##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935513.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390606
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.175435093162932	31.0	31.0	33.0	28.0	34.0
2	31.15634680470858	31.0	31.0	34.0	28.0	34.0
3	30.968920088273094	31.0	31.0	34.0	27.0	34.0
4	34.79376916893238	35.0	35.0	37.0	32.0	37.0
5	34.81898639549828	35.0	35.0	37.0	33.0	37.0
6	35.01407556463546	36.0	35.0	37.0	32.0	37.0
7	35.47070705519116	37.0	35.0	37.0	33.0	37.0
8	35.25565403501226	37.0	35.0	37.0	32.0	37.0
9	37.16806961490607	39.0	37.0	39.0	34.0	39.0
10	36.40866755759	38.0	35.0	39.0	32.0	39.0
11	36.699584747802135	39.0	35.0	39.0	32.0	39.0
12	36.85658182413992	39.0	37.0	39.0	33.0	39.0
13	36.92966313881507	39.0	37.0	39.0	33.0	39.0
14	37.97622156341684	40.0	37.0	41.0	33.0	41.0
15	38.17340235429051	40.0	37.0	41.0	33.0	41.0
16	38.12330583759594	40.0	37.0	41.0	34.0	41.0
17	38.039794575608155	40.0	37.0	41.0	33.0	41.0
18	38.015332585776974	39.0	37.0	41.0	34.0	41.0
19	37.88333256529597	39.0	37.0	41.0	34.0	41.0
20	37.94548726850074	39.0	37.0	41.0	34.0	41.0
21	37.88953830714326	39.0	36.0	41.0	33.0	41.0
22	38.02992529556638	40.0	37.0	41.0	34.0	41.0
23	38.05649170775666	40.0	37.0	41.0	34.0	41.0
24	38.04243150386835	40.0	37.0	41.0	34.0	41.0
25	37.62100684577298	39.0	36.0	41.0	33.0	41.0
26	37.75074115604983	40.0	36.0	41.0	33.0	41.0
27	37.81619073951757	40.0	36.0	41.0	33.0	41.0
28	37.72041648105764	40.0	36.0	41.0	33.0	41.0
29	37.68837396250954	40.0	36.0	41.0	33.0	41.0
30	37.42266119824068	40.0	36.0	41.0	33.0	41.0
31	37.406632258593056	39.0	36.0	41.0	33.0	41.0
32	37.39355770264665	40.0	36.0	41.0	33.0	41.0
33	37.34338438221635	40.0	36.0	41.0	33.0	41.0
34	37.248193832148	40.0	36.0	41.0	32.0	41.0
35	37.277502137703976	40.0	36.0	41.0	32.0	41.0
36	37.12595300635423	40.0	36.0	41.0	32.0	41.0
37	37.14810320373983	40.0	36.0	41.0	32.0	41.0
38	36.958597666190485	40.0	35.0	41.0	31.0	41.0
39	36.8605884190207	39.0	35.0	41.0	31.0	41.0
40	36.75771237512993	39.0	35.0	41.0	31.0	41.0
41	36.63298054817386	39.0	35.0	41.0	30.0	41.0
42	36.65842818594697	39.0	35.0	41.0	31.0	41.0
43	36.63256324787638	39.0	35.0	41.0	31.0	41.0
44	36.636444396655456	39.0	35.0	41.0	31.0	41.0
45	36.61579955249023	39.0	35.0	41.0	31.0	41.0
46	36.456224430756315	39.0	35.0	41.0	30.0	41.0
47	36.44547190775359	39.0	35.0	41.0	30.0	41.0
48	36.381366389661196	39.0	35.0	41.0	30.0	41.0
49	36.36050393491139	39.0	35.0	41.0	30.0	41.0
50	36.24020624363169	39.0	35.0	41.0	30.0	41.0
51	34.98207656820428	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	25.0
10	31.0
11	16.0
12	7.0
13	12.0
14	8.0
15	19.0
16	41.0
17	62.0
18	101.0
19	181.0
20	388.0
21	619.0
22	984.0
23	1378.0
24	2038.0
25	2847.0
26	3589.0
27	3998.0
28	4338.0
29	4841.0
30	5777.0
31	7480.0
32	10101.0
33	14177.0
34	24236.0
35	28456.0
36	31440.0
37	48965.0
38	88707.0
39	105716.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18445185173807	20.26543371069569	24.885690439983	12.664423997583244
2	32.687925940717754	25.31220718575751	25.058754857836284	16.941112015688443
3	31.587584420106197	24.15451887579812	28.640113055098997	15.617783648996685
4	27.746117571158663	26.130935008678826	27.41253334562193	18.710414074540584
5	25.232331300594463	29.726117878373604	26.23846023870601	18.803090582325925
6	23.865224804534492	35.80462153679155	25.65705595920186	14.673097699472102
7	77.94401519689917	5.8813740700347665	11.77836489966872	4.396245833397336
8	79.12013640343466	6.441529315985929	9.485005350660256	4.953328929919151
9	73.11357224415396	7.645043854933103	12.1385744202598	7.102809480653138
10	36.019159971941036	31.83002821257226	18.582919873222632	13.56789194226407
11	26.415108830893537	25.660128108631202	28.684147196919657	19.240615863555604
12	23.850888107197534	23.238762333399894	32.331812619365806	20.578536940036763
13	23.407986564466494	24.52445686958214	33.92139393660108	18.14616262935029
14	19.087264404540637	29.363860258162955	31.117033532511023	20.431841804785385
15	18.19787714474432	26.5062492639642	35.93313978791928	19.362733803372194
16	21.398800837672745	26.51879387413404	31.09143228726645	20.990973000926765
17	20.87679144713599	25.552858891056463	30.298817734494605	23.271531927312942
18	22.065713276293756	25.525465558644772	31.791882357157853	20.61693880790362
19	22.27461943748944	27.125799398882762	29.183115466736304	21.416465696891496
20	22.735185839439232	27.428918142578457	30.30086583411417	19.535030183868145
21	22.88341704940528	27.450679201036337	30.768344572279993	18.897559177278385
22	21.289483520478434	25.217994603257505	29.861036440812484	23.63148543545158
23	19.97434755226494	26.74485286964358	30.43527237164816	22.84552720644332
24	20.913913252740613	25.84676118646411	30.877149864569414	22.362175696225865
25	19.47947548168743	28.73586171231369	29.035908306580033	22.74875449941885
26	19.34097274491431	28.210012134990247	29.349267548373554	23.09974757172189
27	20.82917313098109	26.875419220390878	29.653154329426584	22.642253319201448
28	18.506884174846263	28.339298423475316	30.50081155947425	22.653005842204166
29	20.15304424407203	27.409205183740138	29.62985719625403	22.807893375933805
30	20.889336057305826	28.30064054315602	28.264798799813622	22.54522459972453
31	21.054464089133297	28.728693363645206	26.554635617476436	23.662206929745064
32	22.210104299473127	29.579678755574673	26.821144580472396	21.38907236447981
33	21.19091872628685	27.45835957460971	26.764053803577003	24.58666789552644
34	20.016845619370926	28.13141631208942	27.450935213488787	24.40080285505087
35	19.916744750464662	26.89026794263273	28.465256550078593	24.727730756824013
36	20.260825486551663	29.377172905690134	26.354433879663908	24.007567728094294
37	19.823300205321985	28.34288259780956	28.206427960656004	23.627389236212448
38	20.448482614194354	29.317522004270284	27.143720270553963	23.0902751109814
39	22.11896386640246	26.55591567973866	26.90153249054034	24.423587963318536
40	20.927993937625125	26.001392707741307	29.66339482752441	23.40721852710916
41	18.595464483392472	26.12274261020056	28.317025340112544	26.964767566294423
42	19.42008059272003	26.837785389881365	28.92249479014659	24.819639227252015
43	20.64868435200688	25.73821190662714	28.708980404806887	24.90412333655909
44	20.70577512890227	26.830105016307993	27.05232382503085	25.411796029758886
45	20.7664500801319	25.60329334418826	27.50776997793173	26.12248659774811
46	21.755426183929586	27.036963077884106	27.609150909100215	23.59845982908609
47	19.060639109486285	25.724131221742624	30.916063757341156	24.29916591142993
48	19.739840145824694	25.665504370132563	29.101703506858573	25.49295197718417
49	21.018366333338452	23.970701934942117	30.98851528138329	24.022416450336145
50	20.131283185614148	24.741811441708524	29.807785850703777	25.319119521973548
51	19.61644214374587	24.008335765451633	28.661874113556884	27.71334797724561
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	350.0
1	438.5
2	527.0
3	2108.0
4	3689.0
5	2508.5
6	1328.0
7	1424.5
8	1521.0
9	1348.0
10	1175.0
11	1195.0
12	1215.0
13	1190.5
14	1166.0
15	1152.5
16	1139.0
17	1084.0
18	1029.0
19	1095.0
20	1161.0
21	1210.5
22	1260.0
23	1565.5
24	1871.0
25	2215.5
26	2896.0
27	3232.0
28	3915.5
29	4599.0
30	5699.0
31	6799.0
32	7686.0
33	8573.0
34	9978.5
35	11384.0
36	12729.5
37	14075.0
38	15062.0
39	16049.0
40	17175.0
41	18301.0
42	21031.5
43	23762.0
44	26056.0
45	28350.0
46	34566.5
47	40783.0
48	41752.5
49	42722.0
50	40456.0
51	38190.0
52	33046.5
53	27903.0
54	24304.0
55	20705.0
56	18438.0
57	16171.0
58	15172.0
59	14173.0
60	12334.5
61	10496.0
62	9355.0
63	8214.0
64	6843.0
65	5472.0
66	4522.0
67	3572.0
68	3058.5
69	2545.0
70	2165.0
71	1785.0
72	1476.5
73	1168.0
74	996.5
75	621.0
76	417.0
77	307.5
78	198.0
79	126.5
80	55.0
81	49.0
82	43.0
83	41.0
84	39.0
85	23.0
86	7.0
87	5.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	390606.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.69784333884902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.53961460145374	29.193640996478443
2	9.628556249844292	7.6446583517122875
3	3.605003544761449	4.293325978678062
4	2.077056371161167	3.29818633713257
5	1.5334084523015097	3.0436504256966135
6	1.2955394166122545	3.085807247998628
7	0.989857119752464	2.7506635007444338
8	0.8887806439407814	2.822613981260999
9	0.7986679707613575	2.8534856384736735
>10	5.554831522009938	34.89258027565409
>50	0.05673174517570483	1.5322235898194838
>100	0.026735650025332163	2.059014433759786
>500	0.0019562670750243043	0.571833402586795
>1k	0.0032604451250405073	1.9583158400041216
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2161	0.5532429097351296	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	1610	0.41218004843755596	RNA PCR Primer, Index 40 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	1444	0.36968198133157193	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	1236	0.31643139122286906	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG	1114	0.2851978720244953	No Hit
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	870	0.22273083362774768	RNA PCR Primer, Index 40 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	783	0.20045775026497287	RNA PCR Primer, Index 40 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	556	0.14234292355980196	RNA PCR Primer, Index 40 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	472	0.12083787755436425	RNA PCR Primer, Index 40 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	467	0.11955781529213581	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5601245244568694E-4	0.0	0.0	0.2119783106250288	0.0
2	5.120249048913739E-4	0.0	0.0	1.020721647900954	0.0
3	5.120249048913739E-4	0.0	0.0	1.4375099204825323	0.0
4	5.120249048913739E-4	0.0	0.0	2.015330025652448	0.0
5	7.680373573370608E-4	0.0	0.0	3.6681464186418027	0.0
6	7.680373573370608E-4	0.0	0.0	4.676323456372918	0.0
7	7.680373573370608E-4	0.0	0.0	5.544973707521134	0.0
8	7.680373573370608E-4	0.0	0.0	6.967122880856925	0.0
9	7.680373573370608E-4	0.0	0.0	7.4714674121749285	0.0
10	7.680373573370608E-4	0.0	0.0	8.838829920687342	0.0
11	7.680373573370608E-4	0.0	0.0	10.395641644009565	0.0
12	7.680373573370608E-4	0.0	0.0	11.945029006210863	0.0
13	7.680373573370608E-4	0.0	0.0	12.54409814493377	0.0
14	7.680373573370608E-4	0.0	0.0	12.817519444145763	0.0
15	7.680373573370608E-4	0.0	0.0	13.233027654465113	0.0
16	7.680373573370608E-4	0.0	0.0	13.998248874825272	0.0
17	7.680373573370608E-4	0.0	0.0	15.004889837841713	0.0
18	7.680373573370608E-4	0.0	0.0	15.998474165783424	0.0
19	7.680373573370608E-4	0.0	0.0	16.697132148507702	0.0
20	7.680373573370608E-4	0.0	0.0	17.274184216320283	0.0
21	7.680373573370608E-4	0.0	0.0	17.928808057223904	0.0
22	7.680373573370608E-4	0.0	0.0	18.686092891558246	0.0
23	7.680373573370608E-4	0.0	0.0	19.453106199085525	0.0
24	0.0010240498097827478	0.0	0.0	20.02094181861006	0.0
25	0.0010240498097827478	0.0	0.0	20.536038872930778	0.0
26	0.0010240498097827478	0.0	0.0	21.047295740464815	0.0
27	0.0010240498097827478	0.0	0.0	21.551128246877926	0.0
28	0.0010240498097827478	0.0	0.0	22.048816454432345	0.0
29	0.0010240498097827478	0.0	0.0	22.597195127571005	0.0
30	0.0010240498097827478	0.0	0.0	23.221097474181143	0.0
31	0.0010240498097827478	0.0	0.0	23.76205178619888	0.0
32	0.0010240498097827478	0.0	0.0	24.30940640952776	0.0
33	0.0010240498097827478	0.0	0.0	24.852152808712617	0.0
34	0.0010240498097827478	0.0	0.0	25.377746373583612	0.0
35	0.0010240498097827478	0.0	0.0	25.930733270866295	0.0
36	0.0010240498097827478	0.0	0.0	26.450694561783486	0.0
37	0.0010240498097827478	0.0	0.0	26.9752640768447	0.0
38	0.0010240498097827478	0.0	0.0	27.483448794949386	0.0
39	0.0012800622622284347	0.0	0.0	28.057684725785062	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGCG	20	7.027563E-4	45.0	1
CGGTCTA	20	7.027563E-4	45.0	31
TAAGCGA	20	7.027563E-4	45.0	22
TCGACAA	20	7.027563E-4	45.0	19
TACAACG	20	7.027563E-4	45.0	1
AAGGGCG	75	0.0	42.0	5
CGTTTTT	1110	0.0	41.959457	1
CGAAAGG	55	6.002665E-11	40.909092	2
CCGATGA	110	0.0	40.909092	18
TGAACGG	55	6.002665E-11	40.909092	2
GTATTAG	45	1.9226718E-8	40.0	1
GTTTGCG	40	3.4504046E-7	39.375	1
GGCCGAT	80	0.0	39.375	8
ATGCTCG	40	3.4504046E-7	39.375	13
TCGTAGG	40	3.4504046E-7	39.375	2
CGTAAGG	40	3.4504046E-7	39.375	2
TGGGCGA	160	0.0	39.375	6
CGATGCA	35	6.237704E-6	38.571426	10
CGTTTGG	35	6.237704E-6	38.571426	2
CTGCGGG	160	0.0	37.96875	3
>>END_MODULE