##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935543.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	327137
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.253609344097427	31.0	31.0	33.0	28.0	34.0
2	31.255694097579912	31.0	31.0	34.0	28.0	34.0
3	31.082185750923927	31.0	31.0	34.0	28.0	34.0
4	34.92840002812277	35.0	35.0	37.0	32.0	37.0
5	34.90563892191956	35.0	35.0	37.0	33.0	37.0
6	35.07947129184409	37.0	35.0	37.0	32.0	37.0
7	35.54635519675243	37.0	35.0	37.0	33.0	37.0
8	35.15449490580399	37.0	35.0	37.0	32.0	37.0
9	37.038399814145144	39.0	37.0	39.0	33.0	39.0
10	36.506588982597506	38.0	35.0	39.0	32.0	39.0
11	36.72517630228314	39.0	35.0	39.0	32.0	39.0
12	36.82726808645919	39.0	35.0	39.0	33.0	39.0
13	36.84484482036578	39.0	37.0	39.0	33.0	39.0
14	37.91646007635945	40.0	37.0	41.0	33.0	41.0
15	38.083524639524114	40.0	37.0	41.0	33.0	41.0
16	38.06575838257366	40.0	37.0	41.0	34.0	41.0
17	37.94782919694195	40.0	37.0	41.0	33.0	41.0
18	37.92722315115685	39.0	37.0	41.0	33.0	41.0
19	37.82302215891202	39.0	37.0	41.0	33.0	41.0
20	37.852581640107964	39.0	36.0	41.0	33.0	41.0
21	37.836310169745396	40.0	36.0	41.0	33.0	41.0
22	37.929060301953	40.0	36.0	41.0	33.0	41.0
23	37.978611407453144	40.0	36.0	41.0	34.0	41.0
24	37.96096742343422	40.0	36.0	41.0	33.0	41.0
25	37.59594909777861	39.0	36.0	41.0	33.0	41.0
26	37.639190920012105	40.0	36.0	41.0	33.0	41.0
27	37.67404481914305	40.0	36.0	41.0	33.0	41.0
28	37.57153425017653	40.0	36.0	41.0	33.0	41.0
29	37.585369432378485	40.0	36.0	41.0	33.0	41.0
30	37.328846935687494	39.0	36.0	41.0	32.0	41.0
31	37.32102146806995	39.0	35.0	41.0	33.0	41.0
32	37.27980631967647	39.0	35.0	41.0	33.0	41.0
33	37.27920106866542	40.0	35.0	41.0	33.0	41.0
34	37.196694351296244	40.0	35.0	41.0	32.0	41.0
35	37.214900790800186	40.0	35.0	41.0	32.0	41.0
36	37.07874376790152	40.0	35.0	41.0	32.0	41.0
37	37.07053619737297	40.0	35.0	41.0	32.0	41.0
38	36.91060014611615	39.0	35.0	41.0	31.0	41.0
39	36.840421597067895	39.0	35.0	41.0	31.0	41.0
40	36.71896483736172	39.0	35.0	41.0	31.0	41.0
41	36.58509737510584	39.0	35.0	41.0	31.0	41.0
42	36.6148861180484	39.0	35.0	41.0	31.0	41.0
43	36.60236536986034	39.0	35.0	41.0	31.0	41.0
44	36.60975676857097	39.0	35.0	41.0	31.0	41.0
45	36.61765254312414	39.0	35.0	41.0	31.0	41.0
46	36.488348918037396	39.0	35.0	41.0	31.0	41.0
47	36.43282172300901	39.0	35.0	41.0	31.0	41.0
48	36.36292440170326	39.0	35.0	41.0	31.0	41.0
49	36.318083860890084	39.0	35.0	40.0	31.0	41.0
50	36.21246144581628	38.0	35.0	40.0	31.0	41.0
51	35.06218189932658	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	27.0
10	25.0
11	22.0
12	12.0
13	12.0
14	17.0
15	18.0
16	43.0
17	61.0
18	125.0
19	202.0
20	430.0
21	716.0
22	1047.0
23	1259.0
24	1680.0
25	2185.0
26	2608.0
27	2879.0
28	3320.0
29	3747.0
30	4833.0
31	6289.0
32	8481.0
33	12479.0
34	22847.0
35	24990.0
36	26440.0
37	40645.0
38	71300.0
39	88375.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.526990221222306	20.44220005685691	21.89571953034967	11.135090191571114
2	32.38795978443282	26.531086364428358	23.661340661557695	17.419613189581124
3	32.805827527916435	25.81945790295809	24.980665592702753	16.394048976422724
4	30.193466345904007	24.382445275221084	26.597725112108993	18.82636326676591
5	27.214897733976894	29.598914216368065	22.56883201838985	20.61735603126519
6	25.03354863558693	37.26787248155971	23.017573677083302	14.68100520577006
7	77.42138614708823	6.535793872292037	11.318193906528458	4.724626074091283
8	76.8424238163216	8.811598810284377	9.120949327040353	5.2250280463536685
9	70.61628614311435	8.9225614956425	10.85263971975044	9.608512641492709
10	41.42759761200965	26.094571998887318	17.732326211954014	14.745504177149025
11	31.28689203605829	26.101908374778766	23.30399801917851	19.307201569984443
12	28.97807340655444	21.852924004316236	27.970544450795842	21.198458138333482
13	25.62076438923142	24.02693672681476	29.51760271690454	20.83469616704928
14	20.12918135215521	29.83031573927743	27.29590355111161	22.744599357455744
15	19.110647832559447	26.877424442970376	32.917095895603374	21.094831828866806
16	21.93209572747809	25.114248770392834	30.37657006086135	22.577085441267723
17	21.780171610059394	25.79500331665327	28.54706132293198	23.877763750355356
18	22.043669777493832	26.454971464554607	28.308017741802367	23.193341016149198
19	24.440524917695033	27.020178090524766	25.67823266704775	22.861064324732453
20	27.482981136343486	25.24599785411005	28.095568523279233	19.175452486267222
21	25.44866523811125	27.39769576660543	26.52008180059119	20.63355719469213
22	22.35424302356505	25.129227204504534	27.23171026206146	25.284819509868957
23	23.544570011952178	26.760959475693667	27.75259295035413	21.941877562000016
24	23.81112500267472	24.958962147357223	27.514466416210947	23.715446433757112
25	22.47131935549938	28.077227583550624	25.942647881468623	23.508805179481378
26	21.928427539532365	26.894848335712563	27.909713667362603	23.267010457392466
27	24.768216374179623	26.82301298844215	27.04004744189743	21.368723195480793
28	20.54582636632359	27.90543411475926	29.232706786453384	22.31603273246377
29	23.200371709711835	26.08448448203658	28.910517611887375	21.804626196364215
30	23.74846012526862	26.889957418451598	27.44354811592697	21.91803434035282
31	24.692101474305872	26.109244750670207	25.603951861146857	23.59470191387706
32	26.211648330821642	28.212033490555942	25.35390371618007	20.22241446244234
33	24.971189440509633	27.476561807438475	24.603453598950896	22.948795153100995
34	23.793701109932535	26.502657907849002	26.989304175314928	22.71433680690353
35	24.561880802232704	26.315274640288322	25.566047252374386	23.556797305104592
36	23.391423165218242	28.389023558937083	26.130031149029303	22.08952212681537
37	25.0149020135295	27.767571384465832	26.473006721954413	20.744519880050255
38	22.61865823798592	27.958011475314628	25.921250118451905	23.502080168247556
39	24.866646084056526	25.633297364712643	26.169158487117016	23.33089806411381
40	23.724616903621417	26.217761977397846	29.082005398349924	20.975615720630806
41	22.013101544612805	26.671700235681072	26.928779074210503	24.38641914549562
42	21.966332148304836	27.201447711509246	27.723553129117157	23.108667011068757
43	23.970691178313672	25.141148815328133	26.81139705994736	24.07676294641083
44	23.313779853700435	26.15173459437483	27.347258182351737	23.187227369572994
45	23.296661643287063	24.682319639783945	26.468115804693447	25.55290291223555
46	23.621907641141174	27.4236787645543	25.980858172569903	22.973555421734627
47	22.76324597951317	25.778190788568704	29.52983001005695	21.928733221861176
48	23.010848665849476	24.950403042150537	27.91429890229476	24.12444938970523
49	21.845587628424788	24.879790424195367	29.827258915989326	23.44736303139052
50	21.128762567364742	25.140537450670514	29.39594115003806	24.334758831926685
51	21.335098139311665	24.69821512088208	27.365599122080354	26.601087617725906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	158.0
1	422.0
2	686.0
3	1122.5
4	1559.0
5	1155.5
6	752.0
7	712.0
8	672.0
9	679.0
10	686.0
11	673.0
12	660.0
13	704.5
14	749.0
15	733.5
16	718.0
17	708.5
18	699.0
19	732.0
20	765.0
21	793.0
22	821.0
23	1035.0
24	1249.0
25	1483.5
26	2336.5
27	2955.0
28	3463.5
29	3972.0
30	4666.0
31	5360.0
32	5920.5
33	6481.0
34	7546.0
35	8611.0
36	8860.0
37	9109.0
38	9812.0
39	10515.0
40	12715.0
41	14915.0
42	16057.5
43	17200.0
44	18307.5
45	19415.0
46	25503.0
47	31591.0
48	30702.0
49	29813.0
50	29322.0
51	28831.0
52	25409.0
53	21987.0
54	20050.5
55	18114.0
56	17476.5
57	16839.0
58	16505.0
59	16171.0
60	15807.5
61	15444.0
62	13972.5
63	12501.0
64	10778.0
65	9055.0
66	7470.0
67	5885.0
68	4905.5
69	3926.0
70	3189.5
71	2453.0
72	2168.0
73	1883.0
74	1463.0
75	878.0
76	713.0
77	484.5
78	256.0
79	172.0
80	88.0
81	84.0
82	80.0
83	47.5
84	15.0
85	15.5
86	16.0
87	9.0
88	2.0
89	3.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	327137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.481376657752392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.60246405672248	21.91181387277353
2	9.016549753316596	5.6770679787504825
3	3.476593298030173	3.2834382930331643
4	2.212843823641877	2.786534795874037
5	1.7470802821988252	2.750024620761678
6	1.4733611293649065	2.7830061999856843
7	1.287565904377524	2.837404304517191
8	1.1447248185876875	2.883001054674901
9	1.0658127053004802	3.019792609996422
>10	8.83515778701531	44.85223772306774
>50	0.09289651488626852	2.0311194862836426
>100	0.038956603016822286	2.5770280523447053
>500	0.001997774513683194	0.46509145691492604
>1k	0.003995549027366388	2.142439551021901
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	2004	0.6125873869357487	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	1864	0.5697918609023132	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	1805	0.5517566035025081	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1140	0.34847785484368937	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	756	0.23109584058055185	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	723	0.2210083237298135	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	423	0.12930362508673734	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	421	0.12869226042911686	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	398	0.12166156686648102	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	364	0.11126836768693238	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	349	0.10668313275477859	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.056823288102538E-4	0.0	0.0	0.13327749536127065	0.0
2	3.056823288102538E-4	0.0	0.0	0.8528536973806081	0.0
3	3.056823288102538E-4	0.0	0.0	1.0970938781000008	0.0
4	3.056823288102538E-4	0.0	0.0	1.5161843508988588	0.0
5	3.056823288102538E-4	0.0	0.0	3.026560737550323	0.0
6	3.056823288102538E-4	0.0	0.0	3.619584455442215	0.0
7	3.056823288102538E-4	0.0	0.0	4.227280925117	0.0
8	3.056823288102538E-4	0.0	0.0	5.057514130165649	0.0
9	3.056823288102538E-4	0.0	0.0	5.313981604037452	0.0
10	3.056823288102538E-4	0.0	0.0	6.485661970367155	0.0
11	3.056823288102538E-4	0.0	0.0	7.4152419322791365	0.0
12	3.056823288102538E-4	0.0	0.0	8.726007758217506	0.0
13	3.056823288102538E-4	0.0	0.0	9.053393532373287	0.0
14	3.056823288102538E-4	0.0	0.0	9.20012105020221	0.0
15	3.056823288102538E-4	0.0	0.0	9.547987540388277	0.0
16	3.056823288102538E-4	0.0	0.0	10.101883920192458	0.0
17	3.056823288102538E-4	0.0	0.0	10.668007593149047	0.0
18	3.056823288102538E-4	0.0	0.0	11.290682496935535	0.0
19	3.056823288102538E-4	0.0	0.0	11.796892433445315	0.0
20	3.056823288102538E-4	0.0	0.0	12.195196507885075	0.0
21	3.056823288102538E-4	0.0	0.0	12.650663177812353	0.0
22	3.056823288102538E-4	0.0	0.0	13.176742465694801	0.0
23	3.056823288102538E-4	0.0	0.0	13.67011374439455	0.0
24	3.056823288102538E-4	0.0	0.0	14.070557595135982	0.0
25	3.056823288102538E-4	0.0	0.0	14.433096837104944	0.0
26	3.056823288102538E-4	0.0	0.0	14.779434915646961	0.0
27	3.056823288102538E-4	0.0	0.0	15.137083240354958	0.0
28	3.056823288102538E-4	0.0	0.0	15.51337818712038	0.0
29	3.056823288102538E-4	0.0	0.0	15.867969688540276	0.0
30	3.056823288102538E-4	0.0	0.0	16.287060161339134	0.0
31	3.056823288102538E-4	0.0	0.0	16.660298284816452	0.0
32	3.056823288102538E-4	0.0	0.0	17.02925685569043	0.0
33	3.056823288102538E-4	0.0	0.0	17.40463475546942	0.0
34	3.056823288102538E-4	0.0	0.0	17.77756719661793	0.0
35	3.056823288102538E-4	0.0	0.0	18.189626975854154	0.0
36	3.056823288102538E-4	0.0	0.0	18.58426286234819	0.0
37	3.056823288102538E-4	0.0	0.0	18.975841925554125	0.0
38	3.056823288102538E-4	0.0	0.0	19.34327208478405	0.0
39	3.056823288102538E-4	0.0	0.0	19.708256785383494	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	45	3.8380676E-10	45.000004	25
CTCACGA	45	3.8380676E-10	45.000004	24
CCTTCGT	20	7.025879E-4	45.0	33
TTGTGCG	35	1.2083729E-7	45.0	1
GACGTAG	20	7.025879E-4	45.0	1
TGCGGTC	20	7.025879E-4	45.0	15
CCCGTAA	20	7.025879E-4	45.0	43
CCGTAAC	20	7.025879E-4	45.0	44
TCTAAAC	20	7.025879E-4	45.0	34
GCGATAT	20	7.025879E-4	45.0	9
CCCCACG	20	7.025879E-4	45.0	38
CGTAACG	20	7.025879E-4	45.0	45
ACCCGTA	20	7.025879E-4	45.0	42
CAATCAC	20	7.025879E-4	45.0	42
ATCCGGT	25	3.884668E-5	44.999996	11
CGTAAGG	25	3.884668E-5	44.999996	2
TATCCGG	25	3.884668E-5	44.999996	10
TAGGGTA	125	0.0	43.2	5
TAACGGG	75	0.0	42.0	3
CACGGGC	65	0.0	41.538464	4
>>END_MODULE