##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935534.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513888
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.264989647549662	31.0	31.0	33.0	28.0	34.0
2	31.324994940531788	31.0	31.0	34.0	28.0	34.0
3	31.06358000186811	31.0	31.0	34.0	27.0	34.0
4	34.8051482034996	35.0	35.0	37.0	32.0	37.0
5	34.878969736596304	35.0	35.0	37.0	33.0	37.0
6	35.096240815119245	37.0	35.0	37.0	32.0	37.0
7	35.4750879569089	37.0	35.0	37.0	33.0	37.0
8	35.326425213276046	37.0	35.0	37.0	33.0	37.0
9	37.27370944641634	39.0	37.0	39.0	34.0	39.0
10	36.63553342362538	38.0	35.0	39.0	32.0	39.0
11	36.847750871785294	39.0	37.0	39.0	33.0	39.0
12	36.991161498225296	39.0	37.0	39.0	33.0	39.0
13	37.077139376673514	39.0	37.0	39.0	33.0	39.0
14	38.167616289930876	40.0	38.0	41.0	33.0	41.0
15	38.350553038794445	40.0	38.0	41.0	34.0	41.0
16	38.28421951864998	40.0	38.0	41.0	34.0	41.0
17	38.19685417834236	40.0	37.0	41.0	34.0	41.0
18	38.08090673454138	39.0	37.0	41.0	34.0	41.0
19	37.884815368329285	39.0	37.0	41.0	34.0	41.0
20	37.91117519770845	39.0	36.0	41.0	34.0	41.0
21	37.801264088673015	39.0	36.0	41.0	33.0	41.0
22	37.899855610561055	40.0	36.0	41.0	34.0	41.0
23	37.97155022105984	40.0	36.0	41.0	34.0	41.0
24	37.96687021296469	40.0	36.0	41.0	34.0	41.0
25	37.535887197210286	39.0	35.0	41.0	33.0	41.0
26	37.65594448595803	39.0	35.0	41.0	33.0	41.0
27	37.72113184195778	40.0	36.0	41.0	33.0	41.0
28	37.591891618407125	40.0	35.0	41.0	33.0	41.0
29	37.47986915436827	40.0	35.0	41.0	33.0	41.0
30	37.180796204620464	39.0	35.0	41.0	32.0	41.0
31	37.10237639329971	39.0	35.0	41.0	32.0	41.0
32	36.98891392677004	39.0	35.0	41.0	32.0	41.0
33	36.82168877265085	40.0	35.0	41.0	31.0	41.0
34	36.57082866305498	40.0	35.0	41.0	30.0	41.0
35	36.51344261784669	40.0	35.0	41.0	30.0	41.0
36	36.344182000747246	39.0	35.0	41.0	30.0	41.0
37	36.299294787969366	39.0	35.0	41.0	30.0	41.0
38	36.112898141229216	39.0	35.0	41.0	29.0	41.0
39	36.01505191792764	39.0	35.0	41.0	29.0	41.0
40	35.919449374182705	39.0	35.0	41.0	28.0	41.0
41	35.75081146086307	39.0	35.0	41.0	27.0	41.0
42	35.765970406002864	39.0	35.0	41.0	27.0	41.0
43	35.71122695996015	39.0	35.0	41.0	27.0	41.0
44	35.68796702783486	39.0	35.0	41.0	27.0	41.0
45	35.671897378417086	39.0	35.0	41.0	27.0	41.0
46	35.56306821719908	39.0	35.0	40.0	27.0	41.0
47	35.57239126035245	38.0	35.0	41.0	27.0	41.0
48	35.47467152375615	38.0	35.0	40.0	27.0	41.0
49	35.400478314340866	38.0	35.0	40.0	27.0	41.0
50	35.283624058160534	38.0	35.0	40.0	26.0	41.0
51	34.166728937044645	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	23.0
9	47.0
10	48.0
11	35.0
12	28.0
13	16.0
14	24.0
15	61.0
16	71.0
17	129.0
18	228.0
19	361.0
20	591.0
21	1025.0
22	1464.0
23	2357.0
24	3611.0
25	5743.0
26	7552.0
27	8118.0
28	7912.0
29	7648.0
30	8279.0
31	9974.0
32	12930.0
33	18040.0
34	30695.0
35	36366.0
36	41919.0
37	63789.0
38	111545.0
39	133233.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.14744068746497	20.53132200012454	22.703390622081077	17.61784669032941
2	38.26670402889346	21.467129024223176	23.55318668659319	16.71298026029018
3	31.12195653527617	21.639150943396228	30.904788592066758	16.334103929260852
4	26.598791954667167	24.43937200323806	30.072895261224236	18.888940780870538
5	23.5551326359051	28.81989849928389	29.00437449405318	18.620594370757832
6	24.058355127965626	31.981287751416655	28.960396039603957	14.999961081013764
7	76.25922379973846	5.393198517965004	13.624758702285323	4.722818980011208
8	77.39410143844573	4.681370259667476	13.418488075222617	4.506040226664176
9	71.29841521888038	7.117115013388132	15.362686032754219	6.221783734977271
10	36.33204122299023	28.808611993274802	21.39649106420076	13.462855719534216
11	25.294032941029954	24.460388255806713	31.373178591444052	18.872400211719285
12	23.284645681549286	21.989616414471634	34.7877358490566	19.938002054922475
13	23.072731801482036	23.091607509807584	35.18548788841148	18.650172800298897
14	19.89947225854661	25.86380689955788	34.76633040662557	19.47039043526994
15	18.438064325300456	25.33334111713058	36.82670153807834	19.40189301949063
16	21.704924030138866	26.495462046204622	33.12511675695872	18.6744971666978
17	22.471238869169934	24.852497042157047	31.506476119310044	21.169787969362975
18	23.64347873466592	24.073922722460924	32.60788342985242	19.674715113020735
19	23.02174792950993	25.665125474811635	31.132075471698112	20.181051123980325
20	23.856170994457937	25.458854847748924	30.77460458309982	19.910369574693316
21	23.442072980882994	25.460995391992032	32.805786474873905	18.291145152251072
22	23.122158914004608	23.293986238246468	32.59834827822405	20.98550656952488
23	21.103625692757955	25.02374058160533	32.486650787720286	21.385982937916435
24	20.827884675259977	23.985965813562487	33.303365713929885	21.882783797247647
25	21.915670340618966	25.99457469331839	30.90868049069058	21.181074475372068
26	21.061009402827075	25.532995516532786	31.295535213898752	22.11045986674139
27	21.138458185441188	24.833815928762686	32.60768883492123	21.4200370508749
28	20.495516532785356	25.280605890777757	32.84626222056168	21.37761535587521
29	20.97966872158914	24.435480104614236	33.41545239429603	21.169398779500593
30	22.047800298897815	23.4307864748739	33.55225263092347	20.969160595304814
31	22.622439130705523	25.33139516781867	31.070972663304065	20.975193038171742
32	22.837077339809454	25.400087178529173	31.89449841210536	19.868337069556013
33	22.613487763870726	24.28155551404197	31.724422442244226	21.380534279843076
34	21.93026496045831	25.469362974033256	31.73454137866617	20.86583068684227
35	21.951670402889345	26.045947755152877	31.157372812752975	20.84500902920481
36	24.423220623949188	26.119115449280777	29.460505012765424	19.997158914004608
37	23.664494987234573	26.457905224484712	29.402126533408058	20.475473254872657
38	22.744060962700043	26.521926956846624	29.713478423314026	21.0205336571393
39	23.35956473005791	24.812994271125223	29.324288560931567	22.503152437885298
40	24.114982252942273	24.59582632791581	30.54906905784918	20.740122361292734
41	22.095086867177283	25.211524690204868	30.0581060464537	22.635282396164143
42	23.648927392739274	25.25453016999813	29.9960302634037	21.100512173858895
43	23.03439660003736	24.414463852045582	30.571447474936175	21.979692072980882
44	22.477271312036866	25.038918986238244	29.7496730805156	22.73413662120929
45	22.698331153870104	24.13638769537331	29.78606233264836	23.379218818108228
46	22.270416900180585	25.48454137866617	28.93996357182888	23.305078149324366
47	21.769334952363163	25.771958092035618	30.390863378790712	22.06784357681051
48	22.141011270938414	24.518961330095273	30.777328912136497	22.562698486829817
49	23.081683168316832	24.28330686842269	30.816637088237126	21.81837287502335
50	20.874392863814684	24.637469643190734	32.097266330406626	22.39087116258796
51	21.72496730805156	23.723651846316706	30.370041721153246	24.181339124478484
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	215.0
1	363.5
2	512.0
3	5761.5
4	11011.0
5	7166.5
6	3322.0
7	3299.0
8	3276.0
9	3253.5
10	3231.0
11	3184.0
12	3137.0
13	3089.5
14	3042.0
15	2881.0
16	2720.0
17	2612.0
18	2504.0
19	2404.0
20	2304.0
21	2347.5
22	2391.0
23	2521.0
24	2651.0
25	3091.0
26	4501.5
27	5472.0
28	6163.5
29	6855.0
30	7467.5
31	8080.0
32	9283.0
33	10486.0
34	12347.5
35	14209.0
36	15646.5
37	17084.0
38	18192.5
39	19301.0
40	20789.5
41	22278.0
42	24508.5
43	26739.0
44	29207.0
45	31675.0
46	36608.5
47	41542.0
48	43977.5
49	46413.0
50	45619.5
51	44826.0
52	39454.0
53	34082.0
54	31184.0
55	28286.0
56	25983.0
57	23680.0
58	22424.0
59	21168.0
60	19780.5
61	18393.0
62	16880.5
63	15368.0
64	13587.0
65	11806.0
66	10135.0
67	8464.0
68	6875.5
69	5287.0
70	4518.0
71	3749.0
72	2903.0
73	2057.0
74	1714.0
75	1059.0
76	747.0
77	557.0
78	367.0
79	251.0
80	135.0
81	106.0
82	77.0
83	47.5
84	18.0
85	15.0
86	12.0
87	7.0
88	2.0
89	3.5
90	5.0
91	3.0
92	1.0
93	3.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	513888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.8004011538681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.47336006081612	28.84468803389204
2	8.637008522259032	6.875128079105741
3	3.759059948757812	4.4883628176599935
4	2.4714563774825637	3.9345982103236676
5	1.913590200734216	3.80808288166664
6	1.5691935467042248	3.7472719588133527
7	1.4118050703669596	3.933328570616898
8	1.1851850011321772	3.7736670789286615
9	1.003935237528078	3.5961322667509226
>10	5.518148961922637	31.720245264566106
>50	0.033070895205638676	0.8964148998626174
>100	0.019743818033097618	1.3981557839782235
>500	0.0024679772541372023	0.7166604327599494
>1k	0.0014807863524823213	0.8516236228109595
>5k	4.935954508274405E-4	1.4156400982643078
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7206	1.4022510741640202	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTT	1649	0.3208870415343421	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTG	1441	0.2804112958465658	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	1245	0.24227068933308427	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	981	0.19089762749859893	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	741	0.14419484401270316	Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	738	0.14361105921912948	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	650	0.126486705274301	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	538	0.104692072980883	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1083893766735164	0.0
2	0.0	0.0	0.0	0.4569088984370135	0.0
3	0.0	0.0	0.0	0.8966934429291986	0.0
4	0.0	0.0	0.0	1.3353104178342363	0.0
5	0.0	0.0	0.0	2.4199825642941653	0.0
6	0.0	0.0	0.0	3.607400834423065	0.0
7	0.0	0.0	0.0	4.436375241297715	0.0
8	0.0	0.0	0.0	5.469674325923158	0.0
9	0.0	0.0	0.0	5.996831994520207	0.0
10	0.0	0.0	0.0	6.786109035431845	0.0
11	0.0	0.0	0.0	7.728337692259792	0.0
12	0.0	0.0	0.0	8.484144405006539	0.0
13	0.0	0.0	0.0	8.892988355439318	0.0
14	0.0	0.0	0.0	9.105291425368952	0.0
15	0.0	0.0	0.0	9.338999937729621	0.0
16	0.0	0.0	0.0	9.773919608942027	0.0
17	0.0	0.0	0.0	10.340774643502087	0.0
18	0.0	0.0	0.0	10.921640513107915	0.0
19	0.0	0.0	0.0	11.271911389252132	0.0
20	0.0	0.0	0.0	11.609144405006539	0.0
21	0.0	0.0	0.0	12.0672208730307	0.0
22	1.9459493119123233E-4	0.0	0.0	12.53619465720157	0.0
23	1.9459493119123233E-4	0.0	0.0	12.98784949249642	0.0
24	1.9459493119123233E-4	0.0	0.0	13.356996076966187	0.0
25	1.9459493119123233E-4	0.0	0.0	13.69072638395915	0.0
26	1.9459493119123233E-4	0.0	0.0	14.007916121800859	0.0
27	3.8918986238246466E-4	0.0	0.0	14.34748427672956	0.0
28	5.837847935736969E-4	0.0	0.0	14.66506320443365	0.0
29	5.837847935736969E-4	0.0	0.0	15.008134068123793	0.0
30	5.837847935736969E-4	0.0	0.0	15.429626689084003	0.0
31	7.783797247649293E-4	0.0	0.0	15.801303007659257	0.0
32	7.783797247649293E-4	0.0	0.0	16.199249641945325	0.0
33	7.783797247649293E-4	0.0	0.0	16.576374618593935	0.0
34	9.729746559561616E-4	0.0	0.0	16.955445544554454	0.0
35	9.729746559561616E-4	0.0	0.0	17.353781368702908	0.0
36	9.729746559561616E-4	0.0	0.0	17.70930630798929	0.0
37	9.729746559561616E-4	0.0	0.0	18.07903667725263	0.0
38	0.0011675695871473939	0.0	0.0	18.478929260850613	0.0
39	0.0011675695871473939	0.0	0.0	18.95062737405816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCAA	30	2.1631495E-6	45.000004	18
TGTTACG	30	2.1631495E-6	45.000004	1
TTGCACG	25	3.887785E-5	45.0	1
CGTTTTT	3405	0.0	43.480175	1
CGTATGG	50	1.0786607E-9	40.5	2
ATTAGCG	40	3.4532422E-7	39.375004	1
TACGAAT	40	3.4532422E-7	39.375004	12
CGTAAGG	80	0.0	39.375004	2
CACGGGT	35	6.2415493E-6	38.571426	4
CGTTCTG	175	0.0	38.571426	1
CTAACGG	35	6.2415493E-6	38.571426	2
TTACACG	430	0.0	38.197674	34
CGGGTCC	30	1.1390435E-4	37.500004	6
TAGGGCC	90	0.0	37.5	5
TAGACGG	85	0.0	37.058823	2
CGGGTAC	55	2.743036E-9	36.81818	6
TTTAGCG	25	0.002106027	36.0	1
CGGCGAA	25	0.002106027	36.0	31
AACCCGC	25	0.002106027	36.0	21
TTATGCG	25	0.002106027	36.0	1
>>END_MODULE