##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935517.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	478663
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.185251419056833	31.0	31.0	33.0	28.0	34.0
2	31.262196994545224	31.0	31.0	34.0	28.0	34.0
3	31.03040761454301	31.0	31.0	34.0	28.0	34.0
4	34.78373720132954	35.0	35.0	37.0	32.0	37.0
5	34.823959654287044	35.0	35.0	37.0	33.0	37.0
6	35.02841038475921	36.0	35.0	37.0	32.0	37.0
7	35.37923340638403	37.0	35.0	37.0	33.0	37.0
8	35.18357174045205	37.0	35.0	37.0	32.0	37.0
9	37.1453903894807	39.0	37.0	39.0	34.0	39.0
10	36.551442246423896	38.0	35.0	39.0	32.0	39.0
11	36.75180868377125	39.0	37.0	39.0	32.0	39.0
12	36.82850147180793	39.0	35.0	39.0	33.0	39.0
13	36.88001788314534	39.0	37.0	39.0	33.0	39.0
14	38.00169012436725	40.0	37.0	41.0	33.0	41.0
15	38.140576982135656	40.0	37.0	41.0	33.0	41.0
16	38.110933997405276	40.0	37.0	41.0	33.0	41.0
17	37.95657905457493	40.0	37.0	41.0	33.0	41.0
18	37.869064456621885	39.0	37.0	41.0	33.0	41.0
19	37.62981471306535	39.0	37.0	40.0	33.0	41.0
20	37.53785648775861	39.0	35.0	41.0	33.0	41.0
21	37.51126993312623	39.0	35.0	41.0	33.0	41.0
22	37.6237499033767	39.0	35.0	41.0	33.0	41.0
23	37.64017690943315	39.0	35.0	41.0	33.0	41.0
24	37.63095121202182	39.0	35.0	41.0	33.0	41.0
25	37.2684790760932	39.0	35.0	41.0	33.0	41.0
26	37.292385248076414	39.0	35.0	41.0	33.0	41.0
27	37.33596287993849	39.0	35.0	41.0	33.0	41.0
28	37.209719573060795	39.0	35.0	41.0	33.0	41.0
29	37.12928929121323	39.0	35.0	41.0	32.0	41.0
30	36.81868454424094	39.0	35.0	41.0	31.0	41.0
31	36.694641114938904	39.0	35.0	41.0	31.0	41.0
32	36.46259268002749	39.0	35.0	41.0	30.0	41.0
33	36.257565343467114	39.0	35.0	41.0	30.0	41.0
34	35.9566856013521	39.0	35.0	41.0	28.0	41.0
35	35.89833348305593	39.0	35.0	41.0	27.0	41.0
36	35.7009816927567	39.0	35.0	41.0	26.0	41.0
37	35.61276096126085	39.0	35.0	41.0	25.0	41.0
38	35.45285722940775	39.0	35.0	41.0	25.0	41.0
39	35.3480945884683	39.0	35.0	41.0	24.0	41.0
40	35.21627951189041	38.0	35.0	40.0	23.0	41.0
41	35.07672412532408	38.0	35.0	40.0	23.0	41.0
42	35.066292986924	38.0	35.0	40.0	23.0	41.0
43	35.025951452274356	38.0	35.0	40.0	23.0	41.0
44	34.998782024096286	38.0	35.0	40.0	23.0	41.0
45	34.97559869887583	38.0	35.0	40.0	23.0	41.0
46	34.86609994923359	38.0	34.0	40.0	23.0	41.0
47	34.77032275316872	38.0	34.0	40.0	23.0	41.0
48	34.735045324163345	38.0	34.0	40.0	23.0	41.0
49	34.70064325005275	38.0	34.0	40.0	23.0	41.0
50	34.591858990563296	38.0	34.0	40.0	23.0	41.0
51	33.46545899724859	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	14.0
9	25.0
10	21.0
11	17.0
12	18.0
13	12.0
14	19.0
15	28.0
16	73.0
17	103.0
18	222.0
19	457.0
20	877.0
21	1381.0
22	2092.0
23	3005.0
24	4404.0
25	6341.0
26	8408.0
27	9024.0
28	8555.0
29	8538.0
30	9092.0
31	10807.0
32	13408.0
33	18442.0
34	32049.0
35	36805.0
36	37804.0
37	56280.0
38	98712.0
39	111600.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.557498699502574	18.86630050787297	20.54347212966116	18.032728662963297
2	37.32041122877682	24.414671700131407	22.516885575028777	15.748031496062993
3	29.270906671290657	24.239600721175442	31.651704852892326	14.837787754641575
4	25.16634876729557	23.91139486444534	33.42748447237409	17.494771895885
5	24.560285628928913	27.70947409764281	29.78838974393258	17.9418505294957
6	23.016610851475882	33.00129736369847	29.61018503623635	14.3719067485893
7	72.83475012691602	5.038826899091845	17.60340782554741	4.52301514844473
8	73.6860797680205	6.807921230594386	14.824417178683124	4.681581822701984
9	68.50707073661427	6.551791134890308	16.620879407850616	8.320258720644796
10	36.958778932150594	27.308983564637334	22.45212184773003	13.280115655482042
11	29.26693728155299	22.486175033374213	30.760890229660532	17.485997455412264
12	25.671505840225795	20.57522724756248	33.80708348044449	19.946183431767235
13	23.922467372660932	21.24459170648243	36.010930445846036	18.822010475010604
14	18.935033624909384	26.310786503239232	34.302003706156526	20.452176165694862
15	18.649446479046844	23.704986598086755	37.96491477302403	19.680652149842373
16	22.3787925952079	24.26091007660922	33.71077355049377	19.649523777689105
17	21.370776517090313	25.098242396007215	33.16425125819209	20.366729828710387
18	23.226988507572134	24.080407301170133	32.01500847151336	20.67759571974437
19	21.35970400887472	27.202436787468425	30.589370809943528	20.84848839371332
20	25.219204325381323	25.66544729799462	31.286312081777783	17.829036294846272
21	22.466328084685884	27.82270616279094	31.920578778806803	17.79038697371637
22	21.775654270332154	23.162015865024035	33.463626810511784	21.59870305413203
23	21.860682776817928	25.545112114368564	32.84168611319446	19.75251899561905
24	22.576426421093753	23.474135247554127	33.23026847698694	20.71916985436518
25	21.29389570532922	26.502361786893914	30.552601726057787	21.65114078171908
26	20.39159074338313	25.00611077104351	32.39126483559415	22.21103364997921
27	22.693836791228904	24.03507269206101	30.950376360821707	22.32071415588838
28	19.087332841686113	27.9175536859962	32.27030290622003	20.724810566097652
29	21.936936842830967	26.20277731932487	31.393485604694742	20.466800233149417
30	23.0471124778811	25.47993055657112	31.92475708379382	19.54819988175397
31	23.617868939107474	26.36364206132498	29.09332870934248	20.925160290225065
32	24.059933606733757	28.191441577895095	29.04402471049569	18.704600104875453
33	23.46222707834113	25.93536580015585	29.84417011550924	20.75823700599378
34	21.079966489994003	27.391296172881546	30.643897690024087	20.88483964710036
35	22.81667895784717	27.114692382741097	29.355308432028377	20.71332022738336
36	22.62510367419249	30.987145444707448	27.100068315286535	19.287682565813526
37	23.36549931789171	28.733994480459113	27.690880640450587	20.20962556119859
38	22.19223127753764	29.053216981467127	27.147700992138518	21.60685074885671
39	24.04739869177271	26.172275692919655	27.535029864434897	22.245295750872742
40	24.003735404658393	24.508683562339264	29.824532082070267	21.663048950932076
41	21.60622400310866	26.40939450093281	28.31532832076012	23.669053175198417
42	23.54913582207106	25.886688547057112	28.724593294238325	21.839582336633498
43	23.663412463465946	25.682996178940087	28.407042115225117	22.24654924236885
44	22.714101570415927	25.322826289059318	28.882115392248824	23.080956748275927
45	22.448361373241717	24.527068104282137	28.58002394168758	24.444546580788572
46	22.90045397283684	26.293237622293763	28.13440771482233	22.67190069004707
47	20.972375136578343	25.555140046337403	31.245155777655683	22.227329039428575
48	21.342990788926656	24.902070976866813	30.43163979668368	23.323298437522848
49	22.24404225937664	23.920796050666127	31.44529658653374	22.389865103423496
50	20.110599733006314	24.23145302645076	31.926637321037976	23.73130991950495
51	19.888313907697064	22.831511940551078	30.658730672727998	26.621443479023867
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	353.0
1	537.0
2	721.0
3	6701.0
4	12681.0
5	8329.5
6	3978.0
7	4002.5
8	4027.0
9	3786.5
10	3546.0
11	3528.5
12	3511.0
13	3320.0
14	3129.0
15	2989.0
16	2849.0
17	2708.5
18	2568.0
19	2482.0
20	2396.0
21	2406.0
22	2416.0
23	2304.5
24	2193.0
25	2508.5
26	3330.0
27	3836.0
28	4270.0
29	4704.0
30	5523.5
31	6343.0
32	7699.0
33	9055.0
34	10359.5
35	11664.0
36	12527.0
37	13390.0
38	14564.0
39	15738.0
40	17307.0
41	18876.0
42	20462.0
43	22048.0
44	24530.0
45	27012.0
46	36773.5
47	46535.0
48	43681.5
49	40828.0
50	41239.5
51	41651.0
52	37803.0
53	33955.0
54	31501.5
55	29048.0
56	26428.5
57	23809.0
58	22612.5
59	21416.0
60	20250.0
61	19084.0
62	17064.0
63	15044.0
64	12644.0
65	10244.0
66	8150.5
67	6057.0
68	5000.5
69	3944.0
70	3338.5
71	2733.0
72	2385.0
73	2037.0
74	1584.0
75	914.5
76	698.0
77	505.5
78	313.0
79	237.0
80	161.0
81	100.5
82	40.0
83	41.5
84	43.0
85	30.5
86	18.0
87	13.0
88	8.0
89	6.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	478663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.550694628058935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.0489094870439	30.352329310088784
2	9.300904962882605	7.729181237546659
3	4.051427493878754	5.050188798176347
4	2.686691937410836	4.465356650041027
5	2.0170158345172884	4.190420449999365
6	1.627882342899055	4.058378527212465
7	1.2402595245710368	3.6073551335494907
8	1.020517818846736	3.39225794027148
9	0.8200115459535662	3.066484440365923
>10	4.111164509165882	25.817626602774187
>50	0.04675498608373471	1.3332849314611512
>100	0.02032825481899676	1.5081281248794736
>500	0.004573857334274271	1.2587442946522742
>1k	0.003049238222849514	2.6577010052832617
>5k	5.082063704749191E-4	1.5125625536980774
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7163	1.4964599310997508	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC	4476	0.9351046560941623	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG	2176	0.4545995825873318	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC	2140	0.4470786336107031	No Hit
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	1410	0.29457050158462217	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCT	1292	0.26991850216122826	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTT	1092	0.22813545229106907	No Hit
CGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG	922	0.19261985990143377	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTT	841	0.17569772470401931	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC	735	0.15355270827283496	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCT	643	0.13433250533256175	No Hit
CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	599	0.12514023436112673	No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT	596	0.12451348861307433	Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	569	0.11887277688060285	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCT	532	0.1111429126546234	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCT	524	0.10947159065981703	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTC	484	0.1011149806857852	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.267457480523876E-4	0.0	0.0	0.1318255223403522	0.0
2	6.267457480523876E-4	0.0	0.0	0.636564764771875	0.0
3	6.267457480523876E-4	0.0	0.0	0.9900493666734216	0.0
4	6.267457480523876E-4	0.0	0.0	1.6245249789517886	0.0
5	6.267457480523876E-4	0.0	0.0	3.422031784366036	0.0
6	6.267457480523876E-4	0.0	0.0	4.590285858735687	0.0
7	6.267457480523876E-4	0.0	0.0	5.479638075222025	0.0
8	6.267457480523876E-4	0.0	0.0	6.6301343533968575	0.0
9	6.267457480523876E-4	0.0	0.0	7.239331220503778	0.0
10	6.267457480523876E-4	0.0	0.0	8.598116002281355	0.0
11	6.267457480523876E-4	0.0	0.0	9.584404894466461	0.0
12	6.267457480523876E-4	0.0	0.0	10.864219711989437	0.0
13	6.267457480523876E-4	0.0	0.0	11.35057441247809	0.0
14	6.267457480523876E-4	0.0	0.0	11.601063796449694	0.0
15	6.267457480523876E-4	0.0	0.0	11.939924330896686	0.0
16	6.267457480523876E-4	0.0	0.0	12.399120048969735	0.0
17	6.267457480523876E-4	0.0	0.0	12.921408172346725	0.0
18	6.267457480523876E-4	0.0	0.0	13.502819311289988	0.0
19	6.267457480523876E-4	0.0	0.0	13.995859299757868	0.0
20	6.267457480523876E-4	0.0	0.0	14.375458307828263	0.0
21	6.267457480523876E-4	0.0	0.0	14.830057890415596	0.0
22	6.267457480523876E-4	0.0	0.0	15.341691336075694	0.0
23	6.267457480523876E-4	0.0	0.0	15.837029392286432	0.0
24	6.267457480523876E-4	0.0	0.0	16.226865247575017	0.0
25	6.267457480523876E-4	0.0	0.0	16.588497544201243	0.0
26	6.267457480523876E-4	0.0	0.0	16.977706653741777	0.0
27	6.267457480523876E-4	0.0	0.0	17.345397492599176	0.0
28	0.0010445762467539794	0.0	0.0	17.717893382191647	0.0
29	0.0010445762467539794	0.0	0.0	18.082450492308784	0.0
30	0.0010445762467539794	0.0	0.0	18.53997488838703	0.0
31	0.0010445762467539794	0.0	0.0	18.922289794698983	0.0
32	0.0010445762467539794	0.0	0.0	19.33301717492265	0.0
33	0.0010445762467539794	0.0	0.0	19.740819741655404	0.0
34	0.0010445762467539794	0.0	0.0	20.137549800172565	0.0
35	0.0010445762467539794	0.0	0.0	20.53595118068453	0.0
36	0.0010445762467539794	0.0	0.0	20.941037849175725	0.0
37	0.0010445762467539794	0.0	0.0	21.337767907692886	0.0
38	0.0010445762467539794	0.0	0.0	21.836239692643886	0.0
39	0.0012534914961047751	0.0	0.0	22.325937037122152	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGA	25	3.8873823E-5	45.000004	41
AATAGCG	25	3.8873823E-5	45.000004	1
CCGTGTT	20	7.0291565E-4	45.0	30
CAACCGT	20	7.0291565E-4	45.0	27
CCGTCGA	20	7.0291565E-4	45.0	41
CGTTTTT	4005	0.0	43.876408	1
GCTACGA	75	0.0	42.0	10
CAACGAG	225	0.0	41.0	14
TCGACGG	50	1.0786607E-9	40.500004	2
AGCGACT	235	0.0	40.212765	19
ACAACGA	230	0.0	40.108692	13
GTCGAAT	45	1.9243089E-8	40.0	43
GGACCGA	175	0.0	39.857143	8
GACCGAT	170	0.0	39.705883	9
CGTTTCT	165	0.0	39.545452	1
TTTCGCA	40	3.4525874E-7	39.375	13
CGAATAT	80	0.0	39.375	14
TTCGCAA	40	3.4525874E-7	39.375	14
TCGAATG	40	3.4525874E-7	39.375	44
AACGAGC	235	0.0	39.255318	15
>>END_MODULE