FastQCFastQC Report
Sat 14 Jan 2017
SRR2935537.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935537.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445469
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58931.3228754413887387No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC48331.0849239789974163No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC21360.47949464496968364No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG19390.4352715901667681No Hit
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC16960.38072233982611586Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT16200.36366166893768137No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTT14810.3324585998127816No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC12220.2743176292850905No Hit
CGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG11250.25254282565116765No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT8870.19911598786896506No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTT7630.17128015641941413No Hit
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC7340.16477016358040628Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT6940.1557908631128092No Hit
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT5890.1322201993853669Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT5650.12683261910480864No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTC5510.12368986394114966No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC5470.12279193389438994No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGC253.886946E-545.04
GCGCGAC351.2096825E-745.09
GTAATCG207.02863E-445.01
AATTGCG207.02863E-445.01
TCGAGAG207.02863E-445.01
ATCGGGC207.02863E-445.04
GCCAACG253.886946E-545.01
TATCCGG207.02863E-445.02
CTAACGG302.1624965E-644.9999962
CGTTTTT33650.044.0638921
CGATGAA3100.042.09677519
TAGACGG650.041.5384642
CCGATCG501.0786607E-940.540
TACGAAT501.0786607E-940.512
ACGGGTC451.9237632E-840.05
TGACGGG1750.039.8571433
TACGGGA2050.039.5121964
CCGATGA3250.039.4615418
CGCGACC403.4518598E-739.37510
GTTACGG800.039.3752
Produced by FastQC (version 0.11.5)