##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935539.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501537
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.230367849231463	31.0	31.0	33.0	28.0	34.0
2	31.259450449318795	31.0	31.0	34.0	28.0	34.0
3	31.04803833017305	31.0	31.0	34.0	27.0	34.0
4	34.906020891778674	35.0	35.0	37.0	32.0	37.0
5	34.890817626615785	35.0	35.0	37.0	33.0	37.0
6	35.05347561595655	37.0	35.0	37.0	32.0	37.0
7	35.5439738244636	37.0	35.0	37.0	33.0	37.0
8	35.28694194047498	37.0	35.0	37.0	33.0	37.0
9	37.164065662154535	39.0	37.0	39.0	34.0	39.0
10	36.431258311949065	38.0	35.0	39.0	32.0	39.0
11	36.673709018477204	39.0	35.0	39.0	32.0	39.0
12	36.77865042858254	39.0	35.0	39.0	33.0	39.0
13	36.79840370700467	39.0	35.0	39.0	33.0	39.0
14	37.79516167301715	40.0	37.0	41.0	33.0	41.0
15	37.996353210231746	40.0	37.0	41.0	33.0	41.0
16	37.9863559418348	40.0	37.0	41.0	33.0	41.0
17	37.886199821747944	40.0	37.0	41.0	33.0	41.0
18	37.853490370600774	39.0	37.0	41.0	33.0	41.0
19	37.718852248189066	39.0	36.0	41.0	33.0	41.0
20	37.81595176427661	39.0	36.0	41.0	33.0	41.0
21	37.75051890488638	39.0	36.0	41.0	33.0	41.0
22	37.87516574051366	40.0	36.0	41.0	33.0	41.0
23	37.913160943260415	40.0	36.0	41.0	34.0	41.0
24	37.90497211571629	40.0	36.0	41.0	33.0	41.0
25	37.50610024783815	39.0	35.0	41.0	33.0	41.0
26	37.598520149061784	39.0	36.0	41.0	33.0	41.0
27	37.66786498304213	40.0	36.0	41.0	33.0	41.0
28	37.56004641731318	40.0	36.0	41.0	33.0	41.0
29	37.55012292213735	40.0	36.0	41.0	33.0	41.0
30	37.277447127529975	39.0	35.0	41.0	32.0	41.0
31	37.27736737269633	39.0	35.0	41.0	32.0	41.0
32	37.23990852120581	39.0	35.0	41.0	32.0	41.0
33	37.24188245333844	40.0	35.0	41.0	32.0	41.0
34	37.14303830026498	40.0	35.0	41.0	32.0	41.0
35	37.13741159675158	40.0	35.0	41.0	32.0	41.0
36	37.00556888125901	40.0	35.0	41.0	32.0	41.0
37	37.0311462564078	40.0	35.0	41.0	32.0	41.0
38	36.85449926924634	39.0	35.0	41.0	31.0	41.0
39	36.78239691189284	39.0	35.0	41.0	31.0	41.0
40	36.675623134484596	39.0	35.0	41.0	31.0	41.0
41	36.529356757327974	39.0	35.0	41.0	31.0	41.0
42	36.55546250824964	39.0	35.0	41.0	31.0	41.0
43	36.523090021274605	39.0	35.0	41.0	31.0	41.0
44	36.50906712764961	39.0	35.0	41.0	31.0	41.0
45	36.47234002675775	39.0	35.0	41.0	31.0	41.0
46	36.37251488923051	39.0	35.0	41.0	31.0	41.0
47	36.3332296520496	39.0	35.0	41.0	31.0	41.0
48	36.27168683467023	38.0	35.0	40.0	31.0	41.0
49	36.25473494477975	38.0	35.0	40.0	31.0	41.0
50	36.12909316760279	38.0	35.0	40.0	30.0	41.0
51	34.971740868570016	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	15.0
9	49.0
10	31.0
11	30.0
12	21.0
13	28.0
14	34.0
15	37.0
16	58.0
17	104.0
18	164.0
19	283.0
20	497.0
21	947.0
22	1357.0
23	1918.0
24	2636.0
25	3412.0
26	4151.0
27	4722.0
28	5217.0
29	6261.0
30	7634.0
31	10052.0
32	13695.0
33	19990.0
34	35623.0
35	37621.0
36	41728.0
37	63519.0
38	110404.0
39	129281.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.72085409451346	20.370780221598807	22.214711975387658	11.69365370850007
2	34.02640283767698	24.19761652679663	23.51491515082636	18.26106548470003
3	33.07133870482138	24.5126481196801	25.714752849739895	16.701260325758618
4	28.752415076056202	24.853400646412926	26.070060633612275	20.324123643918593
5	25.86788213033136	29.87915148832489	24.303889842623775	19.949076538719975
6	25.35645425960597	35.45860026279218	24.59938150126511	14.58556397633674
7	78.92378827484313	5.847823789670552	10.552362039091832	4.676025896394483
8	79.6928242582302	6.607687967188862	8.828860084101473	4.870627690479466
9	73.94090565601341	7.144437997595392	10.839080666032617	8.075575680358577
10	40.835072985642135	25.221668590752028	18.59005417347075	15.353204250135086
11	30.99711486889302	25.793311360876665	23.930637221182085	19.278936549048225
12	27.025722927720185	23.623780498746854	28.656709275686538	20.69378729784642
13	26.21062852790522	23.75936371593721	31.047360414087095	18.982647342070475
14	21.76030881071586	26.99521670385236	29.600607731832344	21.643866753599436
15	20.005503083521255	25.710366333889624	33.20094030948863	21.083190273100488
16	24.65002582062739	25.538095893224227	28.702169530862133	21.10970875528625
17	24.962864155585727	24.979413283566316	27.300877103782973	22.756845457064983
18	25.19594765690268	23.98028460512385	29.317278685321323	21.506489052652146
19	24.238490879037837	26.24911023513719	26.881566065913383	22.630832819911593
20	26.62894263035429	25.966379349878473	27.49188993035409	19.912788089413144
21	25.38337151595994	27.31802439301587	28.053563346273556	19.245040744750636
22	24.76905990983716	23.79724726191687	28.397904840520237	23.03578798772573
23	23.040573277744215	26.301349651172295	28.56638692658767	22.09169014449582
24	23.316126227975204	25.039428795881456	28.87144916526597	22.772995810877365
25	23.148242303160085	27.37205829280791	27.088131085044576	22.39156831898743
26	22.23106171628414	26.02639486219362	27.83682958585309	23.905713835669154
27	23.86763090260539	25.19016543146368	28.012888381116447	22.929315284814482
28	20.55282062938527	26.399448096551204	29.575285572151206	23.472445701912324
29	22.275923810207424	26.06906369819176	28.937047515936015	22.717964975664806
30	24.39979503007754	25.00094708864949	29.06126566933247	21.537992211940495
31	24.244671878645043	25.00333973365873	27.943102901680234	22.80888548601599
32	24.079579373007377	25.85731461487388	27.822473715797635	22.24063229632111
33	24.457218510299338	25.214091881556094	26.28938642612609	24.039303182018475
34	21.898683447083663	25.079904373954463	29.49493257725751	23.526479601704363
35	22.281107874394113	25.667298723723274	29.248689528389733	22.802903873492884
36	24.217555235206977	27.517810251287543	27.454604545626744	20.81002996787874
37	23.242153619772818	26.222392365867325	27.368668712378145	23.166785301981708
38	22.279512777721287	27.643623501356828	27.17306998287265	22.903793738049234
39	23.16977610824326	25.04700550507739	26.44909548049296	25.334122906186384
40	24.148168529938967	24.366696774116367	29.00603544703581	22.479099248908856
41	21.868775384468144	24.436282866468474	28.202106723930637	25.49283502513274
42	23.140266819795947	24.207386493917696	29.37390461720671	23.27844206907965
43	23.672231560183995	24.101312565174656	28.230818464041533	23.995637410599816
44	22.819652388557575	24.25005532991584	28.18914656346391	24.741145718062675
45	22.23504945796621	23.45729228352046	27.81649210327453	26.491166155238794
46	23.315328679638792	25.506792121019984	27.367871164041734	23.81000803529949
47	21.056671790914727	25.435411544910945	30.215916273375644	23.292000390798684
48	21.85681215942194	25.27550310345996	29.00025322159681	23.867431515521286
49	23.43216951092342	23.616203789550923	29.780056107525464	23.17157059200019
50	21.641673495674297	24.318046325595123	29.784642010459844	24.255638168270735
51	21.52004737437118	23.35161712894562	28.288441331347435	26.83989416533576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	197.0
1	402.0
2	607.0
3	1869.5
4	3132.0
5	2119.0
6	1106.0
7	1071.5
8	1037.0
9	1041.5
10	1046.0
11	1038.5
12	1031.0
13	1034.0
14	1037.0
15	1019.5
16	1002.0
17	1061.0
18	1120.0
19	1108.0
20	1096.0
21	1209.0
22	1322.0
23	1519.5
24	1717.0
25	2226.0
26	3152.5
27	3570.0
28	4418.0
29	5266.0
30	5910.0
31	6554.0
32	8103.5
33	9653.0
34	10887.5
35	12122.0
36	13129.0
37	14136.0
38	15918.5
39	17701.0
40	19227.0
41	20753.0
42	22740.5
43	24728.0
44	27905.0
45	31082.0
46	38381.0
47	45680.0
48	45627.0
49	45574.0
50	46310.0
51	47046.0
52	43033.5
53	39021.0
54	35627.0
55	32233.0
56	29918.5
57	27604.0
58	26025.0
59	24446.0
60	22779.0
61	21112.0
62	18927.5
63	16743.0
64	15020.0
65	13297.0
66	11163.0
67	9029.0
68	7692.5
69	6356.0
70	5086.0
71	3816.0
72	3173.0
73	2530.0
74	2052.5
75	1200.0
76	825.0
77	680.5
78	536.0
79	377.0
80	218.0
81	149.0
82	80.0
83	59.5
84	39.0
85	24.0
86	9.0
87	7.5
88	6.0
89	5.0
90	4.0
91	4.0
92	4.0
93	4.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	501537.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.984425990770134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59785316168396	25.048097950329602
2	8.780994037559795	6.14396072064809
3	3.451819896856377	3.622798131451192
4	2.1377949983404667	2.991581236115225
5	1.6499087660508986	2.886055555871527
6	1.4287737247459054	2.9990897178558065
7	1.257482642989996	3.0794615920853072
8	1.040966783955274	2.9134100329706634
9	0.9546078286890157	3.0056766239682577
>10	7.616254659702058	42.04030258724077
>50	0.046669127265728874	1.0989432361312539
>100	0.03284123770551281	2.1339897360457734
>500	0.0011523241300179935	0.23280987485906
>1k	0.0028808103250449833	1.8038230044274701
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC	2285	0.45559948717641974	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGC	1910	0.3808293306376199	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCG	1787	0.35630471929289365	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1699	0.33875865589178866	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	1268	0.25282282264319483	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	634	0.12641141132159742	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT	521	0.10388067081790575	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.975483364138638E-4	0.0	0.0	0.10108925164045723	0.0
2	7.975483364138638E-4	0.0	0.0	0.582409672666224	0.0
3	7.975483364138638E-4	0.0	0.0	0.8220729477585901	0.0
4	7.975483364138638E-4	0.0	0.0	1.223439148058867	0.0
5	7.975483364138638E-4	0.0	0.0	2.5192558076472924	0.0
6	7.975483364138638E-4	0.0	0.0	3.2563898575778056	0.0
7	7.975483364138638E-4	0.0	0.0	3.8864530433447584	0.0
8	7.975483364138638E-4	0.0	0.0	4.765949471325147	0.0
9	7.975483364138638E-4	0.0	0.0	5.080382902956313	0.0
10	7.975483364138638E-4	0.0	0.0	6.1494964479190966	0.0
11	7.975483364138638E-4	0.0	0.0	7.162980996417014	0.0
12	7.975483364138638E-4	0.0	0.0	8.318030374628393	0.0
13	7.975483364138638E-4	0.0	0.0	8.695669511920357	0.0
14	7.975483364138638E-4	0.0	0.0	8.84540921208206	0.0
15	7.975483364138638E-4	0.0	0.0	9.185762964646676	0.0
16	7.975483364138638E-4	0.0	0.0	9.744844348472794	0.0
17	7.975483364138638E-4	0.0	0.0	10.373113050482816	0.0
18	7.975483364138638E-4	0.0	0.0	11.058406458546429	0.0
19	7.975483364138638E-4	0.0	0.0	11.520785106582366	0.0
20	7.975483364138638E-4	0.0	0.0	11.965019529964888	0.0
21	9.969354205173297E-4	0.0	0.0	12.460496433962001	0.0
22	9.969354205173297E-4	0.0	0.0	12.98687833599515	0.0
23	9.969354205173297E-4	0.0	0.0	13.49710988421592	0.0
24	9.969354205173297E-4	0.0	0.0	13.936160243411752	0.0
25	9.969354205173297E-4	0.0	0.0	14.341514185394098	0.0
26	9.969354205173297E-4	0.0	0.0	14.712374161826546	0.0
27	9.969354205173297E-4	0.0	0.0	15.11015139461296	0.0
28	9.969354205173297E-4	0.0	0.0	15.4863948223162	0.0
29	9.969354205173297E-4	0.0	0.0	15.892346925550857	0.0
30	9.969354205173297E-4	0.0	0.0	16.41314598922911	0.0
31	9.969354205173297E-4	0.0	0.0	16.823085834145836	0.0
32	9.969354205173297E-4	0.0	0.0	17.23222813072615	0.0
33	9.969354205173297E-4	0.0	0.0	17.63160046018539	0.0
34	9.969354205173297E-4	0.0	0.0	18.028181370467184	0.0
35	9.969354205173297E-4	0.0	0.0	18.463044600896843	0.0
36	9.969354205173297E-4	0.0	0.0	18.882555025850536	0.0
37	0.0011963225046207958	0.0	0.0	19.28731080658057	0.0
38	0.0011963225046207958	0.0	0.0	19.73872316499082	0.0
39	0.0011963225046207958	0.0	0.0	20.337283191469425	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	30	2.1630458E-6	45.000004	1
CCCGCAT	30	2.1630458E-6	45.000004	23
CGAATAT	25	3.8876497E-5	45.0	14
AGTACGG	35	1.2101009E-7	45.0	2
TAGTACG	20	7.029479E-4	45.0	1
CGTAAGG	80	0.0	42.1875	2
CGTTAGG	75	0.0	39.000004	2
CGTTTTT	1025	0.0	38.853657	1
ACGCAAC	35	6.241249E-6	38.571426	39
CCGCATG	35	6.241249E-6	38.571426	24
ACGGGAT	170	0.0	38.38235	5
TTGGACG	30	1.13900445E-4	37.500004	1
TACGAAT	30	1.13900445E-4	37.500004	12
AAACCGG	30	1.13900445E-4	37.500004	2
ATACGGA	30	1.13900445E-4	37.500004	4
TAGGGAT	205	0.0	37.317074	5
CGCACGG	85	0.0	37.058823	2
TAGCGAG	55	2.743036E-9	36.81818	1
CGGGACC	135	0.0	36.666668	6
GTTTGCG	25	0.0021059786	36.0	1
>>END_MODULE