##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935529.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452063
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.232272935409444	31.0	31.0	33.0	28.0	34.0
2	31.21456080236604	31.0	31.0	34.0	28.0	34.0
3	30.986090434297875	31.0	31.0	34.0	27.0	34.0
4	34.82145630144471	35.0	35.0	37.0	32.0	37.0
5	34.86522453728794	35.0	35.0	37.0	33.0	37.0
6	35.05388850669044	36.0	35.0	37.0	32.0	37.0
7	35.559758263781816	37.0	35.0	37.0	33.0	37.0
8	35.361485014256864	37.0	35.0	37.0	33.0	37.0
9	37.28588714404851	39.0	37.0	39.0	34.0	39.0
10	36.532023191457824	38.0	35.0	39.0	32.0	39.0
11	36.72079334075118	39.0	37.0	39.0	32.0	39.0
12	36.94340390609273	39.0	37.0	39.0	33.0	39.0
13	37.05964655368831	39.0	37.0	39.0	33.0	39.0
14	38.12757292678233	40.0	37.0	41.0	33.0	41.0
15	38.30205745659344	40.0	38.0	41.0	34.0	41.0
16	38.261771036337855	40.0	37.0	41.0	34.0	41.0
17	38.16874639154277	40.0	37.0	41.0	34.0	41.0
18	38.14643312989561	40.0	37.0	41.0	34.0	41.0
19	38.0630664310063	40.0	37.0	41.0	34.0	41.0
20	38.120483206986634	40.0	37.0	41.0	34.0	41.0
21	38.10030681564295	40.0	37.0	41.0	34.0	41.0
22	38.2044316831946	40.0	37.0	41.0	34.0	41.0
23	38.252924924180924	40.0	37.0	41.0	34.0	41.0
24	38.22923132395263	40.0	37.0	41.0	34.0	41.0
25	37.85513965973769	40.0	37.0	41.0	33.0	41.0
26	37.987616770228925	40.0	37.0	41.0	34.0	41.0
27	38.04336121292829	40.0	37.0	41.0	34.0	41.0
28	37.95562565394646	40.0	37.0	41.0	33.0	41.0
29	37.97677093679421	40.0	37.0	41.0	34.0	41.0
30	37.707104983155006	40.0	37.0	41.0	33.0	41.0
31	37.66052076812303	40.0	37.0	41.0	33.0	41.0
32	37.69180844262857	40.0	37.0	41.0	33.0	41.0
33	37.71192953194577	40.0	37.0	41.0	33.0	41.0
34	37.62129835885706	40.0	37.0	41.0	33.0	41.0
35	37.694918628598224	40.0	37.0	41.0	33.0	41.0
36	37.53467547664817	40.0	37.0	41.0	33.0	41.0
37	37.51841225669873	40.0	37.0	41.0	33.0	41.0
38	37.37820171082349	40.0	37.0	41.0	32.0	41.0
39	37.35888803109301	40.0	36.0	41.0	32.0	41.0
40	37.31647137677713	40.0	36.0	41.0	32.0	41.0
41	37.16990330993689	40.0	36.0	41.0	32.0	41.0
42	37.24956256097048	40.0	36.0	41.0	32.0	41.0
43	37.2275235973747	40.0	36.0	41.0	32.0	41.0
44	37.21473555676974	40.0	36.0	41.0	32.0	41.0
45	37.20558638950766	40.0	36.0	41.0	32.0	41.0
46	37.08999630582463	40.0	36.0	41.0	32.0	41.0
47	37.04752434948226	39.0	36.0	41.0	32.0	41.0
48	37.0008958928291	39.0	35.0	41.0	32.0	41.0
49	36.99250325728936	39.0	35.0	41.0	32.0	41.0
50	36.92444858349389	39.0	35.0	41.0	31.0	41.0
51	35.75689671572325	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	21.0
10	13.0
11	21.0
12	14.0
13	17.0
14	14.0
15	17.0
16	25.0
17	57.0
18	87.0
19	182.0
20	355.0
21	586.0
22	995.0
23	1431.0
24	1922.0
25	2413.0
26	3110.0
27	3627.0
28	4112.0
29	4778.0
30	6100.0
31	7926.0
32	10736.0
33	14801.0
34	24781.0
35	29689.0
36	35032.0
37	55600.0
38	107332.0
39	136240.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.55062458108715	21.515142800892797	25.215733205327577	11.718499412692479
2	31.41420554214789	25.617004709520575	25.789768240267396	17.17902150806414
3	30.972010538354166	25.847282347814353	27.43577775664011	15.744929357191365
4	27.658976735543494	24.774644242063605	28.610614007339684	18.95576501505321
5	26.02424883257422	29.622419883954226	24.76889283130891	19.58443845216264
6	24.70385764815966	35.08272077121994	24.945859316068777	15.267562264551623
7	79.91894934997998	5.055047637165616	10.272240815992461	4.753762196861942
8	80.8205935898315	5.976379398446677	8.427807628582743	4.775219383139076
9	74.77785175959988	7.595401525893515	10.284628469925652	7.342118244580955
10	35.60521431747345	36.209333654822444	16.71581173420519	11.469640293498914
11	23.340773299296778	24.961122675379315	32.74410867511829	18.953995350205613
12	22.288486339293417	22.82270391516227	34.2684537332186	20.620356012325715
13	22.491555380555365	24.498134109626314	35.00684639087915	18.003464118939174
14	18.828570354132058	29.036218403187164	31.37062754527577	20.76458369740501
15	17.447125732475342	27.84457033643541	35.7363907243017	18.971913206787548
16	20.13148609817658	27.915799346551257	31.3294828375691	20.623231717703064
17	20.67233991722393	27.21678173174978	30.255738691288602	21.85513965973769
18	20.484313027166568	26.477283033559484	32.27735957156414	20.76104436770981
19	20.645573736403996	27.884830211718274	30.216584856535484	21.253011195342243
20	22.224114780462013	28.044100048002157	31.35182485627003	18.37996031526579
21	21.904026651152606	28.327688839829847	31.346294653621285	18.421989855396262
22	20.30712533430075	25.235199518651164	31.273738394869742	23.183936752178347
23	19.224532863782258	27.738832861791384	31.525694427546604	21.510939846879747
24	20.536960556382628	26.689642815271323	31.56927242441872	21.20412420392733
25	18.77260470332675	29.65936163764785	29.34679458394073	22.221239075084668
26	19.257714079674734	27.580447857931308	30.435138465213917	22.72669959718004
27	21.51005501445595	27.322740414499748	30.562775542346976	20.60442902869733
28	18.01032157022362	27.059723976525397	32.616029181773335	22.313925271477647
29	20.388087501078388	24.939886697208134	30.940598987309293	23.731426814404184
30	23.268659456757135	26.37574851292851	29.9933858776321	20.36220615268226
31	21.82903710323561	27.009509736474786	28.91057219900766	22.250880961281947
32	23.374618139507103	27.55213322036973	28.502443243530216	20.570805396592952
33	23.189024538615193	26.99468879337614	28.387636236542253	21.428650431466412
34	20.441619862718248	27.25527194218505	30.19424283783455	22.10886535726215
35	21.624419605232013	25.84197335327156	29.546103087401537	22.987503954094894
36	21.908450813271603	28.133246914699946	28.825185870111024	21.13311640191743
37	21.76223225523876	27.73529353209619	30.814952783129783	19.687521429535266
38	21.4414805016115	27.29442577693817	29.931005191754245	21.33308852969608
39	22.958746900321415	26.954207709987326	28.87429406963189	21.212751320059372
40	22.242696261361804	25.747075075819076	30.78309881587301	21.22712984694611
41	19.15883405631516	28.455325916962902	29.29458947093657	23.09125055578537
42	20.805285988899776	26.39654207488779	31.072439018455395	21.72573291775704
43	22.708339324386202	26.233290492696813	29.489695020384328	21.568675162532656
44	21.525981998084337	25.539581872438134	29.82681617385187	23.107619955625655
45	22.115501600440645	24.603429168058437	28.60088085067789	24.680188380823026
46	23.585871880689197	26.19856082006269	28.37856670419831	21.837000595049805
47	19.72202989406344	26.546963586933682	32.164985853741626	21.566020665261256
48	20.541163510395673	25.687348887212625	30.71563034355831	23.05585725883339
49	21.45585902849824	24.1119047566379	32.506088753116266	21.92614746174759
50	20.996631000546383	24.457874234343443	31.156940514928227	23.388554250181944
51	19.92023235699449	24.125619659206794	29.44766548025386	26.50648250354486
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	163.0
1	266.5
2	370.0
3	1476.0
4	2582.0
5	1721.5
6	861.0
7	913.0
8	965.0
9	940.5
10	916.0
11	880.5
12	845.0
13	835.5
14	826.0
15	865.0
16	904.0
17	937.0
18	970.0
19	1074.5
20	1179.0
21	1320.0
22	1461.0
23	1929.5
24	2398.0
25	3042.0
26	4200.0
27	4714.0
28	6158.5
29	7603.0
30	8693.0
31	9783.0
32	11067.5
33	12352.0
34	14125.0
35	15898.0
36	17298.5
37	18699.0
38	20008.0
39	21317.0
40	23788.0
41	26259.0
42	27808.5
43	29358.0
44	32424.5
45	35491.0
46	43465.5
47	51440.0
48	50332.5
49	49225.0
50	47108.5
51	44992.0
52	37744.0
53	30496.0
54	26351.0
55	22206.0
56	19269.0
57	16332.0
58	14124.0
59	11916.0
60	10827.5
61	9739.0
62	8386.0
63	7033.0
64	5574.5
65	4116.0
66	3236.0
67	2356.0
68	1710.0
69	1064.0
70	886.0
71	708.0
72	532.0
73	356.0
74	325.5
75	190.5
76	86.0
77	80.0
78	74.0
79	45.5
80	17.0
81	9.5
82	2.0
83	2.0
84	2.0
85	2.5
86	3.0
87	3.5
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	452063.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.61893310999914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.97495691669424	26.35646132927496
2	10.027718033445256	7.344086718253279
3	3.6244257067406407	3.981678075518906
4	1.9928084367925203	2.91898075391789
5	1.4735167037482626	2.697930480551202
6	1.2428765703945472	2.730768839715784
7	1.0733394405406647	2.751318162423775
8	0.9589061304393884	2.809129555946246
9	0.8604107975713939	2.83565928990491
>10	6.686137511203754	39.232795790265904
>50	0.053752015926468	1.3747075806694917
>100	0.02443273451203091	1.706194179197345
>500	0.0030540918140038635	0.6739332802728895
>1k	0.003664910176804637	2.586355964087428
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTC	2670	0.5906256428860579	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCG	2134	0.47205809809694665	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGC	2095	0.4634309819649031	No Hit
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1878	0.4154288229737891	RNA PCR Primer, Index 23 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1530	0.33844840210324667	No Hit
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1256	0.27783738107299205	RNA PCR Primer, Index 30 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	778	0.1720999064289712	RNA PCR Primer, Index 30 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCT	650	0.14378526886739237	RNA PCR Primer, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTG	553	0.12232808259025843	RNA PCR Primer, Index 30 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	520	0.11502821509391391	RNA PCR Primer, Index 23 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	512	0.11325855024631522	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCT	466	0.10308297737262286	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18006339824316522	0.0
2	0.0	0.0	0.0	1.1058193216432224	0.0
3	0.0	0.0	0.0	1.521911768934861	0.0
4	0.0	0.0	0.0	2.1811119246653674	0.0
5	0.0	0.0	0.0	4.107613319382476	0.0
6	0.0	0.0	0.0	4.957494862441739	0.0
7	0.0	0.0	0.0	5.8434333267708265	0.0
8	0.0	0.0	0.0	7.1885998190517695	0.0
9	0.0	0.0	0.0	7.648491471321475	0.0
10	0.0	0.0	0.0	9.068868719625362	0.0
11	0.0	0.0	0.0	10.516233356855128	0.0
12	0.0	0.0	0.0	12.214226778126058	0.0
13	0.0	0.0	0.0	12.743356567558061	0.0
14	0.0	0.0	0.0	12.951734603362805	0.0
15	0.0	0.0	0.0	13.379329872163835	0.0
16	0.0	0.0	0.0	14.092947221958001	0.0
17	0.0	0.0	0.0	15.057414563899279	0.0
18	0.0	0.0	0.0	16.041348219164142	0.0
19	0.0	0.0	0.0	16.761380604030855	0.0
20	0.0	0.0	0.0	17.36771202243935	0.0
21	0.0	0.0	0.0	18.09062011268341	0.0
22	0.0	0.0	0.0	18.911744601969193	0.0
23	0.0	0.0	0.0	19.658100751443936	0.0
24	0.0	0.0	0.0	20.251823307813293	0.0
25	0.0	0.0	0.0	20.79820732950938	0.0
26	0.0	0.0	0.0	21.300570938121457	0.0
27	0.0	0.0	0.0	21.823064484374964	0.0
28	0.0	0.0	0.0	22.332727960483385	0.0
29	0.0	0.0	0.0	22.850133720300047	0.0
30	0.0	0.0	0.0	23.468189168323885	0.0
31	0.0	0.0	0.0	24.01899735213897	0.0
32	0.0	0.0	0.0	24.550339222630473	0.0
33	0.0	0.0	0.0	25.05270283124255	0.0
34	0.0	0.0	0.0	25.5851507422638	0.0
35	0.0	0.0	0.0	26.147461747588277	0.0
36	0.0	0.0	0.0	26.660000929074045	0.0
37	0.0	0.0	0.0	27.159710040414723	0.0
38	0.0	0.0	0.0	27.656764654484	0.0
39	0.0	0.0	0.0	28.180806657479156	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	25	3.8870377E-5	45.0	1
CGATTAG	20	7.0287404E-4	45.0	10
CGCGAGG	25	3.8870377E-5	45.0	2
GCGTTAG	40	6.7993824E-9	45.0	1
CCGTCGC	25	3.8870377E-5	45.0	36
ATCCGTC	20	7.0287404E-4	45.0	28
TCGTGCG	20	7.0287404E-4	45.0	1
GCTTCGG	20	7.0287404E-4	45.0	2
TAACACG	20	7.0287404E-4	45.0	1
CGTAAGG	55	1.8189894E-12	45.0	2
TGGGCGC	20	7.0287404E-4	45.0	6
GCTACGC	20	7.0287404E-4	45.0	1
CGACTCA	20	7.0287404E-4	45.0	32
CCTATCG	25	3.8870377E-5	45.0	40
AAGTGCG	25	3.8870377E-5	45.0	1
GCCGTCG	25	3.8870377E-5	45.0	35
TATCCGG	20	7.0287404E-4	45.0	2
TCGCAAG	30	2.1625692E-6	44.999996	1
ATTAGCG	30	2.1625692E-6	44.999996	1
CGTTTTT	905	0.0	41.270714	1
>>END_MODULE