##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935538.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	393906
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.049773296167107	31.0	30.0	33.0	28.0	34.0
2	31.014236899158682	31.0	31.0	34.0	28.0	34.0
3	30.848694866287897	31.0	30.0	34.0	27.0	34.0
4	34.75244855371586	35.0	35.0	37.0	32.0	37.0
5	34.71151239128117	35.0	35.0	37.0	32.0	37.0
6	34.88374383736221	35.0	35.0	37.0	32.0	37.0
7	35.32137870456403	36.0	35.0	37.0	33.0	37.0
8	34.994826176803606	37.0	35.0	37.0	32.0	37.0
9	36.76245347874874	39.0	37.0	39.0	32.0	39.0
10	36.075858199672005	37.0	35.0	39.0	32.0	39.0
11	36.4298919031444	38.0	35.0	39.0	32.0	39.0
12	36.638786918706494	39.0	35.0	39.0	32.0	39.0
13	36.63438739191584	39.0	35.0	39.0	32.0	39.0
14	37.704698582910645	39.0	37.0	41.0	33.0	41.0
15	37.781445319441694	39.0	37.0	41.0	33.0	41.0
16	37.8932435657238	39.0	37.0	41.0	33.0	41.0
17	37.66326737851163	39.0	36.0	41.0	33.0	41.0
18	37.68228460597199	39.0	36.0	41.0	33.0	41.0
19	37.60411621046646	39.0	37.0	40.0	33.0	41.0
20	37.445037648576054	39.0	36.0	41.0	32.0	41.0
21	37.61453747848471	39.0	36.0	41.0	33.0	41.0
22	37.71530263565419	39.0	36.0	41.0	33.0	41.0
23	37.66215543809945	39.0	36.0	41.0	33.0	41.0
24	37.63498651962651	40.0	36.0	41.0	32.0	41.0
25	37.39527704579265	39.0	36.0	41.0	32.0	41.0
26	37.36475961269948	39.0	36.0	41.0	32.0	41.0
27	37.31534934730621	39.0	36.0	41.0	32.0	41.0
28	37.221948891359865	39.0	36.0	41.0	31.0	41.0
29	37.25336501601905	39.0	36.0	41.0	31.0	41.0
30	37.01738231963971	39.0	36.0	41.0	31.0	41.0
31	37.07710976730489	39.0	36.0	41.0	31.0	41.0
32	36.89232456474387	39.0	36.0	41.0	31.0	41.0
33	36.85159149644839	39.0	35.0	41.0	31.0	41.0
34	36.796598680903564	39.0	36.0	41.0	31.0	41.0
35	36.76997557792976	39.0	36.0	41.0	30.0	41.0
36	36.573857722400774	39.0	35.0	41.0	30.0	41.0
37	36.53620660766782	39.0	35.0	41.0	30.0	41.0
38	36.428064055891504	39.0	35.0	41.0	30.0	41.0
39	36.34542758932334	39.0	35.0	41.0	30.0	41.0
40	36.32576553797099	39.0	35.0	41.0	30.0	41.0
41	36.20175879524557	39.0	35.0	40.0	29.0	41.0
42	36.24938690956726	39.0	35.0	41.0	30.0	41.0
43	36.22195904606683	39.0	35.0	40.0	30.0	41.0
44	36.34277721080664	39.0	35.0	41.0	30.0	41.0
45	36.39013876407061	39.0	35.0	41.0	30.0	41.0
46	36.27090219493991	39.0	35.0	40.0	30.0	41.0
47	36.188291115139144	39.0	35.0	40.0	30.0	41.0
48	36.16707793229857	39.0	35.0	40.0	29.0	41.0
49	36.20762821586876	39.0	35.0	40.0	30.0	41.0
50	36.044533467375466	39.0	35.0	40.0	29.0	41.0
51	34.957119211182366	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	12.0
10	17.0
11	21.0
12	11.0
13	9.0
14	19.0
15	16.0
16	29.0
17	54.0
18	108.0
19	309.0
20	660.0
21	1276.0
22	2066.0
23	2597.0
24	3056.0
25	3498.0
26	3779.0
27	3963.0
28	4464.0
29	5236.0
30	6669.0
31	8707.0
32	11422.0
33	15667.0
34	24556.0
35	28684.0
36	33294.0
37	50927.0
38	89279.0
39	93480.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.799713637263714	19.47698181799719	22.210375064101587	9.512929480637512
2	30.165826364665683	31.762907901885225	22.399252613567704	15.672013119881392
3	28.748483140647767	31.573776484745093	24.748290201215518	14.92945017339162
4	25.63961960467726	22.39315978939138	34.27315146253167	17.694069143399695
5	30.746929470482804	26.35679578376567	24.125806664534178	18.770468081217345
6	21.392159550755764	39.955725477652024	24.837143887120277	13.81497108447193
7	71.91766563596391	5.110102410219697	18.013180809634786	4.959051144181607
8	71.57291333465345	11.970622432763147	10.421521885932176	6.034942346651231
9	66.72962584982204	6.257076561413129	11.341284468883439	15.672013119881392
10	40.06412697445583	23.35404893553284	20.92885104568095	15.652973044330373
11	29.30115306697537	23.51423943783542	26.23468543256513	20.949922062624076
12	21.990779526079827	20.64426538311171	32.133808573619085	25.23114651718938
13	24.130630150340437	22.899625799048504	36.030677369727805	16.939066680883254
14	20.242646722822197	30.94672332993151	28.0955354830848	20.715094464161503
15	17.267825318730864	25.304768142653323	36.83086827821866	20.59653826039715
16	19.337100729615695	25.365696384416587	28.602001492741923	26.695201393225794
17	19.503637923768615	28.15240184206384	29.727650759318212	22.61630947484933
18	19.830365620224114	25.17402629053632	31.109452508974222	23.886155580265342
19	18.908572095880743	27.701025117667665	28.111021411199626	25.279381375251965
20	22.515016272917904	27.01888267759313	31.92284453651379	18.543256512975177
21	20.222083441227095	31.505993815783462	29.721557935141885	18.550364807847558
22	18.92735830375775	23.384005321066446	31.96930232085828	25.719334054317528
23	20.053515305682065	28.793925454296204	29.375028560113325	21.777530679908406
24	21.482028707356577	26.320746574055736	27.914273963839086	24.282950754748594
25	17.78952338882881	33.69077901834448	26.02219818941575	22.497499403410966
26	18.005564779414378	25.904403588673443	31.250095200377753	24.839936431534426
27	23.169487136524957	28.496392540352268	26.488553106578728	21.845567216544048
28	17.50392225556351	27.364904317273663	31.479337710012032	23.651835717150792
29	19.68312236929623	23.38832107152468	30.301391702588944	26.627164856590152
30	19.437886196199095	32.86824775454043	27.599731915736243	20.094134133524243
31	23.455849872812294	25.469782130762162	24.242078059232405	26.83228993719314
32	23.265195249628086	28.265626824673905	27.440049148781686	21.02912877691632
33	21.247201108894	27.902088315486434	25.70410199387671	25.146608581742854
34	21.081425517763122	23.589892004691475	26.437017968753967	28.891664508791436
35	22.100704228927714	26.036922514508536	26.428894203185532	25.433479053378218
36	21.905226119937243	27.190497225226324	26.951607743979526	23.952668910856907
37	19.31222169756236	27.230100582372447	31.657806684843592	21.799871035221603
38	22.17305651602159	27.4720364757074	26.145324011312344	24.209582996958666
39	20.87960071692231	24.714779668245722	30.30418424700309	24.10143536782887
40	21.56478956908501	26.092265667443503	28.1254918686184	24.217452894853086
41	19.347763171924264	25.115890593187206	27.3301244459338	28.20622178895473
42	22.834889542175034	23.267987794042234	31.041162104664565	22.855960559118166
43	21.954476448695882	24.016389697034317	29.741359613714945	24.287774240554853
44	20.641218971023545	23.971962854081937	29.575837890258082	25.810980284636436
45	20.723725965077964	22.63738049179246	27.535249526536788	29.10364401659279
46	25.25932582900489	25.312638040547746	26.965824333724292	22.462211796723075
47	18.082486684640497	25.25374074017659	34.36124354541439	22.30252902976852
48	21.059846765471963	24.653597558808446	28.12701507466248	26.159540601057106
49	20.784146471493198	21.371088533812635	34.76870116220621	23.076063832487954
50	21.426939422095625	21.556919671190588	30.964494067112458	26.05164683960133
51	19.73618071316507	21.311937365767466	27.556320543479917	31.395561377587544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	221.5
2	325.0
3	1149.0
4	1973.0
5	1386.5
6	800.0
7	806.0
8	812.0
9	781.5
10	751.0
11	735.5
12	720.0
13	713.5
14	707.0
15	724.0
16	741.0
17	744.5
18	748.0
19	760.0
20	772.0
21	889.0
22	1006.0
23	1346.5
24	1687.0
25	1903.5
26	2389.5
27	2659.0
28	3256.0
29	3853.0
30	4572.5
31	5292.0
32	5977.0
33	6662.0
34	8222.0
35	9782.0
36	10700.0
37	11618.0
38	12542.0
39	13466.0
40	15370.5
41	17275.0
42	19338.5
43	21402.0
44	24667.0
45	27932.0
46	46259.5
47	64587.0
48	55750.5
49	46914.0
50	45837.0
51	44760.0
52	37135.5
53	29511.0
54	25832.0
55	22153.0
56	19012.5
57	15872.0
58	13719.0
59	11566.0
60	10212.5
61	8859.0
62	7438.0
63	6017.0
64	5059.5
65	4102.0
66	3521.0
67	2940.0
68	2193.0
69	1446.0
70	1101.5
71	757.0
72	630.0
73	503.0
74	394.5
75	268.0
76	250.0
77	166.0
78	82.0
79	66.5
80	51.0
81	31.5
82	12.0
83	11.0
84	10.0
85	5.5
86	1.0
87	2.5
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	393906.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.77838414908526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.45160571092683	31.104677839073307
2	10.338004179432508	8.638102198863606
3	3.7769564655209025	4.733854143927107
4	2.208423573070917	3.690574735986089
5	1.5684050617704872	3.27627145860086
6	1.2332795218830643	3.0914655377058518
7	0.9798669631059518	2.865608087974661
8	0.8464782003943186	2.829159314392018
9	0.6621939976106376	2.48988556920561
>10	3.8544229893454904	24.17558035727304
>50	0.046623004636294	1.3442616144577486
>100	0.025151884080105973	1.8655634493113349
>500	0.0024538423492786315	0.7717009954494339
>1k	0.004294224111237605	3.0604057079580507
>5k	0.0018403817619589734	6.062888989821259
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGC	8306	2.1086249003569377	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTC	8217	2.086030677369728	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCG	7133	1.8108381187389884	No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	3504	0.8895523297436444	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCT	1663	0.4221819418846121	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCT	1587	0.4028879986595787	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGTACGTT	1469	0.3729316131259742	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTA	1392	0.3533838022269272	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTC	1265	0.32114260762720037	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1061	0.2693536021284266	No Hit
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	907	0.23025798033033262	No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT	876	0.2223880824359111	Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTT	709	0.17999218087564037	No Hit
TCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	519	0.1317573228130569	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.077353480271943E-4	0.0	0.0	0.2320350540484278	0.0
2	5.077353480271943E-4	0.0	0.0	1.6336384822774976	0.0
3	7.616030220407914E-4	0.0	0.0	2.043888643483471	0.0
4	7.616030220407914E-4	0.0	0.0	2.9997004361446638	0.0
5	7.616030220407914E-4	0.0	0.0	6.5830426548465875	0.0
6	7.616030220407914E-4	0.0	0.0	7.611968337623697	0.0
7	7.616030220407914E-4	0.0	0.0	8.839164673805426	0.0
8	7.616030220407914E-4	0.0	0.0	10.432945931262788	0.0
9	7.616030220407914E-4	0.0	0.0	10.838372606662503	0.0
10	7.616030220407914E-4	0.0	0.0	13.776383198021863	0.0
11	7.616030220407914E-4	0.0	0.0	15.534162972892009	0.0
12	7.616030220407914E-4	0.0	0.0	19.038552344975706	0.0
13	7.616030220407914E-4	0.0	0.0	19.678552751163984	0.0
14	7.616030220407914E-4	0.0	0.0	19.984971033698397	0.0
15	7.616030220407914E-4	0.0	0.0	20.730834259950342	0.0
16	7.616030220407914E-4	0.0	0.0	21.667352109386503	0.0
17	7.616030220407914E-4	0.0	0.0	22.862307250968506	0.0
18	7.616030220407914E-4	0.0	0.0	24.023244124232686	0.0
19	7.616030220407914E-4	0.0	0.0	25.22124567790285	0.0
20	7.616030220407914E-4	0.0	0.0	25.984625773661737	0.0
21	7.616030220407914E-4	0.0	0.0	26.858692175290553	0.0
22	7.616030220407914E-4	0.0	0.0	27.860454016948207	0.0
23	7.616030220407914E-4	0.0	0.0	28.822866369133752	0.0
24	7.616030220407914E-4	0.0	0.0	29.565937050971552	0.0
25	7.616030220407914E-4	0.0	0.0	30.21710763481643	0.0
26	7.616030220407914E-4	0.0	0.0	30.827405523145117	0.0
27	7.616030220407914E-4	0.0	0.0	31.48543053418836	0.0
28	7.616030220407914E-4	0.0	0.0	32.11222982132793	0.0
29	7.616030220407914E-4	0.0	0.0	32.75705371332247	0.0
30	7.616030220407914E-4	0.0	0.0	33.42751824039238	0.0
31	7.616030220407914E-4	0.0	0.0	34.09772889978827	0.0
32	7.616030220407914E-4	0.0	0.0	34.70244169928866	0.0
33	7.616030220407914E-4	0.0	0.0	35.304361954374905	0.0
34	7.616030220407914E-4	0.0	0.0	35.90298192969897	0.0
35	7.616030220407914E-4	0.0	0.0	36.483323432494046	0.0
36	7.616030220407914E-4	0.0	0.0	37.04462485973811	0.0
37	7.616030220407914E-4	0.0	0.0	37.63029758368748	0.0
38	0.0010154706960543887	0.0	0.0	38.215970307636844	0.0
39	0.0010154706960543887	0.0	0.0	38.8181444303971	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATGC	60	0.0	45.000004	9
CTAGCGG	30	2.1618544E-6	45.000004	2
CGGCGAA	140	0.0	45.0	31
ACCGGGT	20	7.027634E-4	45.0	4
CCGATCG	40	6.7957444E-9	45.0	40
GGCCGAT	20	7.027634E-4	45.0	8
CCGGAGC	20	7.027634E-4	45.0	14
TACGGAG	20	7.027634E-4	45.0	29
CTAACGG	20	7.027634E-4	45.0	2
CGCTCGA	35	1.2092096E-7	45.0	41
TCTACGG	20	7.027634E-4	45.0	2
CGCAGAT	20	7.027634E-4	45.0	28
GTATACG	20	7.027634E-4	45.0	1
CTCGAAT	70	0.0	45.0	43
CGATCGA	90	0.0	45.0	41
TACGGCT	965	0.0	42.668392	7
CGATGAA	885	0.0	42.457626	19
ACGGCTG	980	0.0	42.015305	8
CCGATGA	870	0.0	41.896553	18
TTAGCGG	65	0.0	41.53846	2
>>END_MODULE