##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935537.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445469
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.116156230848837	31.0	31.0	33.0	28.0	34.0
2	31.1658319658607	31.0	31.0	34.0	28.0	34.0
3	30.907261784770657	31.0	30.0	34.0	27.0	34.0
4	34.6834796585172	35.0	35.0	37.0	32.0	37.0
5	34.7437868852827	35.0	35.0	37.0	32.0	37.0
6	34.951525246425675	36.0	35.0	37.0	32.0	37.0
7	35.308876711959755	37.0	35.0	37.0	33.0	37.0
8	35.15689531707032	37.0	35.0	37.0	32.0	37.0
9	37.11572971407662	39.0	37.0	39.0	34.0	39.0
10	36.445797575139906	38.0	35.0	39.0	32.0	39.0
11	36.68643160354593	39.0	35.0	39.0	32.0	39.0
12	36.91422074263305	39.0	37.0	39.0	33.0	39.0
13	37.02727462517033	39.0	37.0	39.0	33.0	39.0
14	38.14088746916171	40.0	37.0	41.0	33.0	41.0
15	38.273671119651425	40.0	38.0	41.0	34.0	41.0
16	38.25177285063607	40.0	37.0	41.0	34.0	41.0
17	38.1039174443115	40.0	37.0	41.0	33.0	41.0
18	37.99024847969219	39.0	37.0	41.0	33.0	41.0
19	37.78537451539838	39.0	37.0	41.0	33.0	41.0
20	37.709676767631414	39.0	36.0	41.0	33.0	41.0
21	37.69979729229195	39.0	36.0	41.0	33.0	41.0
22	37.80182010420478	39.0	36.0	41.0	33.0	41.0
23	37.83464169223897	40.0	36.0	41.0	33.0	41.0
24	37.80860621053317	40.0	36.0	41.0	33.0	41.0
25	37.424000323254816	39.0	35.0	41.0	33.0	41.0
26	37.483714916189456	39.0	35.0	41.0	33.0	41.0
27	37.55205861687345	39.0	35.0	41.0	33.0	41.0
28	37.4365376715327	39.0	35.0	41.0	33.0	41.0
29	37.4000076324054	40.0	35.0	41.0	33.0	41.0
30	37.08406645580276	39.0	35.0	41.0	32.0	41.0
31	36.99576177017929	39.0	35.0	41.0	31.0	41.0
32	36.81992686359769	39.0	35.0	41.0	31.0	41.0
33	36.60808271731591	40.0	35.0	41.0	30.0	41.0
34	36.35661291807062	40.0	35.0	41.0	29.0	41.0
35	36.28826023808615	40.0	35.0	41.0	29.0	41.0
36	36.10318787614851	40.0	35.0	41.0	27.0	41.0
37	36.120854649818504	40.0	35.0	41.0	27.0	41.0
38	35.95624835847163	39.0	35.0	41.0	27.0	41.0
39	35.84482421896922	39.0	35.0	41.0	26.0	41.0
40	35.74995790952906	39.0	35.0	41.0	25.0	41.0
41	35.564212100056345	39.0	35.0	41.0	24.0	41.0
42	35.618415647328995	39.0	35.0	41.0	25.0	41.0
43	35.610754059205014	39.0	35.0	41.0	24.0	41.0
44	35.63212928396813	39.0	35.0	41.0	24.0	41.0
45	35.62965324186419	39.0	35.0	41.0	24.0	41.0
46	35.51143850638316	39.0	35.0	41.0	24.0	41.0
47	35.45345018396342	39.0	35.0	41.0	24.0	41.0
48	35.397524855826106	39.0	35.0	41.0	24.0	41.0
49	35.35878590878377	39.0	35.0	41.0	24.0	41.0
50	35.26040420321055	39.0	35.0	41.0	24.0	41.0
51	34.16822494943531	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	24.0
10	28.0
11	20.0
12	22.0
13	24.0
14	23.0
15	29.0
16	46.0
17	105.0
18	185.0
19	352.0
20	690.0
21	1179.0
22	1685.0
23	2642.0
24	3828.0
25	5829.0
26	7512.0
27	7778.0
28	7497.0
29	7076.0
30	7564.0
31	9097.0
32	11338.0
33	15364.0
34	24292.0
35	29663.0
36	34557.0
37	53016.0
38	96085.0
39	117889.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.70900556492146	19.74368586815244	21.76874260610727	17.778565960818824
2	37.29844276481641	24.58756950539768	22.90552204530506	15.208465684480851
3	28.82400346600998	24.545591275711665	31.626667624458715	15.003737633819636
4	25.26550669070126	23.112943886106553	33.881145489360655	17.740403933831537
5	24.474430319505956	27.345337161508432	29.721933512769688	18.45829900621592
6	22.706181574924404	32.62651273152565	30.08222794403202	14.585077749517925
7	72.75814927638062	4.9974296752411504	17.515023492094848	4.729397556283377
8	73.39006754678776	6.0648440183267525	15.276708368034589	5.2683800668508916
9	67.8271664245997	6.992854721652909	16.813291160552136	8.366687693195262
10	33.11880287966166	30.07616691621639	23.269857161777814	13.535173042344137
11	23.88269441869131	23.992915331931066	34.014488101304465	18.109902148073154
12	21.706560950369163	21.027725835018824	37.18036496366751	20.08534825094451
13	21.58960556177871	22.310643389326753	38.54791242488254	17.551838624011996
14	17.637366460965858	27.636042014146888	34.90613263773686	19.820458887150398
15	16.248942126163662	26.347287914535016	38.980490224909026	18.423279734392292
16	19.494959245200004	25.964545232103692	35.100983457883714	19.439512064812593
17	19.22849850382406	26.28218798614494	33.77878146403005	20.710532046000957
18	19.984780085707424	26.11831575261129	33.43554770365615	20.461356458025136
19	19.56499778884726	27.832688694387265	31.82600809483937	20.776305421926107
20	21.613625190529532	26.723520604127337	33.19939210135834	18.46346210398479
21	21.16376223710292	27.559044512637243	33.398283606715616	17.87890964354422
22	19.63346495491269	23.923550235818876	34.24256233318143	22.200422476087
23	19.272497076115286	26.62811553665912	34.14670830068983	19.952679086535763
24	20.380542753816762	24.78264480805623	33.97542814427042	20.861384293856585
25	19.851661956275297	28.081639799851395	31.31329003813958	20.75340820573373
26	18.877183373029325	26.48601810675939	32.31515548781172	22.32164303239956
27	21.167578439801645	25.8172847044351	31.621729009201538	21.39340784656171
28	18.160859678226767	25.791918180614136	33.74802735992852	22.299194781230568
29	19.06283041019689	23.818716902859684	32.44557982710357	24.672872859839853
30	19.245110209689116	24.31639463127625	32.72999916941471	23.70849598961993
31	19.149031694685824	25.14406165187701	31.568975618954404	24.137931034482758
32	20.124408207978558	25.50794780332638	30.975892823069618	23.39175116562544
33	19.558263313496564	25.329484206532886	31.85384392628892	23.258408553681626
34	19.14005239421823	26.696358220212858	31.65315656083813	22.51043282473079
35	20.416684438198843	25.890241520734325	29.34704771824751	24.34602632281932
36	19.43771620471907	29.000671202709956	29.999842862241817	21.561769730329157
37	18.995036691666538	28.192758643137907	30.64141388065163	22.170790784543932
38	19.865579872000072	26.879535949751837	30.362831083644426	22.892053094603664
39	20.906280796194572	26.265800762791574	30.126226516323246	22.701691924690607
40	21.31394103742348	25.439031672237572	32.34590959191324	20.901117698425704
41	19.065075235313795	28.500299684153106	30.26001809329044	22.174606987242658
42	21.240984221124254	27.14150704089398	29.93586534641019	21.68164339157158
43	20.778774729554694	26.576035593947054	30.14238925716492	22.502800419333333
44	20.474151961191463	25.403787917902253	29.927559493477663	24.19450062742862
45	20.19983433190637	24.211785780828745	29.591733655989533	25.99664623127535
46	21.61631898066981	25.699431385797887	29.33492566261625	23.349323970916046
47	19.47744960928819	25.47764266424824	32.38541851397067	22.6594892124929
48	19.415267953550078	25.190080566773442	31.06186962504686	24.332781854629616
49	19.768603426950023	24.327169791837367	32.93203342993564	22.972193351276967
50	18.69961770628259	24.042974932037918	33.106231859007025	24.151175502672466
51	18.4921958654811	23.61892746745565	31.0075448572179	26.881331809845356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144.0
1	278.0
2	412.0
3	5211.5
4	10011.0
5	6829.5
6	3648.0
7	3477.5
8	3307.0
9	3312.0
10	3317.0
11	3204.0
12	3091.0
13	2935.5
14	2780.0
15	2702.0
16	2624.0
17	2540.5
18	2457.0
19	2360.5
20	2264.0
21	2281.0
22	2298.0
23	2495.5
24	2693.0
25	2859.0
26	3755.0
27	4485.0
28	5142.5
29	5800.0
30	6933.0
31	8066.0
32	9196.5
33	10327.0
34	11717.5
35	13108.0
36	14470.0
37	15832.0
38	16894.0
39	17956.0
40	19698.0
41	21440.0
42	22907.0
43	24374.0
44	26564.0
45	28754.0
46	39878.0
47	51002.0
48	47383.5
49	43765.0
50	42066.5
51	40368.0
52	34862.0
53	29356.0
54	25297.5
55	21239.0
56	18777.0
57	16315.0
58	15405.0
59	14495.0
60	13463.0
61	12431.0
62	11177.0
63	9923.0
64	7999.5
65	6076.0
66	4738.0
67	3400.0
68	2685.0
69	1970.0
70	1621.5
71	1273.0
72	1014.0
73	755.0
74	599.5
75	345.5
76	247.0
77	174.0
78	101.0
79	69.0
80	37.0
81	31.0
82	25.0
83	25.5
84	26.0
85	16.5
86	7.0
87	3.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	445469.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.93568571525791
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78151408225172	33.15422928395939
2	9.5276701217543	8.562647803796084
3	4.135271840754623	5.574638273499174
4	2.747534261160171	4.938493442055872
5	1.9655949387641198	4.416267820590305
6	1.5502810020861184	4.179776392804615
7	1.2754761574829236	4.012007702497022
8	1.0138997486372328	3.6448224361233352
9	0.8108532025953208	3.2792620205730314
>10	3.1353525032926197	19.94579830207134
>50	0.036354947927701824	1.1228968348805113
>100	0.011613386143563916	0.9806344959287082
>500	0.004039438658630927	1.2093433279268893
>1k	0.004039438658630927	3.6420973920980155
>5k	5.049298323288659E-4	1.3370844711957144
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5893	1.3228754413887387	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC	4833	1.0849239789974163	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	2136	0.47949464496968364	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	1939	0.4352715901667681	No Hit
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1696	0.38072233982611586	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	1620	0.36366166893768137	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTT	1481	0.3324585998127816	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC	1222	0.2743176292850905	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	1125	0.25254282565116765	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT	887	0.19911598786896506	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTT	763	0.17128015641941413	No Hit
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	734	0.16477016358040628	Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	694	0.1557908631128092	No Hit
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT	589	0.1322201993853669	Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT	565	0.12683261910480864	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTC	551	0.12368986394114966	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC	547	0.12279193389438994	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.734475350697803E-4	0.0	0.0	0.13962812227113447	0.0
2	6.734475350697803E-4	0.0	0.0	0.8108308322240156	0.0
3	6.734475350697803E-4	0.0	0.0	1.2698975686299159	0.0
4	6.734475350697803E-4	0.0	0.0	2.1447059166855604	0.0
5	6.734475350697803E-4	0.0	0.0	4.44767200411252	0.0
6	6.734475350697803E-4	0.0	0.0	5.9822344540248595	0.0
7	6.734475350697803E-4	0.0	0.0	7.108463215173222	0.0
8	6.734475350697803E-4	0.0	0.0	8.541110604778336	0.0
9	6.734475350697803E-4	0.0	0.0	9.16786577741661	0.0
10	6.734475350697803E-4	0.0	0.0	10.547984259286281	0.0
11	6.734475350697803E-4	0.0	0.0	11.757271549759915	0.0
12	8.979300467597072E-4	0.0	0.0	13.21483649816261	0.0
13	8.979300467597072E-4	0.0	0.0	13.751349701101535	0.0
14	8.979300467597072E-4	0.0	0.0	14.046993168997169	0.0
15	0.001122412558449634	0.0	0.0	14.433552054127222	0.0
16	0.001122412558449634	0.0	0.0	14.968269396972628	0.0
17	0.001122412558449634	0.0	0.0	15.657879672884084	0.0
18	0.001122412558449634	0.0	0.0	16.37981543047889	0.0
19	0.001122412558449634	0.0	0.0	16.935409646911456	0.0
20	0.001122412558449634	0.0	0.0	17.41647566946297	0.0
21	0.001122412558449634	0.0	0.0	17.958151970170764	0.0
22	0.001122412558449634	0.0	0.0	18.58782541546101	0.0
23	0.001122412558449634	0.0	0.0	19.168112708179468	0.0
24	0.001122412558449634	0.0	0.0	19.616628766535943	0.0
25	0.001122412558449634	0.0	0.0	20.0498800140975	0.0
26	0.001122412558449634	0.0	0.0	20.44541819969515	0.0
27	0.001122412558449634	0.0	0.0	20.87844496474502	0.0
28	0.001122412558449634	0.0	0.0	21.31775724012221	0.0
29	0.001122412558449634	0.0	0.0	21.76919157113065	0.0
30	0.001122412558449634	0.0	0.0	22.25497172642765	0.0
31	0.001122412558449634	0.0	0.0	22.72571155344143	0.0
32	0.001122412558449634	0.0	0.0	23.168391066493964	0.0
33	0.001122412558449634	0.0	0.0	23.62117229257255	0.0
34	0.001122412558449634	0.0	0.0	24.046791134736647	0.0
35	0.001122412558449634	0.0	0.0	24.49261340295284	0.0
36	0.001122412558449634	0.0	0.0	24.912395699813008	0.0
37	0.001122412558449634	0.0	0.0	25.35889141556427	0.0
38	0.001122412558449634	0.0	0.0	25.81638677438834	0.0
39	0.001122412558449634	0.0	0.0	26.286228671355357	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGC	25	3.886946E-5	45.0	4
GCGCGAC	35	1.2096825E-7	45.0	9
GTAATCG	20	7.02863E-4	45.0	1
AATTGCG	20	7.02863E-4	45.0	1
TCGAGAG	20	7.02863E-4	45.0	1
ATCGGGC	20	7.02863E-4	45.0	4
GCCAACG	25	3.886946E-5	45.0	1
TATCCGG	20	7.02863E-4	45.0	2
CTAACGG	30	2.1624965E-6	44.999996	2
CGTTTTT	3365	0.0	44.063892	1
CGATGAA	310	0.0	42.096775	19
TAGACGG	65	0.0	41.538464	2
CCGATCG	50	1.0786607E-9	40.5	40
TACGAAT	50	1.0786607E-9	40.5	12
ACGGGTC	45	1.9237632E-8	40.0	5
TGACGGG	175	0.0	39.857143	3
TACGGGA	205	0.0	39.512196	4
CCGATGA	325	0.0	39.46154	18
CGCGACC	40	3.4518598E-7	39.375	10
GTTACGG	80	0.0	39.375	2
>>END_MODULE