##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935524.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	537744
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14436981165759	31.0	31.0	33.0	28.0	34.0
2	31.154610744145913	31.0	31.0	34.0	28.0	34.0
3	30.95024584188759	31.0	30.0	34.0	27.0	34.0
4	34.803265122437445	35.0	35.0	37.0	32.0	37.0
5	34.81587521199679	35.0	35.0	37.0	32.0	37.0
6	34.97770500461186	36.0	35.0	37.0	32.0	37.0
7	35.438965381296676	37.0	35.0	37.0	33.0	37.0
8	35.13931164271475	37.0	35.0	37.0	32.0	37.0
9	37.01407733047696	39.0	37.0	39.0	33.0	39.0
10	36.373575530363894	38.0	35.0	39.0	32.0	39.0
11	36.5872943259246	38.0	35.0	39.0	32.0	39.0
12	36.626519310303784	39.0	35.0	39.0	33.0	39.0
13	36.61522211312446	39.0	35.0	39.0	33.0	39.0
14	37.49875033473177	39.0	36.0	41.0	32.0	41.0
15	37.73298446844595	40.0	36.0	41.0	33.0	41.0
16	37.73311464191139	39.0	36.0	41.0	33.0	41.0
17	37.66337699723288	39.0	36.0	41.0	33.0	41.0
18	37.65520396322414	39.0	36.0	41.0	33.0	41.0
19	37.530932934630606	39.0	36.0	41.0	33.0	41.0
20	37.58589031211878	39.0	36.0	41.0	33.0	41.0
21	37.504107902645124	39.0	35.0	41.0	33.0	41.0
22	37.63854733851052	39.0	35.0	41.0	33.0	41.0
23	37.709744785622895	39.0	35.0	41.0	33.0	41.0
24	37.69179014549674	40.0	35.0	41.0	33.0	41.0
25	37.26206708024636	39.0	35.0	41.0	32.0	41.0
26	37.373201746555985	39.0	35.0	41.0	33.0	41.0
27	37.43461014906721	39.0	35.0	41.0	33.0	41.0
28	37.327191005385465	39.0	35.0	41.0	33.0	41.0
29	37.29186750572763	39.0	35.0	41.0	33.0	41.0
30	37.01453107798506	39.0	35.0	41.0	32.0	41.0
31	37.004760629593264	39.0	35.0	41.0	32.0	41.0
32	36.99523565116487	39.0	35.0	41.0	32.0	41.0
33	36.98891108036538	39.0	35.0	41.0	32.0	41.0
34	36.856258740218394	39.0	35.0	41.0	31.0	41.0
35	36.867516885358086	39.0	35.0	41.0	31.0	41.0
36	36.70358758070755	39.0	35.0	41.0	31.0	41.0
37	36.72758412925109	39.0	35.0	41.0	31.0	41.0
38	36.585468178166565	39.0	35.0	41.0	31.0	41.0
39	36.46101118747954	39.0	35.0	41.0	31.0	41.0
40	36.35208761046148	39.0	35.0	41.0	30.0	41.0
41	36.1594736528906	39.0	35.0	40.0	30.0	41.0
42	36.199529887827666	39.0	35.0	40.0	30.0	41.0
43	36.167471882531466	39.0	35.0	40.0	30.0	41.0
44	36.17404750215716	38.0	35.0	40.0	30.0	41.0
45	36.15367349519474	38.0	35.0	40.0	30.0	41.0
46	36.04449700972954	38.0	35.0	40.0	30.0	41.0
47	36.0232527001696	38.0	35.0	40.0	30.0	41.0
48	35.93567943110477	38.0	35.0	40.0	30.0	41.0
49	35.89237071915261	38.0	35.0	40.0	30.0	41.0
50	35.76800299027046	38.0	35.0	40.0	29.0	41.0
51	34.61587855931447	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	14.0
9	38.0
10	44.0
11	25.0
12	24.0
13	25.0
14	38.0
15	45.0
16	69.0
17	141.0
18	223.0
19	414.0
20	701.0
21	1128.0
22	1560.0
23	2133.0
24	2890.0
25	4082.0
26	4926.0
27	5909.0
28	6475.0
29	7690.0
30	9390.0
31	12143.0
32	16137.0
33	23886.0
34	42232.0
35	42317.0
36	45330.0
37	68295.0
38	113566.0
39	125834.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.755683001576955	20.00635990359725	23.261812312178286	11.976144782647506
2	34.07829747984171	24.08320687910976	23.921047933589218	17.91744770745931
3	33.349140111279716	23.745313755244133	26.37909488529858	16.52645124817757
4	29.249047874081345	24.671962867089174	26.088808057365586	19.990181201463894
5	26.11595852301467	28.98330804248862	24.78354012318129	20.11719331131542
6	25.790524859412656	34.456172453807014	24.69650986342944	15.056792823350888
7	77.42345800232081	6.413274718081466	10.926574727007647	5.23669255259008
8	77.59510101460918	7.031226754738314	10.014802582641554	5.358869648010949
9	71.79457139456693	7.935560415364932	12.245231931922996	8.02463625814514
10	42.675325061739414	24.748951173792737	17.98922907554524	14.58649468892261
11	32.864336933559464	24.438580439763157	24.311754292005116	18.38532833467226
12	30.22534886488738	21.63873516022494	28.590370138950878	19.545545835936803
13	27.383848076408107	22.38481507929424	30.447573566604184	19.783763277693474
14	21.97848790502544	26.433953702877204	30.622191972388347	20.965366419709007
15	20.51403641881639	24.63644410723318	34.28397155523818	20.565547918712248
16	24.902369900919396	24.1747002291053	30.145943050968487	20.77698681900681
17	24.83560950935761	24.162240768841677	28.228673867118925	22.77347585468178
18	26.033577315599988	24.08320687910976	28.515799339462646	21.367416465827603
19	25.963841530542414	26.32423606771996	26.48825463417537	21.22366776756226
20	26.831540658752118	26.240739087744352	27.371388616144486	19.55633163735904
21	25.611257401291322	26.850694754381266	29.106972834657384	18.431075009670028
22	23.979254068850604	23.926254872206847	29.475735666041835	22.61875539290071
23	23.605656223035496	25.439986313189923	29.49377398911006	21.460583474664524
24	24.01030973846291	25.09800202326758	28.32500223154512	22.56668600672439
25	24.1817667886578	25.853566009104707	26.486209051147014	23.478458151090482
26	22.369938111815287	25.892804010830435	27.409882769496264	24.327375107858014
27	23.02192121158023	25.0721532922729	28.68874408640543	23.21718140974144
28	21.061322859948227	26.820568895236395	28.17753429141004	23.940573953405337
29	23.795523520485585	25.920140438573004	28.2984096521765	21.985926388764916
30	24.36363771608795	24.71770954208694	27.831086911244014	23.087565830581095
31	24.155360171382664	25.241564759439434	26.46110416852629	24.14197090065161
32	25.50470112172335	26.5048052604957	26.086390550150256	21.904103067630697
33	25.342542176202805	25.539290071111903	26.3943437769645	22.723823975720787
34	22.70466988009164	25.511581719182363	29.60349162426731	22.180256776458688
35	23.168273379154396	27.014341396649705	27.35465202773067	22.46273319646523
36	24.332024160195186	28.264192626974914	25.357233181588263	22.046550031241633
37	23.095748162694516	27.408023148561394	26.72907554524086	22.767153143503226
38	22.692396381921508	27.675250676902017	25.90842482668333	23.72392811449314
39	23.700682852807283	24.973035496444403	27.654794846618465	23.671486804129845
40	24.449552203278884	24.29167038590854	28.91264244696361	22.346134963848968
41	21.753473771906336	25.59340504031658	27.611279716742537	25.041841471034544
42	22.97766223333036	24.983263411586183	28.380604897497697	23.658469457585767
43	23.11341456157577	24.56763813264304	27.907145407480137	24.41180189830105
44	22.493230979797076	24.15517420928918	27.552143771013714	25.799451039900028
45	22.586212026540508	23.302166086464933	27.340332946532182	26.77128894046238
46	22.276027254604422	25.930926239995237	27.12796423577018	24.665082269630158
47	21.527715790413282	25.342170252015826	29.75151745068285	23.378596506888037
48	21.62925109345711	25.465091195810647	28.844580320747422	24.061077389984824
49	23.03624029277872	23.99747835401232	29.281777202535036	23.684504150673927
50	20.57689160641495	24.949232348478088	29.53877681573388	24.935099229373083
51	20.859553988514982	24.193854324734446	28.059634324139367	26.88695736261121
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	368.0
1	647.0
2	926.0
3	2545.0
4	4164.0
5	2806.0
6	1448.0
7	1511.5
8	1575.0
9	1462.5
10	1350.0
11	1325.0
12	1300.0
13	1336.0
14	1372.0
15	1365.5
16	1359.0
17	1374.5
18	1390.0
19	1393.5
20	1397.0
21	1429.5
22	1462.0
23	1768.0
24	2074.0
25	2617.0
26	3488.5
27	3817.0
28	4699.5
29	5582.0
30	6324.5
31	7067.0
32	8482.0
33	9897.0
34	11247.0
35	12597.0
36	13514.0
37	14431.0
38	16044.0
39	17657.0
40	19593.0
41	21529.0
42	23267.5
43	25006.0
44	27513.5
45	30021.0
46	37484.5
47	44948.0
48	46089.0
49	47230.0
50	48621.5
51	50013.0
52	45023.5
53	40034.0
54	37192.0
55	34350.0
56	32332.0
57	30314.0
58	29271.5
59	28229.0
60	26938.0
61	25647.0
62	22920.0
63	20193.0
64	17725.0
65	15257.0
66	13144.0
67	11031.0
68	9311.0
69	7591.0
70	6141.0
71	4691.0
72	3850.5
73	3010.0
74	2533.0
75	1562.0
76	1068.0
77	850.0
78	632.0
79	430.0
80	228.0
81	205.0
82	182.0
83	115.5
84	49.0
85	36.5
86	24.0
87	14.5
88	5.0
89	5.0
90	5.0
91	5.5
92	6.0
93	3.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	537744.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.48822795537669
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.99016171602321	24.828131078066406
2	9.134914480011716	6.300940258790308
3	3.5786044130069943	3.7025917427370665
4	2.0240572334355216	2.7922458904581346
5	1.440994808094865	2.484867871204499
6	1.2037119406998784	2.490833508207977
7	1.0607036172108846	2.5607251700423124
8	0.9646078383145995	2.661409201226964
9	0.8240814224789386	2.557899715702025
>10	7.678379160966243	44.28268759193361
>50	0.0659769821891688	1.5104336650274683
>100	0.028353744411872578	1.7251890491734774
>500	0.002726321578064671	0.6361799164327309
>1k	0.002726321578064671	1.4658653409970845
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2372	0.44110208575084053	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC	1784	0.33175637478056474	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	1252	0.23282454104555328	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	1244	0.23133684429765836	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG	1143	0.21255467285548513	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	809	0.1504433336308727	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	700	0.13017346544080455	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	696	0.1294296170668571	No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT	656	0.12199113332738254	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8596209348686364E-4	0.0	0.0	0.1277559582254753	0.0
2	1.8596209348686364E-4	0.0	0.0	0.6008435240560565	0.0
3	1.8596209348686364E-4	0.0	0.0	0.8556115921330596	0.0
4	1.8596209348686364E-4	0.0	0.0	1.258591448719093	0.0
5	1.8596209348686364E-4	0.0	0.0	2.4169493290487667	0.0
6	1.8596209348686364E-4	0.0	0.0	3.142573417834509	0.0
7	1.8596209348686364E-4	0.0	0.0	3.74788003213425	0.0
8	1.8596209348686364E-4	0.0	0.0	4.647750602517183	0.0
9	1.8596209348686364E-4	0.0	0.0	5.0085170638816985	0.0
10	1.8596209348686364E-4	0.0	0.0	5.935724062007201	0.0
11	1.8596209348686364E-4	0.0	0.0	7.028437323336012	0.0
12	3.719241869737273E-4	0.0	0.0	8.08637567318278	0.0
13	3.719241869737273E-4	0.0	0.0	8.520969085661578	0.0
14	3.719241869737273E-4	0.0	0.0	8.726271236871076	0.0
15	3.719241869737273E-4	0.0	0.0	9.061560891427892	0.0
16	3.719241869737273E-4	0.0	0.0	9.614612157457824	0.0
17	3.719241869737273E-4	0.0	0.0	10.226241482936118	0.0
18	3.719241869737273E-4	0.0	0.0	10.884919218066589	0.0
19	3.719241869737273E-4	0.0	0.0	11.351870034812103	0.0
20	3.719241869737273E-4	0.0	0.0	11.80152637686334	0.0
21	3.719241869737273E-4	0.0	0.0	12.315339641167544	0.0
22	7.438483739474546E-4	0.0	0.0	12.830454640126156	0.0
23	7.438483739474546E-4	0.0	0.0	13.331808444166741	0.0
24	7.438483739474546E-4	0.0	0.0	13.734230414472314	0.0
25	7.438483739474546E-4	0.0	0.0	14.110059805409266	0.0
26	7.438483739474546E-4	0.0	0.0	14.498906542890298	0.0
27	7.438483739474546E-4	0.0	0.0	14.90969680740278	0.0
28	7.438483739474546E-4	0.0	0.0	15.290733136957362	0.0
29	7.438483739474546E-4	0.0	0.0	15.691667410515041	0.0
30	7.438483739474546E-4	0.0	0.0	16.2259365051028	0.0
31	9.298104674343182E-4	0.0	0.0	16.656066827337916	0.0
32	9.298104674343182E-4	0.0	0.0	17.0880567705079	0.0
33	9.298104674343182E-4	0.0	0.0	17.514467850873277	0.0
34	9.298104674343182E-4	0.0	0.0	17.96338034455057	0.0
35	9.298104674343182E-4	0.0	0.0	18.425310184771938	0.0
36	9.298104674343182E-4	0.0	0.0	18.830707548573297	0.0
37	9.298104674343182E-4	0.0	0.0	19.257490553125653	0.0
38	9.298104674343182E-4	0.0	0.0	19.77855633907584	0.0
39	9.298104674343182E-4	0.0	0.0	20.549555178672378	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	20	7.0299325E-4	45.000004	1
GTATGCG	25	3.8880265E-5	45.0	1
TTATGCG	30	2.1633386E-6	44.999996	1
CGCATAC	30	2.1633386E-6	44.999996	26
TACGAAT	70	0.0	41.785713	12
GCTACGA	70	0.0	41.785713	10
ACGGGTA	55	6.002665E-11	40.909092	5
TGCACGG	105	0.0	40.714287	2
TACACGG	45	1.9250365E-8	40.0	2
CGCGACC	115	0.0	39.130436	10
CTACGAA	75	0.0	39.0	11
TATTAGG	105	0.0	38.57143	2
CGGGTCC	35	6.2420895E-6	38.571426	6
GTCGATG	35	6.2420895E-6	38.571426	1
CCCGATC	35	6.2420895E-6	38.571426	41
TTACACG	270	0.0	38.333336	34
CGTTTTT	1250	0.0	37.98	1
TACCCTT	30	1.139114E-4	37.499996	30
AACCCGC	30	1.139114E-4	37.499996	21
TTAGTCG	30	1.139114E-4	37.499996	1
>>END_MODULE