##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935505.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	443637
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.174164914107706	31.0	31.0	33.0	28.0	34.0
2	31.183014491577573	31.0	31.0	34.0	28.0	34.0
3	30.980959658459508	31.0	31.0	34.0	27.0	34.0
4	34.79440623753204	35.0	35.0	37.0	32.0	37.0
5	34.83232913395411	35.0	35.0	37.0	33.0	37.0
6	35.02468008754906	36.0	35.0	37.0	32.0	37.0
7	35.50032346265077	37.0	35.0	37.0	33.0	37.0
8	35.26654900290102	37.0	35.0	37.0	33.0	37.0
9	37.17322946462987	39.0	37.0	39.0	34.0	39.0
10	36.22260541839387	37.0	35.0	39.0	32.0	39.0
11	36.643390880381936	38.0	35.0	39.0	32.0	39.0
12	36.80653101522191	39.0	37.0	39.0	33.0	39.0
13	36.93890275157392	39.0	37.0	39.0	33.0	39.0
14	37.98149162490955	40.0	37.0	41.0	33.0	41.0
15	38.18151777241303	40.0	37.0	41.0	34.0	41.0
16	38.07310706726445	40.0	37.0	41.0	34.0	41.0
17	38.032003642617724	40.0	37.0	41.0	33.0	41.0
18	38.00624609759781	39.0	37.0	41.0	34.0	41.0
19	37.880670458054674	39.0	37.0	41.0	33.0	41.0
20	38.02061595403449	39.0	37.0	41.0	34.0	41.0
21	37.923559126042235	39.0	37.0	41.0	34.0	41.0
22	38.039566131769895	40.0	37.0	41.0	34.0	41.0
23	38.10018325793385	40.0	37.0	41.0	34.0	41.0
24	38.091072205429214	40.0	37.0	41.0	34.0	41.0
25	37.6563654519348	39.0	36.0	41.0	33.0	41.0
26	37.794633901139896	40.0	36.0	41.0	33.0	41.0
27	37.87760939687177	40.0	36.0	41.0	33.0	41.0
28	37.80574208192734	40.0	36.0	41.0	33.0	41.0
29	37.799069960350465	40.0	36.0	41.0	33.0	41.0
30	37.522864413924	40.0	36.0	41.0	33.0	41.0
31	37.5075365670582	40.0	36.0	41.0	33.0	41.0
32	37.53860701429322	40.0	36.0	41.0	33.0	41.0
33	37.53742812254163	40.0	36.0	41.0	33.0	41.0
34	37.43485777786794	40.0	36.0	41.0	33.0	41.0
35	37.45963929969773	40.0	36.0	41.0	33.0	41.0
36	37.31904913251149	40.0	36.0	41.0	33.0	41.0
37	37.3342214468135	40.0	36.0	41.0	33.0	41.0
38	37.182987442436044	40.0	36.0	41.0	32.0	41.0
39	37.11169942993934	40.0	36.0	41.0	32.0	41.0
40	37.0517765650746	39.0	35.0	41.0	32.0	41.0
41	36.90878127838751	39.0	35.0	41.0	31.0	41.0
42	36.952294330725344	39.0	35.0	41.0	31.0	41.0
43	36.92461855075208	39.0	35.0	41.0	31.0	41.0
44	36.90160649359724	39.0	35.0	41.0	31.0	41.0
45	36.87969668895967	39.0	35.0	41.0	31.0	41.0
46	36.76382943712991	39.0	35.0	41.0	31.0	41.0
47	36.77317266143266	39.0	35.0	41.0	31.0	41.0
48	36.688779339865704	39.0	35.0	41.0	31.0	41.0
49	36.65872548953311	39.0	35.0	41.0	31.0	41.0
50	36.54735741157749	39.0	35.0	41.0	31.0	41.0
51	35.402644504403376	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	23.0
10	29.0
11	20.0
12	10.0
13	11.0
14	12.0
15	27.0
16	46.0
17	69.0
18	117.0
19	207.0
20	361.0
21	554.0
22	874.0
23	1213.0
24	1866.0
25	2716.0
26	3502.0
27	4232.0
28	4540.0
29	5298.0
30	6452.0
31	8313.0
32	11126.0
33	15962.0
34	27307.0
35	31777.0
36	36190.0
37	56076.0
38	102780.0
39	121898.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.202746840322156	21.202694996134227	24.37353061173888	12.221027551804742
2	35.09806440851417	22.96134001447129	24.540333651160743	17.400261925853794
3	33.73433685648401	22.064660972822374	27.16725611254246	17.03374605815115
4	29.19729418420916	25.082668938794555	26.08123308019845	19.63880379679783
5	23.220786363626118	31.24108223615253	26.13916332497064	19.39896807525071
6	23.339577176836016	36.00353442115964	26.346314667171583	14.310573734832758
7	79.29681248408045	6.332654850700009	9.585088709913736	4.785443955305802
8	79.93810254780372	5.33273825221972	9.407916832906182	5.321242367070375
9	74.36079497426951	6.99513340862011	12.26182667360928	6.382244943501106
10	36.93425029923113	22.494291504090054	23.72953563386282	16.841922562815995
11	29.39655619346448	26.25682709061688	23.653121809046585	20.693494906872058
12	24.729903051368574	22.67371747622493	30.6678658452744	21.928513627132094
13	24.473161616366536	23.606236630398275	32.296900393790416	19.62370135944477
14	22.08246832432822	25.9529750674538	30.619402800036966	21.345153808181013
15	21.133494275725422	25.648446815752518	33.298845677885296	19.91921323063676
16	22.58152498551744	24.665886749752612	31.360549277900624	21.392038986829323
17	22.54681192055667	25.198529428338933	28.96219206242942	23.292466588674976
18	22.92234416876861	25.094841052482096	31.212680637548267	20.770134141201027
19	22.769291109623406	27.971742663483884	28.50686484671026	20.75210138018245
20	23.105827512132667	26.918854829511513	29.349896424328897	20.62542123402692
21	22.993573574792002	27.797275700629122	29.662314008975805	19.54683671560307
22	21.43554302278665	26.0221757878626	28.71649569355126	23.825785495799494
23	20.404069092523844	27.174243807437158	29.484465903430056	22.937221196608938
24	21.241240022811443	25.720577859826843	30.101862558803706	22.93631955855801
25	21.2360556040186	27.128034857327048	27.858812497605022	23.777097041049327
26	19.998331969605783	26.89653928775102	29.608441135432795	23.4966876072104
27	21.50587079075911	25.66377466261831	29.637293553062527	23.19306099356005
28	19.808537159885223	25.360373458480694	31.370241886948115	23.46084749468597
29	20.977060073889238	25.596151808798634	29.687334464889087	23.73945365242304
30	22.03918969788363	26.158097724040154	29.216679402304134	22.586033175772084
31	21.776136796525087	27.26598547911919	27.9816606820441	22.97621704231162
32	21.631874708376444	26.92967448612266	28.46741818198212	22.971032623518777
33	20.483413241005596	27.48192779231669	28.37793060542741	23.656728361250302
34	20.528044324526583	26.63033065321423	29.769834346549096	23.071790675710098
35	20.439232976510073	26.51672425879717	28.3526847400014	24.691358024691358
36	20.96128140799798	28.59747947082863	27.9613738258982	22.479865295275193
37	19.735955296785434	28.483196847873376	29.532252720129293	22.248595135211897
38	19.04710382587566	28.995327260801062	28.62655729797109	23.33101161535219
39	20.64255235699457	28.1151031135816	28.631065488225733	22.611279041198095
40	21.18466223511565	27.06920297450393	28.73948746384995	23.006647326530473
41	19.853619062431672	27.046662023230706	27.566456359591285	25.533262554746333
42	20.739027628443978	26.57871187479854	28.661721181957322	24.020539314800164
43	20.84519550894087	25.389676695135886	29.86518257043484	23.899945225488405
44	20.231405405770932	26.26110987135879	29.08458942784303	24.422895295027242
45	20.83640453794431	25.227832664994125	28.996003489339255	24.939759307722305
46	21.455153650394355	26.80254352094167	28.059652373449463	23.68265045521451
47	19.992245912762012	25.93223739228243	29.978338145826427	24.09717854912913
48	18.98331293377243	25.499451127836497	30.198563239765846	25.318672698625228
49	20.649990870914735	24.786706248577104	30.705509233900692	23.857793646607476
50	19.86443871904282	23.849002675610915	31.12657420368454	25.159984401661717
51	19.967901685386927	24.06922776955033	28.285287295694456	27.677583249368286
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	159.0
1	350.0
2	541.0
3	1897.0
4	3253.0
5	2172.0
6	1091.0
7	1019.5
8	948.0
9	951.5
10	955.0
11	999.5
12	1044.0
13	1047.0
14	1050.0
15	1029.5
16	1009.0
17	981.5
18	954.0
19	1091.0
20	1228.0
21	1297.0
22	1366.0
23	1612.5
24	1859.0
25	2099.5
26	3077.5
27	3815.0
28	4341.0
29	4867.0
30	5970.0
31	7073.0
32	7756.0
33	8439.0
34	9509.5
35	10580.0
36	11910.5
37	13241.0
38	15275.5
39	17310.0
40	19594.0
41	21878.0
42	24796.5
43	27715.0
44	33324.5
45	38934.0
46	43867.0
47	48800.0
48	49276.5
49	49753.0
50	44215.5
51	38678.0
52	34086.5
53	29495.0
54	26678.5
55	23862.0
56	21775.5
57	19689.0
58	18247.5
59	16806.0
60	15426.5
61	14047.0
62	12410.0
63	10773.0
64	9338.0
65	7903.0
66	6299.0
67	4695.0
68	3791.5
69	2888.0
70	2458.5
71	2029.0
72	1618.5
73	1208.0
74	977.5
75	534.0
76	321.0
77	242.5
78	164.0
79	117.5
80	71.0
81	44.5
82	18.0
83	24.0
84	30.0
85	17.5
86	5.0
87	2.5
88	0.0
89	1.5
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	443637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.508288653364055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.85595909162242	29.51270221001125
2	10.363562871980037	8.396203925909079
3	3.90754707439776	4.748641344489381
4	2.2432093125104866	3.6347428136435656
5	1.5494016682702216	3.138180500914698
6	1.1662652366347952	2.834604531119173
7	0.9684032515385003	2.7459850912324524
8	0.8651162183128454	2.803550199209877
9	0.7535773757567475	2.7473516873819026
>10	5.24314492021782	34.67370406423255
>50	0.05287530203546956	1.461952488497241
>100	0.028125160657164524	1.9400036607022237
>500	0.001125006426286581	0.3267518498293386
>1k	0.0016875096394298715	1.0356256328272968
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1992	0.4490157493626546	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1496	0.33721263104745547	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1057	0.23825785495799495	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	860	0.19385218094974044	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	574	0.12938506030831515	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC	469	0.10571706147142822	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	468	0.10549165195869595	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2540951273225632E-4	0.0	0.0	0.19993823779351136	0.0
2	2.2540951273225632E-4	0.0	0.0	0.9751215520797408	0.0
3	2.2540951273225632E-4	0.0	0.0	1.3610226378773638	0.0
4	2.2540951273225632E-4	0.0	0.0	1.8630096227320985	0.0
5	2.2540951273225632E-4	0.0	0.0	3.192700338339679	0.0
6	2.2540951273225632E-4	0.0	0.0	4.184502194361606	0.0
7	2.2540951273225632E-4	0.0	0.0	5.027984591005709	0.0
8	2.2540951273225632E-4	0.0	0.0	6.518843108216853	0.0
9	2.2540951273225632E-4	0.0	0.0	7.088227537378533	0.0
10	2.2540951273225632E-4	0.0	0.0	8.350520808679168	0.0
11	2.2540951273225632E-4	0.0	0.0	10.003674175057537	0.0
12	2.2540951273225632E-4	0.0	0.0	11.297975597166152	0.0
13	2.2540951273225632E-4	0.0	0.0	11.888773930037395	0.0
14	2.2540951273225632E-4	0.0	0.0	12.124777689868068	0.0
15	2.2540951273225632E-4	0.0	0.0	12.512256642254817	0.0
16	2.2540951273225632E-4	0.0	0.0	13.330267763960174	0.0
17	2.2540951273225632E-4	0.0	0.0	14.33874992392429	0.0
18	2.2540951273225632E-4	0.0	0.0	15.433338517752127	0.0
19	2.2540951273225632E-4	0.0	0.0	16.04690321140933	0.0
20	4.5081902546451264E-4	0.0	0.0	16.65641053383735	0.0
21	4.5081902546451264E-4	0.0	0.0	17.44489300937478	0.0
22	4.5081902546451264E-4	0.0	0.0	18.241490227370576	0.0
23	4.5081902546451264E-4	0.0	0.0	19.073702148378064	0.0
24	4.5081902546451264E-4	0.0	0.0	19.74654954388385	0.0
25	4.5081902546451264E-4	0.0	0.0	20.361241285104715	0.0
26	4.5081902546451264E-4	0.0	0.0	20.911691315196883	0.0
27	4.5081902546451264E-4	0.0	0.0	21.489190486816923	0.0
28	4.5081902546451264E-4	0.0	0.0	22.063759334771447	0.0
29	6.76228538196769E-4	0.0	0.0	22.666278962304766	0.0
30	6.76228538196769E-4	0.0	0.0	23.332364072428586	0.0
31	9.016380509290253E-4	0.0	0.0	23.93916648070382	0.0
32	9.016380509290253E-4	0.0	0.0	24.528161537473203	0.0
33	0.0011270475636612817	0.0	0.0	25.107689394707837	0.0
34	0.0011270475636612817	0.0	0.0	25.675045138254923	0.0
35	0.0011270475636612817	0.0	0.0	26.319716344669178	0.0
36	0.001352457076393538	0.0	0.0	26.885719631139875	0.0
37	0.001352457076393538	0.0	0.0	27.45578028883975	0.0
38	0.001352457076393538	0.0	0.0	28.026517175077824	0.0
39	0.001352457076393538	0.0	0.0	28.625204840894696	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACAC	30	2.1624764E-6	45.000004	34
CTTCGAA	20	7.0285983E-4	45.0	16
CGTATGG	40	6.7993824E-9	45.0	2
GCGCGAC	20	7.0285983E-4	45.0	9
CGCGACC	20	7.0285983E-4	45.0	10
CTACGGG	35	1.2096643E-7	45.0	3
AGCGCGT	20	7.0285983E-4	45.0	30
TTAACGG	45	3.8380676E-10	45.0	2
AGTACGG	45	3.8380676E-10	45.0	2
TACGTAG	20	7.0285983E-4	45.0	1
TAGGGCG	105	0.0	42.857143	5
CGTTTTT	1010	0.0	42.326733	1
AACGGGA	80	0.0	42.1875	4
CGGGTAT	55	6.002665E-11	40.909092	6
ATAGCGG	50	1.0786607E-9	40.500004	2
ACGGGCC	40	3.4518234E-7	39.375	5
ACGGGTA	105	0.0	38.57143	5
CCGTAGG	35	6.2396175E-6	38.571426	2
ATCCCGC	35	6.2396175E-6	38.571426	33
TGCGAGG	135	0.0	38.333336	2
>>END_MODULE