FastQCFastQC Report
Sat 14 Jan 2017
SRR2935520.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935520.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445999
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38380.860540046053915No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC26890.6029161500362108No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC19590.43923865300146414No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG18850.4226466875486268No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC15210.3410321547806161No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG11210.2513458550355494No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC10290.23071800609418408No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT9520.21345339339325872No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC8410.18856544521400273No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT8050.18049367823694673Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT7100.1591931820474934No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT6680.14977612057426137No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT5710.1280271928860827No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC5180.11614375816986137No Hit
CGTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT4900.10986571718770669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG302.162502E-645.0000042
TTGTGCG351.2097007E-745.01
GTCCTCG207.028638E-445.09
TAGCCGT207.028638E-445.044
ATACGCG207.028638E-445.01
GTAAGCG253.8869526E-545.01
TAAGCGG551.8189894E-1245.02
CGATAGA253.8869526E-545.010
GGCTACG253.8869526E-545.01
CGTTTTT21650.043.337181
CACAACG1250.043.212
ATGACGG603.6379788E-1241.2500042
CGACAGG556.002665E-1140.9090922
ACAACGA1350.040.00000413
CCGATGA2250.040.018
GCGATAT451.9237632E-840.09
AACGAGC1300.039.8076915
CGTATGG403.451878E-739.3752
CGAATAT800.039.37514
GCTACGA800.039.37510
Produced by FastQC (version 0.11.5)