##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935520.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445999
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.193406263242743	31.0	31.0	33.0	28.0	34.0
2	31.18664167408447	31.0	31.0	34.0	28.0	34.0
3	30.965105302926688	31.0	31.0	34.0	27.0	34.0
4	34.77226630552983	35.0	35.0	37.0	32.0	37.0
5	34.81707133872497	35.0	35.0	37.0	33.0	37.0
6	35.025816201381616	36.0	35.0	37.0	32.0	37.0
7	35.42831486169251	37.0	35.0	37.0	33.0	37.0
8	35.18329637510398	37.0	35.0	37.0	32.0	37.0
9	37.063015836358375	39.0	37.0	39.0	33.0	39.0
10	36.450891145495845	38.0	35.0	39.0	32.0	39.0
11	36.740550987782484	39.0	37.0	39.0	32.0	39.0
12	36.89067015845327	39.0	37.0	39.0	33.0	39.0
13	36.9617241294263	39.0	37.0	39.0	33.0	39.0
14	38.05392837203671	40.0	37.0	41.0	33.0	41.0
15	38.2074219897354	40.0	37.0	41.0	34.0	41.0
16	38.1477940533499	40.0	37.0	41.0	34.0	41.0
17	38.03920412377606	40.0	37.0	41.0	33.0	41.0
18	37.97680712288592	39.0	37.0	41.0	33.0	41.0
19	37.81369464953957	39.0	37.0	41.0	33.0	41.0
20	37.852241821170004	39.0	36.0	41.0	34.0	41.0
21	37.81194352453705	39.0	36.0	41.0	33.0	41.0
22	37.932807024230996	40.0	36.0	41.0	33.0	41.0
23	37.968634458821654	40.0	36.0	41.0	34.0	41.0
24	37.95244159740268	40.0	36.0	41.0	34.0	41.0
25	37.55691828905446	39.0	36.0	41.0	33.0	41.0
26	37.65288711409667	39.0	36.0	41.0	33.0	41.0
27	37.69988721947807	40.0	36.0	41.0	33.0	41.0
28	37.58493853125231	40.0	36.0	41.0	33.0	41.0
29	37.51643165119204	40.0	36.0	41.0	33.0	41.0
30	37.246063331980565	39.0	35.0	41.0	32.0	41.0
31	37.21174486938312	39.0	35.0	41.0	32.0	41.0
32	37.15610797333626	39.0	35.0	41.0	32.0	41.0
33	37.05661671887157	40.0	35.0	41.0	32.0	41.0
34	36.89943475209586	40.0	35.0	41.0	31.0	41.0
35	36.88444144493597	40.0	35.0	41.0	31.0	41.0
36	36.727685488084056	40.0	35.0	41.0	31.0	41.0
37	36.7343872968325	40.0	35.0	41.0	31.0	41.0
38	36.578907127594455	39.0	35.0	41.0	30.0	41.0
39	36.47893829358362	39.0	35.0	41.0	30.0	41.0
40	36.390525539294934	39.0	35.0	41.0	30.0	41.0
41	36.21236146269386	39.0	35.0	41.0	30.0	41.0
42	36.26099385873062	39.0	35.0	41.0	30.0	41.0
43	36.2152695409631	39.0	35.0	41.0	30.0	41.0
44	36.21112154959989	39.0	35.0	41.0	30.0	41.0
45	36.17800264126153	39.0	35.0	41.0	30.0	41.0
46	36.048870064731084	39.0	35.0	41.0	29.0	41.0
47	36.01153141598972	39.0	35.0	41.0	29.0	41.0
48	35.955789138540666	39.0	35.0	40.0	29.0	41.0
49	35.9186993692811	39.0	35.0	40.0	29.0	41.0
50	35.799672196574434	39.0	35.0	40.0	28.0	41.0
51	34.63158886006471	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	19.0
10	24.0
11	13.0
12	16.0
13	23.0
14	19.0
15	25.0
16	46.0
17	80.0
18	151.0
19	265.0
20	523.0
21	872.0
22	1312.0
23	1859.0
24	2678.0
25	3992.0
26	5042.0
27	5711.0
28	5783.0
29	6216.0
30	7198.0
31	9035.0
32	11558.0
33	16284.0
34	28361.0
35	32388.0
36	35825.0
37	55327.0
38	98226.0
39	117097.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58798562328615	20.44690683162967	23.329424505436112	14.63568303964807
2	34.50949441590676	24.96889006477593	23.962161350137556	16.55945416917975
3	30.90298408740827	24.410368633113528	29.120020448476343	15.566626831001864
4	27.411720654082185	24.488619929641096	29.6361651035092	18.463494312767516
5	25.317993986533605	28.628315310124012	27.045800551122312	19.00789015222007
6	24.22404534539315	34.41375429092891	26.652301910990833	14.709898452687115
7	75.35218688831141	5.611895990798186	13.917295778690086	5.118621342200319
8	76.15196446628804	7.007639030580787	11.609667286249522	5.230729216881652
9	69.91002221978077	7.630510382310273	14.014605413913483	8.44486198399548
10	36.324296691248186	29.22988616566405	20.450270067870107	13.995547075217656
11	27.117549590918365	25.009473115410575	29.162845656604613	18.710131637066453
12	25.57359994080704	21.12381417895556	32.92541014665952	20.37717573357788
13	23.60520987715219	22.702741485967458	34.42496507839704	19.267083558483314
14	18.94690346839343	28.36306807862798	31.278769683340098	21.411258769638497
15	17.790847064679518	25.636828782127314	36.65008217507214	19.92224197812103
16	20.307220419776726	25.91059621209913	32.921822694669714	20.860360673454426
17	20.333229446702795	25.345348307955845	31.060831974959584	23.260590270381773
18	21.5848017596452	25.617546227682126	31.30926302525342	21.488388987419256
19	22.09018405870865	26.973378864078168	29.129437509949575	21.806999567263606
20	24.312610566391406	26.06328713741511	30.008811678949954	19.615290617243534
21	23.133908371991865	27.351406617503628	30.231009486568354	19.283675523936154
22	20.91193029580784	25.377859591613433	29.858362911127607	23.851847201451122
23	20.552064018080756	26.815306760777492	30.73908237462416	21.89354684651759
24	21.771573478864305	24.482341888658944	30.87787192347965	22.868212708997106
25	21.05744631714421	27.43481487626654	28.42203682071036	23.08570198587889
26	20.146233511734334	27.378088291677784	29.28033470927065	23.195343487317235
27	21.40811974914742	27.495801560093184	28.958136677436496	22.1379420133229
28	18.991073971017872	28.048717598021522	30.88639212195543	22.073816309005174
29	20.81349958183763	27.2296574655997	29.63751039800538	22.3193325545573
30	21.973816084789426	27.87898627575398	29.21486371045675	20.932333928999842
31	22.145116917302506	27.56104834315772	28.22517539276994	22.068659346769838
32	24.527633470030203	28.709929842892024	26.66687593469941	20.09556075237837
33	24.323597138110177	27.05835663308662	26.981002199556503	21.637044029246702
34	22.622472247695622	26.546472077291654	28.233247159746995	22.59780851526573
35	23.019109908318182	27.14804293283169	27.033244469158003	22.79960268969213
36	21.87493693932049	31.118455422545793	26.575395908959436	20.43121172917428
37	22.070453072764735	29.336612862360678	28.065085347724995	20.527848717149592
38	23.59242957943852	27.166877055778155	26.903199334527656	22.337494030255673
39	24.87292572404871	25.3684425301402	26.787055576357794	22.971576169453297
40	24.6940015560573	24.256332413301376	28.837284388530023	22.212381642111307
41	20.530539306141943	27.701183186509386	27.886161179733588	23.882116327615087
42	22.197134971154643	28.093560747894053	28.10521996686091	21.60408431409039
43	22.45677680891661	27.2836934611961	27.955219630537286	22.30431009935
44	22.128524951849666	25.87606698669728	28.057686227996026	23.937721833457026
45	22.52561104397095	24.412835006356516	27.320913275590303	25.740640674082226
46	23.24556781517447	26.77898380938074	26.87158491386752	23.10386346157727
47	21.01036100977805	25.974946132166217	30.079215424249828	22.935477433805907
48	21.398478471924825	25.205437680353544	29.226747145172972	24.169336702548662
49	22.059242285296605	24.307005172657338	30.52899221747134	23.10476032457472
50	20.707445532389084	23.926959477487618	30.388184726871586	24.977410263251713
51	20.242422068210917	23.200724665301937	28.792665454406848	27.764187812080298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	341.0
1	487.0
2	633.0
3	3469.0
4	6305.0
5	4195.5
6	2086.0
7	2155.0
8	2224.0
9	2052.0
10	1880.0
11	1889.0
12	1898.0
13	1858.0
14	1818.0
15	1726.5
16	1635.0
17	1634.5
18	1634.0
19	1643.0
20	1652.0
21	1726.5
22	1801.0
23	1956.0
24	2111.0
25	2449.5
26	3248.5
27	3709.0
28	4509.5
29	5310.0
30	5969.5
31	6629.0
32	7912.5
33	9196.0
34	10321.0
35	11446.0
36	12107.0
37	12768.0
38	14378.5
39	15989.0
40	18098.5
41	20208.0
42	22500.5
43	24793.0
44	27259.0
45	29725.0
46	38211.5
47	46698.0
48	45755.5
49	44813.0
50	43394.0
51	41975.0
52	36165.0
53	30355.0
54	26854.0
55	23353.0
56	21473.0
57	19593.0
58	18324.0
59	17055.0
60	16809.5
61	16564.0
62	14927.5
63	13291.0
64	11058.0
65	8825.0
66	7220.0
67	5615.0
68	4526.5
69	3438.0
70	2741.0
71	2044.0
72	1869.0
73	1694.0
74	1359.0
75	745.5
76	467.0
77	429.5
78	392.0
79	258.5
80	125.0
81	89.5
82	54.0
83	36.5
84	19.0
85	14.5
86	10.0
87	7.5
88	5.0
89	6.5
90	8.0
91	4.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	445999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.14728630516882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.00749900729676	30.45207750382737
2	9.36412894070832	7.706169890436847
3	3.688871179722944	4.553611157248374
4	2.2308421682657875	3.6717240559710382
5	1.6125330044475374	3.317567860526844
6	1.2996888916551272	3.208720255954863
7	1.1291537787943242	3.2523129673029323
8	0.9230408219237031	3.038449997684236
9	0.8290842688957292	3.070311100502772
>10	4.821471444529599	29.840930732154835
>50	0.060069575410936625	1.6215712684691144
>100	0.02590156003957786	1.933821811984963
>500	0.0038576791548307447	1.1485468430703374
>1k	0.0038576791548307447	3.1841845548654844
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3838	0.860540046053915	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	2689	0.6029161500362108	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	1959	0.43923865300146414	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	1885	0.4226466875486268	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1521	0.3410321547806161	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	1121	0.2513458550355494	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1029	0.23071800609418408	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT	952	0.21345339339325872	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	841	0.18856544521400273	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	805	0.18049367823694673	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT	710	0.1591931820474934	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	668	0.14977612057426137	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT	571	0.1280271928860827	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC	518	0.11614375816986137	No Hit
CGTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	490	0.10986571718770669	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.726472480880002E-4	0.0	0.0	0.18834122946464005	0.0
2	6.726472480880002E-4	0.0	0.0	0.9464146780598163	0.0
3	6.726472480880002E-4	0.0	0.0	1.443052562898123	0.0
4	6.726472480880002E-4	0.0	0.0	2.1201841259733767	0.0
5	8.968629974506669E-4	0.0	0.0	4.043506824006331	0.0
6	8.968629974506669E-4	0.0	0.0	5.281401976237615	0.0
7	8.968629974506669E-4	0.0	0.0	6.280283139648295	0.0
8	8.968629974506669E-4	0.0	0.0	7.655174114740167	0.0
9	8.968629974506669E-4	0.0	0.0	8.208314368417867	0.0
10	8.968629974506669E-4	0.0	0.0	9.683878214973577	0.0
11	8.968629974506669E-4	0.0	0.0	11.179397263222564	0.0
12	8.968629974506669E-4	0.0	0.0	12.799580268117193	0.0
13	0.0011210787468133336	0.0	0.0	13.393527788178897	0.0
14	0.0011210787468133336	0.0	0.0	13.67379747488223	0.0
15	0.0011210787468133336	0.0	0.0	14.109448675893892	0.0
16	0.0011210787468133336	0.0	0.0	14.844652118054077	0.0
17	0.0011210787468133336	0.0	0.0	15.728959033540434	0.0
18	0.0011210787468133336	0.0	0.0	16.692862540050537	0.0
19	0.0011210787468133336	0.0	0.0	17.334119583227764	0.0
20	0.0011210787468133336	0.0	0.0	17.92829131903883	0.0
21	0.0011210787468133336	0.0	0.0	18.60609552936217	0.0
22	0.0011210787468133336	0.0	0.0	19.36013309446882	0.0
23	0.0011210787468133336	0.0	0.0	20.10587467684905	0.0
24	0.0011210787468133336	0.0	0.0	20.66574140300763	0.0
25	0.0011210787468133336	0.0	0.0	21.179868116296223	0.0
26	0.0011210787468133336	0.0	0.0	21.679869237374973	0.0
27	0.0011210787468133336	0.0	0.0	22.176507122213277	0.0
28	0.0011210787468133336	0.0	0.0	22.66664275032007	0.0
29	0.0011210787468133336	0.0	0.0	23.16059004616602	0.0
30	0.0011210787468133336	0.0	0.0	23.761712470207332	0.0
31	0.0011210787468133336	0.0	0.0	24.29893340568028	0.0
32	0.0011210787468133336	0.0	0.0	24.825616200933187	0.0
33	0.0011210787468133336	0.0	0.0	25.338397619725605	0.0
34	0.0013452944961760004	0.0	0.0	25.859475021244442	0.0
35	0.0013452944961760004	0.0	0.0	26.40319821344891	0.0
36	0.0013452944961760004	0.0	0.0	26.92449983071711	0.0
37	0.0015695102455386672	0.0	0.0	27.42651889354012	0.0
38	0.0015695102455386672	0.0	0.0	27.961497671519442	0.0
39	0.0015695102455386672	0.0	0.0	28.494682723503864	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	30	2.162502E-6	45.000004	2
TTGTGCG	35	1.2097007E-7	45.0	1
GTCCTCG	20	7.028638E-4	45.0	9
TAGCCGT	20	7.028638E-4	45.0	44
ATACGCG	20	7.028638E-4	45.0	1
GTAAGCG	25	3.8869526E-5	45.0	1
TAAGCGG	55	1.8189894E-12	45.0	2
CGATAGA	25	3.8869526E-5	45.0	10
GGCTACG	25	3.8869526E-5	45.0	1
CGTTTTT	2165	0.0	43.33718	1
CACAACG	125	0.0	43.2	12
ATGACGG	60	3.6379788E-12	41.250004	2
CGACAGG	55	6.002665E-11	40.909092	2
ACAACGA	135	0.0	40.000004	13
CCGATGA	225	0.0	40.0	18
GCGATAT	45	1.9237632E-8	40.0	9
AACGAGC	130	0.0	39.80769	15
CGTATGG	40	3.451878E-7	39.375	2
CGAATAT	80	0.0	39.375	14
GCTACGA	80	0.0	39.375	10
>>END_MODULE