##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935521.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	124661
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13601687777252	31.0	31.0	33.0	28.0	34.0
2	31.135335028597556	31.0	31.0	34.0	28.0	34.0
3	30.92854220646393	31.0	30.0	34.0	27.0	34.0
4	34.74257386030916	35.0	35.0	37.0	32.0	37.0
5	34.774532532227404	35.0	35.0	37.0	32.0	37.0
6	34.97162705256656	36.0	35.0	37.0	32.0	37.0
7	35.38854974691363	37.0	35.0	37.0	33.0	37.0
8	35.186465694964745	37.0	35.0	37.0	32.0	37.0
9	36.95081862009771	39.0	37.0	39.0	32.0	39.0
10	36.41160427078236	37.0	35.0	39.0	32.0	39.0
11	36.6254562373156	38.0	35.0	39.0	32.0	39.0
12	36.51741924098154	38.0	35.0	39.0	33.0	39.0
13	36.39666776297318	39.0	35.0	39.0	32.0	39.0
14	37.37727918113925	39.0	36.0	41.0	32.0	41.0
15	37.60643665621165	39.0	36.0	41.0	33.0	41.0
16	37.62811945997545	39.0	36.0	41.0	33.0	41.0
17	37.51290299291679	39.0	35.0	41.0	33.0	41.0
18	37.53833997802039	39.0	36.0	40.0	33.0	41.0
19	37.41751630421704	39.0	36.0	40.0	33.0	41.0
20	37.42763173727148	39.0	35.0	40.0	33.0	41.0
21	37.43289400855119	39.0	35.0	40.0	33.0	41.0
22	37.57183080514355	39.0	35.0	41.0	33.0	41.0
23	37.62879328739542	39.0	35.0	41.0	33.0	41.0
24	37.64102646376974	39.0	35.0	41.0	33.0	41.0
25	37.287347285839196	39.0	35.0	41.0	33.0	41.0
26	37.373027650989485	39.0	35.0	41.0	33.0	41.0
27	37.36337747972502	39.0	35.0	41.0	33.0	41.0
28	37.24819309968635	39.0	35.0	41.0	33.0	41.0
29	37.22210635242779	39.0	35.0	41.0	33.0	41.0
30	37.01421454985922	39.0	35.0	41.0	32.0	41.0
31	36.98434955599586	39.0	35.0	41.0	32.0	41.0
32	36.9599473772872	39.0	35.0	41.0	32.0	41.0
33	36.86448047103745	39.0	35.0	41.0	32.0	41.0
34	36.71332654158077	39.0	35.0	41.0	31.0	41.0
35	36.8254947417396	39.0	35.0	41.0	32.0	41.0
36	36.675576162552844	39.0	35.0	41.0	31.0	41.0
37	36.64012802720979	39.0	35.0	41.0	31.0	41.0
38	36.49977940173751	39.0	35.0	41.0	31.0	41.0
39	36.44982793335526	39.0	35.0	40.0	31.0	41.0
40	36.359366602225236	39.0	35.0	40.0	30.0	41.0
41	36.15582259086643	38.0	35.0	40.0	30.0	41.0
42	36.2782105068947	39.0	35.0	40.0	30.0	41.0
43	36.21364340090325	38.0	35.0	40.0	30.0	41.0
44	36.2126888120583	38.0	35.0	40.0	30.0	41.0
45	36.22922164911239	38.0	35.0	40.0	30.0	41.0
46	36.13243917504271	38.0	35.0	40.0	30.0	41.0
47	36.05214942925213	38.0	35.0	40.0	30.0	41.0
48	36.0508819919622	38.0	35.0	40.0	30.0	41.0
49	36.045667851212485	38.0	35.0	40.0	30.0	41.0
50	35.888040365471156	38.0	35.0	40.0	30.0	41.0
51	34.67107595799809	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	2.0
11	9.0
12	4.0
13	4.0
14	5.0
15	10.0
16	9.0
17	18.0
18	32.0
19	67.0
20	141.0
21	270.0
22	419.0
23	519.0
24	717.0
25	921.0
26	1117.0
27	1328.0
28	1417.0
29	1608.0
30	2019.0
31	2789.0
32	3763.0
33	5564.0
34	11044.0
35	11895.0
36	9737.0
37	14491.0
38	25767.0
39	28958.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28218127561948	20.674469160362904	23.585564049702796	12.457785514314821
2	30.67599329381282	27.72478962947514	24.90674709813013	16.692469978581915
3	29.139025035897355	28.007155405459606	27.140805865507257	15.713013693135785
4	28.01437498495921	23.449194214710296	31.75491934125348	16.781511459077016
5	27.107114494509112	27.894850835465785	24.447902712155365	20.550131957869745
6	21.965169539791916	36.12517146501312	26.190227898059536	15.71943109713543
7	73.68623707494726	5.218151627213001	15.491613255147962	5.603998042691781
8	73.21536005647316	8.099566023054523	11.394100801373325	7.290973119098997
9	68.61007051122645	6.376493049149293	13.264774067270437	11.748662372353824
10	42.305131516673214	24.767168561137805	18.262327431995573	14.665372490193404
11	32.45762507921483	22.31251153127281	26.06428634456646	19.165577044945895
12	27.590024145482552	18.624108582475674	32.56832529820875	21.217541973833036
13	23.48288558570844	24.96931678712669	34.0836348176254	17.46416280953947
14	17.53475425353559	30.72091512181035	29.880235197856585	21.864095426797476
15	15.61434610664121	24.288269787664145	40.96630060724685	19.13108349844779
16	17.744924234524028	24.312335052662824	33.49724452715765	24.445496185655497
17	17.55240211453462	23.99948660768003	30.72171729731031	27.726393980475052
18	20.850947770353198	22.966284563736856	32.598807967207065	23.583959698702884
19	20.51964928887142	26.81913349002495	28.307169042443103	24.354048178660527
20	24.568229037148747	25.503565670097306	29.919541797354427	20.008663495399524
21	21.703660326806297	29.329942804886855	29.833709018859146	19.132687849447702
22	18.19494468999928	24.062056296676587	31.376292505274307	26.36670650804983
23	19.063700756451496	27.146421094006946	28.263049389945532	25.526828759596025
24	22.79542118224625	23.098643521229576	29.728624028364926	24.37731126815925
25	16.860124658072692	28.83580269691403	27.367019356494815	26.937053288518463
26	16.548078388589857	26.44130882954573	32.1255244222331	24.885088359631318
27	20.054387498897007	27.78575496747178	28.62563271592559	23.534224817705617
28	17.84439399651856	26.085142907565317	33.518101090156506	22.552362005759623
29	20.898276124850597	22.298072372273605	33.568638146653726	23.235013356222076
30	20.279798814384613	27.64938513247928	30.144150937342072	21.926665115794034
31	26.6145787375362	26.739718115529314	24.254578416665996	22.391124730268487
32	29.510432292376926	27.061390491011622	23.93531256768356	19.492864648927892
33	23.88798421318616	27.518630525986477	23.067358676731295	25.52602658409607
34	22.29486367027378	26.809507384025476	29.100520611899473	21.79510833380127
35	22.454496594765004	28.163579627951002	25.985673145570786	23.396250631713205
36	24.417420043157044	28.9545246709075	27.135190637007568	19.492864648927892
37	23.918466882184482	28.73713511041946	28.664137139923472	18.680260867472587
38	24.602722583646848	27.3581954259953	23.0272499017335	25.01183208862435
39	25.065577847121396	26.23835842805689	26.933042411018683	21.763021313803034
40	24.123021634673233	23.698670795196573	27.689493907477075	24.488813662653115
41	19.373340499434466	28.9745790584064	25.697692141086627	25.954388301072505
42	24.654863991143984	24.54657029864994	27.19695815050417	23.60160755970191
43	25.42816117310145	24.91797755512951	25.894225138575816	23.759636133193222
44	21.93388469529364	25.29500004010877	26.91860325201948	25.85251201257811
45	21.5472361043149	23.604816261701735	26.024979745068627	28.822967888914736
46	25.86294029407754	25.7963597275812	26.11161469906386	22.2290852792774
47	19.437514539430936	24.79604687913622	31.968298024241744	23.798140557191104
48	22.480166210763592	23.654551142699	28.433912771436137	25.43136987510127
49	22.793816831246343	20.627942981365464	33.21568092667314	23.36255926071506
50	21.276902960829773	22.254754895275987	30.903008960300333	25.565333183593907
51	20.218031300888008	22.272402756275017	27.07021442151114	30.439351521325836
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	147.5
2	214.0
3	586.5
4	959.0
5	629.5
6	300.0
7	307.0
8	314.0
9	295.0
10	276.0
11	299.0
12	322.0
13	308.5
14	295.0
15	286.5
16	278.0
17	283.5
18	289.0
19	257.5
20	226.0
21	277.5
22	329.0
23	372.0
24	415.0
25	541.5
26	697.5
27	727.0
28	716.5
29	706.0
30	952.5
31	1199.0
32	1426.5
33	1654.0
34	2152.0
35	2650.0
36	2609.5
37	2569.0
38	3006.5
39	3444.0
40	4108.5
41	4773.0
42	6061.5
43	7350.0
44	8110.5
45	8871.0
46	13537.5
47	18204.0
48	16947.0
49	15690.0
50	15327.5
51	14965.0
52	12347.0
53	9729.0
54	8257.0
55	6785.0
56	6004.5
57	5224.0
58	4731.0
59	4238.0
60	4088.5
61	3939.0
62	3268.5
63	2598.0
64	2236.5
65	1875.0
66	1446.0
67	1017.0
68	829.0
69	641.0
70	473.5
71	306.0
72	295.0
73	284.0
74	192.0
75	90.0
76	80.0
77	66.5
78	53.0
79	30.0
80	7.0
81	11.5
82	16.0
83	8.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	124661.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.83795252725391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.24799151427057	30.729739052309863
2	10.512875915849849	8.586486551527743
3	3.545542045610796	4.343780332261092
4	1.756074564418865	2.868579587842228
5	1.1255377241745075	2.2982328073735974
6	0.8878587283191577	2.1754999558803476
7	0.7307155905635546	2.0888650018851123
8	0.6266082618004676	2.0471518758874065
9	0.5833938989176766	2.144215111382068
>10	4.889115873421203	32.8290323356944
>50	0.06089296588029622	1.7816317854020103
>100	0.019642892219450394	2.2099935023784503
>500	0.0058928676658351175	1.3789396844241584
>1k	0.007857156887780157	4.517852415751518
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC	1676	1.3444461379260555	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC	1473	1.1816045114350118	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG	1305	1.0468390274424237	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	1178	0.9449627389480272	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	650	0.5214140749713222	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	544	0.4363834719759989	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	525	0.42114213747683715	Illumina Single End Adapter 1 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTT	419	0.3361115344815139	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGCAGAT	340	0.2727396699849993	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT	337	0.27033314348513166	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCT	323	0.25910268648574936	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTA	287	0.23022436848733766	No Hit
TCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	274	0.2197960869879112	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTC	236	0.18931341798958776	No Hit
ACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	194	0.15562204699144078	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC	179	0.14358941449210258	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTG	166	0.13316113299267615	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4387899984758665	0.0
2	0.0	0.0	0.0	2.386472112368744	0.0
3	0.0	0.0	0.0	3.0193885818339337	0.0
4	0.0	0.0	0.0	4.1239842452731805	0.0
5	0.0	0.0	0.0	7.368784142594717	0.0
6	0.0	0.0	0.0	8.771789092017551	0.0
7	0.0	0.0	0.0	10.084148209945372	0.0
8	0.0	0.0	0.0	12.151354473331676	0.0
9	0.0	0.0	0.0	12.83801670129391	0.0
10	0.0	0.0	0.0	15.344012963156079	0.0
11	0.0	0.0	0.0	17.493041127537882	0.0
12	0.0	0.0	0.0	20.491573146372964	0.0
13	0.0	0.0	0.0	21.386801004323726	0.0
14	0.0	0.0	0.0	21.848051916798358	0.0
15	0.0	0.0	0.0	22.578031621758207	0.0
16	0.0	0.0	0.0	23.59438798020231	0.0
17	0.0	0.0	0.0	24.791233826136484	0.0
18	0.0	0.0	0.0	26.260819342055655	0.0
19	0.0	0.0	0.0	27.351778021995653	0.0
20	0.0	0.0	0.0	28.112240395953826	0.0
21	0.0	0.0	0.0	29.038753098402868	0.0
22	0.0	0.0	0.0	29.93077225435381	0.0
23	0.0	0.0	0.0	30.797121794306157	0.0
24	0.0	0.0	0.0	31.48217967126848	0.0
25	0.0	0.0	0.0	32.065361259736406	0.0
26	0.0	0.0	0.0	32.615653652706136	0.0
27	0.0	0.0	0.0	33.151506886676664	0.0
28	0.0	0.0	0.0	33.72746889564499	0.0
29	0.0	0.0	0.0	34.27294823561499	0.0
30	0.0	0.0	0.0	34.8817994400815	0.0
31	0.0	0.0	0.0	35.47781583654872	0.0
32	0.0	0.0	0.0	36.023295176518715	0.0
33	0.0	0.0	0.0	36.596048483487216	0.0
34	0.0	0.0	0.0	37.160780035456156	0.0
35	0.0	0.0	0.0	37.74957685242377	0.0
36	0.0	0.0	0.0	38.26216699689558	0.0
37	0.0	0.0	0.0	38.78438324736686	0.0
38	0.0	0.0	0.0	39.380399643834075	0.0
39	0.0	0.0	0.0	39.97400951380143	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGCG	20	7.0090813E-4	45.0	2
AGATAAC	25	3.8707563E-5	45.0	15
CGACGGG	20	7.0090813E-4	45.0	3
CATGCGG	20	7.0090813E-4	45.0	2
GGCACCG	20	7.0090813E-4	45.0	8
TCGGGCA	25	3.8707563E-5	45.0	5
AATGGGC	20	7.0090813E-4	45.0	4
CAATTAC	20	7.0090813E-4	45.0	27
AGGGTAT	20	7.0090813E-4	45.0	6
TACGGGA	40	6.73208E-9	45.0	4
CGACAGG	20	7.0090813E-4	45.0	2
TGCGGGC	20	7.0090813E-4	45.0	4
GGCTTCC	20	7.0090813E-4	45.0	8
TCAATGG	25	3.8707563E-5	45.0	2
TGACCCA	20	7.0090813E-4	45.0	11
CGGGCAA	20	7.0090813E-4	45.0	6
GGAGTTG	20	7.0090813E-4	45.0	9
TCCAACC	20	7.0090813E-4	45.0	36
AATTACC	20	7.0090813E-4	45.0	28
TGGGCAC	20	7.0090813E-4	45.0	6
>>END_MODULE