SRR2935504.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935504.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269850
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGC	10903	4.040392810820826	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC	9922	3.6768575134333887	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG	9420	3.49082823790995	No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	3898	1.4445062071521215	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTATGCTCAT	2177	0.8067444876783397	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT	2146	0.7952566240503984	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTATGCTCATCGTA	1673	0.6199740596627756	No Hit
GAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT	1635	0.6058921623123958	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTC	1566	0.5803224013340745	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	889	0.32944228274967574	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	767	0.2842319807300352	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	723	0.2679266259032796	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT	565	0.20937557902538448	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATG	462	0.17120622568093385	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTAT	424	0.157124328330554	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTATGCTCATCGT	411	0.15230683713173984	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTATGCTCA	410	0.15193626088567724	No Hit
TCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	340	0.1259959236612933	No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG	325	0.1204372799703539	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACACCCATCGAATGC	320	0.11858439874004076	No Hit
ACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	286	0.10598480637391144	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	35	1.2073178E-7	45.000004	14
CGTAAGG	30	2.1593078E-6	45.000004	2
GCTACGA	35	1.2073178E-7	45.000004	10
AGCTACG	35	1.2073178E-7	45.000004	9
GGCGAAC	155	0.0	45.000004	32
AGGGCGT	30	2.1593078E-6	45.000004	6
TGATCCG	25	3.882848E-5	45.0	4
ACAACGG	20	7.0236844E-4	45.0	2
CAGCGGT	20	7.0236844E-4	45.0	28
GCGACAA	20	7.0236844E-4	45.0	11
CTCAACG	50	2.1827873E-11	45.0	43
CAACGTA	25	3.882848E-5	45.0	45
GCGGTTA	20	7.0236844E-4	45.0	30
CGTGCGG	25	3.882848E-5	45.0	2
AACCCGC	20	7.0236844E-4	45.0	21
CGGCCAA	40	6.7811925E-9	45.0	31
TAGCGAG	20	7.0236844E-4	45.0	1
TCGATGG	20	7.0236844E-4	45.0	1
ACGGGTC	25	3.882848E-5	45.0	5
ACGGGCG	20	7.0236844E-4	45.0	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content