##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935511.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285873
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0651443123345	31.0	30.0	33.0	28.0	34.0
2	31.01304425391695	31.0	30.0	34.0	28.0	34.0
3	30.898804713981384	31.0	30.0	34.0	28.0	34.0
4	34.81074463135728	35.0	35.0	37.0	32.0	37.0
5	34.73320670367611	35.0	35.0	37.0	32.0	37.0
6	34.871537360995966	35.0	35.0	37.0	32.0	37.0
7	35.264572030237204	36.0	35.0	37.0	33.0	37.0
8	34.76195023664354	37.0	35.0	37.0	32.0	37.0
9	36.43782029082845	39.0	35.0	39.0	32.0	39.0
10	36.095738317364706	37.0	35.0	39.0	32.0	39.0
11	36.39618292038772	38.0	35.0	39.0	32.0	39.0
12	36.5191361198854	38.0	35.0	39.0	32.0	39.0
13	36.37584171992458	39.0	35.0	39.0	32.0	39.0
14	37.48748570169271	39.0	36.0	41.0	32.0	41.0
15	37.503615241733215	39.0	36.0	41.0	32.0	41.0
16	37.78725867780448	39.0	36.0	41.0	33.0	41.0
17	37.367880142580795	39.0	36.0	41.0	32.0	41.0
18	37.48145505171877	39.0	36.0	40.0	32.0	41.0
19	37.39091834485943	39.0	36.0	40.0	32.0	41.0
20	36.92649882989999	39.0	35.0	40.0	30.0	41.0
21	37.37947969902719	39.0	35.0	40.0	32.0	41.0
22	37.40351134944538	39.0	35.0	41.0	32.0	41.0
23	37.20975747972002	39.0	35.0	41.0	31.0	41.0
24	37.27504521238452	39.0	35.0	41.0	31.0	41.0
25	37.25691828189441	39.0	35.0	41.0	32.0	41.0
26	37.00679322636275	39.0	35.0	41.0	31.0	41.0
27	36.91142570302197	39.0	35.0	41.0	31.0	41.0
28	36.81166462030342	39.0	35.0	41.0	30.0	41.0
29	36.792523253332774	39.0	35.0	41.0	30.0	41.0
30	36.59674400870316	39.0	35.0	41.0	30.0	41.0
31	36.75598604974937	39.0	35.0	41.0	30.0	41.0
32	36.44511723737464	39.0	35.0	41.0	30.0	41.0
33	36.27931633977325	39.0	35.0	41.0	29.0	41.0
34	36.11055608609418	39.0	35.0	41.0	29.0	41.0
35	36.110678518083205	39.0	35.0	41.0	29.0	41.0
36	36.100702060005666	39.0	35.0	41.0	28.0	41.0
37	35.83497217295792	39.0	35.0	41.0	27.0	41.0
38	35.89383397522676	39.0	35.0	41.0	26.0	41.0
39	35.594641676548676	39.0	35.0	41.0	26.0	41.0
40	35.44681729299374	39.0	35.0	40.0	25.0	41.0
41	35.56212374026229	39.0	35.0	40.0	25.0	41.0
42	35.45684971998055	39.0	35.0	40.0	25.0	41.0
43	35.415667096927656	39.0	35.0	40.0	25.0	41.0
44	35.58755461341225	39.0	35.0	40.0	26.0	41.0
45	35.62468298859983	39.0	35.0	40.0	26.0	41.0
46	35.47346548992035	39.0	35.0	40.0	26.0	41.0
47	35.08619212027718	38.0	34.0	40.0	24.0	41.0
48	35.22228052316938	38.0	35.0	40.0	25.0	41.0
49	35.27639196426385	38.0	35.0	40.0	25.0	41.0
50	35.092639039013825	38.0	35.0	40.0	24.0	41.0
51	33.842024255526056	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	13.0
10	4.0
11	8.0
12	9.0
13	6.0
14	5.0
15	10.0
16	21.0
17	55.0
18	118.0
19	274.0
20	745.0
21	1754.0
22	2831.0
23	3507.0
24	3523.0
25	3461.0
26	3479.0
27	3249.0
28	3321.0
29	3862.0
30	5002.0
31	6370.0
32	8800.0
33	11867.0
34	19458.0
35	21976.0
36	23297.0
37	33354.0
38	57952.0
39	67522.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.5942394000133	16.83579771436967	21.184232159035655	10.385730726581384
2	25.9597793425752	37.99729250401402	21.21501505913465	14.827913094276129
3	25.383299577084927	36.47004089228433	24.66479870431975	13.481860826310985
4	23.269074029376682	21.15239984188783	40.16923598940789	15.409290139327602
5	34.36141223550318	24.303449433839504	22.92731387714125	18.40782445351607
6	20.42935149524439	43.186659810475284	23.14454320624893	13.239445488031398
7	67.55167504451278	4.257135161417833	23.87878533474655	4.312404459322846
8	66.19932627425466	17.29614199312282	10.82193841321146	5.682593319411067
9	61.88167472968766	5.903320705348179	11.560728015587342	20.65427654937682
10	40.28816992160855	27.664032629874104	18.117835542356218	13.929961906161129
11	31.730523694087935	20.541289313786194	28.791106540316854	18.93708045180902
12	23.36527059218604	18.764976055801004	31.54512668212808	26.324626669884875
13	25.997208550650114	20.886197717168113	38.00114036652639	15.115453365655378
14	19.513210411616345	33.28890801159955	27.09035130984738	20.107530266936717
15	15.04724125748147	22.98468200914392	39.49970791225474	22.46836882111987
16	18.355703406757545	24.6546543395144	27.326470145833987	29.663172107894063
17	18.103843315038496	28.426259213007178	31.532883483225067	21.937013988729262
18	18.235720057508054	24.62317182804952	30.474721292322116	26.666386822120312
19	18.62120592011138	25.92864663679326	27.519912688501535	27.930234754593826
20	24.439523844504375	26.07836346909292	33.08007401888251	16.40203866752019
21	20.319162705117307	33.23538774210925	28.86491553941785	17.58053401335558
22	18.169606783431806	22.094426546053665	34.32748108425769	25.408485586256834
23	22.044054527709857	28.110734486992477	29.773710703704094	20.071500281593575
24	22.131855754128583	27.206486796584496	26.297341826615316	24.364315622671604
25	17.314331888635863	36.269602235957926	25.36965715545015	21.046408719956062
26	17.095003725430523	25.41338286581804	32.6760484550832	24.815564953668236
27	26.735298541660107	25.90485985035313	25.652649952951133	21.707191655035626
28	15.873832086276074	29.55158409503521	31.860301602459835	22.714282216228884
29	22.10841877337139	24.61442668597594	30.743372056822437	22.533782483830233
30	22.206014558912525	30.032916714764944	28.90164513612688	18.859423590195647
31	22.692594263886413	22.29031772850182	23.112011277735217	31.90507672987655
32	30.51809719700706	26.38549285871698	24.85404357879198	18.24236636548397
33	24.094265635439513	22.34908508323626	27.168008171460755	26.38864110986347
34	21.13281072364302	25.201400621954505	25.925498385646772	27.740290268755707
35	25.23078429932173	22.426392139166694	29.259146544094754	23.083677017416825
36	18.947224816614373	33.69118454698415	24.784782053569245	22.576808582832236
37	25.393443941890283	23.163082907444917	29.833877281170313	21.6095958694945
38	19.18544248669864	24.610928629146507	26.432366820231362	29.771262063923494
39	25.28150612334848	24.631567164440156	25.849240746765172	24.237685965446197
40	19.221122666358838	21.783799099600174	34.72590975712991	24.269168476911076
41	17.82749682551343	27.460445722401204	24.512283426556547	30.199774025528818
42	19.789556901141417	23.232344432667652	33.495293364535996	23.48280530165493
43	22.558618687319193	27.960667849009873	24.984171292846824	24.496542170824107
44	22.443532617630908	22.263032885232253	29.495265380081364	25.798169117055476
45	19.66362685528189	20.49966243751596	28.385331948102827	31.45137875909932
46	28.345803905930257	25.98741399152771	25.09645891707157	20.57032318547047
47	17.457752218642543	24.21599801310372	36.8940053800114	21.43224438824233
48	20.857513651166776	25.425975870403988	26.580684429799245	27.135826048629987
49	20.474126622661114	19.793754569336734	36.6960153634656	23.03610344453656
50	22.13745264505567	22.011522599196145	30.525443116348868	25.325581639399314
51	19.803898934142083	21.301766868504547	27.896653409031284	30.997680788322086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	266.0
1	298.0
2	330.0
3	1319.0
4	2308.0
5	1583.0
6	858.0
7	918.0
8	978.0
9	836.0
10	694.0
11	737.0
12	780.0
13	755.5
14	731.0
15	708.0
16	685.0
17	659.5
18	634.0
19	627.5
20	621.0
21	735.0
22	849.0
23	973.0
24	1097.0
25	1355.0
26	1923.0
27	2233.0
28	2732.5
29	3232.0
30	3703.5
31	4175.0
32	4555.0
33	4935.0
34	5705.5
35	6476.0
36	6903.5
37	7331.0
38	8395.0
39	9459.0
40	10598.0
41	11737.0
42	12930.5
43	14124.0
44	15996.0
45	17868.0
46	36015.5
47	54163.0
48	41302.0
49	28441.0
50	27872.0
51	27303.0
52	23116.5
53	18930.0
54	17345.0
55	15760.0
56	14142.5
57	12525.0
58	11152.0
59	9779.0
60	8914.0
61	8049.0
62	7067.0
63	6085.0
64	4990.5
65	3896.0
66	3162.0
67	2428.0
68	2081.5
69	1735.0
70	1349.5
71	964.0
72	885.0
73	806.0
74	637.0
75	366.5
76	265.0
77	199.5
78	134.0
79	105.5
80	77.0
81	52.0
82	27.0
83	20.0
84	13.0
85	11.0
86	9.0
87	5.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	285873.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.06595570207903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.91677788302154	25.93747325365311
2	9.841501505921995	7.098863147090534
3	4.074735112133779	4.408776481557687
4	2.548829072984873	3.677038257537743
5	1.8862518163853579	3.4014737226360205
6	1.4896087568827245	3.2234498063496804
7	1.2618206407957229	3.1856137134435256
8	1.077992385375805	3.110306049451383
9	0.965097903430376	3.1326460409960353
>10	4.848468035542986	25.518778211624525
>50	0.05095237375019585	1.2654535183767188
>100	0.024976653799115612	1.7929660328708863
>500	0.0029971984558938734	0.6871354953144655
>1k	0.0069934630637523715	4.320990487577908
>5k	0.0029971984558938734	9.239035781519775
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	9140	3.1972239421001634	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	8556	2.9929374232613783	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	7945	2.779206150983129	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	2990	1.045918991999944	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT	1745	0.6104109167357533	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT	1727	0.604114414442777	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCGTCTAT	1678	0.586973935978564	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC	1355	0.4739867003879345	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTA	1300	0.4547473878260626	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1197	0.4187174024829207	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTT	804	0.2812437690862726	No Hit
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	553	0.19344254266754818	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT	550	0.1923931256187188	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATG	384	0.13432538225016005	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCGCCCATCGAATGC	366	0.1280288799571838	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGT	357	0.12488062881069566	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGCGTCTA	347	0.12138257198126441	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTAT	334	0.11683509810300378	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTG	294	0.10284287078527878	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.498056829431251E-4	0.0	0.0	0.20043865632641067	0.0
2	3.498056829431251E-4	0.0	0.0	1.6570295201015837	0.0
3	3.498056829431251E-4	0.0	0.0	1.9945920041416993	0.0
4	3.498056829431251E-4	0.0	0.0	2.991888006212549	0.0
5	3.498056829431251E-4	0.0	0.0	7.199000954969515	0.0
6	3.498056829431251E-4	0.0	0.0	8.08400933281562	0.0
7	3.498056829431251E-4	0.0	0.0	9.300283692408867	0.0
8	3.498056829431251E-4	0.0	0.0	10.737985049305111	0.0
9	3.498056829431251E-4	0.0	0.0	11.06820161400342	0.0
10	3.498056829431251E-4	0.0	0.0	14.706880327977808	0.0
11	3.498056829431251E-4	0.0	0.0	15.927702161449314	0.0
12	3.498056829431251E-4	0.0	0.0	20.008885064346757	0.0
13	3.498056829431251E-4	0.0	0.0	20.58501502415408	0.0
14	3.498056829431251E-4	0.0	0.0	20.876053352362764	0.0
15	3.498056829431251E-4	0.0	0.0	21.643527020739977	0.0
16	3.498056829431251E-4	0.0	0.0	22.357130613943955	0.0
17	3.498056829431251E-4	0.0	0.0	23.206808617812804	0.0
18	3.498056829431251E-4	0.0	0.0	24.034099057973297	0.0
19	3.498056829431251E-4	0.0	0.0	25.23253332773644	0.0
20	3.498056829431251E-4	0.0	0.0	25.848541135399287	0.0
21	3.498056829431251E-4	0.0	0.0	26.411728284937716	0.0
22	6.996113658862502E-4	0.0	0.0	27.12603148950758	0.0
23	6.996113658862502E-4	0.0	0.0	27.73504318351156	0.0
24	6.996113658862502E-4	0.0	0.0	28.233166476022568	0.0
25	6.996113658862502E-4	0.0	0.0	28.65887999216435	0.0
26	6.996113658862502E-4	0.0	0.0	29.07514875486667	0.0
27	6.996113658862502E-4	0.0	0.0	29.55298331776698	0.0
28	0.0010494170488293752	0.0	0.0	29.957358687249233	0.0
29	0.0010494170488293752	0.0	0.0	30.407558601197035	0.0
30	0.0010494170488293752	0.0	0.0	30.92771965173346	0.0
31	0.0010494170488293752	0.0	0.0	31.402405963487283	0.0
32	0.0010494170488293752	0.0	0.0	31.840362678532074	0.0
33	0.0010494170488293752	0.0	0.0	32.24578746506316	0.0
34	0.0010494170488293752	0.0	0.0	32.662056227765476	0.0
35	0.0010494170488293752	0.0	0.0	33.094765857566124	0.0
36	0.0010494170488293752	0.0	0.0	33.51383306573199	0.0
37	0.0010494170488293752	0.0	0.0	33.93429949662962	0.0
38	0.0010494170488293752	0.0	0.0	34.38205077079682	0.0
39	0.0010494170488293752	0.0	0.0	34.81546001196335	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	80	0.0	45.0	31
TTGTGCG	40	6.7848305E-9	45.0	1
GCGAACC	35	1.2076453E-7	45.0	33
GCTATCC	45	3.8198777E-10	45.0	33
TACGGAG	45	3.8198777E-10	45.0	29
ACGGCCT	35	1.2076453E-7	45.0	8
GGAGTAT	25	3.883429E-5	45.0	8
CCGACGT	25	3.883429E-5	45.0	44
CTCGAAT	20	7.024386E-4	45.0	43
CATCGAA	135	0.0	45.0	42
AGTAGTA	20	7.024386E-4	45.0	28
CAATAGG	20	7.024386E-4	45.0	2
TCGAATA	20	7.024386E-4	45.0	44
CCTGACG	20	7.024386E-4	45.0	42
ATCGAAT	200	0.0	45.0	43
CGCCCAT	130	0.0	44.999996	38
CGATGAA	1360	0.0	44.00735	19
TCGAATG	205	0.0	43.90244	44
GAGCGCC	190	0.0	43.81579	35
CGAATGC	185	0.0	43.783783	45
>>END_MODULE