##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935516.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	528420
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206682184625866	31.0	31.0	33.0	28.0	34.0
2	31.223956322622158	31.0	31.0	34.0	28.0	34.0
3	31.010007191249386	31.0	31.0	34.0	27.0	34.0
4	34.80644941523788	35.0	35.0	37.0	32.0	37.0
5	34.8485768895954	35.0	35.0	37.0	33.0	37.0
6	35.03997956171227	37.0	35.0	37.0	32.0	37.0
7	35.51141705461565	37.0	35.0	37.0	33.0	37.0
8	35.322919268763485	37.0	35.0	37.0	33.0	37.0
9	37.23514817758601	39.0	37.0	39.0	34.0	39.0
10	36.52037205253397	38.0	35.0	39.0	32.0	39.0
11	36.716609893645206	39.0	37.0	39.0	32.0	39.0
12	36.765765868059496	39.0	35.0	39.0	33.0	39.0
13	36.794426781726656	39.0	37.0	39.0	33.0	39.0
14	37.69984103553991	40.0	37.0	41.0	33.0	41.0
15	37.940197191627874	40.0	37.0	41.0	33.0	41.0
16	37.927058022027936	40.0	37.0	41.0	33.0	41.0
17	37.87397903183074	40.0	37.0	41.0	33.0	41.0
18	37.83121002233072	39.0	37.0	41.0	33.0	41.0
19	37.68060822830324	39.0	36.0	41.0	33.0	41.0
20	37.756587562923436	39.0	36.0	41.0	33.0	41.0
21	37.67911509783884	39.0	36.0	41.0	33.0	41.0
22	37.80380946973998	39.0	36.0	41.0	33.0	41.0
23	37.8699651792135	40.0	36.0	41.0	34.0	41.0
24	37.8599844820408	40.0	36.0	41.0	34.0	41.0
25	37.42377464895348	39.0	35.0	41.0	33.0	41.0
26	37.56289504560766	39.0	35.0	41.0	33.0	41.0
27	37.63199159759282	39.0	35.0	41.0	33.0	41.0
28	37.49737330154044	39.0	35.0	41.0	33.0	41.0
29	37.436064115665566	39.0	35.0	41.0	33.0	41.0
30	37.151021914386284	39.0	35.0	41.0	32.0	41.0
31	37.086139813027515	39.0	35.0	41.0	32.0	41.0
32	37.049663146739334	39.0	35.0	41.0	32.0	41.0
33	37.0058551909466	39.0	35.0	41.0	32.0	41.0
34	36.859518943264824	39.0	35.0	41.0	31.0	41.0
35	36.831192990424285	39.0	35.0	41.0	31.0	41.0
36	36.66474395367322	39.0	35.0	41.0	31.0	41.0
37	36.636452064645546	39.0	35.0	41.0	31.0	41.0
38	36.442874985806746	39.0	35.0	41.0	30.0	41.0
39	36.38900117330911	39.0	35.0	41.0	30.0	41.0
40	36.30058665455509	39.0	35.0	41.0	30.0	41.0
41	36.09838007645433	39.0	35.0	41.0	30.0	41.0
42	36.111390560538965	39.0	35.0	41.0	30.0	41.0
43	36.0815279512509	39.0	35.0	40.0	30.0	41.0
44	36.06199424700049	39.0	35.0	40.0	30.0	41.0
45	36.01871049543924	38.0	35.0	40.0	30.0	41.0
46	35.91846069414481	38.0	35.0	40.0	29.0	41.0
47	35.89348056470232	38.0	35.0	40.0	29.0	41.0
48	35.80856894137239	38.0	35.0	40.0	29.0	41.0
49	35.76463608493244	38.0	35.0	40.0	29.0	41.0
50	35.648135952462056	38.0	35.0	40.0	29.0	41.0
51	34.46888270693766	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	20.0
9	24.0
10	32.0
11	25.0
12	18.0
13	15.0
14	29.0
15	32.0
16	69.0
17	94.0
18	186.0
19	365.0
20	597.0
21	971.0
22	1438.0
23	2025.0
24	2929.0
25	4200.0
26	5423.0
27	6351.0
28	6696.0
29	7539.0
30	8897.0
31	11317.0
32	15164.0
33	22535.0
34	38879.0
35	39035.0
36	44424.0
37	67677.0
38	113782.0
39	127610.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.27867983800764	20.41538927368381	23.745126982324667	13.560803905983876
2	35.312062374626244	22.97074296960751	24.477877445971007	17.23931720979524
3	32.479845577381624	22.343968812686878	28.565724234510427	16.610461375421067
4	27.78982627455433	25.537072783013514	27.00068127625752	19.672419666174633
5	24.455357480791797	29.605427500851594	26.805003595624694	19.134211422731916
6	24.84690208546232	33.432118390674084	26.305968736989517	15.415010786874078
7	78.5960031792892	5.594792021498051	11.092123689489421	4.717081109723327
8	79.77177245372998	5.375458915256804	9.967450134362817	4.885318496650392
9	74.1203966541766	6.784754551303887	12.640134741304266	6.454714053215245
10	40.079860716854014	27.444646304076304	18.25176942583551	14.22372355323417
11	29.28995874493774	24.65841565421445	26.983649369819464	19.06797623102835
12	26.466825631126756	22.33810226713599	31.253926800651	19.94114530108626
13	25.696415729911813	22.759168842965824	32.71412891260739	18.830286514514967
14	20.558835774573257	26.576776049354677	32.448809658983386	20.41557851708868
15	19.352219825139095	24.597100791037434	35.53101699405776	20.519662389765717
16	24.24567578819878	24.981454146323003	30.909314560387568	19.863555505090645
17	24.414102418530714	23.798493622497254	29.309072328829338	22.47833163014269
18	25.24128534120586	23.666780212709586	30.589303962756897	20.502630483327657
19	24.271791378070475	25.705688656750315	28.556262064267063	21.466257900912154
20	24.54089549979183	27.216418757806288	28.26047462245941	19.98221111994247
21	24.44873396162144	25.794254570228226	30.635290110139664	19.121721358010674
22	22.90886037621589	23.621172552136557	30.429204042239128	23.040763029408424
23	21.942962037772983	25.05885469891374	30.35066802921918	22.647515234094094
24	22.555732182733433	25.287082245183758	29.94076681427652	22.21641875780629
25	22.95711744445706	26.183906740850084	27.934218992468114	22.924756822224744
26	22.031906438060634	25.494871503728096	28.26293478672268	24.21028727148859
27	22.090004163354905	24.61432194088036	30.112789069300934	23.1828848264638
28	20.64740168805117	26.19109799023504	29.78369478823663	23.377805533477158
29	23.054388554558873	25.613148631770184	29.15124332916998	22.181219484500968
30	25.98009159380796	23.804170924643277	29.135157639756255	21.080579841792513
31	25.658377805533476	25.03198213542258	28.257257484576666	21.05238257446728
32	26.256386964914274	26.353847318420954	26.51413648234359	20.875629234321185
33	25.960788766511488	25.165966466068657	27.08129896673101	21.791945800688847
34	24.300934862420046	25.09197229476553	29.347299496612543	21.259793346201885
35	23.866810491654366	27.599636652662657	27.435562620642674	21.09799023504031
36	26.306157980394385	28.147685553158475	25.94091820900042	19.60523825744673
37	23.89576473259907	28.24173952537754	27.151886756746528	20.710608985276863
38	23.178721471556717	28.90219900836456	26.676885810529505	21.242193709549223
39	24.827977744975588	25.81885621286098	26.461905302600204	22.891260739563226
40	26.437682146777185	24.523863593353774	28.125354831384126	20.91309942848492
41	22.911509783884032	25.816017561787973	27.365353317436885	23.907119336891107
42	22.797963740963628	26.80065099731274	28.3231141894705	22.078271072253134
43	23.04833276560312	25.652132773172852	28.76045569811892	22.539078763105106
44	23.298323303432873	25.08269936792703	28.23246659853904	23.386510730101058
45	23.359638166609894	24.1018508004996	27.89031452253889	24.648196510351614
46	24.28068581809924	25.07948223004428	27.478331630142687	23.16150032171379
47	21.932175163695543	26.094962340562432	29.755308277506533	22.217554218235495
48	22.21566178418682	26.193747397903184	28.48170016274933	23.108890655160668
49	23.665644752280382	24.352030581734226	29.626622762196735	22.355701903788653
50	22.419098444419213	24.78199159759282	29.326482722077134	23.47242723591083
51	22.564058892547596	24.05643238333144	27.61912872336399	25.76038000075697
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	368.0
1	591.0
2	814.0
3	3409.0
4	6004.0
5	3989.5
6	1975.0
7	2103.0
8	2231.0
9	2027.5
10	1824.0
11	1817.5
12	1811.0
13	1787.5
14	1764.0
15	1716.0
16	1668.0
17	1593.0
18	1518.0
19	1560.5
20	1603.0
21	1659.5
22	1716.0
23	1827.0
24	1938.0
25	2458.0
26	3528.0
27	4078.0
28	4806.0
29	5534.0
30	6524.0
31	7514.0
32	8900.0
33	10286.0
34	11602.0
35	12918.0
36	14436.5
37	15955.0
38	17141.5
39	18328.0
40	19347.5
41	20367.0
42	22782.0
43	25197.0
44	28432.0
45	31667.0
46	37289.0
47	42911.0
48	46647.5
49	50384.0
50	50074.0
51	49764.0
52	44850.0
53	39936.0
54	36407.5
55	32879.0
56	30630.0
57	28381.0
58	27112.0
59	25843.0
60	23257.0
61	20671.0
62	18571.5
63	16472.0
64	14678.5
65	12885.0
66	11119.5
67	9354.0
68	7943.0
69	6532.0
70	5538.0
71	4544.0
72	3970.5
73	3397.0
74	2748.5
75	1656.0
76	1212.0
77	917.5
78	623.0
79	444.0
80	265.0
81	196.0
82	127.0
83	93.0
84	59.0
85	36.0
86	13.0
87	8.5
88	4.0
89	3.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	528420.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.92721854398159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.61033442787789	26.81297687969837
2	9.322294490740507	6.884928119818605
3	3.5851403054607247	3.971677787117553
4	2.1306268620255326	3.147124950787782
5	1.4896908674993328	2.750507011356069
6	1.187389311295005	2.6308190756987058
7	1.035115058144895	2.6756743979197917
8	0.9458953340231264	2.794342697536356
9	0.8139733476429416	2.705199452764848
>10	6.800366133681672	40.59058389009127
>50	0.04605538354999417	1.1484483430335262
>100	0.02742623964211263	1.7331824411495118
>500	0.004139809757300019	1.0368520314969405
>1k	0.0015524286589875072	1.117682921530705
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3706	0.7013360584383634	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	1080	0.2043828772567276	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	1063	0.2011657393739828	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	828	0.15669353923015783	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC	736	0.13928314598236252	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	711	0.134552060860679	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGC	683	0.12925324552439346	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTT	677	0.12811778509518942	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC	611	0.11562772037394496	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCG	600	0.11354604292040424	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	580	0.10976117482305742	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14268952726997464	0.0
2	0.0	0.0	0.0	0.6619734302259567	0.0
3	0.0	0.0	0.0	0.9920139283145982	0.0
4	0.0	0.0	0.0	1.3968055713258394	0.0
5	0.0	0.0	0.0	2.5277241588130654	0.0
6	0.0	0.0	0.0	3.415275727640892	0.0
7	0.0	0.0	0.0	4.221263388970894	0.0
8	0.0	0.0	0.0	5.389462927216987	0.0
9	0.0	0.0	0.0	5.931266795352182	0.0
10	0.0	0.0	0.0	6.982135422580523	0.0
11	0.0	0.0	0.0	8.237008440255858	0.0
12	0.0	0.0	0.0	9.331024563793951	0.0
13	0.0	0.0	0.0	9.805647023201242	0.0
14	0.0	0.0	0.0	10.047878581431437	0.0
15	0.0	0.0	0.0	10.363158093940426	0.0
16	1.8924340486734038E-4	0.0	0.0	10.94129669581015	0.0
17	1.8924340486734038E-4	0.0	0.0	11.612543052874607	0.0
18	1.8924340486734038E-4	0.0	0.0	12.389765716664774	0.0
19	1.8924340486734038E-4	0.0	0.0	12.859278604140645	0.0
20	3.7848680973468076E-4	0.0	0.0	13.332765603118732	0.0
21	3.7848680973468076E-4	0.0	0.0	13.889898187048182	0.0
22	3.7848680973468076E-4	0.0	0.0	14.485825668975437	0.0
23	5.677302146020212E-4	0.0	0.0	15.045039930358428	0.0
24	5.677302146020212E-4	0.0	0.0	15.477839597290034	0.0
25	5.677302146020212E-4	0.0	0.0	15.90363725824155	0.0
26	5.677302146020212E-4	0.0	0.0	16.300859165058096	0.0
27	5.677302146020212E-4	0.0	0.0	16.759963665266266	0.0
28	5.677302146020212E-4	0.0	0.0	17.171378827447864	0.0
29	5.677302146020212E-4	0.0	0.0	17.61061277014496	0.0
30	5.677302146020212E-4	0.0	0.0	18.199349002687256	0.0
31	5.677302146020212E-4	0.0	0.0	18.654100904583476	0.0
32	5.677302146020212E-4	0.0	0.0	19.115854812459787	0.0
33	5.677302146020212E-4	0.0	0.0	19.564929412209985	0.0
34	5.677302146020212E-4	0.0	0.0	20.044282956738957	0.0
35	5.677302146020212E-4	0.0	0.0	20.557321827334317	0.0
36	5.677302146020212E-4	0.0	0.0	20.99977290791416	0.0
37	5.677302146020212E-4	0.0	0.0	21.476098557965255	0.0
38	5.677302146020212E-4	0.0	0.0	21.994247000492035	0.0
39	5.677302146020212E-4	0.0	0.0	22.75216683698573	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCGC	20	7.0298224E-4	45.000004	20
TCGGACG	20	7.0298224E-4	45.000004	1
TAACCTA	25	3.8879356E-5	45.0	18
ATCCCCG	25	3.8879356E-5	45.0	38
GCGATAC	25	3.8879356E-5	45.0	9
CGTTTTT	1645	0.0	43.35866	1
TCACGAC	50	1.0786607E-9	40.5	25
ACGGGTC	45	1.9248546E-8	40.0	5
GACGGTC	45	1.9248546E-8	40.0	29
AGTACGG	45	1.9248546E-8	40.0	2
ACGGGAC	125	0.0	39.600002	5
CGACGGT	40	3.453497E-7	39.375004	28
TTGTACG	40	3.453497E-7	39.375004	1
ATAGCGG	75	0.0	39.000004	2
AATGCGG	110	0.0	38.863632	2
TAACGGG	145	0.0	38.793102	3
AACACGT	105	0.0	38.57143	41
CCATACG	35	6.241884E-6	38.571426	2
TTAACGG	35	6.241884E-6	38.571426	2
ACGGGAT	270	0.0	38.333332	5
>>END_MODULE