SRR2935539.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935539.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501537
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC	2285	0.45559948717641974	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGC	1910	0.3808293306376199	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCG	1787	0.35630471929289365	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1699	0.33875865589178866	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	1268	0.25282282264319483	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	634	0.12641141132159742	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT	521	0.10388067081790575	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	30	2.1630458E-6	45.000004	1
CCCGCAT	30	2.1630458E-6	45.000004	23
CGAATAT	25	3.8876497E-5	45.0	14
AGTACGG	35	1.2101009E-7	45.0	2
TAGTACG	20	7.029479E-4	45.0	1
CGTAAGG	80	0.0	42.1875	2
CGTTAGG	75	0.0	39.000004	2
CGTTTTT	1025	0.0	38.853657	1
ACGCAAC	35	6.241249E-6	38.571426	39
CCGCATG	35	6.241249E-6	38.571426	24
ACGGGAT	170	0.0	38.38235	5
TTGGACG	30	1.13900445E-4	37.500004	1
TACGAAT	30	1.13900445E-4	37.500004	12
AAACCGG	30	1.13900445E-4	37.500004	2
ATACGGA	30	1.13900445E-4	37.500004	4
TAGGGAT	205	0.0	37.317074	5
CGCACGG	85	0.0	37.058823	2
TAGCGAG	55	2.743036E-9	36.81818	1
CGGGACC	135	0.0	36.666668	6
GTTTGCG	25	0.0021059786	36.0	1