##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935515.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346026
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.155291798882164	31.0	31.0	33.0	28.0	34.0
2	31.18567390889702	31.0	31.0	34.0	28.0	34.0
3	30.929022674596705	31.0	30.0	34.0	27.0	34.0
4	34.705672406119774	35.0	35.0	37.0	32.0	37.0
5	34.80345118574905	35.0	35.0	37.0	33.0	37.0
6	35.04429146942715	36.0	35.0	37.0	32.0	37.0
7	35.48400698213429	37.0	35.0	37.0	33.0	37.0
8	35.39734008427112	37.0	35.0	37.0	33.0	37.0
9	37.32633096934912	39.0	37.0	39.0	34.0	39.0
10	36.509724702768	38.0	35.0	39.0	32.0	39.0
11	36.75407628328507	39.0	37.0	39.0	32.0	39.0
12	36.95954350251137	39.0	37.0	39.0	33.0	39.0
13	37.0882188043673	39.0	37.0	39.0	33.0	39.0
14	38.164981822175214	40.0	38.0	41.0	33.0	41.0
15	38.359204221648085	40.0	38.0	41.0	34.0	41.0
16	38.28101356545462	40.0	38.0	41.0	34.0	41.0
17	38.19545930074619	40.0	37.0	41.0	34.0	41.0
18	38.144107668209905	39.0	37.0	41.0	34.0	41.0
19	37.98694317768029	39.0	37.0	41.0	34.0	41.0
20	38.03950570188368	39.0	37.0	41.0	34.0	41.0
21	38.000687809586566	40.0	37.0	41.0	34.0	41.0
22	38.126022322022045	40.0	37.0	41.0	34.0	41.0
23	38.1443706542283	40.0	37.0	41.0	34.0	41.0
24	38.13555050776531	40.0	37.0	41.0	34.0	41.0
25	37.73362117297544	39.0	36.0	41.0	33.0	41.0
26	37.85749336755042	40.0	36.0	41.0	33.0	41.0
27	37.91500927675955	40.0	36.0	41.0	34.0	41.0
28	37.846156069197114	40.0	36.0	41.0	33.0	41.0
29	37.84162750775953	40.0	36.0	41.0	33.0	41.0
30	37.54341870264084	40.0	36.0	41.0	33.0	41.0
31	37.46533786478473	40.0	36.0	41.0	33.0	41.0
32	37.369087872009615	40.0	36.0	41.0	33.0	41.0
33	37.24405969493622	40.0	36.0	41.0	32.0	41.0
34	37.07291070613191	40.0	36.0	41.0	31.0	41.0
35	37.04425389999595	40.0	36.0	41.0	31.0	41.0
36	36.869793021333656	40.0	36.0	41.0	31.0	41.0
37	36.840199869373976	40.0	36.0	41.0	31.0	41.0
38	36.71509944339443	40.0	36.0	41.0	31.0	41.0
39	36.67099582112327	40.0	36.0	41.0	30.0	41.0
40	36.615176316230574	39.0	35.0	41.0	30.0	41.0
41	36.48885343875894	39.0	35.0	41.0	30.0	41.0
42	36.518660447480826	39.0	35.0	41.0	30.0	41.0
43	36.47909116655974	39.0	35.0	41.0	30.0	41.0
44	36.46489859143533	39.0	35.0	41.0	30.0	41.0
45	36.4507117962234	39.0	35.0	41.0	30.0	41.0
46	36.346485524209164	39.0	35.0	41.0	30.0	41.0
47	36.330131261812696	39.0	35.0	41.0	30.0	41.0
48	36.23704288117078	39.0	35.0	41.0	29.0	41.0
49	36.22727193910284	39.0	35.0	41.0	29.0	41.0
50	36.11905463751279	39.0	35.0	41.0	29.0	41.0
51	34.93516961153208	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	12.0
10	12.0
11	13.0
12	7.0
13	4.0
14	13.0
15	13.0
16	25.0
17	52.0
18	90.0
19	199.0
20	350.0
21	567.0
22	874.0
23	1245.0
24	2053.0
25	3234.0
26	4184.0
27	4415.0
28	4345.0
29	4441.0
30	5134.0
31	6162.0
32	8048.0
33	11444.0
34	18908.0
35	22920.0
36	26500.0
37	42338.0
38	80763.0
39	97643.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.27855132273297	21.37151543525631	25.246657765601427	14.103275476409289
2	35.32624716061799	22.564200378006277	25.63506788507222	16.474484576303514
3	30.09716032899262	22.990468924300487	30.71503297440077	16.197337772306128
4	26.47055423580887	24.946969302884757	30.511869050302575	18.0706074110038
5	23.85745579811922	29.246646205776443	28.51230832365198	18.38358967245236
6	23.321079918850028	33.37205874703057	28.523579153011624	14.78328218110778
7	77.32887124088941	5.12360342864409	12.80135018755816	4.746175142908336
8	78.76460150393324	4.1011369087872005	11.947946108095923	5.186315479183645
9	73.23987214833568	6.728107136457955	13.83595452364851	6.19606619155786
10	33.95582990873518	31.512371902689395	21.25620618103842	13.275592007537007
11	24.329096657476608	24.597284597111198	32.369243929646906	18.70437481576529
12	22.41305566633721	22.765341332732223	35.414968817372106	19.40663418355846
13	21.581904249969654	24.590926693369862	36.57846520203684	17.24870385462364
14	18.53502337974603	28.38919618756972	34.24424754209222	18.831532890592037
15	17.309681931415557	27.83692554894719	37.4607688439597	17.39262367567755
16	19.71210255876726	26.647708553692496	34.51127949922838	19.12890938831186
17	19.95052394906741	26.537023229468303	32.18139677365285	21.331056047811437
18	20.618970828781652	25.85412656852375	34.209568067139465	19.31733453555513
19	20.44586244964251	28.637154433481875	31.407755486581934	19.509227630293676
20	21.830440487131025	27.39562923017346	32.12330865310699	18.650621629588528
21	20.588337292573392	27.853109303925137	33.153289059203644	18.405264344297827
22	19.49911278343246	25.31630571113153	32.06868848005641	23.1158930253796
23	18.8020553368822	27.514984423135836	32.306820874731955	21.376139365250012
24	19.39362938045118	26.36507083282759	32.957639021345216	21.283660765376013
25	18.824885991226093	28.542941859860242	30.891898296659786	21.74027385225388
26	19.189887465103777	28.37965933195771	31.999329530150916	20.431123672787592
27	21.348684780912418	26.728916324206853	32.104812933132195	19.817585961748538
28	18.661603463323566	27.171079629854404	33.09346696491015	21.073849941911877
29	21.339725916549625	25.344627282342948	32.31982567783923	20.995821123268193
30	22.629224393542682	25.549814175813378	31.932860536491475	19.888100894152462
31	22.383000121378164	27.747625900943863	29.295197470710292	20.574176506967685
32	23.09566333165716	28.198170079704994	29.624074491512197	19.08209209712565
33	23.238138174588034	27.26240224722998	29.83330732372712	19.666152254454868
34	22.552351557397422	27.675376994792302	29.561362440972644	20.210909006837635
35	21.68536468357869	27.160097796119366	30.572269135845282	20.582268384456658
36	24.872986422985555	26.732384271702124	29.82579343748736	18.568835867824962
37	22.143711744204193	28.672700895308445	30.98697785715524	18.19660950333212
38	21.922341095755808	27.74300197095016	30.180969060128433	20.1536878731656
39	23.893002259945785	26.997971250715263	29.263118956379003	19.84590753295995
40	22.989023946177454	25.84198875229029	31.60427251131418	19.564714790218076
41	21.171241467404183	26.54193615508661	30.521694901539192	21.765127475970015
42	23.62308034656355	25.472074352794298	30.652031928236607	20.25281337240554
43	23.339864634449434	25.44722072907816	30.797396727413545	20.415517909058856
44	22.78325906145781	26.32952437100102	29.035679399813887	21.85153716772728
45	22.089380566778217	25.447509724702766	29.259940004508334	23.20316970401068
46	23.49476628923838	26.09457092819615	29.55355955910828	20.857103223457194
47	20.983394311410127	26.03041389953356	32.029385075109964	20.956806713946353
48	21.368914474634852	24.53341656407322	31.537225526405532	22.560443434886395
49	23.112136082259717	23.69330628334287	32.07389040129932	21.120667233098093
50	21.030500598220943	23.806881563813125	31.462953650881726	23.699664187084206
51	20.496147688323997	24.282279366290393	29.285949610722895	25.93562333466271
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	115.0
1	185.0
2	255.0
3	2290.0
4	4325.0
5	3040.5
6	1756.0
7	1861.5
8	1967.0
9	1851.0
10	1735.0
11	1725.5
12	1716.0
13	1682.0
14	1648.0
15	1603.0
16	1558.0
17	1516.5
18	1475.0
19	1417.5
20	1360.0
21	1430.5
22	1501.0
23	1660.5
24	1820.0
25	2292.0
26	3056.5
27	3349.0
28	4101.0
29	4853.0
30	5830.0
31	6807.0
32	7499.0
33	8191.0
34	9396.0
35	10601.0
36	11573.0
37	12545.0
38	13948.5
39	15352.0
40	16483.0
41	17614.0
42	19885.0
43	22156.0
44	24411.5
45	26667.0
46	31751.5
47	36836.0
48	38686.0
49	40536.0
50	38811.0
51	37086.0
52	30823.5
53	24561.0
54	20752.0
55	16943.0
56	14242.5
57	11542.0
58	10080.5
59	8619.0
60	7958.5
61	7298.0
62	6081.0
63	4864.0
64	3772.0
65	2680.0
66	2003.0
67	1326.0
68	1024.5
69	723.0
70	584.0
71	445.0
72	306.5
73	168.0
74	148.0
75	112.5
76	97.0
77	61.0
78	25.0
79	14.5
80	4.0
81	4.0
82	4.0
83	6.0
84	8.0
85	4.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	346026.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.63893541485187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.63357143642806	34.51835681981726
2	9.799109256994445	8.944418290060934
3	3.6583310526401016	5.00887003912766
4	2.1183182522228483	3.867111596052019
5	1.4219967669320999	3.2449209303071136
6	1.1606998583014287	3.1783863521427973
7	0.9215670163016806	2.9441536276215095
8	0.7316016787122742	2.6711617411317357
9	0.6670190946722964	2.739783724399971
>10	3.8257951121192617	27.114811184024624
>50	0.03642739233509964	1.16696353190499
>100	0.0191723117553156	1.633632277480592
>500	0.004473539409573639	1.5309651534539597
>1k	0.00191723117553156	1.4364647324749003
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2543	0.7349158733736771	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	1308	0.3780062769849664	RNA PCR Primer, Index 47 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	1074	0.31038130082710547	RNA PCR Primer, Index 17 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT	974	0.2814817383664811	RNA PCR Primer, Index 17 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTG	968	0.27974776461884365	RNA PCR Primer, Index 17 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC	959	0.2771468039973875	RNA PCR Primer, Index 17 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTT	644	0.1861131822464208	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC	599	0.17310837913913985	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC	589	0.1702184228930774	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG	516	0.14912174229682162	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT	492	0.1421858473062718	RNA PCR Primer, Index 17 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	468	0.13524995231572196	RNA PCR Primer, Index 47 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCT	424	0.12253414483304724	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCC	404	0.11675423234092236	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29188558085230587	0.0
2	0.0	0.0	0.0	1.2395022339361783	0.0
3	0.0	0.0	0.0	1.8556409055966894	0.0
4	0.0	0.0	0.0	2.617722367683353	0.0
5	0.0	0.0	0.0	4.481744146393623	0.0
6	0.0	0.0	0.0	5.769797645263651	0.0
7	0.0	0.0	0.0	6.902949489344731	0.0
8	0.0	0.0	0.0	8.709172143133753	0.0
9	0.0	0.0	0.0	9.440331073387549	0.0
10	0.0	0.0	0.0	10.94657626883529	0.0
11	0.0	0.0	0.0	12.779964511337297	0.0
12	0.0	0.0	0.0	14.352100709195263	0.0
13	0.0	0.0	0.0	15.028928462023085	0.0
14	0.0	0.0	0.0	15.319947056001572	0.0
15	0.0	0.0	0.0	15.74939455416645	0.0
16	0.0	0.0	0.0	16.599041690508805	0.0
17	0.0	0.0	0.0	17.75184523706311	0.0
18	0.0	0.0	0.0	18.92747943796131	0.0
19	0.0	0.0	0.0	19.634940726997392	0.0
20	0.0	0.0	0.0	20.324773282932497	0.0
21	0.0	0.0	0.0	21.162571598665995	0.0
22	0.0	0.0	0.0	22.10325235675932	0.0
23	0.0	0.0	0.0	23.04248813672961	0.0
24	0.0	0.0	0.0	23.738100605156838	0.0
25	0.0	0.0	0.0	24.324183731858298	0.0
26	0.0	0.0	0.0	24.87414240548398	0.0
27	0.0	0.0	0.0	25.47034037904666	0.0
28	0.0	0.0	0.0	26.075497216972135	0.0
29	0.0	0.0	0.0	26.714177547351934	0.0
30	0.0	0.0	0.0	27.470190101321865	0.0
31	0.0	0.0	0.0	28.131990081670164	0.0
32	2.8899562460624347E-4	0.0	0.0	28.755931635195044	0.0
33	2.8899562460624347E-4	0.0	0.0	29.33854681440123	0.0
34	2.8899562460624347E-4	0.0	0.0	29.933010814216274	0.0
35	2.8899562460624347E-4	0.0	0.0	30.52400686653604	0.0
36	2.8899562460624347E-4	0.0	0.0	31.145347459439463	0.0
37	5.779912492124869E-4	0.0	0.0	31.772467964835013	0.0
38	8.669868738187304E-4	0.0	0.0	32.365486986527024	0.0
39	8.669868738187304E-4	0.0	0.0	32.97497875882159	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGACG	45	3.8380676E-10	45.000004	1
GCTAGCG	20	7.0264435E-4	45.000004	1
GTAAGCG	20	7.0264435E-4	45.000004	1
TACAGCG	40	6.7921064E-9	45.000004	1
ACGCCAC	25	3.885136E-5	45.0	28
GCGCGAC	25	3.885136E-5	45.0	9
ACACCGG	25	3.885136E-5	45.0	18
CGACCCG	25	3.885136E-5	45.0	32
CGTTATT	25	3.885136E-5	45.0	1
CACACCG	25	3.885136E-5	45.0	17
ACCCGCT	25	3.885136E-5	45.0	34
CACCGGC	25	3.885136E-5	45.0	19
AGGGCGC	25	3.885136E-5	45.0	6
CGCCACA	25	3.885136E-5	45.0	29
ACACGAC	30	2.1610886E-6	44.999996	26
GACACGA	30	2.1610886E-6	44.999996	25
CACGACC	30	2.1610886E-6	44.999996	27
GTATGCG	30	2.1610886E-6	44.999996	1
TACGAGG	30	2.1610886E-6	44.999996	2
TTTGACG	30	2.1610886E-6	44.999996	1
>>END_MODULE