##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935514.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415756
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.075332647033356	31.0	30.0	33.0	28.0	34.0
2	31.066000250146722	31.0	31.0	34.0	28.0	34.0
3	30.90484563061026	31.0	30.0	34.0	27.0	34.0
4	34.74887915027083	35.0	35.0	37.0	32.0	37.0
5	34.77504594040735	35.0	35.0	37.0	32.0	37.0
6	34.95557971502516	36.0	35.0	37.0	32.0	37.0
7	35.370573124621174	37.0	35.0	37.0	33.0	37.0
8	35.101773636459846	37.0	35.0	37.0	32.0	37.0
9	36.96013286639279	39.0	37.0	39.0	32.0	39.0
10	36.26375085386621	37.0	35.0	39.0	32.0	39.0
11	36.5731053791166	38.0	35.0	39.0	32.0	39.0
12	36.763676290901394	39.0	35.0	39.0	33.0	39.0
13	36.84223198222034	39.0	37.0	39.0	33.0	39.0
14	37.891005782237656	40.0	37.0	41.0	33.0	41.0
15	38.037786586363154	40.0	37.0	41.0	33.0	41.0
16	38.01290901394087	40.0	37.0	41.0	33.0	41.0
17	37.89116452919501	39.0	37.0	41.0	33.0	41.0
18	37.86558221649236	39.0	37.0	41.0	33.0	41.0
19	37.71468120724656	39.0	37.0	41.0	33.0	41.0
20	37.72027102435082	39.0	36.0	41.0	33.0	41.0
21	37.74186301580735	39.0	36.0	41.0	33.0	41.0
22	37.853683410461905	39.0	36.0	41.0	33.0	41.0
23	37.85445790319322	40.0	36.0	41.0	33.0	41.0
24	37.803117694032075	40.0	36.0	41.0	33.0	41.0
25	37.4578214144835	39.0	36.0	41.0	33.0	41.0
26	37.5162090264482	39.0	36.0	41.0	33.0	41.0
27	37.58552372064384	39.0	36.0	41.0	33.0	41.0
28	37.4936741742753	40.0	36.0	41.0	33.0	41.0
29	37.48149395318408	40.0	36.0	41.0	33.0	41.0
30	37.22151694744033	39.0	36.0	41.0	32.0	41.0
31	37.20199347694321	39.0	36.0	41.0	32.0	41.0
32	37.10844100866855	39.0	35.0	41.0	32.0	41.0
33	37.06506701045806	39.0	36.0	41.0	31.0	41.0
34	36.921033009746104	39.0	35.0	41.0	31.0	41.0
35	36.91958985558838	40.0	36.0	41.0	31.0	41.0
36	36.778947267147075	39.0	35.0	41.0	31.0	41.0
37	36.77933451351274	39.0	35.0	41.0	31.0	41.0
38	36.675102704470895	39.0	35.0	41.0	30.0	41.0
39	36.51527097624568	39.0	35.0	41.0	30.0	41.0
40	36.456616380761794	39.0	35.0	41.0	30.0	41.0
41	36.23642713514658	39.0	35.0	41.0	30.0	41.0
42	36.28805356988234	39.0	35.0	41.0	30.0	41.0
43	36.28429655855839	39.0	35.0	41.0	30.0	41.0
44	36.30470997411943	39.0	35.0	41.0	30.0	41.0
45	36.295714313202936	39.0	35.0	41.0	30.0	41.0
46	36.134785787817854	39.0	35.0	40.0	30.0	41.0
47	36.06441999634401	39.0	35.0	40.0	29.0	41.0
48	36.039371650679726	39.0	35.0	40.0	29.0	41.0
49	36.05126083568247	39.0	35.0	40.0	29.0	41.0
50	35.95980575145037	39.0	35.0	40.0	29.0	41.0
51	34.836137061160876	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	19.0
10	34.0
11	18.0
12	19.0
13	14.0
14	15.0
15	24.0
16	40.0
17	67.0
18	125.0
19	262.0
20	467.0
21	897.0
22	1227.0
23	1922.0
24	2660.0
25	3644.0
26	4429.0
27	4795.0
28	4994.0
29	5684.0
30	6883.0
31	8678.0
32	11368.0
33	15805.0
34	25804.0
35	31081.0
36	35526.0
37	53357.0
38	93728.0
39	102151.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.121205707193646	20.72609896189111	22.343393721317312	13.809301609597938
2	33.180038291690316	26.571113826378934	23.308623327143803	16.940224554786944
3	29.122369851547543	25.501255544117225	28.982143372555058	16.39423123178018
4	26.232213125006016	24.53554488690482	30.400282858214915	18.831959129874253
5	24.946362770471143	28.562185512656463	26.996603777215483	19.494847939656914
6	22.711879082923637	35.59419467187485	26.925408172100944	14.76851807310057
7	74.49609867326028	5.204494944149935	15.155042861678483	5.144363520911304
8	75.00288630831545	7.851720720807396	11.376384225362953	5.769008745514196
9	69.5614735566053	6.587517678638433	14.131606038156995	9.719402726599254
10	36.570247933884296	25.755010150184244	21.51550428616785	16.159237629763613
11	26.616813708040294	23.435861418716748	29.2876110026073	20.659713870635663
12	23.154205832267003	21.38369620642877	33.98002674645706	21.48207121484717
13	22.369851547542307	23.15588951211768	35.86526712783459	18.608991812505412
14	19.30627579638057	28.435668998162384	31.69118425230183	20.566870953155217
15	17.516043063720065	25.114730755539306	36.95725377384812	20.411972406892502
16	20.063931729187313	25.228018356920884	31.82034654941841	22.887703364473396
17	20.15196413280867	25.458201445078366	30.67736845649852	23.712465965614445
18	20.321775272034557	25.387967942735646	32.17704615206996	22.113210633159834
19	20.794167732997238	26.770509625838233	29.504084126266367	22.931238514898162
20	22.124034289342788	26.803221120080046	30.835393836769644	20.23735075380752
21	21.28724540355401	27.74415763091814	31.376576645917314	19.59202031961054
22	20.019915527376632	24.177161604402585	30.330049355872195	25.47287351234859
23	19.18240506450899	26.819576867200958	30.739424085280792	23.258593983009266
24	21.061151252176757	25.23932306448975	30.602324440296712	23.09720124303678
25	19.61102184935395	28.038080027708563	28.899883585564613	23.451014537372885
26	18.49834999374633	26.468649881180305	30.369255043823777	24.66374508124958
27	21.354352071888318	25.35790223111633	28.835903751238707	24.451841945756644
28	17.983143959437747	25.80431791723992	30.965518236658042	25.247019886664297
29	19.285350061093524	22.716930122475684	30.81759493549101	27.180124880939783
30	19.60741396395963	25.423565745292915	28.461886298694427	26.507133992053035
31	19.460933816950327	25.629696264154937	26.99443904597889	27.914930872915843
32	20.36506989676637	26.684401427760513	27.650593136358825	25.299935539114287
33	19.451793840618052	25.09957763688317	26.809715313789816	28.638913208708956
34	18.17869134780977	26.658905704307333	27.67753201396973	27.484870933913157
35	17.830650670104582	26.863352543318676	27.695811966634277	27.610184819942468
36	18.7545098567429	28.647812659348272	25.377865863631555	27.21981162027728
37	17.74766930603527	27.562560732737474	29.49951413810023	25.19025582312703
38	18.236898565504767	27.692925658318824	26.870568314107313	27.199607462069096
39	19.400802393711697	26.829919471997997	29.053338977669597	24.71593915662071
40	19.58239929189236	26.057831997613984	29.164461847814582	25.19530686267907
41	17.398907051251214	29.31743618853366	27.24410471526568	26.039552044949442
42	19.115057870481724	27.832430560232446	28.424364290593523	24.62814727869231
43	19.89243691011074	25.480570334523133	28.74185820529349	25.885134550072642
44	19.437602824733737	23.785345250579667	29.372997623606157	27.404054301080443
45	19.551371477501224	23.252821366378356	28.178306506701045	29.017500649419368
46	22.187773597975735	25.179672692637027	27.424739510674527	25.207814198712708
47	17.70172889868096	25.444250954887	31.22624808782074	25.627772058611303
48	18.494261056966106	25.61935365935789	28.758695003800305	27.127690279875694
49	19.18240506450899	23.285773386313128	32.11883893437497	25.412982614802914
50	18.882710051087656	22.86894236042294	30.912843109901	27.3355044785884
51	18.438699621893612	22.64453188889637	28.314444048913305	30.602324440296712
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	428.0
1	478.0
2	528.0
3	2617.0
4	4706.0
5	3191.0
6	1676.0
7	1861.0
8	2046.0
9	1823.0
10	1600.0
11	1622.5
12	1645.0
13	1529.5
14	1414.0
15	1422.0
16	1430.0
17	1373.5
18	1317.0
19	1387.0
20	1457.0
21	1372.0
22	1287.0
23	1386.5
24	1486.0
25	1825.0
26	2619.5
27	3075.0
28	3520.5
29	3966.0
30	4861.5
31	5757.0
32	6316.5
33	6876.0
34	8416.5
35	9957.0
36	11126.5
37	12296.0
38	13737.5
39	15179.0
40	16173.0
41	17167.0
42	19752.5
43	22338.0
44	24831.5
45	27325.0
46	38476.5
47	49628.0
48	48036.5
49	46445.0
50	45302.0
51	44159.0
52	37857.5
53	31556.0
54	26965.0
55	22374.0
56	20647.5
57	18921.0
58	16919.5
59	14918.0
60	13405.5
61	11893.0
62	10809.0
63	9725.0
64	8011.5
65	6298.0
66	5339.5
67	4381.0
68	3579.5
69	2778.0
70	2500.5
71	2223.0
72	1849.5
73	1476.0
74	1176.0
75	698.5
76	521.0
77	380.0
78	239.0
79	181.5
80	124.0
81	100.5
82	77.0
83	44.0
84	11.0
85	9.5
86	8.0
87	5.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	415756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.41165809418751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.61175596386828	30.89796527782669
2	9.963919986348174	8.25244895504984
3	3.708552433119809	4.607319161541742
4	2.106030393286011	3.488568423309102
5	1.467112655097788	3.037778382928262
6	1.1476268921427275	2.851507948626579
7	0.8791062951150532	2.5483674525226743
8	0.7774481706187031	2.5756334262090634
9	0.6376003672240064	2.3763679567388056
>10	4.611732888340313	30.26221540519103
>50	0.05393739371844666	1.55140650399475
>100	0.026382420840544565	2.0899072279165583
>500	0.005276484168108913	1.576899099130814
>1k	0.0035176561120726087	3.8836147790140876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	3863	0.9291507518833162	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC	3083	0.7415407113787895	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	2931	0.7049808060497023	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2779	0.668420900720615	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	2142	0.5152060343085849	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1198	0.28814978015951664	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	949	0.2282588826138408	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT	897	0.2157515465802057	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	859	0.2066115702479339	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTT	795	0.1912179258988445	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	742	0.1784700641722549	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCT	665	0.15994958581475674	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCT	539	0.12964334850248704	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTCAAAT	531	0.12771914295885087	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTC	518	0.1245923089504421	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCC	484	0.11641443538998836	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT	468	0.11256602430271602	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	452	0.10871761321544367	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTA	426	0.1024639451986261	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2147894438083876	0.0
2	0.0	0.0	0.0	1.2601141053887377	0.0
3	0.0	0.0	0.0	1.7803711792493675	0.0
4	0.0	0.0	0.0	2.603690626232694	0.0
5	0.0	0.0	0.0	5.048874820808359	0.0
6	0.0	0.0	0.0	6.32462309623914	0.0
7	0.0	0.0	0.0	7.520757367301975	0.0
8	0.0	0.0	0.0	9.215982451245441	0.0
9	0.0	0.0	0.0	9.793965691415156	0.0
10	0.0	0.0	0.0	11.670306622153378	0.0
11	0.0	0.0	0.0	13.399445828803433	0.0
12	2.405256929545214E-4	0.0	0.0	15.479752547167088	0.0
13	2.405256929545214E-4	0.0	0.0	16.092371487122254	0.0
14	2.405256929545214E-4	0.0	0.0	16.36440604585382	0.0
15	2.405256929545214E-4	0.0	0.0	16.84593848314877	0.0
16	2.405256929545214E-4	0.0	0.0	17.70774204100482	0.0
17	2.405256929545214E-4	0.0	0.0	18.78866450514244	0.0
18	2.405256929545214E-4	0.0	0.0	19.920578416186416	0.0
19	2.405256929545214E-4	0.0	0.0	20.719364242488382	0.0
20	2.405256929545214E-4	0.0	0.0	21.380809898113316	0.0
21	2.405256929545214E-4	0.0	0.0	22.139908985077785	0.0
22	2.405256929545214E-4	0.0	0.0	23.044285590586785	0.0
23	4.810513859090428E-4	0.0	0.0	23.91306439353852	0.0
24	4.810513859090428E-4	0.0	0.0	24.606499966326403	0.0
25	4.810513859090428E-4	0.0	0.0	25.212384186878843	0.0
26	4.810513859090428E-4	0.0	0.0	25.791329529820377	0.0
27	4.810513859090428E-4	0.0	0.0	26.354881228412818	0.0
28	4.810513859090428E-4	0.0	0.0	26.930699737345943	0.0
29	4.810513859090428E-4	0.0	0.0	27.52143083924225	0.0
30	4.810513859090428E-4	0.0	0.0	28.195624356593772	0.0
31	4.810513859090428E-4	0.0	0.0	28.793090177892804	0.0
32	4.810513859090428E-4	0.0	0.0	29.396328615822743	0.0
33	4.810513859090428E-4	0.0	0.0	29.99042707742041	0.0
34	4.810513859090428E-4	0.0	0.0	30.578031345308307	0.0
35	4.810513859090428E-4	0.0	0.0	31.14158304390075	0.0
36	4.810513859090428E-4	0.0	0.0	31.693830034924332	0.0
37	4.810513859090428E-4	0.0	0.0	32.25016596272814	0.0
38	4.810513859090428E-4	0.0	0.0	32.82285763765285	0.0
39	4.810513859090428E-4	0.0	0.0	33.38664986193825	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAC	25	3.886495E-5	45.000004	34
GCGCCAG	25	3.886495E-5	45.000004	1
TAGCACG	25	3.886495E-5	45.000004	1
GCCGATT	25	3.886495E-5	45.000004	9
CACGTGA	25	3.886495E-5	45.000004	43
AACACGT	25	3.886495E-5	45.000004	41
TCGTTGA	20	7.028087E-4	45.0	24
CGTTAGG	45	3.8380676E-10	45.0	2
CGAATAT	45	3.8380676E-10	45.0	14
CAATCGA	20	7.028087E-4	45.0	41
AAGGGCG	150	0.0	43.5	5
CGTTTTT	1590	0.0	43.301888	1
TAACGGG	55	6.002665E-11	40.909092	3
TACGAAT	50	1.0786607E-9	40.500004	12
GCGATGC	50	1.0786607E-9	40.500004	9
GCTACGA	50	1.0786607E-9	40.500004	10
CTAAGCG	45	1.9232175E-8	40.0	1
ACTACGG	45	1.9232175E-8	40.0	2
AAGACGC	40	3.4511322E-7	39.375	39
TGCGAAG	40	3.4511322E-7	39.375	1
>>END_MODULE