SRR2935529.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935529.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452063
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTC	2670	0.5906256428860579	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCG	2134	0.47205809809694665	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGC	2095	0.4634309819649031	No Hit
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1878	0.4154288229737891	RNA PCR Primer, Index 23 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1530	0.33844840210324667	No Hit
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1256	0.27783738107299205	RNA PCR Primer, Index 30 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	778	0.1720999064289712	RNA PCR Primer, Index 30 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCT	650	0.14378526886739237	RNA PCR Primer, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTG	553	0.12232808259025843	RNA PCR Primer, Index 30 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	520	0.11502821509391391	RNA PCR Primer, Index 23 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	512	0.11325855024631522	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCT	466	0.10308297737262286	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	25	3.8870377E-5	45.0	1
CGATTAG	20	7.0287404E-4	45.0	10
CGCGAGG	25	3.8870377E-5	45.0	2
GCGTTAG	40	6.7993824E-9	45.0	1
CCGTCGC	25	3.8870377E-5	45.0	36
ATCCGTC	20	7.0287404E-4	45.0	28
TCGTGCG	20	7.0287404E-4	45.0	1
GCTTCGG	20	7.0287404E-4	45.0	2
TAACACG	20	7.0287404E-4	45.0	1
CGTAAGG	55	1.8189894E-12	45.0	2
TGGGCGC	20	7.0287404E-4	45.0	6
GCTACGC	20	7.0287404E-4	45.0	1
CGACTCA	20	7.0287404E-4	45.0	32
CCTATCG	25	3.8870377E-5	45.0	40
AAGTGCG	25	3.8870377E-5	45.0	1
GCCGTCG	25	3.8870377E-5	45.0	35
TATCCGG	20	7.0287404E-4	45.0	2
TCGCAAG	30	2.1625692E-6	44.999996	1
ATTAGCG	30	2.1625692E-6	44.999996	1
CGTTTTT	905	0.0	41.270714	1