##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935517.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478663 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.185251419056833 31.0 31.0 33.0 28.0 34.0 2 31.262196994545224 31.0 31.0 34.0 28.0 34.0 3 31.03040761454301 31.0 31.0 34.0 28.0 34.0 4 34.78373720132954 35.0 35.0 37.0 32.0 37.0 5 34.823959654287044 35.0 35.0 37.0 33.0 37.0 6 35.02841038475921 36.0 35.0 37.0 32.0 37.0 7 35.37923340638403 37.0 35.0 37.0 33.0 37.0 8 35.18357174045205 37.0 35.0 37.0 32.0 37.0 9 37.1453903894807 39.0 37.0 39.0 34.0 39.0 10 36.551442246423896 38.0 35.0 39.0 32.0 39.0 11 36.75180868377125 39.0 37.0 39.0 32.0 39.0 12 36.82850147180793 39.0 35.0 39.0 33.0 39.0 13 36.88001788314534 39.0 37.0 39.0 33.0 39.0 14 38.00169012436725 40.0 37.0 41.0 33.0 41.0 15 38.140576982135656 40.0 37.0 41.0 33.0 41.0 16 38.110933997405276 40.0 37.0 41.0 33.0 41.0 17 37.95657905457493 40.0 37.0 41.0 33.0 41.0 18 37.869064456621885 39.0 37.0 41.0 33.0 41.0 19 37.62981471306535 39.0 37.0 40.0 33.0 41.0 20 37.53785648775861 39.0 35.0 41.0 33.0 41.0 21 37.51126993312623 39.0 35.0 41.0 33.0 41.0 22 37.6237499033767 39.0 35.0 41.0 33.0 41.0 23 37.64017690943315 39.0 35.0 41.0 33.0 41.0 24 37.63095121202182 39.0 35.0 41.0 33.0 41.0 25 37.2684790760932 39.0 35.0 41.0 33.0 41.0 26 37.292385248076414 39.0 35.0 41.0 33.0 41.0 27 37.33596287993849 39.0 35.0 41.0 33.0 41.0 28 37.209719573060795 39.0 35.0 41.0 33.0 41.0 29 37.12928929121323 39.0 35.0 41.0 32.0 41.0 30 36.81868454424094 39.0 35.0 41.0 31.0 41.0 31 36.694641114938904 39.0 35.0 41.0 31.0 41.0 32 36.46259268002749 39.0 35.0 41.0 30.0 41.0 33 36.257565343467114 39.0 35.0 41.0 30.0 41.0 34 35.9566856013521 39.0 35.0 41.0 28.0 41.0 35 35.89833348305593 39.0 35.0 41.0 27.0 41.0 36 35.7009816927567 39.0 35.0 41.0 26.0 41.0 37 35.61276096126085 39.0 35.0 41.0 25.0 41.0 38 35.45285722940775 39.0 35.0 41.0 25.0 41.0 39 35.3480945884683 39.0 35.0 41.0 24.0 41.0 40 35.21627951189041 38.0 35.0 40.0 23.0 41.0 41 35.07672412532408 38.0 35.0 40.0 23.0 41.0 42 35.066292986924 38.0 35.0 40.0 23.0 41.0 43 35.025951452274356 38.0 35.0 40.0 23.0 41.0 44 34.998782024096286 38.0 35.0 40.0 23.0 41.0 45 34.97559869887583 38.0 35.0 40.0 23.0 41.0 46 34.86609994923359 38.0 34.0 40.0 23.0 41.0 47 34.77032275316872 38.0 34.0 40.0 23.0 41.0 48 34.735045324163345 38.0 34.0 40.0 23.0 41.0 49 34.70064325005275 38.0 34.0 40.0 23.0 41.0 50 34.591858990563296 38.0 34.0 40.0 23.0 41.0 51 33.46545899724859 36.0 32.0 40.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 14.0 9 25.0 10 21.0 11 17.0 12 18.0 13 12.0 14 19.0 15 28.0 16 73.0 17 103.0 18 222.0 19 457.0 20 877.0 21 1381.0 22 2092.0 23 3005.0 24 4404.0 25 6341.0 26 8408.0 27 9024.0 28 8555.0 29 8538.0 30 9092.0 31 10807.0 32 13408.0 33 18442.0 34 32049.0 35 36805.0 36 37804.0 37 56280.0 38 98712.0 39 111600.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.557498699502574 18.86630050787297 20.54347212966116 18.032728662963297 2 37.32041122877682 24.414671700131407 22.516885575028777 15.748031496062993 3 29.270906671290657 24.239600721175442 31.651704852892326 14.837787754641575 4 25.16634876729557 23.91139486444534 33.42748447237409 17.494771895885 5 24.560285628928913 27.70947409764281 29.78838974393258 17.9418505294957 6 23.016610851475882 33.00129736369847 29.61018503623635 14.3719067485893 7 72.83475012691602 5.038826899091845 17.60340782554741 4.52301514844473 8 73.6860797680205 6.807921230594386 14.824417178683124 4.681581822701984 9 68.50707073661427 6.551791134890308 16.620879407850616 8.320258720644796 10 36.958778932150594 27.308983564637334 22.45212184773003 13.280115655482042 11 29.26693728155299 22.486175033374213 30.760890229660532 17.485997455412264 12 25.671505840225795 20.57522724756248 33.80708348044449 19.946183431767235 13 23.922467372660932 21.24459170648243 36.010930445846036 18.822010475010604 14 18.935033624909384 26.310786503239232 34.302003706156526 20.452176165694862 15 18.649446479046844 23.704986598086755 37.96491477302403 19.680652149842373 16 22.3787925952079 24.26091007660922 33.71077355049377 19.649523777689105 17 21.370776517090313 25.098242396007215 33.16425125819209 20.366729828710387 18 23.226988507572134 24.080407301170133 32.01500847151336 20.67759571974437 19 21.35970400887472 27.202436787468425 30.589370809943528 20.84848839371332 20 25.219204325381323 25.66544729799462 31.286312081777783 17.829036294846272 21 22.466328084685884 27.82270616279094 31.920578778806803 17.79038697371637 22 21.775654270332154 23.162015865024035 33.463626810511784 21.59870305413203 23 21.860682776817928 25.545112114368564 32.84168611319446 19.75251899561905 24 22.576426421093753 23.474135247554127 33.23026847698694 20.71916985436518 25 21.29389570532922 26.502361786893914 30.552601726057787 21.65114078171908 26 20.39159074338313 25.00611077104351 32.39126483559415 22.21103364997921 27 22.693836791228904 24.03507269206101 30.950376360821707 22.32071415588838 28 19.087332841686113 27.9175536859962 32.27030290622003 20.724810566097652 29 21.936936842830967 26.20277731932487 31.393485604694742 20.466800233149417 30 23.0471124778811 25.47993055657112 31.92475708379382 19.54819988175397 31 23.617868939107474 26.36364206132498 29.09332870934248 20.925160290225065 32 24.059933606733757 28.191441577895095 29.04402471049569 18.704600104875453 33 23.46222707834113 25.93536580015585 29.84417011550924 20.75823700599378 34 21.079966489994003 27.391296172881546 30.643897690024087 20.88483964710036 35 22.81667895784717 27.114692382741097 29.355308432028377 20.71332022738336 36 22.62510367419249 30.987145444707448 27.100068315286535 19.287682565813526 37 23.36549931789171 28.733994480459113 27.690880640450587 20.20962556119859 38 22.19223127753764 29.053216981467127 27.147700992138518 21.60685074885671 39 24.04739869177271 26.172275692919655 27.535029864434897 22.245295750872742 40 24.003735404658393 24.508683562339264 29.824532082070267 21.663048950932076 41 21.60622400310866 26.40939450093281 28.31532832076012 23.669053175198417 42 23.54913582207106 25.886688547057112 28.724593294238325 21.839582336633498 43 23.663412463465946 25.682996178940087 28.407042115225117 22.24654924236885 44 22.714101570415927 25.322826289059318 28.882115392248824 23.080956748275927 45 22.448361373241717 24.527068104282137 28.58002394168758 24.444546580788572 46 22.90045397283684 26.293237622293763 28.13440771482233 22.67190069004707 47 20.972375136578343 25.555140046337403 31.245155777655683 22.227329039428575 48 21.342990788926656 24.902070976866813 30.43163979668368 23.323298437522848 49 22.24404225937664 23.920796050666127 31.44529658653374 22.389865103423496 50 20.110599733006314 24.23145302645076 31.926637321037976 23.73130991950495 51 19.888313907697064 22.831511940551078 30.658730672727998 26.621443479023867 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 353.0 1 537.0 2 721.0 3 6701.0 4 12681.0 5 8329.5 6 3978.0 7 4002.5 8 4027.0 9 3786.5 10 3546.0 11 3528.5 12 3511.0 13 3320.0 14 3129.0 15 2989.0 16 2849.0 17 2708.5 18 2568.0 19 2482.0 20 2396.0 21 2406.0 22 2416.0 23 2304.5 24 2193.0 25 2508.5 26 3330.0 27 3836.0 28 4270.0 29 4704.0 30 5523.5 31 6343.0 32 7699.0 33 9055.0 34 10359.5 35 11664.0 36 12527.0 37 13390.0 38 14564.0 39 15738.0 40 17307.0 41 18876.0 42 20462.0 43 22048.0 44 24530.0 45 27012.0 46 36773.5 47 46535.0 48 43681.5 49 40828.0 50 41239.5 51 41651.0 52 37803.0 53 33955.0 54 31501.5 55 29048.0 56 26428.5 57 23809.0 58 22612.5 59 21416.0 60 20250.0 61 19084.0 62 17064.0 63 15044.0 64 12644.0 65 10244.0 66 8150.5 67 6057.0 68 5000.5 69 3944.0 70 3338.5 71 2733.0 72 2385.0 73 2037.0 74 1584.0 75 914.5 76 698.0 77 505.5 78 313.0 79 237.0 80 161.0 81 100.5 82 40.0 83 41.5 84 43.0 85 30.5 86 18.0 87 13.0 88 8.0 89 6.0 90 4.0 91 3.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 478663.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.550694628058935 #Duplication Level Percentage of deduplicated Percentage of total 1 73.0489094870439 30.352329310088784 2 9.300904962882605 7.729181237546659 3 4.051427493878754 5.050188798176347 4 2.686691937410836 4.465356650041027 5 2.0170158345172884 4.190420449999365 6 1.627882342899055 4.058378527212465 7 1.2402595245710368 3.6073551335494907 8 1.020517818846736 3.39225794027148 9 0.8200115459535662 3.066484440365923 >10 4.111164509165882 25.817626602774187 >50 0.04675498608373471 1.3332849314611512 >100 0.02032825481899676 1.5081281248794736 >500 0.004573857334274271 1.2587442946522742 >1k 0.003049238222849514 2.6577010052832617 >5k 5.082063704749191E-4 1.5125625536980774 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7163 1.4964599310997508 No Hit GAATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC 4476 0.9351046560941623 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG 2176 0.4545995825873318 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC 2140 0.4470786336107031 No Hit GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC 1410 0.29457050158462217 No Hit GAACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCT 1292 0.26991850216122826 No Hit CGTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTT 1092 0.22813545229106907 No Hit CGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG 922 0.19261985990143377 No Hit GAATGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTT 841 0.17569772470401931 No Hit CGTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC 735 0.15355270827283496 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCT 643 0.13433250533256175 No Hit CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC 599 0.12514023436112673 No Hit CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT 596 0.12451348861307433 Illumina Single End Adapter 1 (95% over 21bp) GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 569 0.11887277688060285 No Hit GAATGACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCT 532 0.1111429126546234 No Hit CGTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCT 524 0.10947159065981703 No Hit GAATGATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTC 484 0.1011149806857852 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.267457480523876E-4 0.0 0.0 0.1318255223403522 0.0 2 6.267457480523876E-4 0.0 0.0 0.636564764771875 0.0 3 6.267457480523876E-4 0.0 0.0 0.9900493666734216 0.0 4 6.267457480523876E-4 0.0 0.0 1.6245249789517886 0.0 5 6.267457480523876E-4 0.0 0.0 3.422031784366036 0.0 6 6.267457480523876E-4 0.0 0.0 4.590285858735687 0.0 7 6.267457480523876E-4 0.0 0.0 5.479638075222025 0.0 8 6.267457480523876E-4 0.0 0.0 6.6301343533968575 0.0 9 6.267457480523876E-4 0.0 0.0 7.239331220503778 0.0 10 6.267457480523876E-4 0.0 0.0 8.598116002281355 0.0 11 6.267457480523876E-4 0.0 0.0 9.584404894466461 0.0 12 6.267457480523876E-4 0.0 0.0 10.864219711989437 0.0 13 6.267457480523876E-4 0.0 0.0 11.35057441247809 0.0 14 6.267457480523876E-4 0.0 0.0 11.601063796449694 0.0 15 6.267457480523876E-4 0.0 0.0 11.939924330896686 0.0 16 6.267457480523876E-4 0.0 0.0 12.399120048969735 0.0 17 6.267457480523876E-4 0.0 0.0 12.921408172346725 0.0 18 6.267457480523876E-4 0.0 0.0 13.502819311289988 0.0 19 6.267457480523876E-4 0.0 0.0 13.995859299757868 0.0 20 6.267457480523876E-4 0.0 0.0 14.375458307828263 0.0 21 6.267457480523876E-4 0.0 0.0 14.830057890415596 0.0 22 6.267457480523876E-4 0.0 0.0 15.341691336075694 0.0 23 6.267457480523876E-4 0.0 0.0 15.837029392286432 0.0 24 6.267457480523876E-4 0.0 0.0 16.226865247575017 0.0 25 6.267457480523876E-4 0.0 0.0 16.588497544201243 0.0 26 6.267457480523876E-4 0.0 0.0 16.977706653741777 0.0 27 6.267457480523876E-4 0.0 0.0 17.345397492599176 0.0 28 0.0010445762467539794 0.0 0.0 17.717893382191647 0.0 29 0.0010445762467539794 0.0 0.0 18.082450492308784 0.0 30 0.0010445762467539794 0.0 0.0 18.53997488838703 0.0 31 0.0010445762467539794 0.0 0.0 18.922289794698983 0.0 32 0.0010445762467539794 0.0 0.0 19.33301717492265 0.0 33 0.0010445762467539794 0.0 0.0 19.740819741655404 0.0 34 0.0010445762467539794 0.0 0.0 20.137549800172565 0.0 35 0.0010445762467539794 0.0 0.0 20.53595118068453 0.0 36 0.0010445762467539794 0.0 0.0 20.941037849175725 0.0 37 0.0010445762467539794 0.0 0.0 21.337767907692886 0.0 38 0.0010445762467539794 0.0 0.0 21.836239692643886 0.0 39 0.0012534914961047751 0.0 0.0 22.325937037122152 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTCGA 25 3.8873823E-5 45.000004 41 AATAGCG 25 3.8873823E-5 45.000004 1 CCGTGTT 20 7.0291565E-4 45.0 30 CAACCGT 20 7.0291565E-4 45.0 27 CCGTCGA 20 7.0291565E-4 45.0 41 CGTTTTT 4005 0.0 43.876408 1 GCTACGA 75 0.0 42.0 10 CAACGAG 225 0.0 41.0 14 TCGACGG 50 1.0786607E-9 40.500004 2 AGCGACT 235 0.0 40.212765 19 ACAACGA 230 0.0 40.108692 13 GTCGAAT 45 1.9243089E-8 40.0 43 GGACCGA 175 0.0 39.857143 8 GACCGAT 170 0.0 39.705883 9 CGTTTCT 165 0.0 39.545452 1 TTTCGCA 40 3.4525874E-7 39.375 13 CGAATAT 80 0.0 39.375 14 TTCGCAA 40 3.4525874E-7 39.375 14 TCGAATG 40 3.4525874E-7 39.375 44 AACGAGC 235 0.0 39.255318 15 >>END_MODULE