SRR2935531.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935531.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427573
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	3074	0.7189415608562748	TruSeq Adapter, Index 16 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC	2567	0.6003653177352172	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC	2442	0.5711305437901831	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG	2228	0.5210806107962851	No Hit
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	2101	0.49137808046813053	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	1366	0.319477609671331	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1148	0.2684921639111918	No Hit
TCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	912	0.2132969107029677	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCC	623	0.1457061133420492	No Hit
ACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	608	0.14219794046864512	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT	565	0.13214117823155344	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTG	510	0.1192778776957385	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT	495	0.11576970482233442	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTT	481	0.11249541014049062	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT	454	0.1061806989683633	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC	451	0.10547906439368247	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	30	2.162291E-6	45.000004	25
ACGTTAG	20	7.02831E-4	45.000004	1
CGTTGCC	20	7.02831E-4	45.000004	28
GAGTACG	30	2.162291E-6	45.000004	1
TAGCGCG	20	7.02831E-4	45.000004	1
TGAACGG	40	6.7975634E-9	45.000004	2
ATATGCG	30	2.162291E-6	45.000004	1
TACGGGT	20	7.02831E-4	45.000004	4
AACGGGC	30	2.162291E-6	45.000004	4
TCTACCG	35	1.209537E-7	45.000004	36
AGTTGCG	70	0.0	45.000004	1
CTACGAA	40	6.7975634E-9	45.000004	11
CTACCGA	20	7.02831E-4	45.000004	37
CATCGAA	20	7.02831E-4	45.000004	42
CGGTCTA	30	2.162291E-6	45.000004	31
AATAGCG	30	2.162291E-6	45.000004	1
TAGTGCG	20	7.02831E-4	45.000004	1
TCGAACG	20	7.02831E-4	45.000004	1
GCGTAAG	20	7.02831E-4	45.000004	1
ACGGGTA	25	3.886682E-5	45.0	5