##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935535.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	456406
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.162765169607763	31.0	31.0	33.0	28.0	34.0
2	31.19575772448215	31.0	31.0	34.0	28.0	34.0
3	30.98746072575733	31.0	31.0	34.0	27.0	34.0
4	34.787693413320596	35.0	35.0	37.0	32.0	37.0
5	34.826100007449504	35.0	35.0	37.0	33.0	37.0
6	35.02079289054044	36.0	35.0	37.0	32.0	37.0
7	35.49285285469516	37.0	35.0	37.0	33.0	37.0
8	35.30839428053093	37.0	35.0	37.0	33.0	37.0
9	37.27622774459582	39.0	37.0	39.0	34.0	39.0
10	36.42761707777724	38.0	35.0	39.0	32.0	39.0
11	36.67305644535786	39.0	35.0	39.0	32.0	39.0
12	36.82859997458403	39.0	37.0	39.0	33.0	39.0
13	36.93929089451059	39.0	37.0	39.0	33.0	39.0
14	37.98595548700061	40.0	37.0	41.0	33.0	41.0
15	38.179993251622456	40.0	37.0	41.0	33.0	41.0
16	38.092713505081	40.0	37.0	41.0	33.0	41.0
17	38.026415077803534	40.0	37.0	41.0	33.0	41.0
18	37.98290337988545	39.0	37.0	41.0	33.0	41.0
19	37.8116019508946	39.0	37.0	41.0	33.0	41.0
20	37.91508437662958	39.0	36.0	41.0	34.0	41.0
21	37.82415875339062	39.0	36.0	41.0	33.0	41.0
22	37.92933265557421	40.0	36.0	41.0	33.0	41.0
23	37.97418088281048	40.0	36.0	41.0	34.0	41.0
24	37.95991069354916	40.0	36.0	41.0	34.0	41.0
25	37.4998575829415	39.0	36.0	41.0	33.0	41.0
26	37.64132592472492	39.0	36.0	41.0	33.0	41.0
27	37.72393220071603	40.0	36.0	41.0	33.0	41.0
28	37.61118390205212	40.0	36.0	41.0	33.0	41.0
29	37.60133302366752	40.0	36.0	41.0	33.0	41.0
30	37.31069048172022	39.0	36.0	41.0	32.0	41.0
31	37.27605465309396	39.0	36.0	41.0	32.0	41.0
32	37.23064113968703	39.0	36.0	41.0	32.0	41.0
33	37.17872464428601	40.0	36.0	41.0	32.0	41.0
34	37.073894734074486	40.0	35.0	41.0	32.0	41.0
35	37.082498915439324	40.0	36.0	41.0	32.0	41.0
36	36.93050704854888	40.0	35.0	41.0	31.0	41.0
37	36.93007760634172	40.0	35.0	41.0	31.0	41.0
38	36.77655859037787	39.0	35.0	41.0	31.0	41.0
39	36.690216605390816	39.0	35.0	41.0	31.0	41.0
40	36.62863546929707	39.0	35.0	41.0	31.0	41.0
41	36.47566202021884	39.0	35.0	41.0	30.0	41.0
42	36.4777259720512	39.0	35.0	41.0	30.0	41.0
43	36.437318966008334	39.0	35.0	41.0	30.0	41.0
44	36.40569142386384	39.0	35.0	41.0	30.0	41.0
45	36.39187258712637	39.0	35.0	41.0	30.0	41.0
46	36.269389534756336	39.0	35.0	41.0	30.0	41.0
47	36.27597139389053	39.0	35.0	41.0	30.0	41.0
48	36.18100769928529	39.0	35.0	41.0	30.0	41.0
49	36.15189984356034	39.0	35.0	40.0	30.0	41.0
50	36.05125918590027	39.0	35.0	40.0	30.0	41.0
51	34.929253778434116	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	22.0
10	34.0
11	20.0
12	16.0
13	18.0
14	15.0
15	28.0
16	55.0
17	80.0
18	174.0
19	287.0
20	477.0
21	700.0
22	1109.0
23	1762.0
24	2509.0
25	3685.0
26	4611.0
27	5227.0
28	5347.0
29	6026.0
30	6916.0
31	8873.0
32	11700.0
33	16901.0
34	29099.0
35	32761.0
36	38000.0
37	58136.0
38	102474.0
39	119313.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17626411572153	20.85511583984435	23.68329951841124	13.285320526022884
2	34.617862166579755	22.296814678159357	25.257336669544223	17.827986485716664
3	32.431650767080185	21.854883590487418	28.99195014964746	16.72151549278493
4	27.54521193849336	25.844971363216086	27.07983681196128	19.529979886329276
5	24.15108477977941	30.567521022948867	26.215474818473027	19.0659193787987
6	24.769393916819674	33.62422930461037	26.653681152307374	14.952695626262583
7	79.0625013693948	5.5829677962165265	10.383956389705656	4.970574444683024
8	80.53377913524362	4.818955053176339	10.057492670999068	4.589773140580974
9	74.96658676704513	6.941845637436843	12.637870667782632	5.453696927735393
10	38.094372116054565	28.671840422781468	18.830602577529657	14.403184883634307
11	26.905430691095212	26.30377339474065	27.392935237485922	19.39786067667822
12	24.934816807842143	23.099827784910804	31.630171382497164	20.335184024749893
13	23.795042133539	24.312344710630448	32.52608423202149	19.366528923809064
14	20.5100283519498	27.013229449218457	31.535957020722776	20.940785178108964
15	20.0214282897245	26.183047549769285	34.16475681739504	19.630767343111177
16	23.17914313133482	25.737829914593586	31.34248892433491	19.740538029736683
17	23.058636389530374	25.02464910627818	29.144226850654896	22.772487653536544
18	24.01984198279602	25.49396808981477	30.007493328308566	20.478696599080642
19	22.944702742733444	27.079398605627446	29.396852802110402	20.57904584952871
20	24.47470015731607	26.250531325179775	29.287958528152565	19.98680998935159
21	24.520492719201766	26.169682256587336	30.060954501036356	19.248870523174542
22	23.505168643707577	24.4291266985973	29.658681086576426	22.407023571118696
23	21.5395941332936	26.32546460826545	30.113977467430313	22.020963791010637
24	21.85926565382576	25.167066164774344	31.09818889322226	21.875479288177633
25	21.792877394249857	27.130668746686066	28.806150664101697	22.27030319496238
26	21.011336397856294	27.01169572705004	29.25049188660973	22.726475988483937
27	21.715533976328093	26.464376016090934	29.29299790099166	22.527092106589308
28	19.849651406861433	26.504910101970612	30.54144774608572	23.10399074508223
29	20.560860286674583	25.685902464034214	29.878879769328186	23.874357479963017
30	21.599190194695076	25.00536802758947	29.99347072562587	23.401971052089586
31	22.04769437737453	26.212626477303104	28.448574295692868	23.291104849629495
32	21.663387422601808	26.657625009311886	28.27022431782229	23.40876325026402
33	21.53039180028308	25.513687374837318	28.695722667975442	24.26019815690416
34	20.728255106199306	25.6499695446598	30.192416401186662	23.429358947954235
35	20.650035275609874	25.15589190326157	29.95227933024544	24.24179349088312
36	22.439669942989358	26.560124100033743	28.622104003891273	22.37810195308563
37	21.153972559519374	27.268046432343134	28.826527258624996	22.751453749512496
38	21.04420187289387	27.50971722545278	28.53336722128982	22.912713680363535
39	21.863428613997186	24.770927638988095	29.62734933370727	23.73829441330745
40	22.43178222898034	23.79044096703374	31.44590561911982	22.331871184866106
41	20.592411142710656	25.03648067729171	29.968712067764226	24.402396112233408
42	21.88950189086033	25.178897735787874	29.168766405349622	23.762833968002173
43	22.093267836093304	25.45518682927043	28.601289203034142	23.850256131602126
44	21.44757080318839	25.428018036572702	28.48012515172894	24.644286008509965
45	21.205023597411078	24.670140182206193	28.649272796589003	25.47556342379373
46	21.578813600171777	25.05378982747817	29.43979702282617	23.92759954952389
47	20.531281359140767	25.140773784744287	30.723303374626976	23.604641481487974
48	20.514410415288143	24.52925684587845	30.49061581136094	24.46571692747247
49	21.443407843016963	24.45322804695819	30.44526145580908	23.658102654215764
50	20.265947424004064	24.128078947253105	31.08000333036814	24.52597029837469
51	20.215334592446197	23.43133087645649	29.907801387361253	26.44553314373606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	182.0
1	355.5
2	529.0
3	2703.0
4	4877.0
5	3255.5
6	1634.0
7	1648.0
8	1662.0
9	1624.0
10	1586.0
11	1583.0
12	1580.0
13	1540.0
14	1500.0
15	1462.0
16	1424.0
17	1412.5
18	1401.0
19	1365.0
20	1329.0
21	1359.5
22	1390.0
23	1601.0
24	1812.0
25	2151.5
26	3225.5
27	3960.0
28	4628.0
29	5296.0
30	6661.0
31	8026.0
32	8802.0
33	9578.0
34	11204.0
35	12830.0
36	14051.0
37	15272.0
38	17006.5
39	18741.0
40	20139.0
41	21537.0
42	22948.5
43	24360.0
44	27777.5
45	31195.0
46	34911.0
47	38627.0
48	41801.0
49	44975.0
50	43707.0
51	42439.0
52	37351.5
53	32264.0
54	28988.0
55	25712.0
56	23509.0
57	21306.0
58	19987.5
59	18669.0
60	17588.5
61	16508.0
62	14775.0
63	13042.0
64	11603.0
65	10164.0
66	8561.0
67	6958.0
68	5719.5
69	4481.0
70	3797.5
71	3114.0
72	2397.5
73	1681.0
74	1359.0
75	870.5
76	704.0
77	494.5
78	285.0
79	207.5
80	130.0
81	97.5
82	65.0
83	44.0
84	23.0
85	19.0
86	15.0
87	10.0
88	5.0
89	4.0
90	3.0
91	3.0
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	456406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.3783883409478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.44449128215629	28.18681207929949
2	8.896634761212617	6.828770075867864
3	3.43666335036792	3.956808019725683
4	2.0431166458613172	3.1364609624288144
5	1.5491293013756007	2.972654295926698
6	1.2728334019049483	2.930957675498268
7	1.0911367855808227	2.931324990308001
8	1.0118515972949655	3.106658683551561
9	0.8363563651611157	2.888820843621916
>10	6.343950169657813	38.525009765854925
>50	0.04557086573983664	1.2176139371115873
>100	0.024227548874343512	1.6986821344467644
>500	0.002884232008850418	0.7210488351222615
>1k	0.0011536928035401671	0.8983777012361489
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2953	0.6470116519064166	No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	1105	0.24210899944347797	TruSeq Adapter, Index 27 (95% over 23bp)
GTGAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	816	0.1787881842044145	No Hit
CGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG	741	0.16235544668562638	No Hit
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	663	0.14526539966608676	TruSeq Adapter, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	521	0.11415274996384797	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTT	516	0.1130572341292621	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11765840063452278	0.0
2	0.0	0.0	0.0	0.6244440257139476	0.0
3	0.0	0.0	0.0	0.9493740222521176	0.0
4	0.0	0.0	0.0	1.3610688728894886	0.0
5	0.0	0.0	0.0	2.378803083219765	0.0
6	0.0	0.0	0.0	3.211176014338111	0.0
7	0.0	0.0	0.0	3.9504300995166584	0.0
8	0.0	0.0	0.0	4.944282064652962	0.0
9	0.0	0.0	0.0	5.309746147070809	0.0
10	0.0	0.0	0.0	6.135326880014723	0.0
11	0.0	0.0	0.0	7.258230610465244	0.0
12	0.0	0.0	0.0	8.30773477999851	0.0
13	0.0	0.0	0.0	8.727317344644899	0.0
14	0.0	0.0	0.0	8.946201408395156	0.0
15	0.0	0.0	0.0	9.23607489822658	0.0
16	0.0	0.0	0.0	9.819327528560098	0.0
17	0.0	0.0	0.0	10.564716502412326	0.0
18	0.0	0.0	0.0	11.353926109647988	0.0
19	0.0	0.0	0.0	11.830913704026678	0.0
20	0.0	0.0	0.0	12.272844791698619	0.0
21	0.0	0.0	0.0	12.8403219940141	0.0
22	0.0	0.0	0.0	13.457316512052865	0.0
23	2.1910316691717461E-4	0.0	0.0	14.074530133258547	0.0
24	2.1910316691717461E-4	0.0	0.0	14.554585171974075	0.0
25	2.1910316691717461E-4	0.0	0.0	15.030039044184345	0.0
26	2.1910316691717461E-4	0.0	0.0	15.431436045976609	0.0
27	2.1910316691717461E-4	0.0	0.0	15.845760134616986	0.0
28	2.1910316691717461E-4	0.0	0.0	16.28900584129043	0.0
29	2.1910316691717461E-4	0.0	0.0	16.757667515326265	0.0
30	2.1910316691717461E-4	0.0	0.0	17.288335385599662	0.0
31	2.1910316691717461E-4	0.0	0.0	17.76094091664001	0.0
32	2.1910316691717461E-4	0.0	0.0	18.237928511018698	0.0
33	2.1910316691717461E-4	0.0	0.0	18.67438201951771	0.0
34	4.3820633383434923E-4	0.0	0.0	19.155751677234743	0.0
35	4.3820633383434923E-4	0.0	0.0	19.612362677090136	0.0
36	6.573095007515238E-4	0.0	0.0	20.035012686073365	0.0
37	6.573095007515238E-4	0.0	0.0	20.497101265101687	0.0
38	6.573095007515238E-4	0.0	0.0	20.94670096361573	0.0
39	6.573095007515238E-4	0.0	0.0	21.450857350692146	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATGCA	20	7.028811E-4	45.000004	10
TGCGTTG	20	7.028811E-4	45.000004	1
TTAAGCG	25	3.8870967E-5	45.0	1
CGTTTTT	1525	0.0	43.52459	1
CACAACG	75	0.0	42.0	12
GCGATAA	35	6.240012E-6	38.57143	9
CTCGAAC	35	6.240012E-6	38.57143	12
GTACGAG	35	6.240012E-6	38.57143	1
CTAAGCC	30	1.13884365E-4	37.500004	17
GTTCGCG	30	1.13884365E-4	37.500004	1
TCTCGCG	30	1.13884365E-4	37.500004	32
CTCGCGC	30	1.13884365E-4	37.500004	33
ATGCGCG	30	1.13884365E-4	37.500004	1
ACGTAGG	60	1.546141E-10	37.500004	2
TCGCGCA	30	1.13884365E-4	37.500004	34
CTTGACC	200	0.0	37.125	7
CACGGGC	80	0.0	36.562504	4
AACGAGC	80	0.0	36.562504	15
GCGATGC	80	0.0	36.562504	9
TAGGGCG	155	0.0	36.29032	5
>>END_MODULE