##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935522.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	509673
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.188681762620348	31.0	31.0	33.0	28.0	34.0
2	31.244680412735224	31.0	31.0	34.0	28.0	34.0
3	30.952905097974583	31.0	31.0	34.0	27.0	34.0
4	34.73093140111405	35.0	35.0	37.0	32.0	37.0
5	34.82384195356631	35.0	35.0	37.0	33.0	37.0
6	35.050265562429246	36.0	35.0	37.0	32.0	37.0
7	35.4721988412178	37.0	35.0	37.0	33.0	37.0
8	35.4206756096556	37.0	35.0	37.0	33.0	37.0
9	37.43087430568227	39.0	37.0	39.0	34.0	39.0
10	36.61655806762375	38.0	35.0	39.0	32.0	39.0
11	36.80237524844361	39.0	37.0	39.0	32.0	39.0
12	37.034765428029345	39.0	37.0	39.0	33.0	39.0
13	37.18149676361118	39.0	37.0	39.0	34.0	39.0
14	38.275109727217256	40.0	38.0	41.0	34.0	41.0
15	38.475950266151045	40.0	38.0	41.0	34.0	41.0
16	38.40235602042878	40.0	38.0	41.0	34.0	41.0
17	38.308805842177236	40.0	38.0	41.0	34.0	41.0
18	38.21304051813614	39.0	38.0	41.0	34.0	41.0
19	38.01362442193328	39.0	37.0	41.0	34.0	41.0
20	38.050750186884535	39.0	37.0	41.0	34.0	41.0
21	37.97511541714001	39.0	36.0	41.0	34.0	41.0
22	38.08367129512452	40.0	37.0	41.0	34.0	41.0
23	38.1329950772358	40.0	37.0	41.0	34.0	41.0
24	38.11321769055846	40.0	37.0	41.0	34.0	41.0
25	37.67180329348425	39.0	36.0	41.0	33.0	41.0
26	37.80448640598972	40.0	36.0	41.0	33.0	41.0
27	37.87534948878987	40.0	36.0	41.0	34.0	41.0
28	37.7936637805024	40.0	36.0	41.0	33.0	41.0
29	37.7521116480567	40.0	36.0	41.0	33.0	41.0
30	37.43748835037367	40.0	36.0	41.0	33.0	41.0
31	37.32458262454554	39.0	36.0	41.0	33.0	41.0
32	37.192423377341946	40.0	36.0	41.0	32.0	41.0
33	37.03673531852776	40.0	36.0	41.0	31.0	41.0
34	36.840852860559615	40.0	36.0	41.0	31.0	41.0
35	36.810505951855404	40.0	36.0	41.0	31.0	41.0
36	36.63933934110695	40.0	36.0	41.0	30.0	41.0
37	36.63321973108248	40.0	36.0	41.0	30.0	41.0
38	36.49241964946152	40.0	36.0	41.0	30.0	41.0
39	36.40205582795243	40.0	35.0	41.0	30.0	41.0
40	36.36066850706237	40.0	35.0	41.0	29.0	41.0
41	36.21475141904711	39.0	35.0	41.0	29.0	41.0
42	36.22606651715904	39.0	35.0	41.0	29.0	41.0
43	36.19566074718496	39.0	35.0	41.0	29.0	41.0
44	36.18155366283872	39.0	35.0	41.0	29.0	41.0
45	36.16056962012898	39.0	35.0	41.0	28.0	41.0
46	36.04375550598129	39.0	35.0	41.0	28.0	41.0
47	36.03829121809474	39.0	35.0	41.0	28.0	41.0
48	35.956595699595624	39.0	35.0	41.0	28.0	41.0
49	35.929541097919646	39.0	35.0	41.0	27.0	41.0
50	35.84790051660575	39.0	35.0	41.0	27.0	41.0
51	34.769081352161095	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	15.0
10	16.0
11	8.0
12	13.0
13	5.0
14	9.0
15	20.0
16	44.0
17	85.0
18	167.0
19	298.0
20	547.0
21	836.0
22	1369.0
23	2136.0
24	3738.0
25	5826.0
26	7519.0
27	7583.0
28	7126.0
29	7015.0
30	7570.0
31	9104.0
32	11511.0
33	16043.0
34	25782.0
35	32129.0
36	38368.0
37	60807.0
38	117205.0
39	146737.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.17624241425385	21.19986736593855	24.321672915771487	16.30221730403612
2	37.120663641197396	21.337406533208547	24.72173334667522	16.820196478918838
3	29.612712464658713	22.15126169131973	32.337989259780294	15.898036584241268
4	25.829502445685765	25.25423163479329	31.0742377956062	17.842028123914748
5	23.592185577811655	28.985447532045054	29.69099795358985	17.731368936553437
6	24.074259378071822	32.1015631591236	29.193424018929786	14.630753443874797
7	78.12911415750884	4.494450363272137	13.164323007104556	4.212112472114474
8	80.4313746264762	3.473207330974958	12.235884576973865	3.8595334655749864
9	74.14126312361063	6.923851175165254	14.085501880617574	4.849383820606546
10	31.918504609818456	36.745324943640334	19.99733162243242	11.338838824108793
11	21.21497509187263	25.080787092900742	36.0953003200091	17.60893749521752
12	20.614001526468932	23.364588667635918	37.134005529035285	18.88740427685987
13	20.65500821114715	24.501003584651336	37.97474066705515	16.869247537146368
14	18.282899035263785	27.629872486869033	35.42428184345649	18.66294663441069
15	17.083502559484216	27.90122294098373	37.61804137162455	17.3972331279075
16	20.59477351164374	27.995793381246408	34.016320268093466	17.39311283901639
17	20.65952090850408	26.494046182552346	32.801227453681086	20.04520545526249
18	20.940092961565554	26.072599490261407	34.68753494887899	18.299772599294055
19	19.85410253240803	27.921824385439294	33.041773843228896	19.18229923892378
20	20.784895413333647	27.522941179933014	33.37885271536848	18.313310691364855
21	20.639115668281427	27.36676261053656	34.53802732340148	17.45609439778054
22	20.74231909479215	24.44174990631248	34.00023152099483	20.815699477900537
23	18.412786237450288	26.64943993501716	34.15346702689764	20.784306800634916
24	18.486951437490312	26.045327101886894	35.48314311332953	19.984578347293265
25	18.878575086378913	28.248700637467554	32.461401722280755	20.411322553872775
26	18.928607165770995	28.574007255632534	32.08841747551862	20.408968103077857
27	19.931210795941713	27.172716624188446	33.01450145485439	19.88157112501545
28	18.17636013679359	26.790314574246626	33.985712407759486	21.047612881200298
29	18.487736254421954	25.117673488687846	33.162243242235704	23.232347014654493
30	20.438398738014374	25.364890822154596	32.40567972013429	21.791030719696746
31	19.65593625716881	26.361412121105097	32.30228008939065	21.68037153233544
32	19.31355987074065	26.874093781699248	31.247289929032927	22.565056418527174
33	19.49347915231923	26.378678093601195	30.657892413370924	23.46995034070865
34	17.988004073199875	26.231524918918602	32.63327663031002	23.147194377571502
35	18.185581735740367	27.34694598301264	30.72460185256037	23.742870428686626
36	19.42578869196524	28.32384685867213	30.264502926386132	21.985861522976496
37	18.657845324355026	27.760936914452994	32.64033998269479	20.940877778497192
38	18.33626658661534	28.563804635521205	30.703215591173166	22.396713186690288
39	19.26097713632074	26.937467748929215	30.420681495782592	23.380873618967453
40	19.99321133354131	26.665921090581605	31.65559093771889	21.68527663815819
41	18.82893541545265	28.08133842679522	30.663582336125316	22.426143821626805
42	20.23846662467896	27.980685655312325	30.424213171974973	21.35663454803374
43	20.484899141214072	27.034785048452637	30.634936518120444	21.84537929221285
44	20.175288861681903	26.256639060731096	29.954304034155232	23.61376804343177
45	20.08954761190018	25.06077426114391	29.89897444047458	24.950703686481333
46	20.905757220806283	25.491442552381628	30.14422973161223	23.45857049519986
47	18.94901240599365	26.282341815242322	31.862978811904892	22.905666966859144
48	19.029259937253887	26.27665189248793	31.74937656104993	22.944711609208255
49	20.119959268001246	25.07352753628307	31.852383783327742	22.954129412387942
50	19.08243128437253	24.733113192184007	31.946758019357507	24.237697504085954
51	18.471843711556232	24.644428878908634	30.71400682398322	26.169720585551914
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	152.0
1	266.0
2	380.0
3	4487.5
4	8595.0
5	5949.0
6	3303.0
7	3275.5
8	3248.0
9	3249.0
10	3250.0
11	3249.0
12	3248.0
13	3124.0
14	3000.0
15	2937.5
16	2875.0
17	2884.5
18	2894.0
19	2692.0
20	2490.0
21	2556.0
22	2622.0
23	2878.5
24	3135.0
25	3544.0
26	4795.0
27	5637.0
28	6839.5
29	8042.0
30	9189.5
31	10337.0
32	12235.5
33	14134.0
34	15641.5
35	17149.0
36	18470.5
37	19792.0
38	21395.5
39	22999.0
40	25452.5
41	27906.0
42	30579.5
43	33253.0
44	35526.0
45	37799.0
46	44248.5
47	50698.0
48	53335.0
49	55972.0
50	53038.5
51	50105.0
52	42771.5
53	35438.0
54	30525.0
55	25612.0
56	21533.5
57	17455.0
58	14765.0
59	12075.0
60	10224.5
61	8374.0
62	7221.5
63	6069.0
64	4837.5
65	3606.0
66	2763.5
67	1921.0
68	1436.0
69	951.0
70	761.5
71	572.0
72	441.0
73	310.0
74	235.5
75	129.5
76	98.0
77	64.0
78	30.0
79	21.0
80	12.0
81	14.0
82	16.0
83	8.5
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	509673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.529756940745635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.87038776490687	31.842193937008652
2	9.296812018696352	7.907823109579171
3	3.604428729216976	4.59886433291516
4	2.099046702328374	3.5708778422919765
5	1.5044116950591233	3.1991131864839826
6	1.2110577640860234	3.0903595408668867
7	0.9881159105338296	2.941703065300113
8	0.8459212750706105	2.8781460975806956
9	0.7347469888941847	2.8123749765552963
>10	4.778572699834134	31.73598639978772
>50	0.04789748595465342	1.3543538492992098
>100	0.013485699928710942	1.1186076769151514
>500	0.0027901448128367467	0.9075832088690999
>1k	0.0018600965418911644	1.008645536115147
>5k	4.650241354727911E-4	1.0333672404316947
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5225	1.0251671169553813	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTT	1512	0.29666080016010266	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTG	1309	0.2568313408793481	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTC	1208	0.23701471335542593	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	1071	0.21013473344673939	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCT	987	0.19365357788228924	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	945	0.18541300010006417	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCT	804	0.15774820325973712	No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGCT	765	0.15009623817624243	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCC	582	0.11419086355369032	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15539375246481568	0.0
2	0.0	0.0	0.0	0.7037845834486033	0.0
3	0.0	0.0	0.0	1.1795798482556463	0.0
4	0.0	0.0	0.0	1.7611291946012444	0.0
5	0.0	0.0	0.0	3.1845909043641707	0.0
6	0.0	0.0	0.0	4.44951959393572	0.0
7	0.0	0.0	0.0	5.446237097119133	0.0
8	0.0	0.0	0.0	6.78945127562182	0.0
9	0.0	0.0	0.0	7.360994206089003	0.0
10	0.0	0.0	0.0	8.42893384581879	0.0
11	0.0	0.0	0.0	9.85926270373357	0.0
12	0.0	0.0	0.0	11.031975403837363	0.0
13	0.0	0.0	0.0	11.552897642213733	0.0
14	0.0	0.0	0.0	11.790500968267889	0.0
15	0.0	0.0	0.0	12.136801439354253	0.0
16	0.0	0.0	0.0	12.791731168808235	0.0
17	0.0	0.0	0.0	13.703099830675747	0.0
18	0.0	0.0	0.0	14.660968895742958	0.0
19	0.0	0.0	0.0	15.310601110908367	0.0
20	0.0	0.0	0.0	15.90019483080328	0.0
21	0.0	0.0	0.0	16.590637526413996	0.0
22	0.0	0.0	0.0	17.359954323654577	0.0
23	0.0	0.0	0.0	18.12318878967495	0.0
24	1.962042329101208E-4	0.0	0.0	18.69375069897758	0.0
25	1.962042329101208E-4	0.0	0.0	19.20957162729829	0.0
26	1.962042329101208E-4	0.0	0.0	19.692037836024273	0.0
27	1.962042329101208E-4	0.0	0.0	20.18725731988942	0.0
28	3.924084658202416E-4	0.0	0.0	20.713673276787272	0.0
29	3.924084658202416E-4	0.0	0.0	21.2763870167735	0.0
30	5.886126987303624E-4	0.0	0.0	21.961335993862733	0.0
31	5.886126987303624E-4	0.0	0.0	22.530524473534992	0.0
32	7.848169316404832E-4	0.0	0.0	23.07832669182005	0.0
33	7.848169316404832E-4	0.0	0.0	23.608078120677376	0.0
34	7.848169316404832E-4	0.0	0.0	24.137437141068883	0.0
35	7.848169316404832E-4	0.0	0.0	24.681707683161555	0.0
36	7.848169316404832E-4	0.0	0.0	25.2234275702264	0.0
37	7.848169316404832E-4	0.0	0.0	25.763577823427962	0.0
38	7.848169316404832E-4	0.0	0.0	26.27861393481703	0.0
39	7.848169316404832E-4	0.0	0.0	26.85094168221585	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTACGA	30	2.163115E-6	45.000004	10
TTGCACG	35	1.2101555E-7	45.000004	1
TCTTGCG	20	7.029587E-4	45.0	1
GCGCTAG	20	7.029587E-4	45.0	1
CGTTTTT	3450	0.0	44.086956	1
CGACAGG	75	0.0	42.0	2
AGCGACT	120	0.0	41.250004	19
TAGGGCG	115	0.0	41.086956	5
TACGGGA	275	0.0	40.909092	4
GCGATCA	55	6.002665E-11	40.909092	9
CGTCAGG	55	6.002665E-11	40.909092	2
CTAACGG	50	1.0786607E-9	40.5	2
AAGGGCG	145	0.0	40.34483	5
CGTAAGG	125	0.0	39.600002	2
GGCGTTA	40	3.4531695E-7	39.375	9
TTCGTAG	40	3.4531695E-7	39.375	1
TAGTGCG	40	3.4531695E-7	39.375	1
TAACGGG	155	0.0	39.193546	3
CGTGGGT	35	6.2414456E-6	38.57143	4
CGAACGG	35	6.2414456E-6	38.57143	2
>>END_MODULE