FastQCFastQC Report
Sat 14 Jan 2017
SRR2935514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415756
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC38630.9291507518833162No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC30830.7415407113787895No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG29310.7049808060497023No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27790.668420900720615No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC21420.5152060343085849No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC11980.28814978015951664No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG9490.2282588826138408No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT8970.2157515465802057No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT8590.2066115702479339No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTT7950.1912179258988445No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC7420.1784700641722549No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCT6650.15994958581475674No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCT5390.12964334850248704No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTCAAAT5310.12771914295885087No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTC5180.1245923089504421No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCC4840.11641443538998836No Hit
CGTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT4680.11256602430271602No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC4520.10871761321544367No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTA4260.1024639451986261No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAC253.886495E-545.00000434
GCGCCAG253.886495E-545.0000041
TAGCACG253.886495E-545.0000041
GCCGATT253.886495E-545.0000049
CACGTGA253.886495E-545.00000443
AACACGT253.886495E-545.00000441
TCGTTGA207.028087E-445.024
CGTTAGG453.8380676E-1045.02
CGAATAT453.8380676E-1045.014
CAATCGA207.028087E-445.041
AAGGGCG1500.043.55
CGTTTTT15900.043.3018881
TAACGGG556.002665E-1140.9090923
TACGAAT501.0786607E-940.50000412
GCGATGC501.0786607E-940.5000049
GCTACGA501.0786607E-940.50000410
CTAAGCG451.9232175E-840.01
ACTACGG451.9232175E-840.02
AAGACGC403.4511322E-739.37539
TGCGAAG403.4511322E-739.3751
Produced by FastQC (version 0.11.5)