.7321468255114905
>10	6.597858102288509	38.96071735273899
>50	0.054620678681372416	1.3476486984183302
>100	0.02826524554515397	1.913173730989439
>500	0.0030557022210977143	0.8083986063200217
>1k	0.003437664998734928	2.568551112401457
>5k	7.639255552744286E-4	1.8436062524058157
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6994	0.9424466151290035	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	6548	0.8823477889426244	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	4072	0.5487049780962686	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG	3626	0.4886061519098895	No Hit
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	2455	0.3308130454878044	TruSeq Adapter, Index 19 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	1852	0.24955835447796892	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTT	1780	0.23985630182007814	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1527	0.20576436678610074	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	1392	0.18757301805255547	No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	1119	0.1507860683913862	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT	1044	0.1406797635394166	No Hit
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	877	0.11817639140236434	TruSeq Adapter, Index 13 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	871	0.11736788701420676	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT	864	0.11642463189468961	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC	744	0.10025454413153828	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0425219407878334E-4	0.0	0.0	0.12262316553723095	0.0
2	4.0425219407878334E-4	0.0	0.0	0.7148526298626485	0.0
3	4.0425219407878334E-4	0.0	0.0	1.0939064371771878	0.0
4	4.0425219407878334E-4	0.0	0.0	1.7465042291517037	0.0
5	4.0425219407878334E-4	0.0	0.0	3.6789644675796476	0.0
6	4.0425219407878334E-4	0.0	0.0	4.849948323094524	0.0
7	4.0425219407878334E-4	0.0	0.0	5.731083355454912	0.0
8	4.0425219407878334E-4	0.0	0.0	6.912982020209915	0.0
9	4.0425219407878334E-4	0.0	0.0	7.394985386283184	0.0
10	4.0425219407878334E-4	0.0	0.0	8.736294166236588	0.0
11	4.0425219407878334E-4	0.0	0.0	9.81847728978549	0.0
12	4.0425219407878334E-4	0.0	0.0	11.220423898850711	0.0
13	4.0425219407878334E-4	0.0	0.0	11.66254104844154	0.0
14	4.0425219407878334E-4	0.0	0.0	11.874638699601542	0.0
15	4.0425219407878334E-4	0.0	0.0	12.234962155257097	0.0
16	4.0425219407878334E-4	0.0	0.0	12.785014640666962	0.0
17	4.0425219407878334E-4	0.0	0.0	13.434513165820208	0.0
18	5.390029254383779E-4	0.0	0.0	14.144245267891192	0.0
19	5.390029254383779E-4	0.0	0.0	14.687155964538997	0.0
20	5.390029254383779E-4	0.0	0.0	15.12873411120439	0.0
21	5.390029254383779E-4	0.0	0.0	15.64685067328203	0.0
22	6.737536567979723E-4	0.0	0.0	16.240158143458324	0.0
23	6.737536567979723E-4	0.0	0.0	16.831444352664224	0.0
24	6.737536567979723E-4	0.0	0.0	17.303206663154164	0.0
25	6.737536567979723E-4	0.0	0.0	17.725515455235133	0.0
26	6.737536567979723E-4	0.0	0.0	18.09500196062314	0.0
27	6.737536567979723E-4	0.0	0.0	18.509090958091175	0.0
28	6.737536567979723E-4	0.0	0.0	18.90660561560198	0.0
29	6.737536567979723E-4	0.0	0.0	19.332148425235577	0.0
30	6.737536567979723E-4	0.0	0.0	19.831804137116954	0.0
31	8.085043881575667E-4	0.0	0.0	20.280524072544402	0.0
32	8.085043881575667E-4	0.0	0.0	20.74312333330189	0.0
33	8.085043881575667E-4	0.0	0.0	21.170148400980445	0.0
34	8.085043881575667E-4	0.0	0.0	21.589762178434224	0.0
35	8.085043881575667E-4	0.0	0.0	22.03619135142856	0.0
36	8.085043881575667E-4	0.0	0.0	22.48410278246785	0.0
37	8.085043881575667E-4	0.0	0.0	22.939156002269204	0.0
38	8.085043881575667E-4	0.0	0.0	23.394882975727352	0.0
39	8.085043881575667E-4	0.0	0.0	23.831475345332436	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTCGT	20	7.03167E-4	45.0	15
CGTTTTT	3640	0.0	43.02198	1
CTCGAAT	85	0.0	42.35294	43
TAGTGCG	60	3.6379788E-12	41.250004	1
TACGGCT	520	0.0	41.105766	7
GCGTTAG	50	1.0804797E-9	40.5	1
GCCGATT	45	1.9266736E-8	40.0	9
TGTTGCG	85	0.0	39.705883	1
GTAAGCG	40	3.456007E-7	39.375	1
AAATCGG	35	6.2452964E-6	38.571426	2
CGATAAG	35	6.2452964E-6	38.571426	10
CGATGAA	535	0.0	38.271027	19
CGTCTCG	30	1.1395318E-4	37.500004	41
AATAGCG	30	1.1395318E-4	37.500004	1
TCGAATG	85	0.0	37.058823	44
GCGAACC	55	2.746674E-9	36.81818	33
CACGACG	55	2.746674E-9	36.81818	26
GATGAAT	575	0.0	36.782608	20
CGAGATA	245	0.0	36.734695	19
CGCATGG	80	0.0	36.5625	2
>>END_MODULE