SRR2935509.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935509.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453763
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3433	0.7565623464231328	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	1133	0.24968981604934737	TruSeq Adapter, Index 21 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTG	1006	0.2217016371982731	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTC	999	0.22015898167104855	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	925	0.2038509089546746	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTT	867	0.1910689060148139	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGC	824	0.18159259349043444	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCG	814	0.17938879988011364	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTC	779	0.1716755222439908	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT	564	0.12429395962209348	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT	525	0.11569916454184234	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCT	518	0.11415650901461777	No Hit

Adapter graph

$Kmer graph$

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATG	20	7.028768E-4	45.000004	1
CTCACGA	35	1.2097553E-7	45.0	24
CCATACG	50	2.1827873E-11	45.0	2
CAACGAG	85	0.0	42.35294	14
CGTTTTT	1870	0.0	41.631016	1
AACACGT	60	3.6379788E-12	41.249996	41
TCACGAC	55	6.002665E-11	40.909092	25
CATACGG	55	6.002665E-11	40.909092	3
ACAACGA	90	0.0	40.0	13
TATTGCG	40	3.45206E-7	39.375004	1
AATTATC	80	0.0	39.375004	45
CGGTCTA	40	3.45206E-7	39.375004	31
ACGGTCT	40	3.45206E-7	39.375004	30
ATAGCGG	70	0.0	38.571426	2
TACGGGT	70	0.0	38.571426	4
TACGGGA	135	0.0	38.333332	4
CGCACGG	65	9.094947E-12	38.076927	2
ATTGCGG	95	0.0	37.894737	2
AACGTAG	30	1.1388333E-4	37.499996	1
TCCGAAC	60	1.546141E-10	37.499996	17