##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935502.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	567833
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.295532313197718	31.0	31.0	33.0	28.0	34.0
2	31.291856936810646	31.0	31.0	34.0	28.0	34.0
3	31.098527207823427	31.0	31.0	34.0	28.0	34.0
4	34.89845429906328	35.0	35.0	37.0	32.0	37.0
5	34.92020365142568	35.0	35.0	37.0	33.0	37.0
6	35.10842096179687	37.0	35.0	37.0	33.0	37.0
7	35.59419935086548	37.0	35.0	37.0	33.0	37.0
8	35.3876738407243	37.0	35.0	37.0	33.0	37.0
9	37.2428988804807	39.0	37.0	39.0	34.0	39.0
10	36.4905984682116	38.0	35.0	39.0	32.0	39.0
11	36.77811081779326	39.0	37.0	39.0	33.0	39.0
12	36.90581737940556	39.0	37.0	39.0	33.0	39.0
13	36.97355032201369	39.0	37.0	39.0	33.0	39.0
14	38.004471385072726	40.0	37.0	41.0	33.0	41.0
15	38.21047209302735	40.0	37.0	41.0	34.0	41.0
16	38.14344006072208	40.0	37.0	41.0	34.0	41.0
17	38.06669566580315	40.0	37.0	41.0	34.0	41.0
18	38.05841330109381	40.0	37.0	41.0	34.0	41.0
19	37.95876076240726	39.0	37.0	41.0	34.0	41.0
20	38.07791727497345	40.0	37.0	41.0	34.0	41.0
21	38.00625183812846	40.0	37.0	41.0	34.0	41.0
22	38.135152412769244	40.0	37.0	41.0	34.0	41.0
23	38.197799352978784	40.0	37.0	41.0	34.0	41.0
24	38.190633513726745	40.0	37.0	41.0	34.0	41.0
25	37.78166115741776	40.0	36.0	41.0	33.0	41.0
26	37.906976170810786	40.0	36.0	41.0	34.0	41.0
27	37.98474727604771	40.0	37.0	41.0	34.0	41.0
28	37.906211861586065	40.0	36.0	41.0	34.0	41.0
29	37.91005630176478	40.0	36.0	41.0	34.0	41.0
30	37.617699217903855	40.0	36.0	41.0	33.0	41.0
31	37.62062613479667	40.0	36.0	41.0	33.0	41.0
32	37.6530494000877	40.0	36.0	41.0	33.0	41.0
33	37.66036669231975	40.0	36.0	41.0	33.0	41.0
34	37.601544820396136	40.0	36.0	41.0	33.0	41.0
35	37.6284911232704	40.0	36.0	41.0	33.0	41.0
36	37.48863838487724	40.0	36.0	41.0	33.0	41.0
37	37.51834782409617	40.0	36.0	41.0	33.0	41.0
38	37.38265652049106	40.0	36.0	41.0	33.0	41.0
39	37.34116016504853	40.0	36.0	41.0	33.0	41.0
40	37.26529631071107	40.0	35.0	41.0	33.0	41.0
41	37.12790204162139	39.0	35.0	41.0	32.0	41.0
42	37.181836913317824	39.0	35.0	41.0	32.0	41.0
43	37.14407052777841	39.0	35.0	41.0	32.0	41.0
44	37.112429182523734	39.0	35.0	41.0	32.0	41.0
45	37.087133012699155	39.0	35.0	41.0	32.0	41.0
46	36.986922211283954	39.0	35.0	41.0	32.0	41.0
47	36.98475432037236	39.0	35.0	41.0	32.0	41.0
48	36.92555205491755	39.0	35.0	41.0	32.0	41.0
49	36.92957612537489	39.0	35.0	41.0	32.0	41.0
50	36.84394531490773	39.0	35.0	41.0	32.0	41.0
51	35.76697374051878	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	14.0
9	24.0
10	24.0
11	25.0
12	26.0
13	18.0
14	16.0
15	27.0
16	49.0
17	78.0
18	135.0
19	240.0
20	391.0
21	651.0
22	950.0
23	1424.0
24	1983.0
25	2692.0
26	3609.0
27	4272.0
28	5004.0
29	5802.0
30	7572.0
31	9917.0
32	13673.0
33	20254.0
34	37070.0
35	40684.0
36	45256.0
37	71752.0
38	131274.0
39	162904.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56914797132255	20.860182483230105	24.191091394829115	11.379578150618228
2	34.1345078570636	23.234295998999706	24.91154969859096	17.719646445345727
3	33.748478866145504	23.032123881493327	26.57682804627417	16.642569206087003
4	29.243457143209362	25.291767121671338	25.73555957473412	19.72921616038518
5	24.799721044743787	30.230190918808873	24.839521479026406	20.13056655742093
6	25.282433391507713	34.65508344883091	25.184693386964124	14.877789772697254
7	79.76183138352297	6.29903510363082	9.25923643042937	4.679897082416837
8	80.77392472786893	5.242210297745992	8.850841708741832	5.133023265643244
9	73.92842613937547	7.546761107579165	11.39595620543364	7.1288565476117105
10	38.23994026412695	26.41022976825933	19.92540060193754	15.424429365676176
11	28.872221234060014	26.40494652477049	25.516481078063446	19.206351163106053
12	26.314955277343866	22.2371718445388	30.318949409421432	21.128923468695902
13	24.918770131359043	23.554108338190982	31.926288186843664	19.600833343606308
14	21.29182347626855	26.377825874861095	30.938673870662676	21.391676778207678
15	20.09710601532493	25.957808017498103	33.51742501756678	20.427660949610186
16	23.647093423594615	25.710728330336558	30.227901513297045	20.414276732771782
17	22.718651434488663	25.15422668284513	29.054140918192495	23.07298096447371
18	24.087892038680387	24.986043431783642	30.466704118992734	20.45936041054324
19	23.80629516072507	27.132096936951534	27.95716346179246	21.10444444053093
20	23.931331923294348	27.515132089892624	28.72587538941907	19.827660597393955
21	23.501628119535145	27.583109822782404	29.55728180644661	19.357980251235837
22	21.888477774275184	24.835823208584216	29.502512182278945	23.773186834861658
23	21.58539570613191	26.157866837608946	30.026433828255843	22.230303628003302
24	22.29458309045089	25.45272993996474	29.611699214381694	22.640987755202673
25	21.651964574091327	26.631245454209246	28.163209957857326	23.553580013842097
26	20.553226036528347	26.906326331861656	29.77371868137287	22.76672895023713
27	21.26804888056876	26.873218005998247	29.348417580521037	22.510315532911964
28	19.150348782124322	27.123995963601978	30.342724005121223	23.38293124915248
29	21.253431906916294	25.76021471101539	30.73685396938889	22.249499412679434
30	21.573772570456455	26.574010316413453	30.483610498157027	21.368606614973064
31	22.368372391178394	27.603538364272595	28.006121518122406	22.02196772642661
32	23.087950154358765	26.949648928470165	27.99379394998177	21.968606967189295
33	22.07127799898914	26.324993439972665	28.67991117106614	22.92381738997205
34	20.45812765372918	25.406941829728108	29.982406799182154	24.152523717360562
35	20.78480820945595	25.620913191026233	29.81369522377178	23.780583375746037
36	20.650966041071936	27.6403449605782	30.073983019655426	21.63470597869444
37	20.685835448098295	28.136441524180523	28.528986515401535	22.64873651231964
38	20.65343155470006	27.909085946043994	28.413107374879587	23.024375124376355
39	21.548765217942602	24.370017240984584	29.673337055084858	24.407880485987956
40	22.182930544720016	23.02455123249265	30.824379703187383	23.96813851959995
41	19.06511245383766	25.074801922396194	29.570313807052422	26.28977181671372
42	20.552169387830578	25.24985338999318	30.125934913962382	24.07204230821386
43	21.329334505039334	25.180819008405642	29.993501610508723	23.4963448760463
44	21.388330723998077	24.933210996895212	29.298755091725916	24.379703187380798
45	21.629598843321894	24.039990631048212	28.89511528917833	25.43529523645156
46	22.119707730970198	24.88231575128603	29.19432297876312	23.80365353898065
47	19.080257751839007	25.521412105319698	31.08766133704804	24.310668805793252
48	19.4902374465732	24.936909267337406	31.10773766230564	24.465115623783753
49	21.921586100138597	23.31671459742565	31.77853347727237	22.983165825163386
50	20.298925916598716	23.190268969926016	31.265530534505743	25.24527457896952
51	19.590795180977505	23.07632701868331	29.774951438186932	27.55792636215225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	243.0
1	472.5
2	702.0
3	1976.0
4	3250.0
5	2092.5
6	935.0
7	955.0
8	975.0
9	983.0
10	991.0
11	973.0
12	955.0
13	1027.5
14	1100.0
15	1028.5
16	957.0
17	963.0
18	969.0
19	1064.5
20	1160.0
21	1409.0
22	1658.0
23	1923.5
24	2189.0
25	2694.0
26	4224.0
27	5249.0
28	6087.0
29	6925.0
30	8179.0
31	9433.0
32	10351.0
33	11269.0
34	13382.0
35	15495.0
36	17138.0
37	18781.0
38	20957.5
39	23134.0
40	26075.5
41	29017.0
42	32709.5
43	36402.0
44	44089.5
45	51777.0
46	55553.0
47	59329.0
48	59492.0
49	59655.0
50	52412.0
51	45169.0
52	40360.5
53	35552.0
54	32682.0
55	29812.0
56	27060.0
57	24308.0
58	23243.0
59	22178.0
60	20313.5
61	18449.0
62	16755.0
63	15061.0
64	12755.0
65	10449.0
66	9001.5
67	7554.0
68	6425.5
69	5297.0
70	4389.5
71	3482.0
72	2758.0
73	2034.0
74	1698.0
75	1045.0
76	728.0
77	540.0
78	352.0
79	264.0
80	176.0
81	131.5
82	87.0
83	53.0
84	19.0
85	13.0
86	7.0
87	6.0
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	567833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.420885759512316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.13393480341696	25.550478640559422
2	10.249909167036778	7.261217233019755
3	3.7365378355985683	3.97054439432497
4	2.0043009653946333	2.8397646209169425
5	1.3531340273364652	2.396460289979687
6	1.073076792389268	2.280559828464251
7	0.8610596041550042	2.134964570963371
8	0.7891138541638478	2.236088934366889
9	0.6876345125100176	2.192096116072375
>10	7.016991940760119	44.163087616955195
>50	0.05869074562180952	1.4123933998710654
>100	0.03059944857198952	1.7459023206831334
>500	0.003009781826753068	0.7434210573720975
>1k	0.0020065212178353785	1.073020976450788
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2060	0.3627827195671967	No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	1753	0.3087175278647067	No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	1219	0.2146757937633072	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC	1007	0.17734087310881896	No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	950	0.16730271048001788	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGC	805	0.14176703361727833	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCG	787	0.1385970875239727	No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	630	0.11094811326569608	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.522162325895114E-4	0.0	0.0	0.1717054133873868	0.0
2	3.522162325895114E-4	0.0	0.0	0.8759617704501147	0.0
3	3.522162325895114E-4	0.0	0.0	1.2362789763891848	0.0
4	3.522162325895114E-4	0.0	0.0	1.7175824582227521	0.0
5	3.522162325895114E-4	0.0	0.0	3.0998550630202892	0.0
6	3.522162325895114E-4	0.0	0.0	3.9553882919802126	0.0
7	3.522162325895114E-4	0.0	0.0	4.744352652980718	0.0
8	3.522162325895114E-4	0.0	0.0	5.997890224766789	0.0
9	3.522162325895114E-4	0.0	0.0	6.447318137551005	0.0
10	3.522162325895114E-4	0.0	0.0	7.581982730838116	0.0
11	3.522162325895114E-4	0.0	0.0	9.072385719040634	0.0
12	3.522162325895114E-4	0.0	0.0	10.345999616084306	0.0
13	3.522162325895114E-4	0.0	0.0	10.862524721176825	0.0
14	3.522162325895114E-4	0.0	0.0	11.067690676660215	0.0
15	3.522162325895114E-4	0.0	0.0	11.466575560067836	0.0
16	3.522162325895114E-4	0.0	0.0	12.255716029184637	0.0
17	3.522162325895114E-4	0.0	0.0	13.17676147740621	0.0
18	3.522162325895114E-4	0.0	0.0	14.19924520061356	0.0
19	3.522162325895114E-4	0.0	0.0	14.81174922908672	0.0
20	5.28324348884267E-4	0.0	0.0	15.409284067674827	0.0
21	5.28324348884267E-4	0.0	0.0	16.140484966530654	0.0
22	5.28324348884267E-4	0.0	0.0	16.924870516507493	0.0
23	5.28324348884267E-4	0.0	0.0	17.692878011668924	0.0
24	5.28324348884267E-4	0.0	0.0	18.302035985932484	0.0
25	5.28324348884267E-4	0.0	0.0	18.856600444144668	0.0
26	5.28324348884267E-4	0.0	0.0	19.36432014342245	0.0
27	5.28324348884267E-4	0.0	0.0	19.90726146595918	0.0
28	5.28324348884267E-4	0.0	0.0	20.464291437799496	0.0
29	5.28324348884267E-4	0.0	0.0	20.997546813940016	0.0
30	5.28324348884267E-4	0.0	0.0	21.633825438112968	0.0
31	5.28324348884267E-4	0.0	0.0	22.198780275186543	0.0
32	5.28324348884267E-4	0.0	0.0	22.755458030794266	0.0
33	7.044324651790228E-4	0.0	0.0	23.306676434796852	0.0
34	7.044324651790228E-4	0.0	0.0	23.829013107727096	0.0
35	7.044324651790228E-4	0.0	0.0	24.41386816194198	0.0
36	7.044324651790228E-4	0.0	0.0	24.948004078663974	0.0
37	7.044324651790228E-4	0.0	0.0	25.471397400291988	0.0
38	7.044324651790228E-4	0.0	0.0	26.035823913016678	0.0
39	7.044324651790228E-4	0.0	0.0	26.637761454512155	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGCA	20	7.030269E-4	45.0	41
TTACGTT	20	7.030269E-4	45.0	25
ACGTAAG	20	7.030269E-4	45.0	1
TCACGAC	30	2.163555E-6	44.999996	25
TCTACGG	30	2.163555E-6	44.999996	2
TACGCGG	45	1.9254003E-8	40.0	2
AATTGCG	45	1.9254003E-8	40.0	1
GGCGATA	145	0.0	38.793102	8
ACCGGGT	35	6.242706E-6	38.571426	4
GCGAACG	35	6.242706E-6	38.571426	1
CACGACG	35	6.242706E-6	38.571426	26
TAGCCGT	35	6.242706E-6	38.571426	44
GCGATTA	35	6.242706E-6	38.571426	9
GTTCACG	35	6.242706E-6	38.571426	1
CGTTTTT	950	0.0	37.894737	1
CGTTAGG	90	0.0	37.5	2
CGTAAGG	90	0.0	37.5	2
CGCATCG	30	1.13919465E-4	37.499996	21
ATTCGCA	30	1.13919465E-4	37.499996	13
TAACGCC	30	1.13919465E-4	37.499996	12
>>END_MODULE