##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935509.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453763
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.200703891679137	31.0	31.0	33.0	28.0	34.0
2	31.21584174998843	31.0	31.0	34.0	28.0	34.0
3	30.951514336779333	31.0	30.0	34.0	27.0	34.0
4	34.792488589858586	35.0	35.0	37.0	32.0	37.0
5	34.83593417709245	35.0	35.0	37.0	33.0	37.0
6	35.03523425224181	36.0	35.0	37.0	32.0	37.0
7	35.4780072416658	37.0	35.0	37.0	33.0	37.0
8	35.3150411117698	37.0	35.0	37.0	33.0	37.0
9	37.20802489405262	39.0	37.0	39.0	34.0	39.0
10	36.485976159360725	38.0	35.0	39.0	32.0	39.0
11	36.70937251384533	39.0	35.0	39.0	32.0	39.0
12	36.7507046630069	39.0	35.0	39.0	33.0	39.0
13	36.778349050054764	39.0	35.0	39.0	33.0	39.0
14	37.68018547127024	40.0	37.0	41.0	33.0	41.0
15	37.90416142347437	40.0	37.0	41.0	33.0	41.0
16	37.88335761179294	40.0	37.0	41.0	33.0	41.0
17	37.840850840637074	39.0	36.0	41.0	33.0	41.0
18	37.786573607808485	39.0	36.0	41.0	33.0	41.0
19	37.61081886359179	39.0	36.0	41.0	33.0	41.0
20	37.65643518753182	39.0	36.0	41.0	33.0	41.0
21	37.5621789348184	39.0	35.0	41.0	33.0	41.0
22	37.71745162122077	39.0	35.0	41.0	33.0	41.0
23	37.77269191185707	39.0	35.0	41.0	33.0	41.0
24	37.756253815317685	40.0	35.0	41.0	33.0	41.0
25	37.35495181405271	39.0	35.0	41.0	33.0	41.0
26	37.4483684214006	39.0	35.0	41.0	33.0	41.0
27	37.50862454629399	39.0	35.0	41.0	33.0	41.0
28	37.39787069461371	39.0	35.0	41.0	33.0	41.0
29	37.33745148899315	39.0	35.0	41.0	33.0	41.0
30	37.03963963566884	39.0	35.0	41.0	32.0	41.0
31	36.97107961645176	39.0	35.0	41.0	32.0	41.0
32	36.89007036712998	39.0	35.0	41.0	31.0	41.0
33	36.807141613573606	39.0	35.0	41.0	31.0	41.0
34	36.62637103509982	39.0	35.0	41.0	31.0	41.0
35	36.544303964845085	39.0	35.0	41.0	31.0	41.0
36	36.367502418663484	39.0	35.0	41.0	30.0	41.0
37	36.353307784019414	39.0	35.0	41.0	30.0	41.0
38	36.19751720611861	39.0	35.0	41.0	30.0	41.0
39	36.13037202239936	39.0	35.0	41.0	30.0	41.0
40	36.025292939265654	39.0	35.0	41.0	29.0	41.0
41	35.78105530860824	38.0	35.0	40.0	28.0	41.0
42	35.80394170525142	38.0	35.0	40.0	29.0	41.0
43	35.83193429168971	38.0	35.0	40.0	29.0	41.0
44	35.834697848877056	38.0	35.0	40.0	29.0	41.0
45	35.82290755306184	38.0	35.0	40.0	29.0	41.0
46	35.70352364560354	38.0	35.0	40.0	29.0	41.0
47	35.640757399787994	38.0	35.0	40.0	28.0	41.0
48	35.545315506112225	38.0	35.0	40.0	28.0	41.0
49	35.50732871565112	38.0	35.0	40.0	28.0	41.0
50	35.39190722910418	38.0	35.0	40.0	28.0	41.0
51	34.079847850089145	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	16.0
9	31.0
10	31.0
11	19.0
12	25.0
13	21.0
14	25.0
15	31.0
16	66.0
17	97.0
18	193.0
19	320.0
20	589.0
21	934.0
22	1360.0
23	2034.0
24	2933.0
25	4231.0
26	5337.0
27	5892.0
28	6156.0
29	6841.0
30	8005.0
31	10022.0
32	13305.0
33	19505.0
34	33292.0
35	34512.0
36	38224.0
37	57508.0
38	97448.0
39	104740.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.43027527585986	21.331840630461276	24.4473436573718	14.79054043630706
2	35.78189495397377	23.151513014503166	24.16768224822209	16.898909783300976
3	30.87647075676069	24.18156614796711	28.198420761498845	16.743542333773355
4	27.295967278072474	24.561940925108484	28.852286325681025	19.289805471138017
5	24.464753626893334	29.285331770109064	27.348858324720172	18.901056278277427
6	24.498912427853305	32.70606021204902	27.423346548749016	15.371680811348657
7	76.00773972315945	6.71738330361885	11.979601686342871	5.295275286878833
8	77.64185268521231	5.012308187313642	12.256618543160196	5.089220584313838
9	72.36530964402121	7.436040399944464	13.235984423586764	6.962665532447556
10	40.54054649673949	22.457538406613146	21.135923378503758	15.865991718143613
11	30.351527118782272	24.081513918058544	26.047297818464703	19.519661144694478
12	26.876364974667393	20.55257920985184	32.116104662566144	20.454951152914628
13	25.128095503599894	22.46987965083094	32.9381196792158	19.46390516635336
14	21.33448518279366	24.267734478130652	34.218083008090126	20.179697330985558
15	19.78786282707052	24.425746479990657	35.54719093447461	20.23919975846422
16	24.034573114158714	23.58169352723779	32.13880373675245	20.244929621851053
17	24.565687374246025	24.19236473665768	29.14517049649266	22.096777392603627
18	25.035315792605388	23.706428245581947	30.559785614957587	20.69847034685508
19	24.413845994494924	25.966418592965933	27.970107743469608	21.649627669069535
20	24.077106330837907	26.531471274652183	29.74636539338818	19.645057001121728
21	23.39128575930607	26.32717960697545	31.23987632310259	19.041658310615894
22	22.32883685977041	23.215863787924533	31.45584809691403	22.99945125539103
23	21.09603471415695	25.506046107769915	30.596588968249943	22.80133020982319
24	22.438585781564385	24.764689937257998	30.337422839676222	22.4593014415014
25	23.411781039882055	25.88002988344136	27.639318322560456	23.068870754116137
26	21.372831191613244	25.01922809925005	29.24015400109749	24.36778670803922
27	22.29159274775599	24.52954515903677	30.16927338720874	23.009588705998507
28	20.951025094597842	24.414727511939052	30.231640746380823	24.402606647082287
29	22.718026811353063	24.60469452114871	29.74063553000135	22.936643137496887
30	25.047436657462157	22.7230955366568	30.228114676604307	22.001353129276737
31	24.49053801213409	23.84791179536454	28.848760255904516	22.812789936596857
32	25.439271161377196	23.676897411203647	29.280924182888423	21.602907244530737
33	25.146827749287624	23.72802542296309	28.581880849694663	22.54326597805462
34	23.73463680379405	23.338835471380435	30.245304266764812	22.681223458060707
35	23.7315514927396	24.839839299369935	30.071424950910497	21.357184256979966
36	25.1507945777862	25.694250082091312	28.210541626355607	20.94441371376688
37	22.945899070660232	26.285968666462452	29.016468949649926	21.75166331322739
38	23.243190828692512	26.087186482811514	28.349601003166853	22.320021685329124
39	24.63268269999978	24.244815024583318	27.641522116170776	23.480980159246126
40	25.63584955141781	22.233412596443518	30.49102725431558	21.639710597823093
41	22.463709028722043	24.36778670803922	28.993108737380524	24.175395525858214
42	22.32354775510564	24.77041980064483	30.2109250864438	22.69510735780573
43	22.430431745206196	23.735959079960246	30.130706999028128	23.70290217580543
44	22.631197343106425	22.918572029892257	29.806969717672	24.64326090932932
45	23.370349720008022	22.249059531076796	28.85162518759793	25.52896556131725
46	23.84703027792041	22.89256726529047	29.11189321297682	24.1485092438123
47	21.529741296668085	24.37836491736876	31.090679495683872	23.001214290279286
48	21.66681725923004	24.55554992363855	30.452681245496	23.324951571635413
49	23.68152537778532	23.02699867552004	30.364749880444197	22.926726066250446
50	21.63552338996348	22.61378737358489	30.92539497490981	24.82529426154182
51	21.750120657700165	22.69025901186302	28.859779223956117	26.699841106480697
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	196.0
1	402.5
2	609.0
3	3130.5
4	5652.0
5	3802.5
6	1953.0
7	2146.0
8	2339.0
9	2318.5
10	2298.0
11	2064.0
12	1830.0
13	1819.5
14	1809.0
15	1769.0
16	1729.0
17	1593.5
18	1458.0
19	1500.5
20	1543.0
21	1514.5
22	1486.0
23	1536.5
24	1587.0
25	1898.5
26	2600.0
27	2990.0
28	3699.5
29	4409.0
30	5000.5
31	5592.0
32	6498.0
33	7404.0
34	8340.5
35	9277.0
36	10473.5
37	11670.0
38	12920.5
39	14171.0
40	15659.0
41	17147.0
42	19334.0
43	21521.0
44	24489.5
45	27458.0
46	33205.0
47	38952.0
48	41884.5
49	44817.0
50	44721.0
51	44625.0
52	40230.0
53	35835.0
54	32491.5
55	29148.0
56	26732.5
57	24317.0
58	22841.5
59	21366.0
60	19331.0
61	17296.0
62	15651.0
63	14006.0
64	12212.0
65	10418.0
66	9271.5
67	8125.0
68	7095.5
69	6066.0
70	5027.5
71	3989.0
72	3321.0
73	2653.0
74	2156.0
75	1357.5
76	1056.0
77	831.0
78	606.0
79	438.0
80	270.0
81	190.5
82	111.0
83	80.5
84	50.0
85	36.5
86	23.0
87	24.5
88	26.0
89	17.0
90	8.0
91	5.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	453763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.3213965008156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.26097209816471	29.53984704005288
2	9.704333469887462	7.825845552309361
3	3.7560672002915627	4.543496245999934
4	2.244583114050276	3.6201890288262635
5	1.5979361636597718	3.221550881895891
6	1.2757064501353363	3.086295935673291
7	1.0359313086832456	2.9239137931518164
8	0.9241473192767977	2.981032838858047
9	0.7995260706470757	2.901420693656986
>10	5.324732289652035	33.61862184896417
>50	0.044095371334352935	1.1950294797252778
>100	0.02535483851725271	1.7897661149763056
>500	0.004960729275114661	1.5146226342435765
>1k	0.0016535764250382205	1.238367911666208
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3433	0.7565623464231328	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	1133	0.24968981604934737	TruSeq Adapter, Index 21 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTG	1006	0.2217016371982731	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTC	999	0.22015898167104855	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	925	0.2038509089546746	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTT	867	0.1910689060148139	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGC	824	0.18159259349043444	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCG	814	0.17938879988011364	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTC	779	0.1716755222439908	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT	564	0.12429395962209348	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT	525	0.11569916454184234	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCT	518	0.11415650901461777	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2037936103208063E-4	0.0	0.0	0.13178685789718422	0.0
2	2.2037936103208063E-4	0.0	0.0	0.729896443738251	0.0
3	2.2037936103208063E-4	0.0	0.0	1.1814537544929842	0.0
4	2.2037936103208063E-4	0.0	0.0	1.7063973924714002	0.0
5	4.4075872206416125E-4	0.0	0.0	3.1505433453146248	0.0
6	4.4075872206416125E-4	0.0	0.0	4.331997099807609	0.0
7	4.4075872206416125E-4	0.0	0.0	5.3655763030480665	0.0
8	4.4075872206416125E-4	0.0	0.0	6.739421239722057	0.0
9	4.4075872206416125E-4	0.0	0.0	7.402542737067588	0.0
10	4.4075872206416125E-4	0.0	0.0	8.644821195205427	0.0
11	4.4075872206416125E-4	0.0	0.0	10.135687572587452	0.0
12	4.4075872206416125E-4	0.0	0.0	11.393392585997535	0.0
13	4.4075872206416125E-4	0.0	0.0	11.96042868193308	0.0
14	4.4075872206416125E-4	0.0	0.0	12.233478710251827	0.0
15	4.4075872206416125E-4	0.0	0.0	12.587628343430382	0.0
16	6.611380830962418E-4	0.0	0.0	13.237086320391922	0.0
17	6.611380830962418E-4	0.0	0.0	13.959930624577147	0.0
18	6.611380830962418E-4	0.0	0.0	14.75572049726399	0.0
19	6.611380830962418E-4	0.0	0.0	15.281325273325503	0.0
20	6.611380830962418E-4	0.0	0.0	15.821915845937196	0.0
21	6.611380830962418E-4	0.0	0.0	16.427518330053353	0.0
22	6.611380830962418E-4	0.0	0.0	17.053616094745497	0.0
23	6.611380830962418E-4	0.0	0.0	17.685223343463438	0.0
24	6.611380830962418E-4	0.0	0.0	18.19165511511516	0.0
25	6.611380830962418E-4	0.0	0.0	18.6480607718126	0.0
26	6.611380830962418E-4	0.0	0.0	19.10380529042694	0.0
27	6.611380830962418E-4	0.0	0.0	19.61618730482653	0.0
28	6.611380830962418E-4	0.0	0.0	20.077220928105643	0.0
29	6.611380830962418E-4	0.0	0.0	20.565801971513764	0.0
30	6.611380830962418E-4	0.0	0.0	21.126006307257313	0.0
31	6.611380830962418E-4	0.0	0.0	21.59827927794906	0.0
32	6.611380830962418E-4	0.0	0.0	22.083113872219638	0.0
33	6.611380830962418E-4	0.0	0.0	22.580289710708012	0.0
34	6.611380830962418E-4	0.0	0.0	23.075041376225034	0.0
35	6.611380830962418E-4	0.0	0.0	23.57794707810024	0.0
36	6.611380830962418E-4	0.0	0.0	24.063883569175978	0.0
37	6.611380830962418E-4	0.0	0.0	24.584419619933755	0.0
38	6.611380830962418E-4	0.0	0.0	25.1274343655168	0.0
39	6.611380830962418E-4	0.0	0.0	25.931598653922862	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATG	20	7.028768E-4	45.000004	1
CTCACGA	35	1.2097553E-7	45.0	24
CCATACG	50	2.1827873E-11	45.0	2
CAACGAG	85	0.0	42.35294	14
CGTTTTT	1870	0.0	41.631016	1
AACACGT	60	3.6379788E-12	41.249996	41
TCACGAC	55	6.002665E-11	40.909092	25
CATACGG	55	6.002665E-11	40.909092	3
ACAACGA	90	0.0	40.0	13
TATTGCG	40	3.45206E-7	39.375004	1
AATTATC	80	0.0	39.375004	45
CGGTCTA	40	3.45206E-7	39.375004	31
ACGGTCT	40	3.45206E-7	39.375004	30
ATAGCGG	70	0.0	38.571426	2
TACGGGT	70	0.0	38.571426	4
TACGGGA	135	0.0	38.333332	4
CGCACGG	65	9.094947E-12	38.076927	2
ATTGCGG	95	0.0	37.894737	2
AACGTAG	30	1.1388333E-4	37.499996	1
TCCGAAC	60	1.546141E-10	37.499996	17
>>END_MODULE