SRR2935511.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935511.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285873
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	9140	3.1972239421001634	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	8556	2.9929374232613783	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	7945	2.779206150983129	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	2990	1.045918991999944	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT	1745	0.6104109167357533	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT	1727	0.604114414442777	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCGTCTAT	1678	0.586973935978564	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC	1355	0.4739867003879345	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTA	1300	0.4547473878260626	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1197	0.4187174024829207	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTT	804	0.2812437690862726	No Hit
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	553	0.19344254266754818	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT	550	0.1923931256187188	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATG	384	0.13432538225016005	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCGCCCATCGAATGC	366	0.1280288799571838	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGT	357	0.12488062881069566	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGCGTCTA	347	0.12138257198126441	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTAT	334	0.11683509810300378	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTG	294	0.10284287078527878	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	80	0.0	45.0	31
TTGTGCG	40	6.7848305E-9	45.0	1
GCGAACC	35	1.2076453E-7	45.0	33
GCTATCC	45	3.8198777E-10	45.0	33
TACGGAG	45	3.8198777E-10	45.0	29
ACGGCCT	35	1.2076453E-7	45.0	8
GGAGTAT	25	3.883429E-5	45.0	8
CCGACGT	25	3.883429E-5	45.0	44
CTCGAAT	20	7.024386E-4	45.0	43
CATCGAA	135	0.0	45.0	42
AGTAGTA	20	7.024386E-4	45.0	28
CAATAGG	20	7.024386E-4	45.0	2
TCGAATA	20	7.024386E-4	45.0	44
CCTGACG	20	7.024386E-4	45.0	42
ATCGAAT	200	0.0	45.0	43
CGCCCAT	130	0.0	44.999996	38
CGATGAA	1360	0.0	44.00735	19
TCGAATG	205	0.0	43.90244	44
GAGCGCC	190	0.0	43.81579	35
CGAATGC	185	0.0	43.783783	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content