##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935541.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441957
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16495043635467	31.0	31.0	33.0	28.0	34.0
2	31.16509298415909	31.0	31.0	34.0	28.0	34.0
3	30.95232341607894	31.0	31.0	34.0	27.0	34.0
4	34.72583758148417	35.0	35.0	37.0	32.0	37.0
5	34.81052455329365	35.0	35.0	37.0	32.0	37.0
6	35.019861660749804	36.0	35.0	37.0	32.0	37.0
7	35.451982885212814	37.0	35.0	37.0	33.0	37.0
8	35.27978966279525	37.0	35.0	37.0	33.0	37.0
9	37.22444038673446	39.0	37.0	39.0	34.0	39.0
10	36.43951334632102	38.0	35.0	39.0	32.0	39.0
11	36.72813871032702	39.0	37.0	39.0	32.0	39.0
12	36.93631733403929	39.0	37.0	39.0	33.0	39.0
13	37.05574750484776	39.0	37.0	39.0	33.0	39.0
14	38.127252198743314	40.0	37.0	41.0	33.0	41.0
15	38.31799473704456	40.0	38.0	41.0	34.0	41.0
16	38.24885000124446	40.0	37.0	41.0	34.0	41.0
17	38.171435230124196	40.0	37.0	41.0	34.0	41.0
18	38.110718916093646	39.0	37.0	41.0	34.0	41.0
19	37.92465104071211	39.0	37.0	41.0	34.0	41.0
20	37.96757150582523	39.0	37.0	41.0	34.0	41.0
21	37.9243455811312	39.0	36.0	41.0	34.0	41.0
22	38.04489124507588	40.0	37.0	41.0	34.0	41.0
23	38.106157386352066	40.0	37.0	41.0	34.0	41.0
24	38.07796007303878	40.0	37.0	41.0	34.0	41.0
25	37.661283790051975	39.0	36.0	41.0	33.0	41.0
26	37.77207284871605	40.0	36.0	41.0	33.0	41.0
27	37.829254882262305	40.0	36.0	41.0	33.0	41.0
28	37.72518367171467	40.0	36.0	41.0	33.0	41.0
29	37.690997087952	40.0	36.0	41.0	33.0	41.0
30	37.383804306753824	39.0	36.0	41.0	33.0	41.0
31	37.29568487432035	39.0	36.0	41.0	32.0	41.0
32	37.23395036168677	39.0	36.0	41.0	32.0	41.0
33	37.118676703842226	40.0	36.0	41.0	31.0	41.0
34	36.97522835932003	40.0	36.0	41.0	31.0	41.0
35	36.953038417764624	40.0	36.0	41.0	31.0	41.0
36	36.788884438983885	40.0	36.0	41.0	31.0	41.0
37	36.77010659408042	40.0	36.0	41.0	31.0	41.0
38	36.63815710578179	39.0	35.0	41.0	30.0	41.0
39	36.60216039116928	39.0	35.0	41.0	30.0	41.0
40	36.5230961383121	39.0	35.0	41.0	30.0	41.0
41	36.40454840629292	39.0	35.0	41.0	30.0	41.0
42	36.43714207490774	39.0	35.0	41.0	30.0	41.0
43	36.39244541889822	39.0	35.0	41.0	30.0	41.0
44	36.38389255063275	39.0	35.0	41.0	30.0	41.0
45	36.35060424430431	39.0	35.0	41.0	30.0	41.0
46	36.244618820382975	39.0	35.0	41.0	29.0	41.0
47	36.22444038673446	39.0	35.0	41.0	29.0	41.0
48	36.15677995823123	39.0	35.0	41.0	29.0	41.0
49	36.14759580683189	39.0	35.0	41.0	29.0	41.0
50	36.040454161830226	39.0	35.0	41.0	29.0	41.0
51	34.892233859855146	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	8.0
9	26.0
10	27.0
11	21.0
12	15.0
13	9.0
14	18.0
15	20.0
16	44.0
17	68.0
18	128.0
19	270.0
20	466.0
21	736.0
22	1143.0
23	1674.0
24	2651.0
25	4075.0
26	5258.0
27	5503.0
28	5580.0
29	5794.0
30	6770.0
31	8397.0
32	10761.0
33	14860.0
34	24624.0
35	30681.0
36	35618.0
37	56034.0
38	102758.0
39	117903.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.14724735664329	21.731525917679775	24.436087673687712	14.685139051989221
2	35.28895345022253	23.704794810354855	24.66190149720448	16.344350242218134
3	30.897802274881947	22.935262932819256	30.236878248336374	15.93005654396242
4	27.09630122387472	24.95152243317789	29.740223596413223	18.211952746534166
5	24.23425808393124	29.883223933550095	27.380718033654862	18.501799948863802
6	23.345257570306615	34.065757528447335	27.869453363109987	14.719531538136064
7	77.30955726462076	5.20276859513482	12.683586864785488	4.804087275458925
8	78.37300913889813	5.119502576042466	11.587552635211118	4.919935649848289
9	72.74644365854597	7.03009568804205	13.55969019610505	6.663770457306932
10	34.26690831913512	30.128270397346345	21.59259837495503	14.012222908563505
11	24.245118869030243	25.305855547032856	31.13289301900411	19.316132564932786
12	22.32615390185018	22.473000767042947	34.97964734125718	20.22119798984969
13	22.189489022687727	23.77652124527952	36.226148697723985	17.80784103430877
14	19.185350611032295	27.857913778942294	33.04755892541582	19.90917668460959
15	18.19068370904862	27.05331061619117	36.36507624044873	18.39092943431148
16	20.457872598465464	26.490586188249083	33.23219227209887	19.819348941186586
17	20.699525066918277	25.94777319965517	31.558273768715058	21.7944279647115
18	21.460006290204703	25.974246363333993	32.76336838199192	19.802378964469394
19	20.863115642472003	28.164504691632896	30.822908110970072	20.14947155492503
20	21.932450442011326	27.10784080804241	31.416631029715557	19.543077720230702
21	21.585584117911925	27.628027161013403	31.983428252069775	18.802960469004905
22	20.609471057139043	24.90988942363171	31.546507918191136	22.93413160103811
23	19.11136151254534	27.58051122620526	31.58135293705044	21.72677432419896
24	20.43434089741762	25.570134651108596	32.33866643134966	21.65685802012413
25	19.800342567263332	27.748174596171122	30.435540109105634	22.01594272745991
26	19.79762737098858	27.980323877662304	30.70728600293694	21.514762748412174
27	21.004079582402813	27.82171116194562	30.56858472656842	20.605624529083148
28	19.126521358412695	27.213054663689	32.00062449514319	21.65979948275511
29	20.874655226639696	25.760650923053603	31.04578047185586	22.318913378450844
30	21.011546372158378	27.22323664971932	30.640989960561775	21.124227017560532
31	20.272334186357497	27.965842830863636	28.24301911724444	23.51880386553443
32	20.482988164006905	29.98051846672866	27.937107003622526	21.599386365641905
33	19.410033102767915	28.228764336801998	29.093554350310097	23.26764821011999
34	19.176299956783126	27.068922994770983	29.305113393384424	24.449663655061464
35	19.553712238973475	29.012550994780035	28.13463753261064	23.29909923363585
36	20.48796602384395	28.29551291188962	28.10793810257559	23.108582961690843
37	18.76607905293954	29.54110920293151	28.940598293499143	22.752213450629814
38	18.305627018013066	29.24854680432712	28.583097450656965	23.862728727002853
39	20.134990508126357	26.823423998262275	29.154193733779532	23.88739175983184
40	20.903164787524577	26.165893967060143	30.409293211783044	22.52164803363223
41	18.863373586118108	27.262606995703205	28.813662867654543	25.060356550524148
42	19.939722642700534	27.598160001991147	29.058030532382112	23.404086822926214
43	20.65155659939768	26.62815613283645	29.019565251823142	23.700722015942727
44	20.23047491045509	26.57724620268488	28.650751091169504	24.54152779569053
45	20.17526591953516	25.676479838536327	28.957115737503873	25.191138504424636
46	21.60097023013551	26.590143384989943	28.54779989908521	23.261086485789342
47	19.09280767133454	26.700787633186035	30.757969666732283	23.44843502874714
48	19.15163692395414	26.27223915448788	29.52911708605136	25.047006835506625
49	19.796043506494975	24.736795661116354	31.29354213192686	24.17361870046181
50	19.061356647818677	24.701045576832133	31.33404380969189	24.903553965657295
51	18.965872245489944	24.162531649006578	29.24175881364024	27.629837291863236
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	160.0
1	285.0
2	410.0
3	3099.5
4	5789.0
5	3938.5
6	2088.0
7	2080.5
8	2073.0
9	2077.0
10	2081.0
11	2022.0
12	1963.0
13	1910.0
14	1857.0
15	1775.5
16	1694.0
17	1689.5
18	1685.0
19	1706.0
20	1727.0
21	1782.5
22	1838.0
23	1955.5
24	2073.0
25	2550.5
26	3722.0
27	4416.0
28	4971.0
29	5526.0
30	6478.5
31	7431.0
32	8909.0
33	10387.0
34	11940.5
35	13494.0
36	14584.0
37	15674.0
38	17501.5
39	19329.0
40	20729.5
41	22130.0
42	25090.0
43	28050.0
44	30466.5
45	32883.0
46	40046.5
47	47210.0
48	48336.5
49	49463.0
50	47315.0
51	45167.0
52	37705.0
53	30243.0
54	26854.0
55	23465.0
56	19956.5
57	16448.0
58	14797.0
59	13146.0
60	11707.0
61	10268.0
62	8665.0
63	7062.0
64	6091.0
65	5120.0
66	3956.0
67	2792.0
68	2161.5
69	1531.0
70	1297.5
71	1064.0
72	816.0
73	568.0
74	432.0
75	247.0
76	198.0
77	133.5
78	69.0
79	49.5
80	30.0
81	25.0
82	20.0
83	13.5
84	7.0
85	5.0
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	441957.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.58023396638824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.33790800407914	32.39663423389614
2	9.72219711247426	8.473912496579448
3	3.926868169445229	5.134015007387574
4	2.2498921981547695	3.922033135789456
5	1.5982935154828155	3.4827002675851126
6	1.274242430767652	3.331906995765214
7	1.027499191219756	3.134505860754216
8	0.8588015592272572	2.994141830545832
9	0.7466024493297545	2.9283398479502303
>10	4.192331859682243	28.068525136726088
>50	0.04392427881216905	1.3550016245087095
>100	0.014641426270721515	1.2319439593160997
>500	0.003137448486583182	0.985600744365914
>1k	0.003660356567680379	2.5607388588300064
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3607	0.8161427469188178	No Hit
GCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	1409	0.3188092959269793	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTC	1359	0.30749597811551804	No Hit
CCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	1318	0.29821905751011973	No Hit
CGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTG	1229	0.27808135180571864	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTC	1162	0.2629215059383605	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTT	1153	0.2608851087322975	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCG	913	0.20658118323728328	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGC	909	0.20567611781236636	No Hit
CTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGCT	820	0.18553841210796523	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCT	602	0.136212346449994	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCC	552	0.12489902863853271	No Hit
CGTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCT	529	0.11969490244526051	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.787990686876777E-4	0.0	0.0	0.1930052018635297	0.0
2	6.787990686876777E-4	0.0	0.0	1.0019074253830125	0.0
3	6.787990686876777E-4	0.0	0.0	1.5496982738139684	0.0
4	6.787990686876777E-4	0.0	0.0	2.1705731553069643	0.0
5	6.787990686876777E-4	0.0	0.0	3.913729163696921	0.0
6	6.787990686876777E-4	0.0	0.0	5.1844410202802536	0.0
7	6.787990686876777E-4	0.0	0.0	6.206259885011438	0.0
8	6.787990686876777E-4	0.0	0.0	7.641467382573418	0.0
9	6.787990686876777E-4	0.0	0.0	8.220256721807777	0.0
10	6.787990686876777E-4	0.0	0.0	9.526039863606641	0.0
11	6.787990686876777E-4	0.0	0.0	11.074606805639462	0.0
12	6.787990686876777E-4	0.0	0.0	12.51614976117586	0.0
13	6.787990686876777E-4	0.0	0.0	13.076611525555654	0.0
14	6.787990686876777E-4	0.0	0.0	13.341795695056307	0.0
15	6.787990686876777E-4	0.0	0.0	13.700880402392087	0.0
16	6.787990686876777E-4	0.0	0.0	14.424706475969382	0.0
17	6.787990686876777E-4	0.0	0.0	15.416658181678308	0.0
18	6.787990686876777E-4	0.0	0.0	16.435309317422284	0.0
19	6.787990686876777E-4	0.0	0.0	17.08492002615639	0.0
20	6.787990686876777E-4	0.0	0.0	17.670723622433858	0.0
21	6.787990686876777E-4	0.0	0.0	18.389798102530335	0.0
22	6.787990686876777E-4	0.0	0.0	19.196437662487526	0.0
23	6.787990686876777E-4	0.0	0.0	19.994026568195547	0.0
24	6.787990686876777E-4	0.0	0.0	20.600872935602332	0.0
25	6.787990686876777E-4	0.0	0.0	21.132146340028555	0.0
26	6.787990686876777E-4	0.0	0.0	21.653237758424464	0.0
27	6.787990686876777E-4	0.0	0.0	22.180664634794788	0.0
28	6.787990686876777E-4	0.0	0.0	22.725287754238533	0.0
29	6.787990686876777E-4	0.0	0.0	23.264933013845237	0.0
30	6.787990686876777E-4	0.0	0.0	23.90752946553624	0.0
31	6.787990686876777E-4	0.0	0.0	24.510076772174667	0.0
32	6.787990686876777E-4	0.0	0.0	25.0847933169969	0.0
33	6.787990686876777E-4	0.0	0.0	25.64729147858276	0.0
34	9.050654249169037E-4	0.0	0.0	26.195534859726173	0.0
35	9.050654249169037E-4	0.0	0.0	26.75192382969384	0.0
36	0.0011313317811461295	0.0	0.0	27.312385594073632	0.0
37	0.0011313317811461295	0.0	0.0	27.86538056869786	0.0
38	0.0011313317811461295	0.0	0.0	28.417470477897172	0.0
39	0.0011313317811461295	0.0	0.0	28.979742373126797	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	20	7.0285704E-4	45.0	31
AAATCGG	20	7.0285704E-4	45.0	2
GTCGAAT	20	7.0285704E-4	45.0	30
GAGTACG	25	3.886895E-5	45.0	1
GCGCCAG	20	7.0285704E-4	45.0	1
ACGTGCG	20	7.0285704E-4	45.0	1
ATGCGAG	20	7.0285704E-4	45.0	1
CGATGCG	25	3.886895E-5	45.0	10
TCGAATT	20	7.0285704E-4	45.0	31
CTAGCGG	50	2.1827873E-11	45.0	2
CGGACGG	25	3.886895E-5	45.0	2
TCGTGCG	30	2.1624583E-6	44.999996	1
GCTAGCG	30	2.1624583E-6	44.999996	1
TTTGCGG	60	0.0	44.999996	2
CGTTTTT	2230	0.0	43.58744	1
CGTGCGG	60	3.6379788E-12	41.249996	2
TACGGGA	210	0.0	40.714287	4
CGCGAGG	50	1.0786607E-9	40.5	2
CGTTTCT	140	0.0	40.17857	1
ACGGGAC	185	0.0	40.135136	5
>>END_MODULE