SRR2935500.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935500.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	521588
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	12958	2.484336296080431	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	12312	2.360483753460586	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	10732	2.0575626739878983	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3938	0.7550020322553432	No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	3844	0.7369801452487403	TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	2807	0.5381642215695146	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	2277	0.43655145440462584	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCCACTTT	2239	0.42926601072110554	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	1899	0.3640804619738184	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTA	1875	0.3594791291210687	No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	791	0.15165226193854153	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	769	0.14743437349018765	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	758	0.14532542926601072	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG	740	0.14187442962644845	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	652	0.125002875833033	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATG	597	0.11445815471214829	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGT	539	0.1033382669846699	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCTA	25	3.8878647E-5	45.000004	30
CGACGGT	45	3.8380676E-10	45.000004	28
CACGACG	45	3.8380676E-10	45.000004	26
TCGATAG	25	3.8878647E-5	45.000004	1
CGGTCTA	45	3.8380676E-10	45.000004	31
CGCAACC	50	2.1827873E-11	45.000004	43
GCGTAAG	50	2.1827873E-11	45.000004	1
GTATGCG	20	7.0297404E-4	45.0	1
TAATACG	30	2.1632131E-6	44.999996	4
CGATGAA	1575	0.0	43.285713	19
GATGAAT	1625	0.0	43.2	20
CCGATGA	1580	0.0	43.148735	18
CGTAAGG	80	0.0	42.1875	2
CACCGAT	1690	0.0	42.071007	16
CGGCGAG	345	0.0	41.739132	31
CGTTTTT	1515	0.0	41.73267	1
TACGGCT	1255	0.0	41.593624	7
ACGGCTG	1265	0.0	41.26482	8
ATGAATG	1685	0.0	41.261127	21
CGATCAC	60	3.6379788E-12	41.249996	34