##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935533.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350661
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.270258169571182	31.0	31.0	33.0	28.0	34.0
2	31.319091658325277	31.0	31.0	34.0	28.0	34.0
3	31.056191022098265	31.0	31.0	34.0	27.0	34.0
4	34.796906413886916	35.0	35.0	37.0	32.0	37.0
5	34.865921787709496	35.0	35.0	37.0	33.0	37.0
6	35.052957129535365	37.0	35.0	37.0	32.0	37.0
7	35.450027234280405	37.0	35.0	37.0	33.0	37.0
8	35.3494058364061	37.0	35.0	37.0	33.0	37.0
9	37.315039311471764	39.0	37.0	39.0	34.0	39.0
10	36.60618089836053	38.0	35.0	39.0	32.0	39.0
11	36.78393377079287	39.0	37.0	39.0	33.0	39.0
12	36.8392692657581	39.0	35.0	39.0	33.0	39.0
13	36.85764884033297	39.0	37.0	39.0	33.0	39.0
14	37.80843036436901	40.0	37.0	41.0	33.0	41.0
15	38.042254485101	40.0	37.0	41.0	33.0	41.0
16	38.039225919050025	40.0	37.0	41.0	33.0	41.0
17	37.949395570080505	40.0	37.0	41.0	33.0	41.0
18	37.861130835764456	39.0	37.0	41.0	33.0	41.0
19	37.675213382725765	39.0	37.0	41.0	33.0	41.0
20	37.667852997624486	39.0	35.0	41.0	33.0	41.0
21	37.59771973501473	39.0	35.0	41.0	33.0	41.0
22	37.701058857415	39.0	35.0	41.0	33.0	41.0
23	37.78120178748136	39.0	35.0	41.0	34.0	41.0
24	37.77887190192237	40.0	35.0	41.0	34.0	41.0
25	37.331796236250966	39.0	35.0	41.0	33.0	41.0
26	37.44329708750046	39.0	35.0	41.0	33.0	41.0
27	37.50586749025412	39.0	35.0	41.0	33.0	41.0
28	37.3679593681647	39.0	35.0	41.0	33.0	41.0
29	37.26438069816717	39.0	35.0	41.0	33.0	41.0
30	36.92915950162693	39.0	35.0	41.0	32.0	41.0
31	36.86199206641172	39.0	35.0	41.0	31.0	41.0
32	36.7800896022084	39.0	35.0	41.0	31.0	41.0
33	36.63362906054566	39.0	35.0	41.0	31.0	41.0
34	36.398852452938876	39.0	35.0	41.0	30.0	41.0
35	36.340688014920396	39.0	35.0	41.0	30.0	41.0
36	36.15876587359301	39.0	35.0	41.0	29.0	41.0
37	36.163536863238285	39.0	35.0	41.0	30.0	41.0
38	35.97234936306005	39.0	35.0	41.0	29.0	41.0
39	35.84369519279304	39.0	35.0	41.0	28.0	41.0
40	35.76474714895583	39.0	35.0	41.0	28.0	41.0
41	35.560875033151675	39.0	35.0	41.0	26.0	41.0
42	35.603243588537076	39.0	35.0	41.0	27.0	41.0
43	35.57667376754187	38.0	35.0	41.0	27.0	41.0
44	35.54046500751438	38.0	35.0	40.0	27.0	41.0
45	35.53517784983217	38.0	35.0	40.0	27.0	41.0
46	35.43072083864473	38.0	35.0	40.0	27.0	41.0
47	35.42123304273928	38.0	35.0	40.0	27.0	41.0
48	35.333937905840685	38.0	35.0	40.0	27.0	41.0
49	35.25035005318527	38.0	35.0	40.0	26.0	41.0
50	35.14957180866991	38.0	34.0	40.0	26.0	41.0
51	33.9963183815708	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	25.0
10	29.0
11	20.0
12	23.0
13	23.0
14	20.0
15	35.0
16	50.0
17	81.0
18	163.0
19	264.0
20	432.0
21	693.0
22	1109.0
23	1658.0
24	2493.0
25	3746.0
26	5120.0
27	5452.0
28	5544.0
29	5684.0
30	6406.0
31	7347.0
32	9959.0
33	14607.0
34	25136.0
35	26042.0
36	27524.0
37	41716.0
38	72702.0
39	86526.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.2960722749322	20.33587995243269	23.109498917758174	17.25854885487693
2	37.79804426497386	21.808527324110752	24.15409754720371	16.239330863711675
3	30.966089756203285	22.303877534142664	31.204496650611276	15.52553605904278
4	27.889329010069552	23.680135515497874	29.815405762260415	18.615129712172156
5	23.816164329651716	28.516145222879075	28.96900425196985	18.698686195499356
6	23.989836337659447	32.39424971696311	28.878033200156278	14.737880745221169
7	76.42994230895366	5.502465344021719	13.487100076712267	4.580492270312353
8	78.55364582887746	4.555396807743091	12.591363168416219	4.299594194963227
9	73.11876712836614	6.5156946452556745	14.349471426819635	6.016066799558549
10	39.64740875090187	26.06163787817864	20.67153176429657	13.61942160662292
11	28.684398892377537	23.698101585291777	29.5923983562472	18.02510116608348
12	26.477709240548563	20.843207542327207	33.817561690635685	18.861521526488545
13	24.95373024088792	20.876858276226898	35.13507347552194	19.034338007363235
14	19.84195562095585	24.35001326067056	35.64696387679269	20.1610672415809
15	19.35459033083234	24.07196694243158	37.070275850465265	19.503166876270814
16	23.615685804808635	23.95561525233773	33.42088227661474	19.007816666238902
17	24.30267409264218	23.416347982809608	31.305448852310352	20.97552907223786
18	24.950022956644737	23.462261272282916	32.10565189741659	19.482063873655754
19	23.843826373620107	25.91505756271727	30.324729582132033	19.916386481530594
20	23.802475895523028	26.182552379648723	30.34725846330216	19.66771326152609
21	24.164363872800227	25.719712200672447	31.923139442367415	18.192784484159915
22	22.242849931985592	23.191914698241323	33.25491001280439	21.310325356968697
23	21.570975956835806	24.340887637918105	32.72163143320757	21.366504972038523
24	22.463005580888666	24.448684056681525	31.969337907551736	21.118972454878072
25	22.912442501447266	25.040423657036282	30.16674223823009	21.880391603286363
26	20.708319431017422	24.481764439159186	31.06875301216845	23.741163117654942
27	21.387037623231553	23.812171869697515	32.622105110063565	22.178685397007367
28	21.149201080245593	25.396893295804208	31.60773510598555	21.846170517964644
29	22.415951588571296	24.84507829499146	31.827320403466597	20.911649712970647
30	24.397352428698944	23.633081523180508	31.96106781193232	20.008498236188228
31	24.300392686954066	24.80315746547235	30.73395672743761	20.16249312013597
32	25.340998856445403	25.35269106059699	29.428422322413955	19.877887760543658
33	25.02616487148557	24.082803619450125	30.12539176013301	20.765639748931303
34	22.465572162287796	25.03700154850411	31.373891022953792	21.123535266254304
35	23.102084349271802	26.25070937458115	29.906091638362973	20.74111463778407
36	24.448398880970508	27.212892223543538	28.72660489760766	19.612103997878293
37	23.57690190811068	28.146272325693477	28.024502297090354	20.25232346910549
38	23.583460949464012	26.50337505453986	28.702079786460427	21.211084209535706
39	23.630229766070364	25.163334388483467	28.234106444685892	22.972329400760277
40	24.458665206567026	23.95447454949367	29.683369408060777	21.903490835878525
41	21.88267300897448	24.968844553571685	29.71245733058424	23.4360251068696
42	21.89522074025911	25.85887794764744	29.980807674648734	22.26509363744471
43	22.03039402727991	25.514100513031103	29.804854260952883	22.650651198736103
44	22.709967746627086	24.605245522028397	29.83080525065519	22.853981480689324
45	22.40026692446551	23.989551161948437	29.414733888285266	24.195448025300788
46	22.271937854509055	25.157345698552163	29.644585511362827	22.926130935575955
47	21.505385543302506	24.43442527113081	31.12521780294929	22.9349713826174
48	21.181996287012243	25.410296554221883	30.6800585180559	22.727648640709972
49	22.37859357042842	24.635474147395918	30.81665768363177	22.169274598543893
50	20.82238971542316	24.12187269185909	31.827605579177607	23.228132013540144
51	21.120398333433144	23.69182771964946	30.946412632143293	24.2413613147741
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	152.0
1	326.5
2	501.0
3	4202.0
4	7903.0
5	5090.0
6	2277.0
7	2236.5
8	2196.0
9	2151.5
10	2107.0
11	2028.0
12	1949.0
13	1905.0
14	1861.0
15	1748.0
16	1635.0
17	1553.0
18	1471.0
19	1474.0
20	1477.0
21	1410.5
22	1344.0
23	1481.0
24	1618.0
25	1912.0
26	2624.5
27	3043.0
28	3417.5
29	3792.0
30	4553.5
31	5315.0
32	6117.5
33	6920.0
34	8166.0
35	9412.0
36	10085.0
37	10758.0
38	11614.5
39	12471.0
40	13246.5
41	14022.0
42	15164.0
43	16306.0
44	18206.5
45	20107.0
46	23402.5
47	26698.0
48	28498.0
49	30298.0
50	30236.5
51	30175.0
52	27118.5
53	24062.0
54	22330.0
55	20598.0
56	19899.0
57	19200.0
58	18428.5
59	17657.0
60	16225.5
61	14794.0
62	13181.5
63	11569.0
64	10115.5
65	8662.0
66	7223.5
67	5785.0
68	4859.5
69	3934.0
70	3288.0
71	2642.0
72	2175.0
73	1708.0
74	1350.0
75	771.5
76	551.0
77	428.0
78	305.0
79	201.5
80	98.0
81	72.5
82	47.0
83	36.0
84	25.0
85	16.5
86	8.0
87	6.5
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	350661.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.405152142392225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.09891755280539	29.725552638437208
2	9.396452967766983	7.969160353939836
3	4.694237681466841	5.971795892254557
4	3.3251527196049513	5.640144278861489
5	2.4934400684500386	5.286735273028038
6	2.0380473802823036	5.185422554056499
7	1.6831197195983978	4.996106344840142
8	1.2972090602397996	4.400667804796667
9	1.055874935216746	4.029708354408418
>10	3.8727244819434286	22.148826209682536
>50	0.024449145068265826	0.7035634491930324
>100	0.01697857296407349	1.6427040172726388
>500	0.002716571674251758	0.8121362778241307
>1k	0.0	0.0
>5k	6.791429185629395E-4	1.4874765514048351
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5165	1.4729325473890738	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTT	854	0.24354005720624763	No Hit
CGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTG	748	0.21331143183872742	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC	664	0.18935667211352275	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC	554	0.1579873439019452	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT	460	0.1311808270665971	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	456	0.13004012422253972	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	422	0.12034415004805211	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT	403	0.11492581153877961	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	376	0.10722606734139241	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08697859185937415	0.0
2	0.0	0.0	0.0	0.39411283262181995	0.0
3	0.0	0.0	0.0	0.7406013215042448	0.0
4	0.0	0.0	0.0	1.0919377974739135	0.0
5	0.0	0.0	0.0	2.027599305311968	0.0
6	0.0	0.0	0.0	3.017159022531733	0.0
7	0.0	0.0	0.0	3.743786734196275	0.0
8	0.0	0.0	0.0	4.745038655567628	0.0
9	0.0	0.0	0.0	5.387824708193953	0.0
10	0.0	0.0	0.0	6.285843022178115	0.0
11	0.0	0.0	0.0	7.202682933089223	0.0
12	0.0	0.0	0.0	7.954406107323027	0.0
13	0.0	0.0	0.0	8.355363157009192	0.0
14	0.0	0.0	0.0	8.613732351188185	0.0
15	0.0	0.0	0.0	8.825332728760825	0.0
16	0.0	0.0	0.0	9.167543581978036	0.0
17	0.0	0.0	0.0	9.558804657489713	0.0
18	0.0	0.0	0.0	9.980579534079924	0.0
19	0.0	0.0	0.0	10.276306746401795	0.0
20	0.0	0.0	0.0	10.555493767484835	0.0
21	0.0	0.0	0.0	10.890004876504658	0.0
22	0.0	0.0	0.0	11.240771001052298	0.0
23	0.0	0.0	0.0	11.572430352961977	0.0
24	0.0	0.0	0.0	11.852758076889074	0.0
25	0.0	0.0	0.0	12.100290594049524	0.0
26	0.0	0.0	0.0	12.366644708136919	0.0
27	0.0	0.0	0.0	12.661231217614734	0.0
28	0.0	0.0	0.0	12.942699644385888	0.0
29	0.0	0.0	0.0	13.234149221042545	0.0
30	0.0	0.0	0.0	13.591189211232502	0.0
31	0.0	0.0	0.0	13.891479234930603	0.0
32	0.0	0.0	0.0	14.238252899524042	0.0
33	0.0	0.0	0.0	14.597574295402111	0.0
34	0.0	0.0	0.0	14.962884381211483	0.0
35	0.0	0.0	0.0	15.32790929130984	0.0
36	0.0	0.0	0.0	15.6353287077833	0.0
37	0.0	0.0	0.0	15.97554333102341	0.0
38	0.0	0.0	0.0	16.399599613301735	0.0
39	0.0017110542660860489	0.0	0.0	17.034115570308646	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	25	3.885243E-5	45.0	1
CGCATAC	20	7.0265745E-4	45.0	26
GGCCGAT	20	7.0265745E-4	45.0	8
GTTAGCG	20	7.0265745E-4	45.0	1
ATATGCG	20	7.0265745E-4	45.0	1
AGACACG	20	7.0265745E-4	45.0	24
CTCGAGG	20	7.0265745E-4	45.0	2
CGTAAGG	50	2.1827873E-11	45.0	2
CGGTCTA	25	3.885243E-5	45.0	31
CGCACGG	25	3.885243E-5	45.0	2
CGTTTTT	2190	0.0	43.356163	1
ATAGCGG	45	1.9217623E-8	40.0	2
ACGGGAT	165	0.0	39.545456	5
ACGGGTC	40	3.4490586E-7	39.375	5
AAACGGG	80	0.0	39.375	3
CACGACG	35	6.2358795E-6	38.571426	26
GCTACGG	35	6.2358795E-6	38.571426	2
GCGTAAG	35	6.2358795E-6	38.571426	1
ATCGTAG	30	1.1383045E-4	37.500004	1
GCGTATG	30	1.1383045E-4	37.500004	1
>>END_MODULE