FastQCFastQC Report
Sat 14 Jan 2017
SRR2935499.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935499.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675889
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC183152.7097644731605337No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124911.848084522754476No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC93871.3888375162193793No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG91931.3601345783109355No Hit
GAACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT54740.8098962995403091No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC46350.6857634907507002No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT30460.4506657158202012No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT23660.350057479852461No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT22980.3399966562556869No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC21370.3161761768574426No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG21280.3148445972637519No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCAAAGT19060.28199896728604845No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC17250.25521942212404697No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTA15070.22296560529909498No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT14420.21334864156688452No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT12160.17991119843642966No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT11080.16393224331214148No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC10590.15668253219093667No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC8280.12250532261954256No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC6900.10208776884961879No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG207.0312206E-445.0000041
CGTCGAA351.2109376E-745.042
TTCGACG253.889094E-545.01
AGTTACG302.16417E-644.9999961
CGTTTTT54050.043.66791
CGATGAA12550.043.56573519
CCGATGA12600.042.67857418
GTCGAAT1700.042.3529443
CACCGAT13450.041.8215616
CGACGGT700.041.78571328
CGTTTTC1800.041.251
GATGAAT13750.040.90909220
TACGGCT11600.040.538797
CTCGTAA501.0804797E-940.533
TCGTAAC501.0804797E-940.534
GCTCGTA501.0804797E-940.532
ACCGATG13400.040.29850417
CGGCGAA1850.040.13513631
CCGTCGA451.9263098E-840.041
GCGATCT451.9263098E-840.09
Produced by FastQC (version 0.11.5)