FastQCFastQC Report
Sat 14 Jan 2017
SRR2935515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346026
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25430.7349158733736771No Hit
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC13080.3780062769849664RNA PCR Primer, Index 47 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC10740.31038130082710547RNA PCR Primer, Index 17 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT9740.2814817383664811RNA PCR Primer, Index 17 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTG9680.27974776461884365RNA PCR Primer, Index 17 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC9590.2771468039973875RNA PCR Primer, Index 17 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTT6440.1861131822464208No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC5990.17310837913913985No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC5890.1702184228930774No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG5160.14912174229682162No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT4920.1421858473062718RNA PCR Primer, Index 17 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC4680.13524995231572196RNA PCR Primer, Index 47 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCT4240.12253414483304724No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCC4040.11675423234092236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGACG453.8380676E-1045.0000041
GCTAGCG207.0264435E-445.0000041
GTAAGCG207.0264435E-445.0000041
TACAGCG406.7921064E-945.0000041
ACGCCAC253.885136E-545.028
GCGCGAC253.885136E-545.09
ACACCGG253.885136E-545.018
CGACCCG253.885136E-545.032
CGTTATT253.885136E-545.01
CACACCG253.885136E-545.017
ACCCGCT253.885136E-545.034
CACCGGC253.885136E-545.019
AGGGCGC253.885136E-545.06
CGCCACA253.885136E-545.029
ACACGAC302.1610886E-644.99999626
GACACGA302.1610886E-644.99999625
CACGACC302.1610886E-644.99999627
GTATGCG302.1610886E-644.9999961
TACGAGG302.1610886E-644.9999962
TTTGACG302.1610886E-644.9999961
Produced by FastQC (version 0.11.5)