##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935546.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468594
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.122545316414637	31.0	31.0	33.0	28.0	34.0
2	31.166199311130743	31.0	31.0	34.0	28.0	34.0
3	30.92541731221484	31.0	30.0	34.0	27.0	34.0
4	34.72025036598847	35.0	35.0	37.0	32.0	37.0
5	34.763708028698616	35.0	35.0	37.0	32.0	37.0
6	34.94760496293166	36.0	35.0	37.0	32.0	37.0
7	35.35569810966423	37.0	35.0	37.0	33.0	37.0
8	35.17656222657567	37.0	35.0	37.0	32.0	37.0
9	37.10824509063283	39.0	37.0	39.0	34.0	39.0
10	36.43790146694153	38.0	35.0	39.0	32.0	39.0
11	36.64065480992074	39.0	35.0	39.0	32.0	39.0
12	36.79601317985292	39.0	35.0	39.0	33.0	39.0
13	36.84061682394567	39.0	37.0	39.0	33.0	39.0
14	37.84696346944263	40.0	37.0	41.0	33.0	41.0
15	38.03305206639436	40.0	37.0	41.0	33.0	41.0
16	38.026560305936485	40.0	37.0	41.0	33.0	41.0
17	37.911053065126744	40.0	37.0	41.0	33.0	41.0
18	37.82302376897698	39.0	37.0	41.0	33.0	41.0
19	37.63254971254433	39.0	37.0	40.0	33.0	41.0
20	37.5781806851987	39.0	35.0	41.0	33.0	41.0
21	37.5454935402502	39.0	35.0	41.0	33.0	41.0
22	37.65820945210566	39.0	35.0	41.0	33.0	41.0
23	37.71990038284741	39.0	35.0	41.0	33.0	41.0
24	37.67384985723249	39.0	35.0	41.0	33.0	41.0
25	37.268836135332506	39.0	35.0	41.0	32.0	41.0
26	37.318535875406	39.0	35.0	41.0	33.0	41.0
27	37.3738801606508	39.0	35.0	41.0	33.0	41.0
28	37.240993269226664	39.0	35.0	41.0	33.0	41.0
29	37.21289004980858	39.0	35.0	41.0	32.0	41.0
30	36.88635364515977	39.0	35.0	41.0	31.0	41.0
31	36.82351886707897	39.0	35.0	41.0	31.0	41.0
32	36.66817970353867	39.0	35.0	41.0	31.0	41.0
33	36.51546114546921	39.0	35.0	41.0	30.0	41.0
34	36.31658322556414	39.0	35.0	41.0	30.0	41.0
35	36.250073624502235	39.0	35.0	41.0	30.0	41.0
36	36.08431606038489	39.0	35.0	41.0	29.0	41.0
37	36.07775387649009	39.0	35.0	41.0	29.0	41.0
38	35.92360764329035	39.0	35.0	41.0	28.0	41.0
39	35.82484837620627	39.0	35.0	41.0	27.0	41.0
40	35.725280306619375	39.0	35.0	41.0	27.0	41.0
41	35.566138277485415	39.0	35.0	40.0	26.0	41.0
42	35.60883195260716	39.0	35.0	41.0	26.0	41.0
43	35.584638300959895	39.0	35.0	40.0	26.0	41.0
44	35.56421977234024	39.0	35.0	40.0	26.0	41.0
45	35.55378429941484	39.0	35.0	40.0	26.0	41.0
46	35.45391746373193	38.0	35.0	40.0	26.0	41.0
47	35.41504159250865	38.0	35.0	40.0	26.0	41.0
48	35.34740308241249	38.0	35.0	40.0	26.0	41.0
49	35.33905257002864	38.0	35.0	40.0	26.0	41.0
50	35.242467893314895	38.0	35.0	40.0	26.0	41.0
51	34.146922495806606	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	20.0
9	22.0
10	34.0
11	19.0
12	18.0
13	21.0
14	31.0
15	42.0
16	66.0
17	121.0
18	222.0
19	368.0
20	663.0
21	1090.0
22	1654.0
23	2445.0
24	3791.0
25	5401.0
26	6959.0
27	7605.0
28	7573.0
29	7647.0
30	8468.0
31	10381.0
32	13540.0
33	18606.0
34	30569.0
35	33975.0
36	38856.0
37	58099.0
38	99269.0
39	111011.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4384307097402	19.469519456074984	22.204936469523723	16.887113364661094
2	37.006235675232716	23.746142716295985	22.975966401618457	16.27165520685284
3	29.938710269444336	23.95527898351238	30.36658599982074	15.739424747222541
4	26.611736385869218	23.501154517556778	31.483117581531133	18.403991515042872
5	24.866515576383822	27.86954165012783	28.594689646047538	18.669253127440815
6	23.572858380602398	32.8941471721789	28.785259734439624	14.747734712779078
7	74.03018391187254	5.4772361575265585	15.447487590536799	5.045092340064106
8	75.27155704084986	5.904471674840053	13.881526438665453	4.942444845644631
9	69.5333273580114	6.714768008126438	15.544586571744409	8.20731806211774
10	37.21750598599214	26.077371882695893	22.365416543959164	14.339705587352805
11	27.006747845683044	24.05493881697162	30.424631984191002	18.51368135315433
12	23.745502503233077	21.27065220638762	34.53287920886738	20.450966081511925
13	23.773671878001	21.456100590276446	36.644301890335775	18.125925641386786
14	19.825051110342855	25.83003623605936	34.53095856967865	19.81395408391913
15	18.994908172106342	24.932457521863277	36.7520710892585	19.320563216771873
16	22.631958582482916	25.057299069130206	32.88240993269227	19.42833241569461
17	22.830851440692797	25.63711869976995	31.365531782310484	20.16649807722677
18	22.857526984980602	24.52955010094026	32.214667708079915	20.398255205999224
19	22.16524325962347	26.37528436130211	30.389633670085402	21.069838708989018
20	23.15159818520937	26.46299355092041	31.148712958339203	19.236695305531015
21	22.723722454832966	26.550489336184413	32.65257344310854	18.07321476587408
22	21.617434282129093	23.115319444977956	33.32052907207519	21.946717200817766
23	21.070692326406228	25.17467146399655	32.93255995595334	20.82207625364388
24	21.646670678668528	25.095285044196043	31.772707290319552	21.485336986815877
25	22.24804414909282	26.394063944480724	29.96773326162947	21.39015864479699
26	20.76723133458815	25.05409800381567	30.96369138315898	23.214979278437198
27	21.860928650388185	25.02955650307089	31.339283046731286	21.770231799809643
28	19.517535435793032	25.875277959171477	31.766518564044784	22.840668040990707
29	20.96783142763245	25.219486378400063	30.90671242056023	22.905969773407257
30	21.108251492763458	27.008881889226068	30.842050901206587	21.040815716803884
31	21.596734059761754	25.377405600583874	30.319850446228504	22.706009893425865
32	21.766817330140803	27.594036628723373	28.91671681668993	21.722429224445897
33	20.98255632807932	27.135003862618813	28.982445357815077	22.899994451486787
34	19.769992786932825	26.049842720990878	29.9444721870105	24.235692305065793
35	19.624237612944253	27.540045327084854	28.26903460138201	24.566682458588886
36	20.513280152968242	27.601505781123958	27.726133924036585	24.159080141871215
37	19.791973435425977	28.03727747260955	28.54091174876332	23.629837343201153
38	19.800723013952375	28.10599367469494	27.96130552247788	24.131977788874803
39	20.872439681259255	26.153557237181868	27.559038314617773	25.414964766941107
40	21.302662859532987	25.08141376116638	29.642931834381148	23.972991544919484
41	19.58198355079237	25.510783322022903	28.77032142963845	26.136911697546278
42	20.211739800338886	25.748302368361525	29.715702719198283	24.324255112101305
43	21.199161747696298	24.883161116019412	29.586593084845305	24.331084051438985
44	21.28473689377158	25.187902533963303	29.557783497014473	23.96957707525064
45	21.093739996670894	24.52955010094026	28.877237011143976	25.499472891244874
46	21.8536729023419	25.754917903344897	28.318117602871567	24.07329159144163
47	19.616981864897973	25.34987643887886	31.668139156711355	23.365002539511817
48	19.79730854428354	25.45124350717252	29.9094738729049	24.84197407563904
49	20.6929666192909	24.527629461751538	31.212734264629937	23.566669654327626
50	19.51113330516396	24.34346150398853	31.69289406181044	24.452511129037077
51	19.53930267993188	23.771537834457973	29.87789002846814	26.811269457142007
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	221.0
1	405.5
2	590.0
3	5217.0
4	9844.0
5	6448.5
6	3053.0
7	2989.5
8	2926.0
9	2892.0
10	2858.0
11	2793.0
12	2728.0
13	2626.0
14	2524.0
15	2444.0
16	2364.0
17	2214.5
18	2065.0
19	1911.0
20	1757.0
21	1922.0
22	2087.0
23	2265.0
24	2443.0
25	2640.5
26	3395.0
27	3952.0
28	4458.0
29	4964.0
30	5922.0
31	6880.0
32	7837.5
33	8795.0
34	10050.0
35	11305.0
36	12545.5
37	13786.0
38	15248.5
39	16711.0
40	18606.5
41	20502.0
42	22041.5
43	23581.0
44	26385.0
45	29189.0
46	38590.0
47	47991.0
48	45999.5
49	44008.0
50	43134.5
51	42261.0
52	37382.5
53	32504.0
54	29358.5
55	26213.0
56	24403.0
57	22593.0
58	20940.5
59	19288.0
60	17526.0
61	15764.0
62	14338.0
63	12912.0
64	11190.5
65	9469.0
66	7935.0
67	6401.0
68	5476.5
69	4552.0
70	3672.0
71	2792.0
72	2204.5
73	1617.0
74	1301.5
75	825.5
76	665.0
77	493.0
78	321.0
79	225.5
80	130.0
81	110.0
82	90.0
83	66.5
84	43.0
85	28.5
86	14.0
87	10.5
88	7.0
89	5.5
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	468594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.04565210317632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.3350021692607	29.690373339219793
2	9.09431004702971	7.465637726176051
3	4.241181210423192	5.222461454086757
4	2.6408211209440426	4.335768999879572
5	2.0884847705421805	4.286160965722819
6	1.624818242266877	4.001503458178843
7	1.3524660222307943	3.8858994880896343
8	1.13119730869902	3.714458495432746
9	0.9199859914884824	3.398528245178878
>10	4.515988719659905	26.826123599579994
>50	0.03050165143769784	0.8485268003389036
>100	0.01788027842899003	1.457670181299241
>500	0.003155343252174711	0.8841429087222997
>1k	0.0036812337942038293	2.6174601833902846
>5k	5.258905420291185E-4	1.365284154704235
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6325	1.3497825409629658	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC	3885	0.8290759164649995	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG	1704	0.3636410197313666	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC	1666	0.3555316542678737	No Hit
GAACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT	1314	0.280413321553413	No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	1290	0.27529161705015426	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT	1211	0.2584326730602611	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC	1056	0.2253549981433821	No Hit
CGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTG	997	0.21276414123953788	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT	770	0.1643213528128828	No Hit
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	618	0.13188389095891112	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT	610	0.13017665612449156	No Hit
CGTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT	581	0.12398792984972065	No Hit
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT	520	0.1109702642372715	TruSeq Adapter, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1340435430244518E-4	0.0	0.0	0.11694558615773996	0.0
2	4.2680870860489035E-4	0.0	0.0	0.6278356103577938	0.0
3	4.2680870860489035E-4	0.0	0.0	1.020072813565688	0.0
4	4.2680870860489035E-4	0.0	0.0	1.6880284425323415	0.0
5	4.2680870860489035E-4	0.0	0.0	3.512208863109643	0.0
6	4.2680870860489035E-4	0.0	0.0	4.777269875414538	0.0
7	6.402130629073355E-4	0.0	0.0	5.7565824573084585	0.0
8	6.402130629073355E-4	0.0	0.0	7.01609495640149	0.0
9	6.402130629073355E-4	0.0	0.0	7.662069936874992	0.0
10	8.536174172097807E-4	0.0	0.0	8.91518030533895	0.0
11	8.536174172097807E-4	0.0	0.0	9.939948014699292	0.0
12	8.536174172097807E-4	0.0	0.0	11.064162153164574	0.0
13	8.536174172097807E-4	0.0	0.0	11.490330648706557	0.0
14	8.536174172097807E-4	0.0	0.0	11.719953733935988	0.0
15	8.536174172097807E-4	0.0	0.0	12.04496856553861	0.0
16	8.536174172097807E-4	0.0	0.0	12.489489835550605	0.0
17	8.536174172097807E-4	0.0	0.0	13.030256469353	0.0
18	0.0010670217715122259	0.0	0.0	13.61583801755891	0.0
19	0.0010670217715122259	0.0	0.0	14.063773757239742	0.0
20	0.0010670217715122259	0.0	0.0	14.442779890480885	0.0
21	0.0010670217715122259	0.0	0.0	14.869802003440078	0.0
22	0.0010670217715122259	0.0	0.0	15.34654733095174	0.0
23	0.0010670217715122259	0.0	0.0	15.767594121990465	0.0
24	0.0010670217715122259	0.0	0.0	16.14425280733428	0.0
25	0.0010670217715122259	0.0	0.0	16.473535726022952	0.0
26	0.0010670217715122259	0.0	0.0	16.8004711968143	0.0
27	0.0010670217715122259	0.0	0.0	17.16795349492311	0.0
28	0.0010670217715122259	0.0	0.0	17.506839609555392	0.0
29	0.0010670217715122259	0.0	0.0	17.879870420876067	0.0
30	0.0010670217715122259	0.0	0.0	18.335061908603183	0.0
31	0.0010670217715122259	0.0	0.0	18.727299111811078	0.0
32	0.0010670217715122259	0.0	0.0	19.11057333213827	0.0
33	0.0010670217715122259	0.0	0.0	19.500036278740232	0.0
34	0.001280426125814671	0.0	0.0	19.877335177146954	0.0
35	0.001280426125814671	0.0	0.0	20.28963238965928	0.0
36	0.0017072348344195614	0.0	0.0	20.666504479357396	0.0
37	0.0017072348344195614	0.0	0.0	21.040815716803884	0.0
38	0.0019206391887220066	0.0	0.0	21.4785080474782	0.0
39	0.0019206391887220066	0.0	0.0	22.095033227057964	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	50	2.1827873E-11	45.0	25
TTAATCG	20	7.029004E-4	45.0	20
AACGGGC	45	3.8380676E-10	45.0	4
ACGGGCC	30	2.1627402E-6	44.999996	5
CGTTTTT	3125	0.0	43.704	1
CGGCGAA	65	0.0	41.538464	31
CGGTCTA	50	1.0786607E-9	40.5	31
GTGCACG	40	3.452369E-7	39.375	1
TTGTGCG	40	3.452369E-7	39.375	1
TACGGGA	145	0.0	38.793106	4
CCATACG	65	9.094947E-12	38.076927	2
CTATGTA	30	1.1388902E-4	37.499996	30
TGCGCAG	60	1.546141E-10	37.499996	1
CGGGCGA	120	0.0	37.499996	6
TTTGACG	30	1.1388902E-4	37.499996	1
GTAGTAT	30	1.1388902E-4	37.499996	45
ATTACGG	55	2.741217E-9	36.818184	2
ACGGTCT	55	2.741217E-9	36.818184	30
GTTTTTT	3810	0.0	36.377956	2
TTACGGG	155	0.0	36.29032	3
>>END_MODULE