##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935542.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592387
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.159724977084238	31.0	31.0	33.0	28.0	34.0
2	31.18651320842625	31.0	31.0	34.0	28.0	34.0
3	30.979569099254373	31.0	31.0	34.0	27.0	34.0
4	34.82333001905849	35.0	35.0	37.0	32.0	37.0
5	34.81838055190273	35.0	35.0	37.0	32.0	37.0
6	34.98205396134622	36.0	35.0	37.0	32.0	37.0
7	35.45663223534615	37.0	35.0	37.0	33.0	37.0
8	35.14624392500173	37.0	35.0	37.0	32.0	37.0
9	37.04304618433558	39.0	37.0	39.0	33.0	39.0
10	36.40468477532424	38.0	35.0	39.0	32.0	39.0
11	36.604981203166176	39.0	35.0	39.0	32.0	39.0
12	36.70644190368796	39.0	35.0	39.0	33.0	39.0
13	36.730279361295906	39.0	35.0	39.0	33.0	39.0
14	37.711490967897674	40.0	37.0	41.0	33.0	41.0
15	37.91222460992561	40.0	37.0	41.0	33.0	41.0
16	37.88652350574878	40.0	37.0	41.0	33.0	41.0
17	37.788032147903316	39.0	36.0	41.0	33.0	41.0
18	37.744189187136115	39.0	36.0	41.0	33.0	41.0
19	37.633931872238925	39.0	36.0	41.0	33.0	41.0
20	37.666277281574374	39.0	36.0	41.0	33.0	41.0
21	37.61495272516109	39.0	36.0	41.0	33.0	41.0
22	37.73791963699406	39.0	36.0	41.0	33.0	41.0
23	37.773163489408105	40.0	36.0	41.0	33.0	41.0
24	37.75179063686408	40.0	36.0	41.0	33.0	41.0
25	37.3539307918641	39.0	35.0	41.0	32.0	41.0
26	37.43012253813807	39.0	35.0	41.0	33.0	41.0
27	37.48611465140187	39.0	35.0	41.0	33.0	41.0
28	37.374638538658004	39.0	35.0	41.0	33.0	41.0
29	37.35053773968706	39.0	35.0	41.0	33.0	41.0
30	37.08149739950404	39.0	35.0	41.0	32.0	41.0
31	37.05599042517814	39.0	35.0	41.0	32.0	41.0
32	37.02092382175841	39.0	35.0	41.0	32.0	41.0
33	36.98497434953839	39.0	35.0	41.0	31.0	41.0
34	36.845779870253736	39.0	35.0	41.0	31.0	41.0
35	36.81312047698548	39.0	35.0	41.0	31.0	41.0
36	36.67385678618876	39.0	35.0	41.0	31.0	41.0
37	36.721572215460505	39.0	35.0	41.0	31.0	41.0
38	36.542849522356164	39.0	35.0	41.0	31.0	41.0
39	36.43867269200708	39.0	35.0	41.0	30.0	41.0
40	36.33637132482651	39.0	35.0	41.0	30.0	41.0
41	36.17188425809479	39.0	35.0	41.0	30.0	41.0
42	36.21713508230262	39.0	35.0	41.0	30.0	41.0
43	36.18737413211296	39.0	35.0	41.0	30.0	41.0
44	36.186273500262494	39.0	35.0	41.0	30.0	41.0
45	36.133134251764474	39.0	35.0	40.0	30.0	41.0
46	36.01969489539777	38.0	35.0	40.0	30.0	41.0
47	35.97685972176972	38.0	35.0	40.0	30.0	41.0
48	35.89711455518099	38.0	35.0	40.0	30.0	41.0
49	35.87627007344861	38.0	35.0	40.0	30.0	41.0
50	35.75318330753376	38.0	35.0	40.0	29.0	41.0
51	34.58300401595579	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	20.0
9	47.0
10	65.0
11	28.0
12	38.0
13	28.0
14	47.0
15	59.0
16	104.0
17	146.0
18	235.0
19	414.0
20	736.0
21	1230.0
22	1875.0
23	2624.0
24	3520.0
25	4796.0
26	5865.0
27	6805.0
28	7391.0
29	8097.0
30	10353.0
31	12641.0
32	17054.0
33	24374.0
34	43540.0
35	44863.0
36	49814.0
37	74646.0
38	126395.0
39	144520.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.53068686517428	20.084167951018507	22.70525855564015	12.679886628167058
2	34.664501415459824	24.18267112546359	23.834756670892506	17.318070788184077
3	32.880026064042596	24.21491356157377	26.395076191746274	16.509984182637364
4	28.818154348424258	24.691291334887495	26.99738515531232	19.493169161375924
5	25.824165621460292	29.471274690362886	24.560633504786566	20.143926183390253
6	25.142516631863966	34.76426727797875	25.176784770766407	14.916431319390872
7	77.17944519376691	6.278159378919524	11.562373921102251	4.980021506211312
8	77.62763193655499	7.363092032742109	9.899609545786792	5.109666484916111
9	71.68540160401899	7.792709833267779	12.014443260908832	8.507445301804395
10	41.31252036253328	26.032475391931285	18.07787814384853	14.577126101686904
11	31.44633491281882	24.931674732902646	24.82937674189339	18.792613612385146
12	28.31780575873542	22.329490687675456	28.899520077246798	20.453183476342325
13	26.5233707019229	22.730579840543427	30.625925281952508	20.120124175581168
14	20.80430529366782	27.602901481632784	29.918786198886878	21.674007025812518
15	19.843615744437333	25.008651439008624	34.144233414980405	21.003499401573634
16	23.694307943962308	25.164461745446808	30.037627429366275	21.103602881224603
17	24.115485316186884	24.69990057175461	28.673823024475553	22.510791087582948
18	24.508302849319787	24.835453850270177	29.12386666148987	21.532376638920166
19	25.148593740240756	25.99820725302885	26.618241116027193	22.234957890703207
20	26.49180350007681	26.22424192293214	27.820664531801004	19.463290045190053
21	25.66177178094725	26.711423444471265	28.214663724896056	19.412141049685424
22	23.928107807902606	24.44314274283534	28.766498927221562	22.86225052204049
23	23.978581569143145	25.76406977195651	28.906441228453694	21.35090743044665
24	23.658182910833627	24.775695617898435	28.498937350076893	23.067184121191044
25	24.152454392145675	26.653015680627696	26.60996949629212	22.58456043093451
26	22.324764047826843	25.70194821966046	27.756010851014622	24.217276881498076
27	23.417293087120413	25.215948358083484	28.708259971944017	22.658498582852086
28	21.13128748605219	26.444368293024663	28.741177642318284	23.683166578604865
29	24.208498836064937	25.571796815257592	27.911145923188723	22.30855842548874
30	24.491253184151578	24.142494686750386	28.453527845817007	22.91272428328103
31	24.254921191720953	24.77738370355865	27.22595195370594	23.741743151014454
32	26.46952076936192	25.103353044546893	27.26866052090947	21.158465665181716
33	24.918170047620897	24.119367913205387	27.964658238617663	22.997803800556056
34	23.650755333928664	24.375281699294547	29.55179637635532	22.42216659042146
35	23.12609831073268	25.019792804366066	28.75535756186412	23.098751323037135
36	23.464221868474493	26.82520041797001	28.11422262811304	21.596355085442458
37	23.478739405152375	26.05155075989176	27.75010255120386	22.71960728375201
38	22.7388514602785	26.277923046927093	28.373681394088663	22.609544098705744
39	24.218964967158293	24.06653083204054	27.90793856043431	23.806565640366856
40	24.1727114200683	22.82899523453418	30.251001456817928	22.747291888579593
41	20.494879192149725	24.223522798440882	29.91844858175483	25.36314942765456
42	22.480067928566967	24.761346889786576	29.591804006502503	23.166781175143953
43	22.653771943003477	23.75119643071168	28.91589450815092	24.679137118133923
44	22.657316922889937	24.130846895694873	28.476148193663942	24.735687987751252
45	22.86512026766286	22.9495245506738	27.881266807002852	26.304088374660484
46	23.089635660471956	24.92036455897918	27.901861452057524	24.08813832849134
47	21.782719742330606	24.314341806960652	30.253027159610184	23.649911291098554
48	21.210121086384408	24.28260579654854	30.018889678537846	24.488383438529205
49	22.61190741863005	23.705955735017817	30.570724880863352	23.111411965488777
50	21.66421612898325	23.656832442305454	30.24576839127125	24.433183037440052
51	21.416236345497115	23.087947574811736	28.612207897877568	26.883608181813578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	259.0
1	555.0
2	851.0
3	3146.0
4	5441.0
5	3605.0
6	1769.0
7	1754.0
8	1739.0
9	1680.0
10	1621.0
11	1615.0
12	1609.0
13	1601.0
14	1593.0
15	1637.0
16	1681.0
17	1636.5
18	1592.0
19	1596.0
20	1600.0
21	1698.0
22	1796.0
23	2086.5
24	2377.0
25	2834.0
26	3976.5
27	4662.0
28	5496.0
29	6330.0
30	7115.0
31	7900.0
32	9766.0
33	11632.0
34	13106.0
35	14580.0
36	15946.5
37	17313.0
38	18942.5
39	20572.0
40	22252.5
41	23933.0
42	25357.0
43	26781.0
44	30213.5
45	33646.0
46	42103.0
47	50560.0
48	50333.5
49	50107.0
50	50270.5
51	50434.0
52	45963.5
53	41493.0
54	39349.0
55	37205.0
56	35891.0
57	34577.0
58	32903.5
59	31230.0
60	30097.0
61	28964.0
62	26657.0
63	24350.0
64	20678.0
65	17006.0
66	14238.0
67	11470.0
68	9721.0
69	7972.0
70	6340.5
71	4709.0
72	4081.0
73	3453.0
74	2729.0
75	1616.0
76	1227.0
77	882.0
78	537.0
79	439.0
80	341.0
81	219.5
82	98.0
83	68.5
84	39.0
85	32.0
86	25.0
87	17.5
88	10.0
89	7.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	592387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.88163502092391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.87229781545128	24.35150962698267
2	8.772424317049584	5.944481579179034
3	3.4176958799798576	3.473913732539787
4	2.0415184841867036	2.7667993667873487
5	1.5469427948179157	2.6206475586134306
6	1.3231248869549765	2.689778070414584
7	1.130807043577758	2.681951407071412
8	0.9725885847531729	2.6362313163299143
9	0.8997553609238031	2.7436664472245966
>10	7.936544064365663	44.42852801279669
>50	0.05097296819812516	1.1772992146474193
>100	0.03028097120672777	1.9814877704148712
>500	0.002523414267227314	0.5661637908045943
>1k	0.002523414267227314	1.937542106193554
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3137	0.5295524716106194	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	2625	0.44312248580741975	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	2127	0.3590558199285265	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	1974	0.33322810932717967	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	1468	0.24781097492011134	TruSeq Adapter, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	722	0.12187978466779317	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	692	0.11681552768713695	TruSeq Adapter, Index 16 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	676	0.11411459063078698	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	613	0.1034796509714089	No Hit
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	608	0.10263560814129952	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.376171320437484E-4	0.0	0.0	0.10634939659378076	0.0
2	3.376171320437484E-4	0.0	0.0	0.5705729531539349	0.0
3	3.376171320437484E-4	0.0	0.0	0.8362776360723648	0.0
4	3.376171320437484E-4	0.0	0.0	1.2508714742220879	0.0
5	5.064256980656227E-4	0.0	0.0	2.504275076934504	0.0
6	5.064256980656227E-4	0.0	0.0	3.248720853090969	0.0
7	5.064256980656227E-4	0.0	0.0	3.8614959477503725	0.0
8	5.064256980656227E-4	0.0	0.0	4.711109460538466	0.0
9	5.064256980656227E-4	0.0	0.0	5.076411197409802	0.0
10	5.064256980656227E-4	0.0	0.0	6.070018417014553	0.0
11	5.064256980656227E-4	0.0	0.0	6.9964398273425985	0.0
12	5.064256980656227E-4	0.0	0.0	8.080359629769053	0.0
13	5.064256980656227E-4	0.0	0.0	8.420171273171086	0.0
14	5.064256980656227E-4	0.0	0.0	8.589148647758982	0.0
15	5.064256980656227E-4	0.0	0.0	8.885238872561349	0.0
16	5.064256980656227E-4	0.0	0.0	9.371407542704347	0.0
17	5.064256980656227E-4	0.0	0.0	9.903829759937338	0.0
18	5.064256980656227E-4	0.0	0.0	10.488245015505067	0.0
19	5.064256980656227E-4	0.0	0.0	10.915161878974386	0.0
20	5.064256980656227E-4	0.0	0.0	11.277087444525286	0.0
21	5.064256980656227E-4	0.0	0.0	11.695901496825554	0.0
22	6.752342640874968E-4	0.0	0.0	12.176668292855853	0.0
23	6.752342640874968E-4	0.0	0.0	12.620803630059404	0.0
24	6.752342640874968E-4	0.0	0.0	12.996402689458074	0.0
25	6.752342640874968E-4	0.0	0.0	13.340265738444632	0.0
26	6.752342640874968E-4	0.0	0.0	13.68412878743119	0.0
27	6.752342640874968E-4	0.0	0.0	14.030692773474097	0.0
28	6.752342640874968E-4	0.0	0.0	14.382827442195726	0.0
29	6.752342640874968E-4	0.0	0.0	14.726521682616262	0.0
30	6.752342640874968E-4	0.0	0.0	15.189394770648242	0.0
31	8.44042830109371E-4	0.0	0.0	15.553683656123447	0.0
32	8.44042830109371E-4	0.0	0.0	15.937385526691166	0.0
33	8.44042830109371E-4	0.0	0.0	16.313828628919946	0.0
34	8.44042830109371E-4	0.0	0.0	16.684194622771937	0.0
35	8.44042830109371E-4	0.0	0.0	17.079881901527212	0.0
36	8.44042830109371E-4	0.0	0.0	17.4517671724734	0.0
37	8.44042830109371E-4	0.0	0.0	17.836650703003272	0.0
38	8.44042830109371E-4	0.0	0.0	18.25343905251128	0.0
39	8.44042830109371E-4	0.0	0.0	18.79463931517741	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCG	20	7.030515E-4	45.0	1
ACGTATG	20	7.030515E-4	45.0	1
ATATGCG	30	2.1637134E-6	44.999996	1
CGTTTTT	1675	0.0	41.64179	1
CACGACC	110	0.0	40.909092	27
AACACGT	105	0.0	40.714287	41
ATAGCGG	95	0.0	40.263157	2
CGGGTAT	45	1.9255822E-8	40.0	6
ACACGAC	120	0.0	39.374996	26
ACGGGAT	170	0.0	38.38235	5
CGTTAGG	30	1.13925424E-4	37.499996	2
AAGCACG	30	1.13925424E-4	37.499996	1
CACGGGA	145	0.0	37.241383	4
ACACGTG	115	0.0	37.173912	42
CCTATGC	115	0.0	37.173912	35
GCGAGAC	125	0.0	36.0	21
ACCGGTA	25	0.002106286	36.0	41
CGTATGT	25	0.002106286	36.0	17
CTTCCGT	25	0.002106286	36.0	24
CGTGCGG	100	0.0	36.0	2
>>END_MODULE