SRR2935543.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935543.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	327137
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	2004	0.6125873869357487	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	1864	0.5697918609023132	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	1805	0.5517566035025081	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1140	0.34847785484368937	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	756	0.23109584058055185	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	723	0.2210083237298135	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	423	0.12930362508673734	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	421	0.12869226042911686	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	398	0.12166156686648102	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	364	0.11126836768693238	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	349	0.10668313275477859	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	45	3.8380676E-10	45.000004	25
CTCACGA	45	3.8380676E-10	45.000004	24
CCTTCGT	20	7.025879E-4	45.0	33
TTGTGCG	35	1.2083729E-7	45.0	1
GACGTAG	20	7.025879E-4	45.0	1
TGCGGTC	20	7.025879E-4	45.0	15
CCCGTAA	20	7.025879E-4	45.0	43
CCGTAAC	20	7.025879E-4	45.0	44
TCTAAAC	20	7.025879E-4	45.0	34
GCGATAT	20	7.025879E-4	45.0	9
CCCCACG	20	7.025879E-4	45.0	38
CGTAACG	20	7.025879E-4	45.0	45
ACCCGTA	20	7.025879E-4	45.0	42
CAATCAC	20	7.025879E-4	45.0	42
ATCCGGT	25	3.884668E-5	44.999996	11
CGTAAGG	25	3.884668E-5	44.999996	2
TATCCGG	25	3.884668E-5	44.999996	10
TAGGGTA	125	0.0	43.2	5
TAACGGG	75	0.0	42.0	3
CACGGGC	65	0.0	41.538464	4