##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935532.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	365611
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22789795711836	31.0	31.0	33.0	28.0	34.0
2	31.241543607823616	31.0	31.0	34.0	28.0	34.0
3	31.068974948784362	31.0	31.0	34.0	27.0	34.0
4	34.89013733175424	35.0	35.0	37.0	32.0	37.0
5	34.88749791445006	35.0	35.0	37.0	33.0	37.0
6	35.077861442899696	37.0	35.0	37.0	32.0	37.0
7	35.57935893613704	37.0	35.0	37.0	33.0	37.0
8	35.28636446934036	37.0	35.0	37.0	33.0	37.0
9	37.15104578363342	39.0	37.0	39.0	33.0	39.0
10	36.53466115625624	38.0	35.0	39.0	32.0	39.0
11	36.714119651761024	39.0	37.0	39.0	32.0	39.0
12	36.7448763850103	39.0	35.0	39.0	33.0	39.0
13	36.745798129706166	39.0	35.0	39.0	33.0	39.0
14	37.78771152946711	40.0	36.0	41.0	33.0	41.0
15	37.99174532494919	40.0	37.0	41.0	33.0	41.0
16	37.91157541758864	39.0	36.0	41.0	33.0	41.0
17	37.83831449272588	39.0	36.0	41.0	33.0	41.0
18	37.81649622139378	39.0	36.0	41.0	33.0	41.0
19	37.7040761902678	39.0	36.0	41.0	33.0	41.0
20	37.84656916777668	39.0	36.0	41.0	33.0	41.0
21	37.76182609385385	39.0	36.0	41.0	33.0	41.0
22	37.887749548016885	40.0	36.0	41.0	33.0	41.0
23	37.93491169576408	40.0	36.0	41.0	34.0	41.0
24	37.941413141289516	40.0	36.0	41.0	34.0	41.0
25	37.52086780758785	39.0	35.0	41.0	33.0	41.0
26	37.624401344598496	39.0	35.0	41.0	33.0	41.0
27	37.687383585286	40.0	35.0	41.0	33.0	41.0
28	37.62864902861238	40.0	35.0	41.0	33.0	41.0
29	37.6302873819442	40.0	35.0	41.0	33.0	41.0
30	37.34204933658998	39.0	35.0	41.0	33.0	41.0
31	37.30716800096277	39.0	35.0	41.0	33.0	41.0
32	37.320791770488306	39.0	35.0	41.0	33.0	41.0
33	37.341340933396424	39.0	35.0	41.0	33.0	41.0
34	37.240337407791344	40.0	35.0	41.0	32.0	41.0
35	37.276208866801056	40.0	35.0	41.0	33.0	41.0
36	37.19738465199351	40.0	35.0	41.0	32.0	41.0
37	37.23379219990646	40.0	35.0	41.0	33.0	41.0
38	37.09934602624101	39.0	35.0	41.0	32.0	41.0
39	37.01994196017078	39.0	35.0	41.0	32.0	41.0
40	36.90986868557018	39.0	35.0	41.0	32.0	41.0
41	36.756921974448254	39.0	35.0	41.0	31.0	41.0
42	36.81216921810339	39.0	35.0	41.0	32.0	41.0
43	36.788419932660666	39.0	35.0	41.0	32.0	41.0
44	36.76436157555435	39.0	35.0	41.0	32.0	41.0
45	36.73741490272448	39.0	35.0	41.0	32.0	41.0
46	36.60721641307291	39.0	35.0	41.0	31.0	41.0
47	36.60386585742771	39.0	35.0	41.0	31.0	41.0
48	36.54406732839001	38.0	35.0	40.0	31.0	41.0
49	36.522027510113205	39.0	35.0	40.0	31.0	41.0
50	36.416839208885946	38.0	35.0	40.0	31.0	41.0
51	35.316401858806216	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	31.0
10	25.0
11	11.0
12	14.0
13	20.0
14	22.0
15	33.0
16	37.0
17	72.0
18	102.0
19	182.0
20	285.0
21	504.0
22	717.0
23	1032.0
24	1372.0
25	1938.0
26	2604.0
27	3220.0
28	3618.0
29	4177.0
30	5448.0
31	7250.0
32	9763.0
33	14568.0
34	28743.0
35	29994.0
36	28871.0
37	45043.0
38	78598.0
39	97292.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.59879762917418	21.037386730705588	23.924881910008178	11.43893373011206
2	32.84556536865686	23.439940264379356	25.52877238376307	18.185721983200725
3	33.86632240277235	23.066866150088483	25.96147271280131	17.10533873433786
4	30.21818271332099	25.78013243584028	24.69701403951194	19.304670811326794
5	25.307225439059543	30.346461129451797	23.22632524732571	21.11998818416295
6	25.262095505879202	34.98800637836389	24.16420731323729	15.585690802519617
7	79.11085826192318	6.802585261384368	8.818662458186433	5.2678940185060075
8	79.92018839695743	6.072300888102382	8.403740587673784	5.6037701272664115
9	73.09517492635615	8.454340815785082	11.176086058679854	7.274398199178908
10	42.19211128768007	25.985815525244043	17.27382381821116	14.548249368864722
11	33.87835705162044	25.121782440900304	22.611190582340246	18.388669925139016
12	32.99107521382015	20.53466662655117	27.285831115584596	19.18842704404408
13	27.638118109137856	22.420824318743147	28.030338255687052	21.910719316431944
14	20.978307545451315	26.94913446258455	28.149317170435246	23.923240821528893
15	21.37326283946599	25.38490362707905	32.98013462395825	20.261698909496705
16	24.408455981904265	24.343359472225945	31.085224459876752	20.16296008599304
17	23.3056445238245	24.997880260714254	27.878537571353156	23.81793764410808
18	24.997333231221162	25.173203213251245	27.87060564370328	21.958857911824317
19	25.311328160257762	28.606633826662765	25.23638511970373	20.845652893375746
20	29.31804568243297	25.044377767627342	25.91552223538132	19.722054314558367
21	26.619275678248194	27.271334834017573	25.70600993952589	20.403379548208342
22	23.51652439341267	25.594415922934484	27.12035469392332	23.76870498972952
23	24.849908782832024	26.015081603124635	27.68078640959927	21.454223204444066
24	26.210918161652685	23.111449053775736	27.820278930338528	22.85735385423305
25	23.860332429822954	24.640122972230046	26.3271619289354	25.1723826690116
26	22.13336032012166	27.113790340006183	26.784752099909465	23.968097239962695
27	22.749315529346763	27.12391038562844	26.88622607087861	23.240548014146185
28	20.13670267032447	29.015538372751365	28.204020119744754	22.643738837179406
29	23.735062675904175	27.23358979899401	27.067019318346546	21.96432820675527
30	25.233649972238254	25.875042052892283	26.911389427560987	21.97991854730848
31	24.849088238592383	27.36843256904196	24.975725566243902	22.806753626121754
32	25.977336568101066	27.49944613263824	25.35864621141049	21.164571087850202
33	26.48197127548132	27.101208661664995	25.16472425610827	21.252095806745423
34	22.52448640768467	27.1564586404676	27.676410173654514	22.642644778193215
35	22.1289840841769	26.60915563262593	27.378005585171124	23.883854698026045
36	22.860909545938167	30.371350971387624	25.360834329382868	21.40690515329134
37	23.789765625213683	29.3705605137701	25.8118601464398	21.02781371457642
38	22.69187743257178	28.70810779763191	24.992409965783306	23.607604804013008
39	24.85483204826988	24.858387739975	26.497014586541432	23.789765625213683
40	24.623712087437195	23.308379671289977	27.721813621581408	24.34609461969142
41	19.916523299353685	26.74618652064626	27.00274335290787	26.334546827092183
42	21.680966929331998	26.60177073446915	26.678628378249016	25.03863395794984
43	23.011616171285876	25.263463079611938	27.521327312362047	24.203593436740142
44	22.41343942058636	25.76563615427326	26.923150561662535	24.89777386347785
45	23.5805268441048	24.34062432476047	26.217482515569827	25.861366315564904
46	22.66452595791702	26.97840054046514	25.784235157038495	24.57283834457935
47	21.164024058357107	25.814595293905274	28.8661993211364	24.155181326601223
48	21.458052410895732	23.96618263673686	29.320780829898446	25.254984122468965
49	21.85628988186898	23.92023215931687	29.500206503633642	24.723271455180505
50	20.304640724704672	24.14916400217718	29.540139656629588	26.00605561648856
51	19.572988777689947	23.690206257470372	27.710052487479864	29.026752477359818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	186.0
1	436.5
2	687.0
3	1206.0
4	1725.0
5	1221.0
6	717.0
7	724.0
8	731.0
9	698.5
10	666.0
11	626.0
12	586.0
13	640.5
14	695.0
15	657.5
16	620.0
17	624.0
18	628.0
19	748.0
20	868.0
21	910.0
22	952.0
23	1181.5
24	1411.0
25	1517.0
26	2201.5
27	2780.0
28	3468.0
29	4156.0
30	4749.0
31	5342.0
32	5898.5
33	6455.0
34	7929.5
35	9404.0
36	9488.0
37	9572.0
38	10650.0
39	11728.0
40	13203.5
41	14679.0
42	16058.0
43	17437.0
44	19683.0
45	21929.0
46	24923.0
47	27917.0
48	30304.5
49	32692.0
50	32856.0
51	33020.0
52	29870.0
53	26720.0
54	24732.0
55	22744.0
56	21688.0
57	20632.0
58	20549.0
59	20466.0
60	20773.0
61	21080.0
62	19106.0
63	17132.0
64	14215.5
65	11299.0
66	9016.5
67	6734.0
68	5234.5
69	3735.0
70	3143.0
71	2551.0
72	2042.0
73	1533.0
74	1172.0
75	642.0
76	473.0
77	355.0
78	237.0
79	196.5
80	156.0
81	114.0
82	72.0
83	40.5
84	9.0
85	8.5
86	8.0
87	7.5
88	7.0
89	5.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	365611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.76818126171098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.13353039620284	25.07951659862319
2	9.084396031995832	6.316958557871981
3	3.319188444331435	3.462064364228755
4	1.9678610512308767	2.7367579890822493
5	1.4773942644677194	2.5683155791012915
6	1.280005838620268	2.6702085007918708
7	1.1518971914837601	2.8034559243854216
8	0.9768837937514386	2.717157825022232
9	0.8421436171978195	2.635182173801405
>10	7.6532594499977975	44.3125214802244
>50	0.07877772272237372	1.841127663722111
>100	0.030723311861725753	1.7477291910459374
>500	0.003151108908894949	0.8205918596416907
>1k	7.877772272237373E-4	0.2884122924575101
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	1053	0.2880110281145808	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	901	0.24643678663935167	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	762	0.20841823686924082	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	744	0.2034949714313847	No Hit
CTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGCT	589	0.1611001857165129	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.470294930951203E-4	0.0	0.0	0.16848508387329703	0.0
2	5.470294930951203E-4	0.0	0.0	0.8454340815785083	0.0
3	5.470294930951203E-4	0.0	0.0	1.1039055170659526	0.0
4	5.470294930951203E-4	0.0	0.0	1.4840910147670612	0.0
5	5.470294930951203E-4	0.0	0.0	2.5756883682383735	0.0
6	5.470294930951203E-4	0.0	0.0	3.2036782263115717	0.0
7	5.470294930951203E-4	0.0	0.0	3.8149836848453686	0.0
8	5.470294930951203E-4	0.0	0.0	4.798542713430394	0.0
9	5.470294930951203E-4	0.0	0.0	5.189121771500311	0.0
10	5.470294930951203E-4	0.0	0.0	6.085429595936665	0.0
11	5.470294930951203E-4	0.0	0.0	7.298193982128547	0.0
12	5.470294930951203E-4	0.0	0.0	8.356696051267605	0.0
13	5.470294930951203E-4	0.0	0.0	8.772164951273348	0.0
14	5.470294930951203E-4	0.0	0.0	8.953231713487833	0.0
15	5.470294930951203E-4	0.0	0.0	9.31563875266335	0.0
16	5.470294930951203E-4	0.0	0.0	9.949919449907142	0.0
17	5.470294930951203E-4	0.0	0.0	10.69223847203722	0.0
18	5.470294930951203E-4	0.0	0.0	11.473123073430504	0.0
19	5.470294930951203E-4	0.0	0.0	11.946850614450877	0.0
20	5.470294930951203E-4	0.0	0.0	12.45558804302934	0.0
21	5.470294930951203E-4	0.0	0.0	13.062243750871827	0.0
22	5.470294930951203E-4	0.0	0.0	13.694883359636334	0.0
23	5.470294930951203E-4	0.0	0.0	14.28731630065835	0.0
24	5.470294930951203E-4	0.0	0.0	14.763505474397652	0.0
25	5.470294930951203E-4	0.0	0.0	15.215625350440769	0.0
26	5.470294930951203E-4	0.0	0.0	15.614409850907112	0.0
27	5.470294930951203E-4	0.0	0.0	16.013467866120003	0.0
28	5.470294930951203E-4	0.0	0.0	16.439330326494552	0.0
29	5.470294930951203E-4	0.0	0.0	16.853705167514107	0.0
30	5.470294930951203E-4	0.0	0.0	17.35642527166852	0.0
31	8.205442396426804E-4	0.0	0.0	17.809092177204732	0.0
32	0.0010940589861902405	0.0	0.0	18.27953754126654	0.0
33	0.0010940589861902405	0.0	0.0	18.742598007171555	0.0
34	0.0013675737327378005	0.0	0.0	19.170922100265035	0.0
35	0.0013675737327378005	0.0	0.0	19.661881070317907	0.0
36	0.0013675737327378005	0.0	0.0	20.143267024241613	0.0
37	0.0016410884792853607	0.0	0.0	20.60796857862592	0.0
38	0.0016410884792853607	0.0	0.0	21.116979521950928	0.0
39	0.0016410884792853607	0.0	0.0	21.634742937165456	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCTA	20	7.026968E-4	45.000004	9
TTCTAGA	40	6.7939254E-9	45.000004	39
GGTAATT	20	7.026968E-4	45.000004	8
GAATCGG	20	7.026968E-4	45.000004	2
GCCGATT	20	7.026968E-4	45.000004	9
GTCCGTA	20	7.026968E-4	45.000004	26
CGGGACG	20	7.026968E-4	45.000004	6
ACGCTCC	20	7.026968E-4	45.000004	22
CGTAAGG	30	2.161427E-6	45.000004	2
AAGGATC	20	7.026968E-4	45.000004	17
CTATCGC	25	3.8855702E-5	45.0	35
AATATGC	25	3.8855702E-5	45.0	16
GTAGACG	25	3.8855702E-5	45.0	1
CACGTGA	45	3.8380676E-10	45.0	43
GGCGATA	80	0.0	42.187504	8
CGTTAGG	70	0.0	41.785717	2
ACAACGA	65	0.0	41.53846	13
CGAGGGT	60	3.6379788E-12	41.250004	4
ATAGGGA	155	0.0	40.64516	4
GCGTTAG	50	1.0768417E-9	40.5	1
>>END_MODULE