FastQCFastQC Report
Sat 14 Jan 2017
SRR2935521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124661
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC16761.3444461379260555No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC14731.1816045114350118No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG13051.0468390274424237No Hit
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC11780.9449627389480272Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC6500.5214140749713222Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.4363834719759989No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT5250.42114213747683715Illumina Single End Adapter 1 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTT4190.3361115344815139No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGCAGAT3400.2727396699849993No Hit
GAACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT3370.27033314348513166No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCT3230.25910268648574936No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTA2870.23022436848733766No Hit
TCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC2740.2197960869879112Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTC2360.18931341798958776No Hit
ACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC1940.15562204699144078Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC1790.14358941449210258No Hit
CGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTG1660.13316113299267615No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG207.0090813E-445.02
AGATAAC253.8707563E-545.015
CGACGGG207.0090813E-445.03
CATGCGG207.0090813E-445.02
GGCACCG207.0090813E-445.08
TCGGGCA253.8707563E-545.05
AATGGGC207.0090813E-445.04
CAATTAC207.0090813E-445.027
AGGGTAT207.0090813E-445.06
TACGGGA406.73208E-945.04
CGACAGG207.0090813E-445.02
TGCGGGC207.0090813E-445.04
GGCTTCC207.0090813E-445.08
TCAATGG253.8707563E-545.02
TGACCCA207.0090813E-445.011
CGGGCAA207.0090813E-445.06
GGAGTTG207.0090813E-445.09
TCCAACC207.0090813E-445.036
AATTACC207.0090813E-445.028
TGGGCAC207.0090813E-445.06
Produced by FastQC (version 0.11.5)