SRR2935508.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935508.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357618
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	936	0.2617317920238914	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	935	0.2614521640409599	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	915	0.25585960438232974	RNA PCR Primer, Index 33 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC	824	0.23041345793556253	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG	720	0.2013321477106857	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	546	0.15267687868060334	Illumina Paired End PCR Primer 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	500	0.13981399146575396	TruSeq Adapter, Index 1 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	20	7.0267625E-4	45.0	2
ACGGGTA	45	3.8380676E-10	45.0	5
AACGGGC	50	2.1827873E-11	45.0	4
CCACTGG	25	3.8853985E-5	45.0	2
GCTTACG	20	7.0267625E-4	45.0	1
TACACCG	20	7.0267625E-4	45.0	15
TCGCGCA	20	7.0267625E-4	45.0	34
GCATCGA	20	7.0267625E-4	45.0	42
TGCGTAG	20	7.0267625E-4	45.0	1
CTTACGG	20	7.0267625E-4	45.0	2
TGGTACG	25	3.8853985E-5	45.0	1
GGCGATA	95	0.0	42.63158	8
TAGGGCG	85	0.0	42.35294	5
TTACGGG	80	0.0	42.1875	3
CCCTACA	125	0.0	41.399998	27
CGCATGG	60	3.6379788E-12	41.249996	2
AACGGGT	50	1.0768417E-9	40.5	4
TAGTTAG	45	1.9219442E-8	40.0	1
ATTGGGT	45	1.9219442E-8	40.0	4
GCCCTAC	130	0.0	39.807693	26