SRR2935535.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935535.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	456406
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2953	0.6470116519064166	No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	1105	0.24210899944347797	TruSeq Adapter, Index 27 (95% over 23bp)
GTGAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	816	0.1787881842044145	No Hit
CGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG	741	0.16235544668562638	No Hit
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	663	0.14526539966608676	TruSeq Adapter, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	521	0.11415274996384797	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTT	516	0.1130572341292621	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATGCA	20	7.028811E-4	45.000004	10
TGCGTTG	20	7.028811E-4	45.000004	1
TTAAGCG	25	3.8870967E-5	45.0	1
CGTTTTT	1525	0.0	43.52459	1
CACAACG	75	0.0	42.0	12
GCGATAA	35	6.240012E-6	38.57143	9
CTCGAAC	35	6.240012E-6	38.57143	12
GTACGAG	35	6.240012E-6	38.57143	1
CTAAGCC	30	1.13884365E-4	37.500004	17
GTTCGCG	30	1.13884365E-4	37.500004	1
TCTCGCG	30	1.13884365E-4	37.500004	32
CTCGCGC	30	1.13884365E-4	37.500004	33
ATGCGCG	30	1.13884365E-4	37.500004	1
ACGTAGG	60	1.546141E-10	37.500004	2
TCGCGCA	30	1.13884365E-4	37.500004	34
CTTGACC	200	0.0	37.125	7
CACGGGC	80	0.0	36.562504	4
AACGAGC	80	0.0	36.562504	15
GCGATGC	80	0.0	36.562504	9
TAGGGCG	155	0.0	36.29032	5