SRR2935513.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935513.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390606
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2161	0.5532429097351296	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	1610	0.41218004843755596	RNA PCR Primer, Index 40 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	1444	0.36968198133157193	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	1236	0.31643139122286906	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG	1114	0.2851978720244953	No Hit
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	870	0.22273083362774768	RNA PCR Primer, Index 40 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	783	0.20045775026497287	RNA PCR Primer, Index 40 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	556	0.14234292355980196	RNA PCR Primer, Index 40 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	472	0.12083787755436425	RNA PCR Primer, Index 40 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	467	0.11955781529213581	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGCG	20	7.027563E-4	45.0	1
CGGTCTA	20	7.027563E-4	45.0	31
TAAGCGA	20	7.027563E-4	45.0	22
TCGACAA	20	7.027563E-4	45.0	19
TACAACG	20	7.027563E-4	45.0	1
AAGGGCG	75	0.0	42.0	5
CGTTTTT	1110	0.0	41.959457	1
CGAAAGG	55	6.002665E-11	40.909092	2
CCGATGA	110	0.0	40.909092	18
TGAACGG	55	6.002665E-11	40.909092	2
GTATTAG	45	1.9226718E-8	40.0	1
GTTTGCG	40	3.4504046E-7	39.375	1
GGCCGAT	80	0.0	39.375	8
ATGCTCG	40	3.4504046E-7	39.375	13
TCGTAGG	40	3.4504046E-7	39.375	2
CGTAAGG	40	3.4504046E-7	39.375	2
TGGGCGA	160	0.0	39.375	6
CGATGCA	35	6.237704E-6	38.571426	10
CGTTTGG	35	6.237704E-6	38.571426	2
CTGCGGG	160	0.0	37.96875	3