##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935506.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	418483
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.148947508023024	31.0	31.0	33.0	28.0	34.0
2	31.159726918417235	31.0	31.0	34.0	28.0	34.0
3	30.967339175068044	31.0	31.0	34.0	27.0	34.0
4	34.835140256593455	35.0	35.0	37.0	32.0	37.0
5	34.83893730450222	35.0	35.0	37.0	33.0	37.0
6	35.019632816625766	36.0	35.0	37.0	32.0	37.0
7	35.48775696981717	37.0	35.0	37.0	33.0	37.0
8	35.222085484953986	37.0	35.0	37.0	32.0	37.0
9	37.01072922914432	39.0	37.0	39.0	32.0	39.0
10	36.35858565341961	38.0	35.0	39.0	32.0	39.0
11	36.65653562988222	39.0	35.0	39.0	32.0	39.0
12	36.76947450673026	39.0	35.0	39.0	33.0	39.0
13	36.808587206648774	39.0	37.0	39.0	33.0	39.0
14	37.72582637765453	40.0	37.0	41.0	33.0	41.0
15	37.96487551465651	40.0	37.0	41.0	33.0	41.0
16	37.91399172726252	39.0	37.0	41.0	33.0	41.0
17	37.889331227313896	39.0	37.0	41.0	33.0	41.0
18	37.87947419608443	39.0	37.0	41.0	33.0	41.0
19	37.73256739222382	39.0	36.0	41.0	33.0	41.0
20	37.859640654459085	39.0	36.0	41.0	33.0	41.0
21	37.78409397753314	39.0	36.0	41.0	33.0	41.0
22	37.91904569600199	40.0	36.0	41.0	33.0	41.0
23	37.98959814377167	40.0	37.0	41.0	34.0	41.0
24	37.988259977107795	40.0	36.0	41.0	34.0	41.0
25	37.573796307137926	39.0	36.0	41.0	33.0	41.0
26	37.686171242320476	39.0	36.0	41.0	33.0	41.0
27	37.751519177601004	40.0	36.0	41.0	33.0	41.0
28	37.661816609037885	40.0	36.0	41.0	33.0	41.0
29	37.66654081527804	40.0	36.0	41.0	33.0	41.0
30	37.3901616075205	39.0	36.0	41.0	32.0	41.0
31	37.37424698255365	39.0	36.0	41.0	33.0	41.0
32	37.39133489293472	39.0	36.0	41.0	33.0	41.0
33	37.40728058248483	40.0	36.0	41.0	33.0	41.0
34	37.3205267597489	40.0	35.0	41.0	33.0	41.0
35	37.35252328051558	40.0	36.0	41.0	33.0	41.0
36	37.22696740369382	40.0	35.0	41.0	32.0	41.0
37	37.22167925578817	40.0	35.0	41.0	32.0	41.0
38	37.051225497809945	39.0	35.0	41.0	32.0	41.0
39	36.99097215418548	39.0	35.0	41.0	32.0	41.0
40	36.88692730648557	39.0	35.0	41.0	31.0	41.0
41	36.742297775536876	39.0	35.0	41.0	31.0	41.0
42	36.79861308583622	39.0	35.0	41.0	31.0	41.0
43	36.77852624837807	39.0	35.0	41.0	31.0	41.0
44	36.742868885952355	39.0	35.0	41.0	31.0	41.0
45	36.69536874855132	39.0	35.0	41.0	31.0	41.0
46	36.582836100869095	39.0	35.0	40.0	31.0	41.0
47	36.57552397588432	39.0	35.0	40.0	31.0	41.0
48	36.49802739896244	39.0	35.0	40.0	31.0	41.0
49	36.49793181562931	39.0	35.0	40.0	31.0	41.0
50	36.394075745012344	39.0	35.0	40.0	31.0	41.0
51	35.2688329036066	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	28.0
10	21.0
11	17.0
12	21.0
13	15.0
14	21.0
15	28.0
16	43.0
17	70.0
18	129.0
19	189.0
20	372.0
21	559.0
22	873.0
23	1167.0
24	1725.0
25	2390.0
26	3056.0
27	3520.0
28	4212.0
29	5288.0
30	6486.0
31	8426.0
32	11587.0
33	16481.0
34	28804.0
35	31498.0
36	35449.0
37	55285.0
38	95666.0
39	105030.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.45580824071706	20.855805373217073	22.926140368903873	11.762246017161987
2	34.593758886263004	22.82745057744281	23.91327724184734	18.665513294446846
3	33.25726493071403	22.794952244177182	26.363555986742593	17.58422683836619
4	29.050881397810663	25.831873696183592	24.785475156696926	20.331769749308812
5	24.216993282881262	30.06286993736902	25.329344322230536	20.390792457519183
6	25.557071613422767	33.456078263633174	25.32217557224547	15.664674550698596
7	78.86007316904151	6.102517903953088	10.002317895828504	5.0350910311768935
8	78.11524004559325	5.608352071649267	10.206388312070025	6.070019570687459
9	71.16991610172934	7.703538733950961	13.266488722361483	7.860056441958216
10	37.75278804634836	25.3883670304409	20.927970789733394	15.930874133477346
11	29.73191264639185	25.741069529706106	24.7125928651821	19.814424958719947
12	26.13248327889066	22.838920577418914	30.38498577003128	20.643610373659147
13	24.84808223989983	23.936934116798053	31.60415118415802	19.6108324591441
14	20.27776516608799	26.792247235849487	30.855016810718716	22.074970787343812
15	19.335313501384764	25.327671613900684	34.13639263721585	21.200622247498703
16	22.742620369286207	25.557549530088437	30.610562436227994	21.08926766439736
17	23.19210099334979	24.7900153650208	28.10890764977311	23.9089759918563
18	24.80220223999541	24.086044116487408	30.392871395014854	20.71888224850233
19	24.049961408229247	26.252679320306914	27.607334109151388	22.09002516231245
20	24.57495286546885	26.904557652282175	28.30246389936987	20.21802558287911
21	23.302977659785462	27.243400568242915	29.909458687688627	19.544163084282992
22	21.45845828862821	24.64401182365831	29.28004243899991	24.617487448713568
23	20.541814123871223	26.850553069061352	29.677669104838188	22.92996370222924
24	21.890255996061967	24.82800973994165	29.785200354614165	23.496533909382222
25	21.76647557965318	26.85126994405985	27.385341817947207	23.996912658339763
26	20.685189123572524	25.407722655400576	28.357663274254868	25.54942494677203
27	21.802319329578502	25.39816432208716	28.258017649462463	24.541498698871877
28	18.93434141888679	27.61975994245883	28.298162649378828	25.14773598927555
29	20.08683745815242	27.191785568350447	27.62549494244689	25.095882031050248
30	21.96027078758277	25.70713744644346	27.74592994219598	24.58666182377779
31	22.166730787152645	27.67567619234234	26.391992028350014	23.765600992154997
32	22.963417868826212	27.274943068177205	25.955893070925224	23.805745992071362
33	21.776272871299433	26.990343693770118	25.358497238836463	25.87488619609399
34	19.41584245955033	26.70813390269139	29.57181056339206	24.304213074366224
35	20.194607666261234	26.987715152108926	27.2197436932922	25.597933488337638
36	21.648191204899604	28.659706606958945	24.947727864692233	24.744374323449218
37	19.989342458355537	28.86138743987211	27.242205776578736	23.907064325193616
38	21.303852247283643	30.263833895283682	25.22372473911724	23.20858911831544
39	21.5007539135401	25.64763682156742	27.070633693602847	25.780975571289634
40	23.670973492352136	23.701321200622246	29.247783064067118	23.379922242958497
41	20.693552665221766	24.046854949902386	27.35690577633978	27.90268660853607
42	22.15000370385416	23.90586953352944	27.61091848414392	26.33320827847248
43	21.95764224592158	24.482714948994342	28.187763899608825	25.371878905475253
44	21.47805287192072	25.580728488373484	27.145666610113196	25.7955520295926
45	21.544005371783324	24.429427240772025	27.508644317690322	26.51792306975433
46	22.14880891218998	25.187403072526244	27.26490681819811	25.398881197085664
47	19.679413500667888	25.517882446837746	30.24256660366132	24.560137448833046
48	20.132956416389675	24.900414114790802	28.88910660648103	26.077522862338494
49	22.788978285856295	23.776354117132595	28.987557439609258	24.447110157401855
50	20.36641870756996	24.275538074425963	29.31206285559987	26.045980362404208
51	20.05935724987634	23.06879849360667	27.266101609862286	29.605742646654704
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	179.0
1	353.5
2	528.0
3	1554.5
4	2581.0
5	1649.0
6	717.0
7	688.5
8	660.0
9	721.5
10	783.0
11	763.5
12	744.0
13	690.0
14	636.0
15	686.5
16	737.0
17	742.0
18	747.0
19	792.5
20	838.0
21	992.0
22	1146.0
23	1197.0
24	1248.0
25	1805.5
26	2603.5
27	2844.0
28	3414.5
29	3985.0
30	4819.5
31	5654.0
32	6461.0
33	7268.0
34	8800.5
35	10333.0
36	11320.5
37	12308.0
38	13455.5
39	14603.0
40	16147.0
41	17691.0
42	20050.0
43	22409.0
44	24767.0
45	27125.0
46	32257.5
47	37390.0
48	41483.5
49	45577.0
50	45528.5
51	45480.0
52	39893.0
53	34306.0
54	30176.0
55	26046.0
56	23773.5
57	21501.0
58	19536.0
59	17571.0
60	16151.0
61	14731.0
62	13341.5
63	11952.0
64	10086.5
65	8221.0
66	7329.5
67	6438.0
68	5505.5
69	4573.0
70	3638.5
71	2704.0
72	2179.0
73	1654.0
74	1435.5
75	887.0
76	557.0
77	393.5
78	230.0
79	185.5
80	141.0
81	85.0
82	29.0
83	18.5
84	8.0
85	14.0
86	20.0
87	11.5
88	3.0
89	4.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	418483.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.0333580630041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94074092495222	27.74181316979525
2	10.489100572882556	7.978714356946075
3	3.8332374531051614	4.373726777833995
4	2.097914568041141	3.1916294380760526
5	1.3739465627131984	2.61279007895524
6	1.0607140384236486	2.4205510095493032
7	0.8587266599865409	2.286218097626098
8	0.7542327829249112	2.294880439667136
9	0.5920300021434813	2.02652001500077
>10	5.892108223391482	39.179108386076614
>50	0.06663350414642738	1.739733810206903
>100	0.03744168328227824	2.6373572896962862
>500	0.0012692096027890928	0.36035274398431005
>1k	0.0019038144041836394	1.1566043865859434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	1746	0.41722124913078906	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1738	0.41530958246810507	No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	1308	0.3125574993488386	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT	936	0.22366499953403127	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG	557	0.13309979138937542	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	460	0.1099208331043316	No Hit
TCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	458	0.1094429164386606	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	443	0.10585854144612802	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23274541618178038	0.0
2	0.0	0.0	0.0	1.1995708308342274	0.0
3	0.0	0.0	0.0	1.6707966631858402	0.0
4	0.0	0.0	0.0	2.250748536977607	0.0
5	2.389583328355035E-4	0.0	0.0	3.8295462420217787	0.0
6	2.389583328355035E-4	0.0	0.0	4.86519165653085	0.0
7	2.389583328355035E-4	0.0	0.0	5.825804154529575	0.0
8	2.389583328355035E-4	0.0	0.0	7.442835192827427	0.0
9	2.389583328355035E-4	0.0	0.0	8.05432956655348	0.0
10	2.389583328355035E-4	0.0	0.0	9.401576647080049	0.0
11	2.389583328355035E-4	0.0	0.0	11.112279351849418	0.0
12	2.389583328355035E-4	0.0	0.0	12.45618101571629	0.0
13	2.389583328355035E-4	0.0	0.0	13.04760288948416	0.0
14	2.389583328355035E-4	0.0	0.0	13.286083305653992	0.0
15	2.389583328355035E-4	0.0	0.0	13.758026013004113	0.0
16	2.389583328355035E-4	0.0	0.0	14.713859344346126	0.0
17	2.389583328355035E-4	0.0	0.0	15.7461593421955	0.0
18	2.389583328355035E-4	0.0	0.0	16.869980381520875	0.0
19	2.389583328355035E-4	0.0	0.0	17.514689963511064	0.0
20	2.389583328355035E-4	0.0	0.0	18.163939753825126	0.0
21	2.389583328355035E-4	0.0	0.0	18.91880912725248	0.0
22	2.389583328355035E-4	0.0	0.0	19.70474308394845	0.0
23	2.389583328355035E-4	0.0	0.0	20.487809540650396	0.0
24	2.389583328355035E-4	0.0	0.0	21.121527039330154	0.0
25	4.77916665671007E-4	0.0	0.0	21.675193496510012	0.0
26	4.77916665671007E-4	0.0	0.0	22.187520162109333	0.0
27	4.77916665671007E-4	0.0	0.0	22.787544535859283	0.0
28	4.77916665671007E-4	0.0	0.0	23.35459265967793	0.0
29	4.77916665671007E-4	0.0	0.0	23.94768724177565	0.0
30	4.77916665671007E-4	0.0	0.0	24.693715156888093	0.0
31	4.77916665671007E-4	0.0	0.0	25.247381614067955	0.0
32	4.77916665671007E-4	0.0	0.0	25.796268904591106	0.0
33	7.168749985065104E-4	0.0	0.0	26.346350986778436	0.0
34	7.168749985065104E-4	0.0	0.0	26.89834473562845	0.0
35	7.168749985065104E-4	0.0	0.0	27.515096192676882	0.0
36	7.168749985065104E-4	0.0	0.0	28.062310774870188	0.0
37	7.168749985065104E-4	0.0	0.0	28.601878690412754	0.0
38	7.168749985065104E-4	0.0	0.0	29.210744522477615	0.0
39	7.168749985065104E-4	0.0	0.0	29.987120145860167	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	30	2.16218E-6	45.000004	2
CTACGAA	55	1.8189894E-12	45.0	11
CCATACG	35	1.209446E-7	45.0	2
CGAATAT	55	1.8189894E-12	45.0	14
GCTACGA	55	1.8189894E-12	45.0	10
CGACTGG	25	3.8865393E-5	45.0	2
ATACGAG	20	7.02814E-4	45.0	1
CTTACGG	20	7.02814E-4	45.0	2
CGTTTTT	705	0.0	41.80851	1
GTTTGCG	50	1.0786607E-9	40.5	1
AACGGGA	120	0.0	39.375004	4
CATACGG	40	3.451205E-7	39.375	3
GCGTAAG	40	3.451205E-7	39.375	1
CGGGATA	115	0.0	39.130436	6
CACAACG	230	0.0	39.130436	12
CGACAGG	35	6.23877E-6	38.571426	2
TTGCGAG	35	6.23877E-6	38.571426	1
AGCTACG	65	9.094947E-12	38.07692	9
ATAACGT	95	0.0	37.894737	31
ATAGCGG	60	1.546141E-10	37.500004	2
>>END_MODULE