##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935504.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269850
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12135630906059	31.0	31.0	33.0	28.0	34.0
2	31.06912729294052	31.0	31.0	34.0	28.0	34.0
3	30.96834167129887	31.0	30.0	34.0	28.0	34.0
4	34.902175282564386	35.0	35.0	37.0	32.0	37.0
5	34.78669260700389	35.0	35.0	37.0	33.0	37.0
6	34.90553640911617	35.0	35.0	37.0	32.0	37.0
7	35.27712803409302	36.0	35.0	37.0	33.0	37.0
8	34.68606262738558	36.0	35.0	37.0	31.0	37.0
9	36.34408375023161	39.0	35.0	39.0	32.0	39.0
10	35.95362979433018	37.0	35.0	39.0	32.0	39.0
11	36.37833240689272	37.0	35.0	39.0	32.0	39.0
12	36.493740967204005	38.0	35.0	39.0	32.0	39.0
13	36.34882712618121	39.0	35.0	39.0	32.0	39.0
14	37.410491013526034	39.0	36.0	40.0	32.0	41.0
15	37.38673337039096	39.0	36.0	41.0	32.0	41.0
16	37.70390216787104	39.0	36.0	40.0	33.0	41.0
17	37.294400592921995	39.0	36.0	40.0	32.0	41.0
18	37.40903094311655	39.0	36.0	40.0	32.0	41.0
19	37.36100796738929	39.0	36.0	40.0	32.0	41.0
20	36.945818047063185	39.0	35.0	40.0	31.0	41.0
21	37.40469890680007	39.0	36.0	40.0	32.0	41.0
22	37.427711691680564	39.0	36.0	41.0	32.0	41.0
23	37.34249027237354	39.0	36.0	41.0	32.0	41.0
24	37.299618306466556	39.0	35.0	41.0	31.0	41.0
25	37.34462849731332	39.0	35.0	41.0	32.0	41.0
26	37.16811191402631	39.0	35.0	41.0	31.0	41.0
27	37.02273485269594	39.0	35.0	41.0	31.0	41.0
28	36.95468964239392	39.0	35.0	41.0	31.0	41.0
29	37.00100055586437	39.0	35.0	41.0	31.0	41.0
30	36.87716879748008	39.0	35.0	41.0	31.0	41.0
31	36.89875856957569	39.0	35.0	41.0	31.0	41.0
32	36.69597924773022	39.0	35.0	41.0	30.0	41.0
33	36.62122290161201	39.0	35.0	41.0	30.0	41.0
34	36.492047433759495	39.0	35.0	41.0	30.0	41.0
35	36.44449508986474	39.0	35.0	41.0	30.0	41.0
36	36.41908838243469	39.0	35.0	41.0	30.0	41.0
37	36.300974615527146	39.0	35.0	41.0	30.0	41.0
38	36.25756531406337	39.0	35.0	41.0	30.0	41.0
39	36.101385955160275	39.0	35.0	41.0	29.0	41.0
40	36.23342597739485	39.0	35.0	41.0	29.0	41.0
41	35.88632944228275	39.0	35.0	40.0	27.0	41.0
42	36.15642764498796	39.0	35.0	41.0	29.0	41.0
43	36.04320177876598	39.0	35.0	41.0	29.0	41.0
44	36.19675004632203	39.0	35.0	41.0	29.0	41.0
45	36.234656290531774	39.0	35.0	40.0	30.0	41.0
46	36.088912358717806	39.0	35.0	40.0	29.0	41.0
47	35.847719103205485	39.0	35.0	40.0	29.0	41.0
48	35.9939670187141	39.0	35.0	40.0	29.0	41.0
49	36.04596627756161	39.0	35.0	40.0	29.0	41.0
50	35.86644802668149	39.0	35.0	40.0	29.0	41.0
51	34.69229571984436	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	16.0
10	7.0
11	10.0
12	7.0
13	5.0
14	7.0
15	8.0
16	13.0
17	29.0
18	90.0
19	224.0
20	546.0
21	1329.0
22	2251.0
23	2607.0
24	2706.0
25	2746.0
26	2771.0
27	2594.0
28	2695.0
29	3173.0
30	4130.0
31	5836.0
32	8086.0
33	11429.0
34	18932.0
35	21553.0
36	22511.0
37	32176.0
38	55884.0
39	65466.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	54.7029831387808	17.30776357235501	18.608856772280895	9.380396516583286
2	26.28237909949972	38.87381878821567	19.464887900685564	15.378914211599037
3	25.605336297943303	38.715953307393	22.464332036316474	13.214378358347231
4	24.097832128960533	19.263294422827496	40.78413933666852	15.85473411154345
5	35.35371502686678	23.666481378543637	22.027422642208634	18.952380952380953
6	19.54011487863628	43.09949972206781	24.402075226977953	12.958310172317955
7	64.85528997591254	5.004632203075783	25.34593292569946	4.79414489531221
8	61.597183620529925	19.149527515286273	12.212710765239947	7.0405780989438576
9	57.805818047063184	5.712062256809339	12.367611636094127	24.11450806003335
10	42.184176394293125	16.590327959977767	23.94219010561423	17.28330554011488
11	35.10505836575876	20.220863442653325	23.521215490087087	21.152862701500833
12	23.78247174356124	17.182508801185843	30.08375023161015	28.95126922364277
13	26.11858439874004	20.969427459699833	36.57402260515101	16.337965536409115
14	20.56142301278488	31.644987956272004	25.828793774319063	21.96479525662405
15	15.405225125069483	21.09023531591625	40.41652770057439	23.088011858439874
16	16.677413377802484	23.344821196961274	27.17917361497128	32.79859181026496
17	17.231424865666114	27.31888085973689	31.42894200481749	24.020752269779507
18	17.293311098758572	24.183805818047063	31.094682230869005	27.428200852325368
19	17.656846396146005	26.227904391328515	27.050213081341486	29.06503613118399
20	22.714100426162684	24.99870298313878	34.43283305540115	17.854363535297388
21	19.673522327218826	34.002223457476376	28.26829720214934	18.055957013155457
22	16.44469149527515	22.21863998517695	33.49861033907727	27.838058180470632
23	20.099685010190846	29.31739855475264	29.315175097276263	21.26774133778025
24	22.209375579025384	25.161756531406336	25.83361126551788	26.795256624050403
25	16.513989253288862	36.127848804891606	24.977950713359274	22.380211228460254
26	16.57698721511951	21.999258847507875	34.76264591439689	26.66110802297573
27	24.605521586066335	28.16379470075968	24.92088197146563	22.309801741708355
28	14.53585325180656	26.48656661108023	35.169168056327585	23.808412080785622
29	21.305169538632573	20.578469520103763	34.19937002038169	23.91699092088197
30	17.366685195478972	33.36816750046322	29.076523994811932	20.188623309245877
31	21.07059477487493	21.476375764313506	29.945895868074857	27.507133592736704
32	28.634055957013153	26.946081156197888	23.349268111914025	21.07059477487493
33	20.97461552714471	23.205113952195664	23.823976283120253	31.996294237539374
34	21.64461738002594	20.095238095238095	34.40355753196221	23.856586992773764
35	20.052992403186956	24.3272188252733	24.911246989068	30.708541782471745
36	17.586436909394106	28.587363350009266	33.25180655919955	20.57439318139707
37	19.68093385214008	27.22030757828423	32.71447100240874	20.384287567166943
38	18.064480266814897	23.462664443209192	26.880118584398737	31.592736705577174
39	25.58384287567167	23.012043727997035	30.194923105428945	21.209190290902352
40	18.01852881230313	19.93626088567723	29.659811006114506	32.38539929590513
41	16.523624235686494	27.736890865295532	28.688901241430425	27.050583657587552
42	18.330924587733925	21.594960163053546	32.4124513618677	27.661663887344822
43	22.38688160088938	26.54548823420419	25.498980915323326	25.568649249583103
44	21.08764128219381	21.27922920140819	31.49379284787845	26.139336668519547
45	18.69816564758199	18.872707059477488	29.359273670557716	33.06985362238281
46	28.82638502871966	23.906244209746156	26.01037613488975	21.25699462664443
47	15.491939966648138	22.43060959792477	39.331480452103015	22.74596998332407
48	19.99295905132481	24.675931072818234	27.70205669816565	27.62905317769131
49	19.023531591624977	18.535482675560498	37.46525847693163	24.975727255882898
50	22.31424865666111	19.497128034093013	31.0213081341486	27.16731517509728
51	19.590513248100798	19.358161941819528	27.660552158606635	33.390772651473036
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	76.0
1	173.5
2	271.0
3	758.0
4	1245.0
5	829.5
6	414.0
7	405.0
8	396.0
9	413.0
10	430.0
11	422.5
12	415.0
13	403.5
14	392.0
15	390.5
16	389.0
17	425.5
18	462.0
19	453.5
20	445.0
21	502.0
22	559.0
23	660.5
24	762.0
25	880.0
26	1197.0
27	1396.0
28	1643.0
29	1890.0
30	2117.0
31	2344.0
32	2806.0
33	3268.0
34	4107.0
35	4946.0
36	5550.5
37	6155.0
38	6929.5
39	7704.0
40	8900.0
41	10096.0
42	12463.0
43	14830.0
44	36741.5
45	58653.0
46	44214.0
47	29775.0
48	30283.5
49	30792.0
50	25558.0
51	20324.0
52	18669.5
53	17015.0
54	15257.0
55	13499.0
56	12479.0
57	11459.0
58	10095.0
59	8731.0
60	7605.0
61	6479.0
62	5756.5
63	5034.0
64	4033.5
65	3033.0
66	2542.5
67	2052.0
68	1650.0
69	1248.0
70	978.0
71	708.0
72	620.0
73	532.0
74	417.5
75	218.0
76	133.0
77	112.0
78	91.0
79	84.0
80	77.0
81	41.5
82	6.0
83	4.5
84	3.0
85	8.0
86	13.0
87	9.5
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	269850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.72114137483787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.20982521293344	27.25069483046137
2	10.553828765187905	7.750972762645915
3	3.8489484519436483	4.240133407448583
4	2.0657570742340448	3.0342783027607934
5	1.3886085657772576	2.5495645729108762
6	1.1433819077221168	2.519177320733741
7	0.876962822427643	2.2542153047989624
8	0.8224680095265006	2.416157124328331
9	0.6478827756024704	2.1411895497498614
>10	4.3454567472651675	25.08541782471744
>50	0.05651313930488839	1.427089123587178
>100	0.027247406450571183	2.179358903094312
>500	0.004036652807492028	1.0909764684083751
>1k	0.006054979211238041	4.852695942190106
>5k	0.002018326403746014	7.167685751343339
>10k+	0.001009163201873007	4.040392810820826
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGC	10903	4.040392810820826	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC	9922	3.6768575134333887	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG	9420	3.49082823790995	No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	3898	1.4445062071521215	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTATGCTCAT	2177	0.8067444876783397	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT	2146	0.7952566240503984	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTATGCTCATCGTA	1673	0.6199740596627756	No Hit
GAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT	1635	0.6058921623123958	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTC	1566	0.5803224013340745	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	889	0.32944228274967574	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	767	0.2842319807300352	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	723	0.2679266259032796	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT	565	0.20937557902538448	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATG	462	0.17120622568093385	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTAT	424	0.157124328330554	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTATGCTCATCGT	411	0.15230683713173984	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTATGCTCA	410	0.15193626088567724	No Hit
TCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	340	0.1259959236612933	No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG	325	0.1204372799703539	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACACCCATCGAATGC	320	0.11858439874004076	No Hit
ACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	286	0.10598480637391144	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2783027607930332	0.0
2	0.0	0.0	0.0	2.3457476375764315	0.0
3	0.0	0.0	0.0	2.848619603483417	0.0
4	0.0	0.0	0.0	4.0014823049842505	0.0
5	3.7057624606262737E-4	0.0	0.0	9.088011858439874	0.0
6	3.7057624606262737E-4	0.0	0.0	10.031498980915323	0.0
7	3.7057624606262737E-4	0.0	0.0	11.609042060403928	0.0
8	3.7057624606262737E-4	0.0	0.0	13.343709468223087	0.0
9	3.7057624606262737E-4	0.0	0.0	13.745043542708911	0.0
10	3.7057624606262737E-4	0.0	0.0	18.34871224754493	0.0
11	3.7057624606262737E-4	0.0	0.0	19.920696683342598	0.0
12	3.7057624606262737E-4	0.0	0.0	25.28775245506763	0.0
13	3.7057624606262737E-4	0.0	0.0	25.988141560125996	0.0
14	3.7057624606262737E-4	0.0	0.0	26.35019455252918	0.0
15	3.7057624606262737E-4	0.0	0.0	27.349638688160088	0.0
16	3.7057624606262737E-4	0.0	0.0	28.332777468964238	0.0
17	3.7057624606262737E-4	0.0	0.0	29.392625532703352	0.0
18	3.7057624606262737E-4	0.0	0.0	30.416898276820454	0.0
19	3.7057624606262737E-4	0.0	0.0	31.92514359829535	0.0
20	3.7057624606262737E-4	0.0	0.0	32.63516768575134	0.0
21	3.7057624606262737E-4	0.0	0.0	33.40559570131555	0.0
22	3.7057624606262737E-4	0.0	0.0	34.28126737076153	0.0
23	3.7057624606262737E-4	0.0	0.0	35.046877895126926	0.0
24	3.7057624606262737E-4	0.0	0.0	35.687233648323144	0.0
25	3.7057624606262737E-4	0.0	0.0	36.23716879748008	0.0
26	3.7057624606262737E-4	0.0	0.0	36.77598665925514	0.0
27	3.7057624606262737E-4	0.0	0.0	37.35816194181953	0.0
28	3.7057624606262737E-4	0.0	0.0	37.8621456364647	0.0
29	3.7057624606262737E-4	0.0	0.0	38.397257735779135	0.0
30	3.7057624606262737E-4	0.0	0.0	38.97980359458958	0.0
31	3.7057624606262737E-4	0.0	0.0	39.526403557531964	0.0
32	3.7057624606262737E-4	0.0	0.0	40.00111172873819	0.0
33	3.7057624606262737E-4	0.0	0.0	40.52399481193255	0.0
34	3.7057624606262737E-4	0.0	0.0	41.02093755790254	0.0
35	3.7057624606262737E-4	0.0	0.0	41.53715026866778	0.0
36	3.7057624606262737E-4	0.0	0.0	42.04187511580508	0.0
37	3.7057624606262737E-4	0.0	0.0	42.54030016675931	0.0
38	3.7057624606262737E-4	0.0	0.0	43.025755049101356	0.0
39	3.7057624606262737E-4	0.0	0.0	43.539373726144156	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	35	1.2073178E-7	45.000004	14
CGTAAGG	30	2.1593078E-6	45.000004	2
GCTACGA	35	1.2073178E-7	45.000004	10
AGCTACG	35	1.2073178E-7	45.000004	9
GGCGAAC	155	0.0	45.000004	32
AGGGCGT	30	2.1593078E-6	45.000004	6
TGATCCG	25	3.882848E-5	45.0	4
ACAACGG	20	7.0236844E-4	45.0	2
CAGCGGT	20	7.0236844E-4	45.0	28
GCGACAA	20	7.0236844E-4	45.0	11
CTCAACG	50	2.1827873E-11	45.0	43
CAACGTA	25	3.882848E-5	45.0	45
GCGGTTA	20	7.0236844E-4	45.0	30
CGTGCGG	25	3.882848E-5	45.0	2
AACCCGC	20	7.0236844E-4	45.0	21
CGGCCAA	40	6.7811925E-9	45.0	31
TAGCGAG	20	7.0236844E-4	45.0	1
TCGATGG	20	7.0236844E-4	45.0	1
ACGGGTC	25	3.882848E-5	45.0	5
ACGGGCG	20	7.0236844E-4	45.0	5
>>END_MODULE