##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935531.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427573
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14520093644828	31.0	31.0	33.0	28.0	34.0
2	31.102494778669374	31.0	31.0	34.0	28.0	34.0
3	30.92663942765329	31.0	30.0	34.0	27.0	34.0
4	34.77550500148513	35.0	35.0	37.0	32.0	37.0
5	34.79652363456065	35.0	35.0	37.0	32.0	37.0
6	35.0062655967519	36.0	35.0	37.0	32.0	37.0
7	35.49063434781897	37.0	35.0	37.0	33.0	37.0
8	35.335856099426294	37.0	35.0	37.0	33.0	37.0
9	37.16562785769915	39.0	37.0	39.0	34.0	39.0
10	36.42608630573025	38.0	35.0	39.0	32.0	39.0
11	36.65606340905529	39.0	35.0	39.0	32.0	39.0
12	36.86247962336256	39.0	37.0	39.0	33.0	39.0
13	36.946219709850716	39.0	37.0	39.0	33.0	39.0
14	37.93448370219822	40.0	37.0	41.0	33.0	41.0
15	38.119146905908465	40.0	37.0	41.0	33.0	41.0
16	38.088862018883326	40.0	37.0	41.0	34.0	41.0
17	38.01426423090326	39.0	37.0	41.0	33.0	41.0
18	38.03292303302594	39.0	37.0	41.0	34.0	41.0
19	37.939900321114756	39.0	37.0	41.0	34.0	41.0
20	38.036983158431426	40.0	37.0	41.0	34.0	41.0
21	38.02950841142916	40.0	37.0	41.0	34.0	41.0
22	38.1577952770638	40.0	37.0	41.0	34.0	41.0
23	38.191913427648615	40.0	37.0	41.0	34.0	41.0
24	38.19181052124433	40.0	37.0	41.0	34.0	41.0
25	37.80508357637176	40.0	37.0	41.0	33.0	41.0
26	37.93064809985663	40.0	37.0	41.0	34.0	41.0
27	37.94968812343156	40.0	37.0	41.0	34.0	41.0
28	37.88518685698115	40.0	37.0	41.0	33.0	41.0
29	37.87574519438786	40.0	37.0	41.0	33.0	41.0
30	37.60845516438129	40.0	36.0	41.0	33.0	41.0
31	37.63563882658634	40.0	36.0	41.0	33.0	41.0
32	37.6495732892395	40.0	37.0	41.0	33.0	41.0
33	37.6632434695362	40.0	37.0	41.0	33.0	41.0
34	37.65209449614452	40.0	37.0	41.0	33.0	41.0
35	37.68431121703195	40.0	37.0	41.0	33.0	41.0
36	37.55090008022022	40.0	37.0	41.0	33.0	41.0
37	37.525393324648654	40.0	37.0	41.0	33.0	41.0
38	37.42440706031485	40.0	36.0	41.0	33.0	41.0
39	37.41099648481078	40.0	36.0	41.0	33.0	41.0
40	37.36571298936088	40.0	36.0	41.0	33.0	41.0
41	37.21245962677718	39.0	36.0	41.0	32.0	41.0
42	37.242512974392675	40.0	36.0	41.0	32.0	41.0
43	37.198822189427304	39.0	36.0	41.0	32.0	41.0
44	37.1952391755326	39.0	36.0	41.0	32.0	41.0
45	37.19007280628103	39.0	36.0	41.0	32.0	41.0
46	37.09226494657053	39.0	35.0	41.0	32.0	41.0
47	37.03606869470242	39.0	35.0	41.0	32.0	41.0
48	36.99666957455218	39.0	35.0	41.0	32.0	41.0
49	36.996901113961826	39.0	35.0	41.0	32.0	41.0
50	36.89409059973384	39.0	35.0	41.0	31.0	41.0
51	35.68968339909208	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	26.0
10	22.0
11	14.0
12	8.0
13	7.0
14	12.0
15	20.0
16	31.0
17	42.0
18	81.0
19	175.0
20	267.0
21	554.0
22	821.0
23	1157.0
24	1498.0
25	2054.0
26	2711.0
27	3160.0
28	3819.0
29	4646.0
30	5941.0
31	7744.0
32	10772.0
33	15267.0
34	25653.0
35	29751.0
36	34784.0
37	54554.0
38	102139.0
39	119820.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.556646467386855	21.120370088850322	25.817813566338383	11.50516987742444
2	29.837711922876327	26.051224001515532	26.255399662747646	17.855664412860495
3	30.556185727349483	24.979360249594805	28.56635007355469	15.898103949501023
4	27.521850070046515	24.977255345870763	28.440991362878385	19.059903221204333
5	26.204648095179067	29.103568279568638	25.276385552876352	19.415398072375943
6	24.1116721589062	34.43014409235382	25.34725064491911	16.11093310382087
7	78.73392379780762	5.006864324922294	11.192240857116795	5.066971020153284
8	78.99469798139734	5.4968391362410625	9.016472041031589	6.4919908413300185
9	73.33578125840499	7.040435200538855	11.23808098266261	8.385702558393538
10	35.4157067915888	35.198200073437754	17.50835529839349	11.877737836579952
11	22.645255897823297	24.643043410131135	31.991963945337986	20.71973674670758
12	22.008639460396235	21.290633412306203	35.18253023460321	21.51819689269435
13	22.54468827545238	24.090623121665775	35.6011721974961	17.763516405385747
14	17.96909533576723	29.629326454196125	30.861630645527192	21.53994756450945
15	15.734623093600392	27.51483372429971	36.945737920776104	19.804805261323796
16	18.805911505169878	27.76929319671729	31.230456553617746	22.19433874449509
17	18.477078767836137	26.919145970395697	30.17706917883028	24.426706082937883
18	19.602968382007283	25.427938621007407	33.39570085108274	21.57339214590257
19	19.121179307393124	27.86214283876671	30.20957824745716	22.807099606383
20	20.278642477424906	28.376207103816192	31.56092643829241	19.784223980466493
21	20.51135127802738	28.39398184637477	31.896775521372962	19.19789135422489
22	18.81035519080952	24.899373908081195	31.237706777556113	25.05256412355317
23	17.792751179330782	27.238857458258593	31.782409085699985	23.185982276710643
24	20.187897739099522	25.287611706071246	31.00148980408024	23.523000750748995
25	17.961845111828858	29.369487783372666	28.58482645068795	24.083840654110524
26	19.010087166401995	26.85061966026854	30.06784806337163	24.071445109957832
27	20.29688497636661	26.816473444300737	31.069782236015836	21.816859343316814
28	18.269862690113737	26.106419254723757	32.089491151218624	23.53422690394389
29	20.63343569402184	24.778926639427652	31.11515460517853	23.472483061371978
30	21.39213654744336	24.926503778302184	31.022538841320664	22.65882083293379
31	22.833761720220874	26.700937617669968	26.388476353745443	24.07682430836372
32	21.586021568246828	29.459063130740248	27.349247964675037	21.605667336337888
33	19.752884302797415	26.25118985529956	27.97931581273841	26.01661002916461
34	21.201058064938618	24.55346806276355	28.593012187392564	25.65246168490527
35	18.497893926885002	25.321757922039044	30.92337448809911	25.25697366297685
36	22.385183348808273	25.974745832875325	29.15104555245537	22.489025265861034
37	19.043063991411994	26.966857121473993	31.764166586758286	22.22591230035573
38	18.960504989791215	27.897692323883877	28.579681130473627	24.56212155585128
39	20.284723310405475	26.487874585158554	28.080585069684005	25.14681703475196
40	20.226253762515405	22.585149202592305	31.340847060034193	25.847749974858093
41	18.818307049322573	25.43729374866982	28.887230952375383	26.857168249632224
42	21.16854899631174	24.38016432281739	31.866605234661684	22.584681446209185
43	22.369513509973736	25.491319610920236	29.42538467115557	22.713782207950455
44	20.792706742474383	24.683972093654184	28.966983415697435	25.556337748173995
45	20.980744808488843	22.46002437010756	28.22746057398386	28.33177024741974
46	23.20866846129199	23.472015304988854	29.076204531156087	24.243111702563073
47	18.032944082063178	23.86072085936203	33.689685737874	24.41664932070079
48	18.844969163160442	24.240539042455907	30.782345938588264	26.132145855795386
49	20.847668117491047	21.896144050255746	33.72804176128989	23.52814607096332
50	19.980681661377123	21.334368634127973	32.13229086027415	26.552658844220755
51	19.201165648906738	20.964841091462745	28.614061224632987	31.219932034997534
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	140.0
1	277.5
2	415.0
3	1118.0
4	1821.0
5	1217.0
6	613.0
7	635.5
8	658.0
9	649.0
10	640.0
11	647.5
12	655.0
13	711.5
14	768.0
15	764.0
16	760.0
17	705.5
18	651.0
19	757.5
20	864.0
21	927.5
22	991.0
23	1323.0
24	1655.0
25	2366.0
26	3618.0
27	4159.0
28	4964.5
29	5770.0
30	6569.0
31	7368.0
32	8880.5
33	10393.0
34	11115.5
35	11838.0
36	13449.5
37	15061.0
38	16283.0
39	17505.0
40	19386.5
41	21268.0
42	24310.5
43	27353.0
44	30142.0
45	32931.0
46	43264.5
47	53598.0
48	54555.5
49	55513.0
50	53208.0
51	50903.0
52	41153.0
53	31403.0
54	25971.0
55	20539.0
56	17321.5
57	14104.0
58	12477.0
59	10850.0
60	9718.0
61	8586.0
62	7171.5
63	5757.0
64	4752.0
65	3747.0
66	3053.5
67	2360.0
68	1835.0
69	1310.0
70	970.5
71	631.0
72	575.5
73	520.0
74	379.0
75	159.0
76	80.0
77	55.5
78	31.0
79	22.5
80	14.0
81	17.0
82	20.0
83	12.0
84	4.0
85	3.0
86	2.0
87	2.5
88	3.0
89	1.5
90	0.0
91	1.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	427573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.01709769644255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.12092685336799	27.03811224446035
2	11.924321659343434	9.066562029741306
3	4.40105843381119	5.019464053378574
4	2.218702205940263	3.3739447409017425
5	1.4417197930756405	2.740500111212578
6	1.0167052741037226	2.3191310240453773
7	0.8512528466033618	2.2653513843586377
8	0.7862441048620094	2.3912575158232823
9	0.6290045441173012	2.152163448469237
>10	5.476628285996378	34.2223160481056
>50	0.08502665968690681	2.2391321313030836
>100	0.04096174846230547	2.891052266054445
>500	0.0031031627622958692	0.7592757848823312
>1k	0.004344427867214217	3.521737217263417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	3074	0.7189415608562748	TruSeq Adapter, Index 16 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC	2567	0.6003653177352172	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC	2442	0.5711305437901831	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG	2228	0.5210806107962851	No Hit
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	2101	0.49137808046813053	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	1366	0.319477609671331	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1148	0.2684921639111918	No Hit
TCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	912	0.2132969107029677	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCC	623	0.1457061133420492	No Hit
ACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	608	0.14219794046864512	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT	565	0.13214117823155344	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTG	510	0.1192778776957385	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT	495	0.11576970482233442	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTT	481	0.11249541014049062	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT	454	0.1061806989683633	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC	451	0.10547906439368247	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3387819156027158E-4	0.0	0.0	0.3328086665902665	0.0
2	2.3387819156027158E-4	0.0	0.0	1.9664478346387635	0.0
3	2.3387819156027158E-4	0.0	0.0	2.56634539599086	0.0
4	2.3387819156027158E-4	0.0	0.0	3.478236464884359	0.0
5	2.3387819156027158E-4	0.0	0.0	6.179061821022375	0.0
6	2.3387819156027158E-4	0.0	0.0	7.3362911128625985	0.0
7	2.3387819156027158E-4	0.0	0.0	8.643670203684517	0.0
8	2.3387819156027158E-4	0.0	0.0	10.725887743145615	0.0
9	2.3387819156027158E-4	0.0	0.0	11.355955591208987	0.0
10	2.3387819156027158E-4	0.0	0.0	13.360759449263634	0.0
11	2.3387819156027158E-4	0.0	0.0	15.698371973908548	0.0
12	2.3387819156027158E-4	0.0	0.0	18.20694945658402	0.0
13	2.3387819156027158E-4	0.0	0.0	18.993014058418094	0.0
14	2.3387819156027158E-4	0.0	0.0	19.304539809576376	0.0
15	2.3387819156027158E-4	0.0	0.0	19.87520259698344	0.0
16	2.3387819156027158E-4	0.0	0.0	20.91058135102076	0.0
17	2.3387819156027158E-4	0.0	0.0	22.454879049893236	0.0
18	2.3387819156027158E-4	0.0	0.0	24.006894729087197	0.0
19	2.3387819156027158E-4	0.0	0.0	25.048588194296645	0.0
20	2.3387819156027158E-4	0.0	0.0	25.962584166914187	0.0
21	2.3387819156027158E-4	0.0	0.0	26.95422769912974	0.0
22	2.3387819156027158E-4	0.0	0.0	28.124554169697337	0.0
23	2.3387819156027158E-4	0.0	0.0	29.232435163118346	0.0
24	2.3387819156027158E-4	0.0	0.0	30.05708966655986	0.0
25	2.3387819156027158E-4	0.0	0.0	30.74469154974706	0.0
26	2.3387819156027158E-4	0.0	0.0	31.421535036122485	0.0
27	2.3387819156027158E-4	0.0	0.0	32.09791076611479	0.0
28	2.3387819156027158E-4	0.0	0.0	32.79393226419816	0.0
29	2.3387819156027158E-4	0.0	0.0	33.480130878236	0.0
30	2.3387819156027158E-4	0.0	0.0	34.27508285134936	0.0
31	2.3387819156027158E-4	0.0	0.0	34.98560479730946	0.0
32	4.6775638312054316E-4	0.0	0.0	35.65683520708745	0.0
33	4.6775638312054316E-4	0.0	0.0	36.30888760515748	0.0
34	7.016345746808148E-4	0.0	0.0	36.99906214845184	0.0
35	7.016345746808148E-4	0.0	0.0	37.7322702789933	0.0
36	7.016345746808148E-4	0.0	0.0	38.37262876748532	0.0
37	7.016345746808148E-4	0.0	0.0	39.04409305545486	0.0
38	7.016345746808148E-4	0.0	0.0	39.66527353223894	0.0
39	7.016345746808148E-4	0.0	0.0	40.324576154247346	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	30	2.162291E-6	45.000004	25
ACGTTAG	20	7.02831E-4	45.000004	1
CGTTGCC	20	7.02831E-4	45.000004	28
GAGTACG	30	2.162291E-6	45.000004	1
TAGCGCG	20	7.02831E-4	45.000004	1
TGAACGG	40	6.7975634E-9	45.000004	2
ATATGCG	30	2.162291E-6	45.000004	1
TACGGGT	20	7.02831E-4	45.000004	4
AACGGGC	30	2.162291E-6	45.000004	4
TCTACCG	35	1.209537E-7	45.000004	36
AGTTGCG	70	0.0	45.000004	1
CTACGAA	40	6.7975634E-9	45.000004	11
CTACCGA	20	7.02831E-4	45.000004	37
CATCGAA	20	7.02831E-4	45.000004	42
CGGTCTA	30	2.162291E-6	45.000004	31
AATAGCG	30	2.162291E-6	45.000004	1
TAGTGCG	20	7.02831E-4	45.000004	1
TCGAACG	20	7.02831E-4	45.000004	1
GCGTAAG	20	7.02831E-4	45.000004	1
ACGGGTA	25	3.886682E-5	45.0	5
>>END_MODULE